Restriction map for
pF1KB8476
(Linear) (Six-base) MAPSORT of: KIBB8476.seq Check: 264 from: 1 to: 2538
KIBB8476.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 101 2538
Size: 101 2437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 932 986 1226 1607 1930 2538
Size: 625 307 54 240 381 323 608
Fragments arranged by size:
625 608 381 323 307 240 54
AleI CACnn'nnGTG
Cuts at: 0 1656 2538
Size: 1656 882
ApoI r'AATT_y
Cuts at: 0 1011 1092 1280 1825 1969 2233 2538
Size: 1011 81 188 545 144 264 305
Fragments arranged by size:
1011 545 305 264 188 144 81
AseI AT'TA_AT
Cuts at: 0 1526 2538
Size: 1526 1012
BanI G'GyrC_C
Cuts at: 0 85 529 2538
Size: 85 444 2009
Fragments arranged by size:
2009 444 85
BanII G_rGCy'C
Cuts at: 0 678 855 938 1275 2538
Size: 678 177 83 337 1263
Fragments arranged by size:
1263 678 337 177 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1214 1296 2538
Size: 1214 82 1242
Fragments arranged by size:
1242 1214 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 1538 2538
Size: 1538 1000
BfrBI ATG'CAT
Cuts at: 0 411 2538
Size: 411 2127
BglII A'GATC_T
Cuts at: 0 964 2538
Size: 964 1574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 2538
Size: 185 2353
Bpu10I CC'TnA_GC
Cuts at: 0 1181 2538
Size: 1181 1357
BsaI GGTCTCn'nnnn_
Cuts at: 0 847 2538
Size: 847 1691
BsaBI GATnn'nnATC
Cuts at: 0 1080 2538
Size: 1080 1458
BsaHI Gr'CG_yC
Cuts at: 0 98 2538
Size: 98 2440
BsaWI w'CCGG_w
Cuts at: 0 1218 2538
Size: 1218 1320
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1028 2538
Size: 1028 1510
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2180 2477 2538
Size: 2180 297 61
Fragments arranged by size:
2180 297 61
BsiHKAI G_wGCw'C
Cuts at: 0 1275 2538
Size: 1275 1263
BsmI GAATG_Cn'
Cuts at: 0 984 2538
Size: 984 1554
Bsp1286I G_dGCh'C
Cuts at: 0 678 855 938 1275 2538
Size: 678 177 83 337 1263
Fragments arranged by size:
1263 678 337 177 83
BspEI T'CCGG_A
Cuts at: 0 1218 2538
Size: 1218 1320
BsrDI GCAATG_nn'
Cuts at: 0 503 2538
Size: 503 2035
BsrGI T'GTAC_A
Cuts at: 0 117 2538
Size: 117 2421
BssSI C'ACGA_G
Cuts at: 0 1061 2538
Size: 1061 1477
BstEII G'GTnAC_C
Cuts at: 0 2397 2538
Size: 2397 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 325 2538
Size: 325 2213
BstYI r'GATC_y
Cuts at: 0 964 2538
Size: 964 1574
Bsu36I CC'TnA_GG
Cuts at: 0 34 2538
Size: 34 2504
BtgI C'CryG_G
Cuts at: 0 23 450 1651 2538
Size: 23 427 1201 887
Fragments arranged by size:
1201 887 427 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 470 2538
Size: 470 2068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1855 1890 2538
Size: 1855 35 648
Fragments arranged by size:
1855 648 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2455 2538
Size: 2455 83
EarI CTCTTCn'nnn_
Cuts at: 0 685 2538
Size: 685 1853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 54 311 2538
Size: 54 257 2227
Fragments arranged by size:
2227 257 54
EcoICRI GAG'CTC
Cuts at: 0 1273 2538
Size: 1273 1265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 625 932 986 1226 1607 1930 2538
Size: 185 440 307 54 240 381 323 608
Fragments arranged by size:
608 440 381 323 307 240 185 54
EcoO109I rG'GnC_Cy
Cuts at: 0 6 2538
Size: 6 2532
EcoRV GAT'ATC
Cuts at: 0 420 600 2538
Size: 420 180 1938
Fragments arranged by size:
1938 420 180
HindIII A'AGCT_T
Cuts at: 0 2379 2538
Size: 2379 159
MfeI C'AATT_G
Cuts at: 0 623 2538
Size: 623 1915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2180 2538
Size: 2180 358
MslI CAynn'nnrTG
Cuts at: 0 455 1656 2139 2538
Size: 455 1201 483 399
Fragments arranged by size:
1201 483 455 399
MspA1I CmG'CkG
Cuts at: 0 12 25 83 2538
Size: 12 13 58 2455
Fragments arranged by size:
2455 58 13 12
NcoI C'CATG_G
Cuts at: 0 450 2538
Size: 450 2088
NsiI A_TGCA'T
Cuts at: 0 413 2538
Size: 413 2125
NspI r_CATG'y
Cuts at: 0 1490 1759 2538
Size: 1490 269 779
Fragments arranged by size:
1490 779 269
PflMI CCAn_nnn'nTGG
Cuts at: 0 1309 2538
Size: 1309 1229
PfoI T'CCnGG_A
Cuts at: 0 1540 2538
Size: 1540 998
PstI C_TGCA'G
Cuts at: 0 141 2538
Size: 141 2397
SacI G_AGCT'C
Cuts at: 0 1275 2538
Size: 1275 1263
SacII CC_GC'GG
Cuts at: 0 26 2538
Size: 26 2512
SfcI C'TryA_G
Cuts at: 0 137 2321 2538
Size: 137 2184 217
Fragments arranged by size:
2184 217 137
SphI G_CATG'C
Cuts at: 0 1490 2538
Size: 1490 1048
SspI AAT'ATT
Cuts at: 0 197 435 2538
Size: 197 238 2103
Fragments arranged by size:
2103 238 197
StyI C'CwwG_G
Cuts at: 0 450 1186 2538
Size: 450 736 1352
Fragments arranged by size:
1352 736 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 680 1476 1823 2538
Size: 680 796 347 715
Fragments arranged by size:
796 715 680 347
TatI w'GTAC_w
Cuts at: 0 117 1568 1660 2538
Size: 117 1451 92 878
Fragments arranged by size:
1451 878 117 92
XbaI T'CTAG_A
Cuts at: 0 847 2538
Size: 847 1691
XmnI GAAnn'nnTTC
Cuts at: 0 809 2538
Size: 809 1729
ZraI GAC'GTC
Cuts at: 0 99 2538
Size: 99 2439
Enzymes that do cut:
AatII AcuI AleI ApoI AseI BanI BanII BbsI
BciVI BfrBI BglII BpmI Bpu10I BsaI BsaBI BsaHI
BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
CspCI DrdI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
HindIII MfeI MmeI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PstI SacI SacII SfcI SphI SspI
StyI TaqII TatI XbaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaXI BseYI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstBI BstZ17I BtsI ClaI
DraI DraIII EaeI EagI EcoNI EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
SwaI TstI Tth111I XcmI XhoI XmaI