(Linear) (Six-base) MAPSORT of: KIBB8474.seq Check: 6367 from: 1 to: 2523 KIBB8474.orf With 182 enzymes: SgfI * October 7, 2009 14:25 .. AccI GT'mk_AC Cuts at: 0 1197 1365 2523 Size: 1197 168 1158 Fragments arranged by size: 1197 1158 168 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 1693 1768 1786 1992 2523 Size: 370 1323 75 18 206 531 Fragments arranged by size: 1323 531 370 206 75 18 AhdI GACnn_n'nnGTC Cuts at: 0 130 2523 Size: 130 2393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1679 2523 Size: 1679 844 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 136 168 2523 Size: 136 32 2355 Fragments arranged by size: 2355 136 32 AlwNI CAG_nnn'CTG Cuts at: 0 51 348 840 2142 2523 Size: 51 297 492 1302 381 Fragments arranged by size: 1302 492 381 297 51 ApaI G_GGCC'C Cuts at: 0 392 535 936 2523 Size: 392 143 401 1587 Fragments arranged by size: 1587 401 392 143 AvaI C'yCGr_G Cuts at: 0 1714 2523 Size: 1714 809 BamHI G'GATC_C Cuts at: 0 519 1802 2523 Size: 519 1283 721 Fragments arranged by size: 1283 721 519 BanII G_rGCy'C Cuts at: 0 392 535 646 936 1418 1460 2044 2132 Size: 392 143 111 290 482 42 584 88 Cuts at: 2132 2523 Size: 391 Fragments arranged by size: 584 482 392 391 290 143 111 88 42 BbsI GAAGACnn'nnnn_ Cuts at: 0 112 1439 1756 2523 Size: 112 1327 317 767 Fragments arranged by size: 1327 767 317 112 BbvCI CC'TCA_GC Cuts at: 0 412 2523 Size: 412 2111 BciVI GTATCCnnnnn_n' Cuts at: 0 960 2523 Size: 960 1563 BclI T'GATC_A Cuts at: 0 807 2523 Size: 807 1716 BglI GCCn_nnn'nGGC Cuts at: 0 823 1928 2094 2523 Size: 823 1105 166 429 Fragments arranged by size: 1105 823 429 166 BglII A'GATC_T Cuts at: 0 2021 2523 Size: 2021 502 BlpI GC'TnA_GC Cuts at: 0 44 686 2126 2467 2523 Size: 44 642 1440 341 56 Fragments arranged by size: 1440 642 341 56 44 Bme1580I G_kGCm'C Cuts at: 0 392 535 936 1401 2523 Size: 392 143 401 465 1122 Fragments arranged by size: 1122 465 401 392 143 BmrI ACTGGGnnnn_n' Cuts at: 0 813 1100 2523 Size: 813 287 1423 Fragments arranged by size: 1423 813 287 BmtI G_CTAG'C Cuts at: 0 1309 2523 Size: 1309 1214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1977 2523 Size: 1977 546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 1054 1156 1495 1522 1828 2194 2416 Size: 349 705 102 339 27 306 366 222 Cuts at: 2416 2523 Size: 107 Fragments arranged by size: 705 366 349 339 306 222 107 102 27 Bpu10I CC'TnA_GC Cuts at: 0 194 412 1538 1926 2523 Size: 194 218 1126 388 597 Fragments arranged by size: 1126 597 388 218 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1032 1624 1816 1825 1954 2063 2523 Size: 874 158 592 192 9 129 109 460 Fragments arranged by size: 874 592 460 192 158 129 109 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 2245 2327 2523 Size: 2245 82 196 Fragments arranged by size: 2245 196 82 BsaWI w'CCGG_w Cuts at: 0 59 2523 Size: 59 2464 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 359 389 739 769 1147 1177 2523 Size: 359 30 350 30 378 30 1346 Fragments arranged by size: 1346 378 359 350 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 37 271 616 1465 1468 1492 1699 Size: 34 3 234 345 849 3 24 207 Cuts at: 1699 1702 1744 1951 1992 2191 2413 2523 Size: 3 42 207 41 199 222 110 Fragments arranged by size: 849 345 234 222 207 207 199 110 42 41 34 24 3 3 3 BseYI C'CCAG_C Cuts at: 0 385 1166 1334 1350 1386 2084 2316 2523 Size: 385 781 168 16 36 698 232 207 Fragments arranged by size: 781 698 385 232 207 168 36 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 983 2523 Size: 229 754 1540 Fragments arranged by size: 1540 754 229 BsiHKAI G_wGCw'C Cuts at: 0 646 1418 1460 2044 2132 2523 Size: 646 772 42 584 88 391 Fragments arranged by size: 772 646 584 391 88 42 Bsp1286I G_dGCh'C Cuts at: 0 392 535 646 936 1401 1418 1460 2044 Size: 392 143 111 290 465 17 42 584 Cuts at: 2044 2132 2523 Size: 88 391 Fragments arranged by size: 584 465 392 391 290 143 111 88 42 17 BspEI T'CCGG_A Cuts at: 0 59 2523 Size: 59 2464 BsrBI CCG'CTC Cuts at: 0 1734 1863 1925 2523 Size: 1734 129 62 598 Fragments arranged by size: 1734 598 129 62 BsrDI GCAATG_nn' Cuts at: 0 1316 2523 Size: 1316 1207 BssSI C'ACGA_G Cuts at: 0 394 2523 Size: 394 2129 BstAPI GCAn_nnn'nTGC Cuts at: 0 308 376 2391 2477 2523 Size: 308 68 2015 86 46 Fragments arranged by size: 2015 308 86 68 46 BstXI CCAn_nnnn'nTGG Cuts at: 0 549 564 1095 2523 Size: 549 15 531 1428 Fragments arranged by size: 1428 549 531 15 BstYI r'GATC_y Cuts at: 0 519 1040 1469 1802 2021 2523 Size: 519 521 429 333 219 502 Fragments arranged by size: 521 519 502 429 333 219 BtgI C'CryG_G Cuts at: 0 1461 1550 2213 2523 Size: 1461 89 663 310 Fragments arranged by size: 1461 663 310 89 BtsI GCAGTG_nn' Cuts at: 0 1651 1827 2211 2523 Size: 1651 176 384 312 Fragments arranged by size: 1651 384 312 176 ClaI AT'CG_AT Cuts at: 0 2276 2523 Size: 2276 247 EaeI y'GGCC_r Cuts at: 0 290 2095 2523 Size: 290 1805 428 Fragments arranged by size: 1805 428 290 EarI CTCTTCn'nnn_ Cuts at: 0 601 1264 1340 2230 2523 Size: 601 663 76 890 293 Fragments arranged by size: 890 663 601 293 76 EcoICRI GAG'CTC Cuts at: 0 644 1416 1458 2042 2130 2523 Size: 644 772 42 584 88 393 Fragments arranged by size: 772 644 584 393 88 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 370 1054 1156 1495 1522 1693 1768 Size: 349 21 684 102 339 27 171 75 Cuts at: 1768 1786 1828 1992 2194 2416 2523 Size: 18 42 164 202 222 107 Fragments arranged by size: 684 349 339 222 202 171 164 107 102 75 42 27 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 1768 2523 Size: 1768 755 EcoO109I rG'GnC_Cy Cuts at: 0 389 531 933 1134 1836 2060 2223 2485 Size: 389 142 402 201 702 224 163 262 Cuts at: 2485 2523 Size: 38 Fragments arranged by size: 702 402 389 262 224 201 163 142 38 HaeII r_GCGC'y Cuts at: 0 909 1584 2243 2523 Size: 909 675 659 280 Fragments arranged by size: 909 675 659 280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 59 116 148 934 966 1147 1179 Size: 27 32 57 32 786 32 181 32 Cuts at: 1179 2523 Size: 1344 Fragments arranged by size: 1344 786 181 57 32 32 32 32 27 HincII GTy'rAC Cuts at: 0 1198 1244 2523 Size: 1198 46 1279 Fragments arranged by size: 1279 1198 46 MslI CAynn'nnrTG Cuts at: 0 286 2523 Size: 286 2237 MspA1I CmG'CkG Cuts at: 0 33 327 499 627 894 1102 1851 2139 Size: 33 294 172 128 267 208 749 288 Cuts at: 2139 2312 2523 Size: 173 211 Fragments arranged by size: 749 294 288 267 211 208 173 172 128 33 NcoI C'CATG_G Cuts at: 0 1550 2213 2523 Size: 1550 663 310 Fragments arranged by size: 1550 663 310 NheI G'CTAG_C Cuts at: 0 1305 2523 Size: 1305 1218 NspI r_CATG'y Cuts at: 0 919 2394 2523 Size: 919 1475 129 Fragments arranged by size: 1475 919 129 PasI CC'CwG_GG Cuts at: 0 526 2523 Size: 526 1997 PflMI CCAn_nnn'nTGG Cuts at: 0 1568 2523 Size: 1568 955 PfoI T'CCnGG_A Cuts at: 0 50 1076 1172 1472 1805 2523 Size: 50 1026 96 300 333 718 Fragments arranged by size: 1026 718 333 300 96 50 PpuMI rG'GwC_Cy Cuts at: 0 1134 1836 2060 2223 2523 Size: 1134 702 224 163 300 Fragments arranged by size: 1134 702 300 224 163 PshAI GACnn'nnGTC Cuts at: 0 2257 2523 Size: 2257 266 PspOMI G'GGCC_C Cuts at: 0 388 531 932 2523 Size: 388 143 401 1591 Fragments arranged by size: 1591 401 388 143 PspXI vC'TCGA_Gb Cuts at: 0 1714 2523 Size: 1714 809 PstI C_TGCA'G Cuts at: 0 236 313 366 464 674 737 845 1610 Size: 236 77 53 98 210 63 108 765 Cuts at: 1610 1886 1898 2387 2523 Size: 276 12 489 136 Fragments arranged by size: 765 489 276 236 210 136 108 98 77 63 53 12 PvuII CAG'CTG Cuts at: 0 327 499 627 1102 1851 2523 Size: 327 172 128 475 749 672 Fragments arranged by size: 749 672 475 327 172 128 SacI G_AGCT'C Cuts at: 0 646 1418 1460 2044 2132 2523 Size: 646 772 42 584 88 391 Fragments arranged by size: 772 646 584 391 88 42 SalI G'TCGA_C Cuts at: 0 1196 2523 Size: 1196 1327 SapI GCTCTTCn'nnn_ Cuts at: 0 1340 2230 2523 Size: 1340 890 293 Fragments arranged by size: 1340 890 293 SbfI CC_TGCA'GG Cuts at: 0 464 2523 Size: 464 2059 SfcI C'TryA_G Cuts at: 0 232 309 362 418 460 670 733 841 Size: 232 77 53 56 42 210 63 108 Cuts at: 841 1606 1693 1882 1894 2383 2523 Size: 765 87 189 12 489 140 Fragments arranged by size: 765 489 232 210 189 140 108 87 77 63 56 53 42 12 SmlI C'TyrA_G Cuts at: 0 853 1011 1639 1714 1831 1840 1933 2078 Size: 853 158 628 75 117 9 93 145 Cuts at: 2078 2523 Size: 445 Fragments arranged by size: 853 628 445 158 145 117 93 75 9 StuI AGG'CCT Cuts at: 0 1931 2523 Size: 1931 592 StyI C'CwwG_G Cuts at: 0 1550 1562 2213 2523 Size: 1550 12 651 310 Fragments arranged by size: 1550 651 310 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 870 2523 Size: 870 1653 XhoI C'TCGA_G Cuts at: 0 1714 2523 Size: 1714 809 XmnI GAAnn'nnTTC Cuts at: 0 1211 1529 2523 Size: 1211 318 994 Fragments arranged by size: 1211 994 318 Enzymes that do cut: AccI AcuI AhdI AlfI AloI AlwNI ApaI AvaI BamHI BanII BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MslI MspA1I NcoI NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SalI SapI SbfI SfcI SmlI StuI StyI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BfrBI BmgBI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacII SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XmaI ZraI