Restriction map for
pF1KB8471
(Linear) (Six-base) MAPSORT of: KIBB8471.seq Check: 8818 from: 1 to: 2736
KIBB8471.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1110 1655 2736
Size: 1110 545 1081
Fragments arranged by size:
1110 1081 545
AatII G_ACGT'C
Cuts at: 0 2060 2630 2736
Size: 2060 570 106
Fragments arranged by size:
2060 570 106
Acc65I G'GTAC_C
Cuts at: 0 1768 2736
Size: 1768 968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 986 1969 2734 2736
Size: 398 588 983 765 2
Fragments arranged by size:
983 765 588 398 2
AflIII A'CryG_T
Cuts at: 0 1313 1751 2736
Size: 1313 438 985
Fragments arranged by size:
1313 985 438
AlwNI CAG_nnn'CTG
Cuts at: 0 2126 2661 2736
Size: 2126 535 75
Fragments arranged by size:
2126 535 75
ApaI G_GGCC'C
Cuts at: 0 342 698 1230 2098 2373 2736
Size: 342 356 532 868 275 363
Fragments arranged by size:
868 532 363 356 342 275
ApaLI G'TGCA_C
Cuts at: 0 2580 2736
Size: 2580 156
ApoI r'AATT_y
Cuts at: 0 1003 1846 2736
Size: 1003 843 890
Fragments arranged by size:
1003 890 843
AvaI C'yCGr_G
Cuts at: 0 25 497 697 2317 2736
Size: 25 472 200 1620 419
Fragments arranged by size:
1620 472 419 200 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1752 1785 2736
Size: 1752 33 951
Fragments arranged by size:
1752 951 33
BanI G'GyrC_C
Cuts at: 0 247 970 1768 2027 2736
Size: 247 723 798 259 709
Fragments arranged by size:
798 723 709 259 247
BanII G_rGCy'C
Cuts at: 0 122 296 316 342 435 496 698 791
Size: 122 174 20 26 93 61 202 93
Cuts at: 791 1045 1230 1940 2098 2373 2736
Size: 254 185 710 158 275 363
Fragments arranged by size:
710 363 275 254 202 185 174 158
122 93 93 61 26 20
BbeI G_GCGC'C
Cuts at: 0 251 2736
Size: 251 2485
BbsI GAAGACnn'nnnn_
Cuts at: 0 1739 2541 2736
Size: 1739 802 195
Fragments arranged by size:
1739 802 195
BglI GCCn_nnn'nGGC
Cuts at: 0 1259 1809 2736
Size: 1259 550 927
Fragments arranged by size:
1259 927 550
BlpI GC'TnA_GC
Cuts at: 0 1039 2736
Size: 1039 1697
Bme1580I G_kGCm'C
Cuts at: 0 342 698 1230 1763 2098 2179 2373 2584
Size: 342 356 532 533 335 81 194 211
Cuts at: 2584 2736
Size: 152
Fragments arranged by size:
533 532 356 342 335 211 194 152
81
BmrI ACTGGGnnnn_n'
Cuts at: 0 2605 2736
Size: 2605 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1585 1798 2458 2476 2736
Size: 1285 300 213 660 18 260
Fragments arranged by size:
1285 660 300 260 213 18
Bpu10I CC'TnA_GC
Cuts at: 0 785 2736
Size: 785 1951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 2736
Size: 369 2367
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 457 2736
Size: 361 96 2279
Fragments arranged by size:
2279 361 96
BsaAI yAC'GTr
Cuts at: 0 1073 2736
Size: 1073 1663
BsaHI Gr'CG_yC
Cuts at: 0 248 2057 2627 2736
Size: 248 1809 570 109
Fragments arranged by size:
1809 570 248 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 871 901 1558 1588 1789 1819 2736
Size: 871 30 657 30 201 30 917
Fragments arranged by size:
917 871 657 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 94 103 121 319 443 733 2736
Size: 73 21 9 18 198 124 290 2003
Fragments arranged by size:
2003 290 198 124 73 21 18 9
BseYI C'CCAG_C
Cuts at: 0 121 296 319 342 524 805 977 1878
Size: 121 175 23 23 182 281 172 901
Cuts at: 1878 2664 2736
Size: 786 72
Fragments arranged by size:
901 786 281 182 175 172 121 72
23 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 393 760 1222 1593 2345 2736
Size: 380 13 367 462 371 752 391
Fragments arranged by size:
752 462 391 380 371 367 13
BsiEI CG_ry'CG
Cuts at: 0 861 2736
Size: 861 1875
BsiHKAI G_wGCw'C
Cuts at: 0 1045 1381 1640 2584 2736
Size: 1045 336 259 944 152
Fragments arranged by size:
1045 944 336 259 152
BsmI GAATG_Cn'
Cuts at: 0 678 822 1961 2736
Size: 678 144 1139 775
Fragments arranged by size:
1139 775 678 144
BsmBI CGTCTCn'nnnn_
Cuts at: 0 989 2634 2736
Size: 989 1645 102
Fragments arranged by size:
1645 989 102
Bsp1286I G_dGCh'C
Cuts at: 0 122 296 316 342 435 496 698 791
Size: 122 174 20 26 93 61 202 93
Cuts at: 791 1045 1230 1381 1640 1763 1940 2098 2179
Size: 254 185 151 259 123 177 158 81
Cuts at: 2179 2373 2584 2736
Size: 194 211 152
Fragments arranged by size:
259 254 211 202 194 185 177 174
158 152 151 123 122 93 93 81
61 26 20
BspHI T'CATG_A
Cuts at: 0 2552 2736
Size: 2552 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1110 1655 2736
Size: 1110 545 1081
Fragments arranged by size:
1110 1081 545
BsrBI CCG'CTC
Cuts at: 0 54 1431 2736
Size: 54 1377 1305
Fragments arranged by size:
1377 1305 54
BsrDI GCAATG_nn'
Cuts at: 0 659 1979 2136 2736
Size: 659 1320 157 600
Fragments arranged by size:
1320 659 600 157
BsrFI r'CCGG_y
Cuts at: 0 503 2183 2669 2736
Size: 503 1680 486 67
Fragments arranged by size:
1680 503 486 67
BsrGI T'GTAC_A
Cuts at: 0 1304 2736
Size: 1304 1432
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1556 1616 2126 2661 2736
Size: 1556 60 510 535 75
Fragments arranged by size:
1556 535 510 75 60
BstEII G'GTnAC_C
Cuts at: 0 2386 2736
Size: 2386 350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 1194 1249 2013 2736
Size: 486 708 55 764 723
Fragments arranged by size:
764 723 708 486 55
Bsu36I CC'TnA_GG
Cuts at: 0 1256 1328 2736
Size: 1256 72 1408
Fragments arranged by size:
1408 1256 72
BtgI C'CryG_G
Cuts at: 0 128 230 791 1193 2279 2736
Size: 128 102 561 402 1086 457
Fragments arranged by size:
1086 561 457 402 128 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 848 1184 2736
Size: 848 336 1552
Fragments arranged by size:
1552 848 336
BtsI GCAGTG_nn'
Cuts at: 0 398 1059 1181 2736
Size: 398 661 122 1555
Fragments arranged by size:
1555 661 398 122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 374 409 2736
Size: 374 35 2327
Fragments arranged by size:
2327 374 35
EaeI y'GGCC_r
Cuts at: 0 858 916 1124 2181 2276 2736
Size: 858 58 208 1057 95 460
Fragments arranged by size:
1057 858 460 208 95 58
EagI C'GGCC_G
Cuts at: 0 858 2736
Size: 858 1878
EarI CTCTTCn'nnn_
Cuts at: 0 1274 1369 1528 1882 2194 2707 2736
Size: 1274 95 159 354 312 513 29
Fragments arranged by size:
1274 513 354 312 159 95 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2683 2736
Size: 2683 53
EcoICRI GAG'CTC
Cuts at: 0 1043 2736
Size: 1043 1693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 986 1285 1585 1798 1969 2458 2476
Size: 398 588 299 300 213 171 489 18
Cuts at: 2476 2734 2736
Size: 258 2
Fragments arranged by size:
588 489 398 300 299 258 213 171
18 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2170 2736
Size: 2170 566
EcoO109I rG'GnC_Cy
Cuts at: 0 201 338 339 579 694 1113 1227 1260
Size: 201 137 1 240 115 419 114 33
Cuts at: 1260 2094 2163 2369 2736
Size: 834 69 206 367
Fragments arranged by size:
834 419 367 240 206 201 137 115
114 69 33 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1575 2736
Size: 1575 1161
HaeII r_GCGC'y
Cuts at: 0 114 251 2736
Size: 114 137 2485
Fragments arranged by size:
2485 137 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 1789 1821 2736
Size: 745 32 1012 32 915
Fragments arranged by size:
1012 915 745 32 32
HincII GTy'rAC
Cuts at: 0 2148 2736
Size: 2148 588
KasI G'GCGC_C
Cuts at: 0 247 2736
Size: 247 2489
KpnI G_GTAC'C
Cuts at: 0 1772 2736
Size: 1772 964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1509 2348 2657 2736
Size: 1509 839 309 79
Fragments arranged by size:
1509 839 309 79
MscI TGG'CCA
Cuts at: 0 918 1126 2278 2736
Size: 918 208 1152 458
Fragments arranged by size:
1152 918 458 208
MslI CAynn'nnrTG
Cuts at: 0 484 1312 1318 1903 1966 2011 2587 2736
Size: 484 828 6 585 63 45 576 149
Fragments arranged by size:
828 585 576 484 149 63 45 6
MspA1I CmG'CkG
Cuts at: 0 20 364 546 964 977 1038 1336 1384
Size: 20 344 182 418 13 61 298 48
Cuts at: 1384 1882 2359 2712 2736
Size: 498 477 353 24
Fragments arranged by size:
498 477 418 353 344 298 182 61
48 24 20 13
NaeI GCC'GGC
Cuts at: 0 505 2185 2736
Size: 505 1680 551
Fragments arranged by size:
1680 551 505
NarI GG'CG_CC
Cuts at: 0 248 2736
Size: 248 2488
NcoI C'CATG_G
Cuts at: 0 230 2279 2736
Size: 230 2049 457
Fragments arranged by size:
2049 457 230
NgoMIV G'CCGG_C
Cuts at: 0 503 2183 2736
Size: 503 1680 553
Fragments arranged by size:
1680 553 503
NruI TCG'CGA
Cuts at: 0 2310 2736
Size: 2310 426
NspI r_CATG'y
Cuts at: 0 1317 1755 2736
Size: 1317 438 981
Fragments arranged by size:
1317 981 438
PasI CC'CwG_GG
Cuts at: 0 1230 2736
Size: 1230 1506
PciI A'CATG_T
Cuts at: 0 1313 1751 2736
Size: 1313 438 985
Fragments arranged by size:
1313 985 438
PfoI T'CCnGG_A
Cuts at: 0 2084 2736
Size: 2084 652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1505 1537 2736
Size: 1505 32 1199
Fragments arranged by size:
1505 1199 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 579 1113 2736
Size: 201 378 534 1623
Fragments arranged by size:
1623 534 378 201
PspOMI G'GGCC_C
Cuts at: 0 338 694 1226 2094 2369 2736
Size: 338 356 532 868 275 367
Fragments arranged by size:
868 532 367 356 338 275
PstI C_TGCA'G
Cuts at: 0 441 1121 1666 2447 2736
Size: 441 680 545 781 289
Fragments arranged by size:
781 680 545 441 289
PvuII CAG'CTG
Cuts at: 0 364 964 1336 1882 2359 2736
Size: 364 600 372 546 477 377
Fragments arranged by size:
600 546 477 377 372 364
SacI G_AGCT'C
Cuts at: 0 1045 2736
Size: 1045 1691
SanDI GG'GwC_CC
Cuts at: 0 579 2736
Size: 579 2157
SapI GCTCTTCn'nnn_
Cuts at: 0 1369 2194 2736
Size: 1369 825 542
Fragments arranged by size:
1369 825 542
SbfI CC_TGCA'GG
Cuts at: 0 1121 2736
Size: 1121 1615
SfcI C'TryA_G
Cuts at: 0 437 1117 1662 1931 2443 2736
Size: 437 680 545 269 512 293
Fragments arranged by size:
680 545 512 437 293 269
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1259 2736
Size: 1259 1477
SfoI GGC'GCC
Cuts at: 0 249 2736
Size: 249 2487
SmaI CCC'GGG
Cuts at: 0 27 499 699 2736
Size: 27 472 200 2037
Fragments arranged by size:
2037 472 200 27
SmlI C'TyrA_G
Cuts at: 0 384 2317 2736
Size: 384 1933 419
Fragments arranged by size:
1933 419 384
StyI C'CwwG_G
Cuts at: 0 230 416 653 1187 1394 2228 2279 2736
Size: 230 186 237 534 207 834 51 457
Fragments arranged by size:
834 534 457 237 230 207 186 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 1308 2736
Size: 80 1228 1428
Fragments arranged by size:
1428 1228 80
TatI w'GTAC_w
Cuts at: 0 1304 2736
Size: 1304 1432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 871 903 2736
Size: 871 32 1833
Fragments arranged by size:
1833 871 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 2736
Size: 273 2463
XhoI C'TCGA_G
Cuts at: 0 2317 2736
Size: 2317 419
XmaI C'CCGG_G
Cuts at: 0 25 497 697 2736
Size: 25 472 200 2039
Fragments arranged by size:
2039 472 200 25
XmnI GAAnn'nnTTC
Cuts at: 0 1147 2736
Size: 1147 1589
ZraI GAC'GTC
Cuts at: 0 2058 2628 2736
Size: 2058 570 108
Fragments arranged by size:
2058 570 108
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbsI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI SfcI SfiI SfoI SmaI
SmlI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI AvrII BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaBI
BsaWI BsiWI BspEI BssHII BssSI BstBI BstYI BstZ17I
ClaI DraI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NsiI PacI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI