Restriction map for
pF1KB8485
(Linear) (Six-base) MAPSORT of: KIBB8485.seq Check: 2377 from: 1 to: 2769
KIBB8485.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 728 2769
Size: 728 2041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 477 813 2329 2769
Size: 2 475 336 1516 440
Fragments arranged by size:
1516 475 440 336 2
AflIII A'CryG_T
Cuts at: 0 743 1050 1659 2769
Size: 743 307 609 1110
Fragments arranged by size:
1110 743 609 307
AhdI GACnn_n'nnGTC
Cuts at: 0 888 2211 2769
Size: 888 1323 558
Fragments arranged by size:
1323 888 558
AleI CACnn'nnGTG
Cuts at: 0 1160 1672 2769
Size: 1160 512 1097
Fragments arranged by size:
1160 1097 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1178 1333 1914 2769
Size: 1178 155 581 855
Fragments arranged by size:
1178 855 581 155
AlwNI CAG_nnn'CTG
Cuts at: 0 183 480 603 1307 1788 2587 2769
Size: 183 297 123 704 481 799 182
Fragments arranged by size:
799 704 481 297 183 182 123
ApaI G_GGCC'C
Cuts at: 0 77 2769
Size: 77 2692
ApaLI G'TGCA_C
Cuts at: 0 1173 2769
Size: 1173 1596
AvaI C'yCGr_G
Cuts at: 0 70 1627 1705 2769
Size: 70 1557 78 1064
Fragments arranged by size:
1557 1064 78 70
BanI G'GyrC_C
Cuts at: 0 673 736 1295 1439 2059 2146 2769
Size: 673 63 559 144 620 87 623
Fragments arranged by size:
673 623 620 559 144 87 63
BanII G_rGCy'C
Cuts at: 0 77 359 826 1126 1535 2087 2769
Size: 77 282 467 300 409 552 682
Fragments arranged by size:
682 552 467 409 300 282 77
BbeI G_GCGC'C
Cuts at: 0 1443 2150 2769
Size: 1443 707 619
Fragments arranged by size:
1443 707 619
BbsI GAAGACnn'nnnn_
Cuts at: 0 8 794 2769
Size: 8 786 1975
Fragments arranged by size:
1975 786 8
BbvCI CC'TCA_GC
Cuts at: 0 184 2769
Size: 184 2585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 665 2743 2769
Size: 631 34 2078 26
Fragments arranged by size:
2078 631 34 26
BclI T'GATC_A
Cuts at: 0 1482 2249 2769
Size: 1482 767 520
Fragments arranged by size:
1482 767 520
BglI GCCn_nnn'nGGC
Cuts at: 0 298 1207 1426 2769
Size: 298 909 219 1343
Fragments arranged by size:
1343 909 298 219
BglII A'GATC_T
Cuts at: 0 1691 2769
Size: 1691 1078
BlpI GC'TnA_GC
Cuts at: 0 1024 2179 2769
Size: 1024 1155 590
Fragments arranged by size:
1155 1024 590
Bme1580I G_kGCm'C
Cuts at: 0 77 135 676 739 1177 1636 2769
Size: 77 58 541 63 438 459 1133
Fragments arranged by size:
1133 541 459 438 77 63 58
BmgBI CAC'GTC
Cuts at: 0 645 1158 2769
Size: 645 513 1611
Fragments arranged by size:
1611 645 513
BmrI ACTGGGnnnn_n'
Cuts at: 0 1088 1819 2769
Size: 1088 731 950
Fragments arranged by size:
1088 950 731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 840 1089 1566 1713 2769
Size: 840 249 477 147 1056
Fragments arranged by size:
1056 840 477 249 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1213 1240 1468 2616 2769
Size: 301 912 27 228 1148 153
Fragments arranged by size:
1148 912 301 228 153 27
Bpu10I CC'TnA_GC
Cuts at: 0 184 1120 1789 2769
Size: 184 936 669 980
Fragments arranged by size:
980 936 669 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 2769
Size: 1447 1322
BsaAI yAC'GTr
Cuts at: 0 234 1053 1660 2769
Size: 234 819 607 1109
Fragments arranged by size:
1109 819 607 234
BsaHI Gr'CG_yC
Cuts at: 0 1440 2147 2212 2462 2769
Size: 1440 707 65 250 307
Fragments arranged by size:
1440 707 307 250 65
BsaWI w'CCGG_w
Cuts at: 0 215 1273 1469 2087 2769
Size: 215 1058 196 618 682
Fragments arranged by size:
1058 682 618 215 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 1238 1268 1298 2769
Size: 257 30 951 30 30 1471
Fragments arranged by size:
1471 951 257 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 772 1465 1539 2095 2769
Size: 73 699 693 74 556 674
Fragments arranged by size:
699 693 674 556 74 73
BseYI C'CCAG_C
Cuts at: 0 285 396 846 1187 2510 2769
Size: 285 111 450 341 1323 259
Fragments arranged by size:
1323 450 341 285 259 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 588 990 1171 1696 2602 2769
Size: 118 470 402 181 525 906 167
Fragments arranged by size:
906 525 470 402 181 167 118
BsiEI CG_ry'CG
Cuts at: 0 1169 2769
Size: 1169 1600
BsiHKAI G_wGCw'C
Cuts at: 0 538 909 1177 2087 2769
Size: 538 371 268 910 682
Fragments arranged by size:
910 682 538 371 268
BsmI GAATG_Cn'
Cuts at: 0 1354 2769
Size: 1354 1415
Bsp1286I G_dGCh'C
Cuts at: 0 77 135 359 538 676 739 826 909
Size: 77 58 224 179 138 63 87 83
Cuts at: 909 1126 1177 1535 1636 2087 2769
Size: 217 51 358 101 451 682
Fragments arranged by size:
682 451 358 224 217 179 138 101
87 83 77 63 58 51
BspEI T'CCGG_A
Cuts at: 0 215 2087 2769
Size: 215 1872 682
Fragments arranged by size:
1872 682 215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2403 2769
Size: 2403 366
BsrBI CCG'CTC
Cuts at: 0 1293 1793 2769
Size: 1293 500 976
Fragments arranged by size:
1293 976 500
BsrFI r'CCGG_y
Cuts at: 0 1436 2056 2769
Size: 1436 620 713
Fragments arranged by size:
1436 713 620
BssSI C'ACGA_G
Cuts at: 0 2762 2769
Size: 2762 7
BstEII G'GTnAC_C
Cuts at: 0 2727 2769
Size: 2727 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 1726 2518 2640 2769
Size: 162 1564 792 122 129
Fragments arranged by size:
1564 792 162 129 122
BstYI r'GATC_y
Cuts at: 0 146 1691 2333 2433 2549 2769
Size: 146 1545 642 100 116 220
Fragments arranged by size:
1545 642 220 146 116 100
BtgI C'CryG_G
Cuts at: 0 170 1412 1520 2537 2769
Size: 170 1242 108 1017 232
Fragments arranged by size:
1242 1017 232 170 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 848 1367 1414 2769
Size: 848 519 47 1355
Fragments arranged by size:
1355 848 519 47
BtsI GCAGTG_nn'
Cuts at: 0 304 432 576 782 1961 2588 2769
Size: 304 128 144 206 1179 627 181
Fragments arranged by size:
1179 627 304 206 181 144 128
DraIII CAC_nnn'GTG
Cuts at: 0 310 1752 2769
Size: 310 1442 1017
Fragments arranged by size:
1442 1017 310
DrdI GACnn_nn'nnGTC
Cuts at: 0 2004 2769
Size: 2004 765
EaeI y'GGCC_r
Cuts at: 0 405 1166 1208 1268 1386 1523 2171 2645
Size: 405 761 42 60 118 137 648 474
Cuts at: 2645 2769
Size: 124
Fragments arranged by size:
761 648 474 405 137 124 118 60
42
EagI C'GGCC_G
Cuts at: 0 1166 2769
Size: 1166 1603
EarI CTCTTCn'nnn_
Cuts at: 0 2323 2567 2769
Size: 2323 244 202
Fragments arranged by size:
2323 244 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2269 2769
Size: 2269 500
EcoICRI GAG'CTC
Cuts at: 0 2085 2769
Size: 2085 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 301 477 813 1213 1240 1468 2329
Size: 2 299 176 336 400 27 228 861
Cuts at: 2329 2616 2769
Size: 287 153
Fragments arranged by size:
861 400 336 299 287 228 176 153
27 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1447 1852 2410 2769
Size: 1447 405 558 359
Fragments arranged by size:
1447 558 405 359
EcoO109I rG'GnC_Cy
Cuts at: 0 74 299 629 990 1068 1785 2022 2565
Size: 74 225 330 361 78 717 237 543
Cuts at: 2565 2769
Size: 204
Fragments arranged by size:
717 543 361 330 237 225 204 78
74
FspI TGC'GCA
Cuts at: 0 458 2753 2769
Size: 458 2295 16
Fragments arranged by size:
2295 458 16
HaeII r_GCGC'y
Cuts at: 0 184 847 1443 2150 2343 2769
Size: 184 663 596 707 193 426
Fragments arranged by size:
707 663 596 426 193 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 2149 2181 2197 2229 2689 2721
Size: 874 32 1243 32 16 32 460 32
Cuts at: 2721 2769
Size: 48
Fragments arranged by size:
1243 874 460 48 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 2759 2769
Size: 2759 10
KasI G'GCGC_C
Cuts at: 0 1439 2146 2769
Size: 1439 707 623
Fragments arranged by size:
1439 707 623
MluI A'CGCG_T
Cuts at: 0 743 2769
Size: 743 2026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2031 2769
Size: 2031 738
MscI TGG'CCA
Cuts at: 0 407 1270 1525 2173 2769
Size: 407 863 255 648 596
Fragments arranged by size:
863 648 596 407 255
MslI CAynn'nnrTG
Cuts at: 0 643 679 1160 1672 2134 2769
Size: 643 36 481 512 462 635
Fragments arranged by size:
643 635 512 481 462 36
MspA1I CmG'CkG
Cuts at: 0 600 1213 2255 2734 2769
Size: 600 613 1042 479 35
Fragments arranged by size:
1042 613 600 479 35
NarI GG'CG_CC
Cuts at: 0 1440 2147 2769
Size: 1440 707 622
Fragments arranged by size:
1440 707 622
NcoI C'CATG_G
Cuts at: 0 170 1520 2769
Size: 170 1350 1249
Fragments arranged by size:
1350 1249 170
NspI r_CATG'y
Cuts at: 0 772 1563 2127 2262 2769
Size: 772 791 564 135 507
Fragments arranged by size:
791 772 564 507 135
PasI CC'CwG_GG
Cuts at: 0 1126 1719 2769
Size: 1126 593 1050
Fragments arranged by size:
1126 1050 593
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 879 1752 2769
Size: 591 288 873 1017
Fragments arranged by size:
1017 873 591 288
PfoI T'CCnGG_A
Cuts at: 0 449 547 1043 2552 2769
Size: 449 98 496 1509 217
Fragments arranged by size:
1509 496 449 217 98
PmlI CAC'GTG
Cuts at: 0 1053 2769
Size: 1053 1716
PpuMI rG'GwC_Cy
Cuts at: 0 990 1068 2022 2769
Size: 990 78 954 747
Fragments arranged by size:
990 954 747 78
PshAI GACnn'nnGTC
Cuts at: 0 2764 2769
Size: 2764 5
PspOMI G'GGCC_C
Cuts at: 0 73 2769
Size: 73 2696
PstI C_TGCA'G
Cuts at: 0 113 485 1109 1312 1322 2693 2769
Size: 113 372 624 203 10 1371 76
Fragments arranged by size:
1371 624 372 203 113 76 10
PvuII CAG'CTG
Cuts at: 0 600 2769
Size: 600 2169
RsrII CG'GwC_CG
Cuts at: 0 213 2769
Size: 213 2556
SacI G_AGCT'C
Cuts at: 0 2087 2769
Size: 2087 682
SapI GCTCTTCn'nnn_
Cuts at: 0 2567 2769
Size: 2567 202
SbfI CC_TGCA'GG
Cuts at: 0 1109 1322 2769
Size: 1109 213 1447
Fragments arranged by size:
1447 1109 213
SfcI C'TryA_G
Cuts at: 0 109 139 481 1105 1308 1318 2689 2769
Size: 109 30 342 624 203 10 1371 80
Fragments arranged by size:
1371 624 342 203 109 80 30 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 2769
Size: 298 2471
SfoI GGC'GCC
Cuts at: 0 1441 2148 2769
Size: 1441 707 621
Fragments arranged by size:
1441 707 621
SgrAI Cr'CCGG_yG
Cuts at: 0 1436 2769
Size: 1436 1333
SmaI CCC'GGG
Cuts at: 0 72 2769
Size: 72 2697
SphI G_CATG'C
Cuts at: 0 1563 2769
Size: 1563 1206
SrfI GCCC'GGGC
Cuts at: 0 72 2769
Size: 72 2697
StuI AGG'CCT
Cuts at: 0 935 1546 2042 2769
Size: 935 611 496 727
Fragments arranged by size:
935 727 611 496
StyI C'CwwG_G
Cuts at: 0 170 1029 1520 1526 1877 2307 2769
Size: 170 859 491 6 351 430 462
Fragments arranged by size:
859 491 462 430 351 170 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 388 934 2769
Size: 309 79 546 1835
Fragments arranged by size:
1835 546 309 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 1268 1300 2769
Size: 257 32 979 32 1469
Fragments arranged by size:
1469 979 257 32 32
XmaI C'CCGG_G
Cuts at: 0 70 2769
Size: 70 2699
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII RsrII SacI SapI SbfI SfcI SfiI SfoI
SgrAI SmaI SphI SrfI StuI StyI TaqII TstI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BfrBI BmtI BpuEI BsaBI BsiWI BsmBI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI
PvuI SacII SalI SanDI ScaI SexAI SgfI SmlI
SnaBI SpeI SspI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI