Restriction map for
pF1KB8481
(Linear) (Six-base) MAPSORT of: KIBB8481.seq Check: 8294 from: 1 to: 2748
KIBB8481.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 1270 2686 2748
Size: 1270 1416 62
Fragments arranged by size:
1416 1270 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 213 805 829 1523 1574 1739 1745
Size: 54 159 592 24 694 51 165 6
Cuts at: 1745 2748
Size: 1003
Fragments arranged by size:
1003 694 592 165 159 54 51 24
6
AfeI AGC'GCT
Cuts at: 0 562 2748
Size: 562 2186
AflII C'TTAA_G
Cuts at: 0 1336 2748
Size: 1336 1412
AflIII A'CryG_T
Cuts at: 0 8 443 2748
Size: 8 435 2305
Fragments arranged by size:
2305 435 8
AlwNI CAG_nnn'CTG
Cuts at: 0 321 405 678 1095 1717 2748
Size: 321 84 273 417 622 1031
Fragments arranged by size:
1031 622 417 321 273 84
ApaLI G'TGCA_C
Cuts at: 0 355 2263 2748
Size: 355 1908 485
Fragments arranged by size:
1908 485 355
ApoI r'AATT_y
Cuts at: 0 1351 2748
Size: 1351 1397
AvaI C'yCGr_G
Cuts at: 0 63 2748
Size: 63 2685
BamHI G'GATC_C
Cuts at: 0 1654 2380 2748
Size: 1654 726 368
Fragments arranged by size:
1654 726 368
BanI G'GyrC_C
Cuts at: 0 962 1006 2371 2748
Size: 962 44 1365 377
Fragments arranged by size:
1365 962 377 44
BanII G_rGCy'C
Cuts at: 0 2512 2748
Size: 2512 236
BbeI G_GCGC'C
Cuts at: 0 966 2748
Size: 966 1782
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 793 2399 2748
Size: 545 248 1606 349
Fragments arranged by size:
1606 545 349 248
BbvCI CC'TCA_GC
Cuts at: 0 1066 2748
Size: 1066 1682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 316 350 609 643 2748
Size: 150 34 132 34 259 34 2105
Fragments arranged by size:
2105 259 150 132 34 34 34
BlpI GC'TnA_GC
Cuts at: 0 202 1033 2748
Size: 202 831 1715
Fragments arranged by size:
1715 831 202
Bme1580I G_kGCm'C
Cuts at: 0 359 1117 1892 1931 2267 2748
Size: 359 758 775 39 336 481
Fragments arranged by size:
775 758 481 359 336 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1268 2748
Size: 1268 1480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 2748
Size: 78 2670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 502 553 1042 1234 1517 2748
Size: 385 117 51 489 192 283 1231
Fragments arranged by size:
1231 489 385 283 192 117 51
Bpu10I CC'TnA_GC
Cuts at: 0 506 1066 2748
Size: 506 560 1682
Fragments arranged by size:
1682 560 506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 2526 2748
Size: 538 1988 222
Fragments arranged by size:
1988 538 222
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 2272 2437 2656 2748
Size: 49 2223 165 219 92
Fragments arranged by size:
2223 219 165 92 49
BsaHI Gr'CG_yC
Cuts at: 0 637 963 2748
Size: 637 326 1785
Fragments arranged by size:
1785 637 326
BsaWI w'CCGG_w
Cuts at: 0 262 2748
Size: 262 2486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 565 595 2748
Size: 244 30 291 30 2153
Fragments arranged by size:
2153 291 244 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 583 586 646 730 733 1060 1150
Size: 138 445 3 60 84 3 327 90
Cuts at: 1150 1375 1621 1810 2348 2365 2443 2488 2560
Size: 225 246 189 538 17 78 45 72
Cuts at: 2560 2686 2748
Size: 126 62
Fragments arranged by size:
538 445 327 246 225 189 138 126
90 84 78 72 62 60 45 17
3 3
BseYI C'CCAG_C
Cuts at: 0 21 133 431 435 824 2511 2748
Size: 21 112 298 4 389 1687 237
Fragments arranged by size:
1687 389 298 237 112 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 691 1532 2748
Size: 532 159 841 1216
Fragments arranged by size:
1216 841 532 159
BsiEI CG_ry'CG
Cuts at: 0 330 908 2748
Size: 330 578 1840
Fragments arranged by size:
1840 578 330
BsiHKAI G_wGCw'C
Cuts at: 0 359 2267 2748
Size: 359 1908 481
Fragments arranged by size:
1908 481 359
BsiWI C'GTAC_G
Cuts at: 0 446 2748
Size: 446 2302
Bsp1286I G_dGCh'C
Cuts at: 0 359 1117 1892 1931 2267 2512 2748
Size: 359 758 775 39 336 245 236
Fragments arranged by size:
775 758 359 336 245 236 39
BspEI T'CCGG_A
Cuts at: 0 262 2748
Size: 262 2486
BsrBI CCG'CTC
Cuts at: 0 62 107 293 821 932 1026 2748
Size: 62 45 186 528 111 94 1722
Fragments arranged by size:
1722 528 186 111 94 62 45
BsrFI r'CCGG_y
Cuts at: 0 41 337 548 959 2748
Size: 41 296 211 411 1789
Fragments arranged by size:
1789 411 296 211 41
BssHII G'CGCG_C
Cuts at: 0 471 611 2748
Size: 471 140 2137
Fragments arranged by size:
2137 471 140
BssSI C'ACGA_G
Cuts at: 0 496 712 2748
Size: 496 216 2036
Fragments arranged by size:
2036 496 216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 678 2748
Size: 678 2070
BstEII G'GTnAC_C
Cuts at: 0 48 2238 2559 2748
Size: 48 2190 321 189
Fragments arranged by size:
2190 321 189 48
BstYI r'GATC_y
Cuts at: 0 395 458 743 1654 2380 2748
Size: 395 63 285 911 726 368
Fragments arranged by size:
911 726 395 368 285 63
BstZ17I GTA'TAC
Cuts at: 0 1271 2748
Size: 1271 1477
BtgI C'CryG_G
Cuts at: 0 123 2748
Size: 123 2625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 2748
Size: 737 2011
BtsI GCAGTG_nn'
Cuts at: 0 2299 2466 2748
Size: 2299 167 282
Fragments arranged by size:
2299 282 167
DraIII CAC_nnn'GTG
Cuts at: 0 1121 2748
Size: 1121 1627
DrdI GACnn_nn'nnGTC
Cuts at: 0 2401 2748
Size: 2401 347
EaeI y'GGCC_r
Cuts at: 0 194 602 725 905 1439 2748
Size: 194 408 123 180 534 1309
Fragments arranged by size:
1309 534 408 194 180 123
EagI C'GGCC_G
Cuts at: 0 905 2748
Size: 905 1843
EarI CTCTTCn'nnn_
Cuts at: 0 860 938 1217 1403 1451 1481 1559 1598
Size: 860 78 279 186 48 30 78 39
Cuts at: 1598 1640 1862 1901 1940 1979 2018 2057 2138
Size: 42 222 39 39 39 39 39 81
Cuts at: 2138 2159 2282 2465 2645 2654 2748
Size: 21 123 183 180 9 94
Fragments arranged by size:
860 279 222 186 183 180 123 94
81 78 78 48 42 39 39 39
39 39 39 30 21 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 198 406 924 2748
Size: 24 174 208 518 1824
Fragments arranged by size:
1824 518 208 174 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 213 385 502 553 805 829 1042
Size: 54 159 172 117 51 252 24 213
Cuts at: 1042 1234 1517 1523 1574 1739 1745 2748
Size: 192 283 6 51 165 6 1003
Fragments arranged by size:
1003 283 252 213 192 172 165 159
117 54 51 51 24 6 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1155 1413 2748
Size: 1155 258 1335
Fragments arranged by size:
1335 1155 258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 579 2283 2748
Size: 579 1704 465
Fragments arranged by size:
1704 579 465
FspI TGC'GCA
Cuts at: 0 619 2748
Size: 619 2129
HaeII r_GCGC'y
Cuts at: 0 406 564 808 966 1053 2748
Size: 406 158 244 158 87 1695
Fragments arranged by size:
1695 406 244 158 158 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 979 1011 1271 1303 1381 1413
Size: 622 32 325 32 260 32 78 32
Cuts at: 1413 1639 1671 2056 2088 2748
Size: 226 32 385 32 660
Fragments arranged by size:
660 622 385 325 260 226 78 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1333 2748
Size: 1333 1415
KasI G'GCGC_C
Cuts at: 0 962 2748
Size: 962 1786
MluI A'CGCG_T
Cuts at: 0 443 2748
Size: 443 2305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1368 2748
Size: 320 1048 1380
Fragments arranged by size:
1380 1048 320
MslI CAynn'nnrTG
Cuts at: 0 763 2748
Size: 763 1985
MspA1I CmG'CkG
Cuts at: 0 83 125 312 435 516 969 1095 2748
Size: 83 42 187 123 81 453 126 1653
Fragments arranged by size:
1653 453 187 126 123 83 81 42
NaeI GCC'GGC
Cuts at: 0 339 2748
Size: 339 2409
NarI GG'CG_CC
Cuts at: 0 963 2748
Size: 963 1785
NgoMIV G'CCGG_C
Cuts at: 0 337 2748
Size: 337 2411
NspI r_CATG'y
Cuts at: 0 168 2748
Size: 168 2580
PflMI CCAn_nnn'nTGG
Cuts at: 0 1095 2748
Size: 1095 1653
PfoI T'CCnGG_A
Cuts at: 0 1211 2383 2748
Size: 1211 1172 365
Fragments arranged by size:
1211 1172 365
PpuMI rG'GwC_Cy
Cuts at: 0 1155 2748
Size: 1155 1593
PspXI vC'TCGA_Gb
Cuts at: 0 63 2748
Size: 63 2685
PstI C_TGCA'G
Cuts at: 0 317 683 974 2306 2748
Size: 317 366 291 1332 442
Fragments arranged by size:
1332 442 366 317 291
PvuII CAG'CTG
Cuts at: 0 312 435 516 969 1095 2748
Size: 312 123 81 453 126 1653
Fragments arranged by size:
1653 453 312 126 123 81
SacII CC_GC'GG
Cuts at: 0 126 2748
Size: 126 2622
SapI GCTCTTCn'nnn_
Cuts at: 0 2645 2748
Size: 2645 103
SfcI C'TryA_G
Cuts at: 0 313 679 970 2302 2748
Size: 313 366 291 1332 446
Fragments arranged by size:
1332 446 366 313 291
SfoI GGC'GCC
Cuts at: 0 964 2748
Size: 964 1784
SmlI C'TyrA_G
Cuts at: 0 63 553 1336 2505 2748
Size: 63 490 783 1169 243
Fragments arranged by size:
1169 783 490 243 63
SpeI A'CTAG_T
Cuts at: 0 2679 2748
Size: 2679 69
SphI G_CATG'C
Cuts at: 0 168 2748
Size: 168 2580
StuI AGG'CCT
Cuts at: 0 421 2748
Size: 421 2327
StyI C'CwwG_G
Cuts at: 0 938 1010 1316 1790 1814 2138 2375 2447
Size: 938 72 306 474 24 324 237 72
Cuts at: 2447 2748
Size: 301
Fragments arranged by size:
938 474 324 306 301 237 72 72
24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 1017 1092 1797 1899 1938 2748
Size: 483 534 75 705 102 39 810
Fragments arranged by size:
810 705 534 483 102 75 39
XhoI C'TCGA_G
Cuts at: 0 63 2748
Size: 63 2685
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstEII BstYI BstZ17I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HindIII KasI MluI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PpuMI PspXI
PstI PvuII SacII SapI SfcI SfoI SmlI SpeI
SphI StuI StyI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BsaAI BsaBI
BsmI BsmBI BspHI BspMI BsrDI BsrGI BstBI BstXI
Bsu36I ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HincII HpaI KpnI MfeI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XmaI XmnI ZraI