Restriction map for
pF1KB8472
(Linear) (Six-base) MAPSORT of: KIBB8472.seq Check: 8201 from: 1 to: 2520
KIBB8472.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 0 602 2520
Size: 602 1918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 827 1250 1658 2096 2520
Size: 394 433 423 408 438 424
Fragments arranged by size:
438 433 424 423 408 394
AflII C'TTAA_G
Cuts at: 0 397 2520
Size: 397 2123
AflIII A'CryG_T
Cuts at: 0 1587 2520
Size: 1587 933
AhdI GACnn_n'nnGTC
Cuts at: 0 768 2520
Size: 768 1752
AleI CACnn'nnGTG
Cuts at: 0 731 2473 2520
Size: 731 1742 47
Fragments arranged by size:
1742 731 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 368 2520
Size: 368 2152
AlwNI CAG_nnn'CTG
Cuts at: 0 1369 1636 2520
Size: 1369 267 884
Fragments arranged by size:
1369 884 267
ApoI r'AATT_y
Cuts at: 0 330 703 739 913 1066 1171 1218 2520
Size: 330 373 36 174 153 105 47 1302
Fragments arranged by size:
1302 373 330 174 153 105 47 36
AseI AT'TA_AT
Cuts at: 0 508 2520
Size: 508 2012
AvaI C'yCGr_G
Cuts at: 0 1118 2520
Size: 1118 1402
BamHI G'GATC_C
Cuts at: 0 1663 2277 2520
Size: 1663 614 243
Fragments arranged by size:
1663 614 243
BbsI GAAGACnn'nnnn_
Cuts at: 0 1723 1997 2520
Size: 1723 274 523
Fragments arranged by size:
1723 523 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1053 1087 2520
Size: 1053 34 1433
Fragments arranged by size:
1433 1053 34
BclI T'GATC_A
Cuts at: 0 1811 2169 2520
Size: 1811 358 351
Fragments arranged by size:
1811 358 351
BglI GCCn_nnn'nGGC
Cuts at: 0 1705 2520
Size: 1705 815
BglII A'GATC_T
Cuts at: 0 846 2520
Size: 846 1674
BlpI GC'TnA_GC
Cuts at: 0 2453 2520
Size: 2453 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1795 2520
Size: 1795 725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1208 1404 1849 2520
Size: 940 268 196 445 671
Fragments arranged by size:
940 671 445 268 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1345 1640 2520
Size: 802 543 295 880
Fragments arranged by size:
880 802 543 295
BsaI GGTCTCn'nnnn_
Cuts at: 0 1642 2520
Size: 1642 878
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2149 2179 2520
Size: 2149 30 341
Fragments arranged by size:
2149 341 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1334 1394 1534 2520
Size: 1334 60 140 986
Fragments arranged by size:
1334 986 140 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 295 2520
Size: 84 211 2225
Fragments arranged by size:
2225 211 84
BsiEI CG_ry'CG
Cuts at: 0 96 2520
Size: 96 2424
BsmI GAATG_Cn'
Cuts at: 0 220 1548 2520
Size: 220 1328 972
Fragments arranged by size:
1328 972 220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1742 2520
Size: 1742 778
BsrBI CCG'CTC
Cuts at: 0 1839 2520
Size: 1839 681
BsrDI GCAATG_nn'
Cuts at: 0 936 1129 2228 2520
Size: 936 193 1099 292
Fragments arranged by size:
1099 936 292 193
BsrFI r'CCGG_y
Cuts at: 0 58 105 2520
Size: 58 47 2415
Fragments arranged by size:
2415 58 47
BsrGI T'GTAC_A
Cuts at: 0 1787 2520
Size: 1787 733
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1728 2520
Size: 1728 792
BstYI r'GATC_y
Cuts at: 0 558 846 1365 1663 2114 2277 2520
Size: 558 288 519 298 451 163 243
Fragments arranged by size:
558 519 451 298 288 243 163
Bsu36I CC'TnA_GG
Cuts at: 0 1346 2520
Size: 1346 1174
BtgI C'CryG_G
Cuts at: 0 210 1458 2273 2520
Size: 210 1248 815 247
Fragments arranged by size:
1248 815 247 210
BtsI GCAGTG_nn'
Cuts at: 0 521 671 1016 1141 1865 2520
Size: 521 150 345 125 724 655
Fragments arranged by size:
724 655 521 345 150 125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 510 545 660 695 1878 1913 2520
Size: 510 35 115 35 1183 35 607
Fragments arranged by size:
1183 607 510 115 35 35 35
DraI TTT'AAA
Cuts at: 0 201 2520
Size: 201 2319
DrdI GACnn_nn'nnGTC
Cuts at: 0 1252 2520
Size: 1252 1268
EaeI y'GGCC_r
Cuts at: 0 213 668 2520
Size: 213 455 1852
Fragments arranged by size:
1852 455 213
EarI CTCTTCn'nnn_
Cuts at: 0 317 569 1550 1589 2520
Size: 317 252 981 39 931
Fragments arranged by size:
981 931 317 252 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 827 940 1208 1250 1404 1658 1849
Size: 394 433 113 268 42 154 254 191
Cuts at: 1849 2096 2520
Size: 247 424
Fragments arranged by size:
433 424 394 268 254 247 191 154
113 42
EcoO109I rG'GnC_Cy
Cuts at: 0 2440 2520
Size: 2440 80
EcoRI G'AATT_C
Cuts at: 0 1218 2520
Size: 1218 1302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 754 786 1439 1471 2262 2294
Size: 474 32 248 32 653 32 791 32
Cuts at: 2294 2520
Size: 226
Fragments arranged by size:
791 653 474 248 226 32 32 32
32
HincII GTy'rAC
Cuts at: 0 414 2047 2520
Size: 414 1633 473
Fragments arranged by size:
1633 473 414
HindIII A'AGCT_T
Cuts at: 0 1891 2497 2520
Size: 1891 606 23
Fragments arranged by size:
1891 606 23
MfeI C'AATT_G
Cuts at: 0 140 2520
Size: 140 2380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2142 2520
Size: 2142 378
MscI TGG'CCA
Cuts at: 0 670 2520
Size: 670 1850
MslI CAynn'nnrTG
Cuts at: 0 731 1134 2177 2473 2520
Size: 731 403 1043 296 47
Fragments arranged by size:
1043 731 403 296 47
MspA1I CmG'CkG
Cuts at: 0 98 1228 2473 2520
Size: 98 1130 1245 47
Fragments arranged by size:
1245 1130 98 47
NaeI GCC'GGC
Cuts at: 0 60 107 2520
Size: 60 47 2413
Fragments arranged by size:
2413 60 47
NcoI C'CATG_G
Cuts at: 0 210 1458 2273 2520
Size: 210 1248 815 247
Fragments arranged by size:
1248 815 247 210
NdeI CA'TA_TG
Cuts at: 0 264 540 2520
Size: 264 276 1980
Fragments arranged by size:
1980 276 264
NgoMIV G'CCGG_C
Cuts at: 0 58 105 2520
Size: 58 47 2415
Fragments arranged by size:
2415 58 47
NspI r_CATG'y
Cuts at: 0 1591 2520
Size: 1591 929
PciI A'CATG_T
Cuts at: 0 1587 2520
Size: 1587 933
PflMI CCAn_nnn'nTGG
Cuts at: 0 2419 2520
Size: 2419 101
PfoI T'CCnGG_A
Cuts at: 0 2066 2520
Size: 2066 454
PspXI vC'TCGA_Gb
Cuts at: 0 1118 2520
Size: 1118 1402
PstI C_TGCA'G
Cuts at: 0 528 2255 2520
Size: 528 1727 265
Fragments arranged by size:
1727 528 265
PvuII CAG'CTG
Cuts at: 0 1228 2473 2520
Size: 1228 1245 47
Fragments arranged by size:
1245 1228 47
SapI GCTCTTCn'nnn_
Cuts at: 0 317 1589 2520
Size: 317 1272 931
Fragments arranged by size:
1272 931 317
ScaI AGT'ACT
Cuts at: 0 1725 2331 2520
Size: 1725 606 189
Fragments arranged by size:
1725 606 189
SfcI C'TryA_G
Cuts at: 0 284 407 524 1335 1373 2251 2520
Size: 284 123 117 811 38 878 269
Fragments arranged by size:
878 811 284 269 123 117 38
SgrAI Cr'CCGG_yG
Cuts at: 0 58 2520
Size: 58 2462
SmlI C'TyrA_G
Cuts at: 0 397 817 1118 1324 1655 2520
Size: 397 420 301 206 331 865
Fragments arranged by size:
865 420 397 331 301 206
StuI AGG'CCT
Cuts at: 0 1330 2520
Size: 1330 1190
StyI C'CwwG_G
Cuts at: 0 192 210 1458 1667 2273 2520
Size: 192 18 1248 209 606 247
Fragments arranged by size:
1248 606 247 209 192 18
TatI w'GTAC_w
Cuts at: 0 1087 1723 1787 2329 2520
Size: 1087 636 64 542 191
Fragments arranged by size:
1087 636 542 191 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2364 2520
Size: 2364 156
XhoI C'TCGA_G
Cuts at: 0 1118 2520
Size: 1118 1402
XmnI GAAnn'nnTTC
Cuts at: 0 392 2520
Size: 392 2128
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI
ApoI AseI AvaI BamHI BbsI BcgI BclI BglI
BglII BlpI BplI BpmI BpuEI BsaI BsaXI BseRI
BsgI BsiEI BsmI BspMI BsrBI BsrDI BsrFI BsrGI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DrdI
EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PspXI PstI PvuII
SapI ScaI SfcI SgrAI SmlI StuI StyI TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BanI BanII BbeI
BbvCI BciVI BfrBI Bme1580I BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsaHI BsaWI BseYI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI DraIII EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NarI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XmaI
ZraI