Restriction map for
pF1KB8470
(Linear) (Six-base) MAPSORT of: KIBB8470.seq Check: 4903 from: 1 to: 2466
KIBB8470.orf
With 182 enzymes: SgfI *
July 28, 2010 10:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 652 2466
Size: 652 1814
AatII G_ACGT'C
Cuts at: 0 1265 2207 2466
Size: 1265 942 259
Fragments arranged by size:
1265 942 259
AccI GT'mk_AC
Cuts at: 0 1994 2447 2466
Size: 1994 453 19
Fragments arranged by size:
1994 453 19
Acc65I G'GTAC_C
Cuts at: 0 2261 2466
Size: 2261 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1741 2404 2466
Size: 928 813 663 62
Fragments arranged by size:
928 813 663 62
AfeI AGC'GCT
Cuts at: 0 941 2466
Size: 941 1525
AflII C'TTAA_G
Cuts at: 0 747 1360 2466
Size: 747 613 1106
Fragments arranged by size:
1106 747 613
AflIII A'CryG_T
Cuts at: 0 1002 2099 2466
Size: 1002 1097 367
Fragments arranged by size:
1097 1002 367
AgeI A'CCGG_T
Cuts at: 0 1276 2466
Size: 1276 1190
AlwNI CAG_nnn'CTG
Cuts at: 0 1498 1959 2042 2466
Size: 1498 461 83 424
Fragments arranged by size:
1498 461 424 83
ApaLI G'TGCA_C
Cuts at: 0 2017 2466
Size: 2017 449
ApoI r'AATT_y
Cuts at: 0 209 310 2185 2466
Size: 209 101 1875 281
Fragments arranged by size:
1875 281 209 101
AvaI C'yCGr_G
Cuts at: 0 2093 2466
Size: 2093 373
BanI G'GyrC_C
Cuts at: 0 2261 2466
Size: 2261 205
BanII G_rGCy'C
Cuts at: 0 745 1772 2381 2466
Size: 745 1027 609 85
Fragments arranged by size:
1027 745 609 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 1720 2466
Size: 1720 746
BbvCI CC'TCA_GC
Cuts at: 0 1919 2466
Size: 1919 547
BglII A'GATC_T
Cuts at: 0 788 1697 2466
Size: 788 909 769
Fragments arranged by size:
909 788 769
Bme1580I G_kGCm'C
Cuts at: 0 1893 2021 2335 2466
Size: 1893 128 314 131
Fragments arranged by size:
1893 314 131 128
BmgBI CAC'GTC
Cuts at: 0 101 2100 2466
Size: 101 1999 366
Fragments arranged by size:
1999 366 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 1536 2466
Size: 1536 930
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1484 2466
Size: 1484 982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1021 1768 2147 2229 2466
Size: 96 925 747 379 82 237
Fragments arranged by size:
925 747 379 237 96 82
Bpu10I CC'TnA_GC
Cuts at: 0 1685 1919 2466
Size: 1685 234 547
Fragments arranged by size:
1685 547 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 2466
Size: 1555 911
BsaAI yAC'GTr
Cuts at: 0 2330 2466
Size: 2330 136
BsaHI Gr'CG_yC
Cuts at: 0 1262 1814 2002 2204 2466
Size: 1262 552 188 202 262
Fragments arranged by size:
1262 552 262 202 188
BsaWI w'CCGG_w
Cuts at: 0 669 1276 2442 2466
Size: 669 607 1166 24
Fragments arranged by size:
1166 669 607 24
BseYI C'CCAG_C
Cuts at: 0 1398 1970 2006 2358 2466
Size: 1398 572 36 352 108
Fragments arranged by size:
1398 572 352 108 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2466
Size: 153 2313
BsiEI CG_ry'CG
Cuts at: 0 1281 2466
Size: 1281 1185
BsiHKAI G_wGCw'C
Cuts at: 0 1772 1796 2021 2466
Size: 1772 24 225 445
Fragments arranged by size:
1772 445 225 24
BsmI GAATG_Cn'
Cuts at: 0 905 1672 2466
Size: 905 767 794
Fragments arranged by size:
905 794 767
Bsp1286I G_dGCh'C
Cuts at: 0 745 1772 1796 1893 2021 2335 2381 2466
Size: 745 1027 24 97 128 314 46 85
Fragments arranged by size:
1027 745 314 128 97 85 46 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 572 652 2466
Size: 356 216 80 1814
Fragments arranged by size:
1814 356 216 80
BsrDI GCAATG_nn'
Cuts at: 0 1422 2466
Size: 1422 1044
BsrFI r'CCGG_y
Cuts at: 0 1276 2466
Size: 1276 1190
BsrGI T'GTAC_A
Cuts at: 0 2102 2177 2466
Size: 2102 75 289
Fragments arranged by size:
2102 289 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1959 2016 2466
Size: 1959 57 450
Fragments arranged by size:
1959 450 57
BstEII G'GTnAC_C
Cuts at: 0 697 2466
Size: 697 1769
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1549 2466
Size: 1549 917
BstYI r'GATC_y
Cuts at: 0 125 189 788 1520 1697 2466
Size: 125 64 599 732 177 769
Fragments arranged by size:
769 732 599 177 125 64
Bsu36I CC'TnA_GG
Cuts at: 0 1885 2466
Size: 1885 581
BtgI C'CryG_G
Cuts at: 0 32 1026 1934 1982 2466
Size: 32 994 908 48 484
Fragments arranged by size:
994 908 484 48 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 201 2466
Size: 201 2265
BtsI GCAGTG_nn'
Cuts at: 0 120 1715 2466
Size: 120 1595 751
Fragments arranged by size:
1595 751 120
DraIII CAC_nnn'GTG
Cuts at: 0 2016 2466
Size: 2016 450
DrdI GACnn_nn'nnGTC
Cuts at: 0 387 2466
Size: 387 2079
EaeI y'GGCC_r
Cuts at: 0 602 2030 2130 2429 2466
Size: 602 1428 100 299 37
Fragments arranged by size:
1428 602 299 100 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 2466
Size: 817 1649
EcoICRI GAG'CTC
Cuts at: 0 1770 2466
Size: 1770 696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 928 1021 1741 1768 2147 2229 2404
Size: 96 832 93 720 27 379 82 175
Cuts at: 2404 2466
Size: 62
Fragments arranged by size:
832 720 379 175 96 93 82 62
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12 2466
Size: 12 2454
EcoO109I rG'GnC_Cy
Cuts at: 0 1869 2466
Size: 1869 597
HaeII r_GCGC'y
Cuts at: 0 943 2466
Size: 943 1523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 481 497 513 1110 1142 1411 1443
Size: 465 16 16 16 597 32 269 32
Cuts at: 1443 2466
Size: 1023
Fragments arranged by size:
1023 597 465 269 32 32 16 16
16
HincII GTy'rAC
Cuts at: 0 837 2466
Size: 837 1629
HindIII A'AGCT_T
Cuts at: 0 263 2466
Size: 263 2203
KpnI G_GTAC'C
Cuts at: 0 2265 2466
Size: 2265 201
MfeI C'AATT_G
Cuts at: 0 290 576 2466
Size: 290 286 1890
Fragments arranged by size:
1890 290 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 2466
Size: 907 1559
MscI TGG'CCA
Cuts at: 0 2032 2132 2431 2466
Size: 2032 100 299 35
Fragments arranged by size:
2032 299 100 35
MslI CAynn'nnrTG
Cuts at: 0 450 719 1057 2466
Size: 450 269 338 1409
Fragments arranged by size:
1409 450 338 269
MspA1I CmG'CkG
Cuts at: 0 1041 1984 2268 2466
Size: 1041 943 284 198
Fragments arranged by size:
1041 943 284 198
NcoI C'CATG_G
Cuts at: 0 32 2466
Size: 32 2434
NdeI CA'TA_TG
Cuts at: 0 716 2466
Size: 716 1750
NspI r_CATG'y
Cuts at: 0 455 1006 2466
Size: 455 551 1460
Fragments arranged by size:
1460 551 455
PciI A'CATG_T
Cuts at: 0 1002 2466
Size: 1002 1464
PflMI CCAn_nnn'nTGG
Cuts at: 0 2153 2466
Size: 2153 313
PfoI T'CCnGG_A
Cuts at: 0 7 2466
Size: 7 2459
PmlI CAC'GTG
Cuts at: 0 2330 2466
Size: 2330 136
PpuMI rG'GwC_Cy
Cuts at: 0 1869 2466
Size: 1869 597
PstI C_TGCA'G
Cuts at: 0 353 569 1209 2318 2466
Size: 353 216 640 1109 148
Fragments arranged by size:
1109 640 353 216 148
PvuII CAG'CTG
Cuts at: 0 1041 2268 2466
Size: 1041 1227 198
Fragments arranged by size:
1227 1041 198
SacI G_AGCT'C
Cuts at: 0 1772 2466
Size: 1772 694
SacII CC_GC'GG
Cuts at: 0 1985 2466
Size: 1985 481
ScaI AGT'ACT
Cuts at: 0 1546 2466
Size: 1546 920
SexAI A'CCwGG_T
Cuts at: 0 701 2466
Size: 701 1765
SfcI C'TryA_G
Cuts at: 0 349 565 651 1205 2118 2314 2466
Size: 349 216 86 554 913 196 152
Fragments arranged by size:
913 554 349 216 196 152 86
SmaI CCC'GGG
Cuts at: 0 2095 2466
Size: 2095 371
SmlI C'TyrA_G
Cuts at: 0 747 1360 1570 2466
Size: 747 613 210 896
Fragments arranged by size:
896 747 613 210
SspI AAT'ATT
Cuts at: 0 549 968 2466
Size: 549 419 1498
Fragments arranged by size:
1498 549 419
StyI C'CwwG_G
Cuts at: 0 32 2432 2466
Size: 32 2400 34
Fragments arranged by size:
2400 34 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1284 2466
Size: 1284 1182
TatI w'GTAC_w
Cuts at: 0 521 991 1544 1853 2102 2177 2466
Size: 521 470 553 309 249 75 289
Fragments arranged by size:
553 521 470 309 289 249 75
Tth111I GACn'n_nGTC
Cuts at: 0 1947 2466
Size: 1947 519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 944 2043 2466
Size: 944 1099 423
Fragments arranged by size:
1099 944 423
XmaI C'CCGG_G
Cuts at: 0 2093 2466
Size: 2093 373
XmnI GAAnn'nnTTC
Cuts at: 0 400 2466
Size: 400 2066
ZraI GAC'GTC
Cuts at: 0 1263 2205 2466
Size: 1263 942 261
Fragments arranged by size:
1263 942 261
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NspI PciI PflMI PfoI PmlI PpuMI
PstI PvuII SacI SacII ScaI SexAI SfcI SmaI
SmlI SspI StyI TaqII TatI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BsaI BsaBI BsaXI BseRI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI EagI EarI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XhoI