(Linear) (Six-base) MAPSORT of: KIBB8491.seq Check: 6937 from: 1 to: 2709 KIBB8491.orf With 182 enzymes: SgfI * October 7, 2009 14:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1536 2709 Size: 1536 1173 AccI GT'mk_AC Cuts at: 0 2186 2709 Size: 2186 523 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 1846 2512 2709 Size: 116 1730 666 197 Fragments arranged by size: 1730 666 197 116 AflII C'TTAA_G Cuts at: 0 1105 2709 Size: 1105 1604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 908 2709 Size: 908 1801 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2539 2571 2709 Size: 2539 32 138 Fragments arranged by size: 2539 138 32 AlwNI CAG_nnn'CTG Cuts at: 0 555 618 1125 1461 1485 1623 1959 2148 Size: 555 63 507 336 24 138 336 189 Cuts at: 2148 2709 Size: 561 Fragments arranged by size: 561 555 507 336 336 189 138 63 24 ApaI G_GGCC'C Cuts at: 0 1089 1927 2709 Size: 1089 838 782 Fragments arranged by size: 1089 838 782 ApoI r'AATT_y Cuts at: 0 2130 2709 Size: 2130 579 AvaI C'yCGr_G Cuts at: 0 34 155 626 1010 1577 1759 2447 2673 Size: 34 121 471 384 567 182 688 226 Cuts at: 2673 2709 Size: 36 Fragments arranged by size: 688 567 471 384 226 182 121 36 34 BamHI G'GATC_C Cuts at: 0 357 1331 2709 Size: 357 974 1378 Fragments arranged by size: 1378 974 357 BanI G'GyrC_C Cuts at: 0 2663 2709 Size: 2663 46 BanII G_rGCy'C Cuts at: 0 80 162 806 1089 1568 1927 1967 2463 Size: 80 82 644 283 479 359 40 496 Cuts at: 2463 2709 Size: 246 Fragments arranged by size: 644 496 479 359 283 246 82 80 40 BbvCI CC'TCA_GC Cuts at: 0 130 1553 2471 2709 Size: 130 1423 918 238 Fragments arranged by size: 1423 918 238 130 BciVI GTATCCnnnnn_n' Cuts at: 0 1251 2709 Size: 1251 1458 BfrBI ATG'CAT Cuts at: 0 565 2709 Size: 565 2144 BglI GCCn_nnn'nGGC Cuts at: 0 868 2709 Size: 868 1841 BglII A'GATC_T Cuts at: 0 1905 2709 Size: 1905 804 BlpI GC'TnA_GC Cuts at: 0 471 752 1702 2111 2709 Size: 471 281 950 409 598 Fragments arranged by size: 950 598 471 409 281 Bme1580I G_kGCm'C Cuts at: 0 1089 1927 2162 2666 2709 Size: 1089 838 235 504 43 Fragments arranged by size: 1089 838 504 235 43 BmrI ACTGGGnnnn_n' Cuts at: 0 17 1689 2709 Size: 17 1672 1020 Fragments arranged by size: 1672 1020 17 BmtI G_CTAG'C Cuts at: 0 1501 2709 Size: 1501 1208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1160 2346 2607 2709 Size: 1160 1186 261 102 Fragments arranged by size: 1186 1160 261 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 505 946 1360 1384 1645 1737 1892 Size: 301 204 441 414 24 261 92 155 Cuts at: 1892 1981 2137 2170 2709 Size: 89 156 33 539 Fragments arranged by size: 539 441 414 301 261 204 156 155 92 89 33 24 Bpu10I CC'TnA_GC Cuts at: 0 130 1397 1553 2289 2321 2471 2709 Size: 130 1267 156 736 32 150 238 Fragments arranged by size: 1267 736 238 156 150 130 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1715 2110 2228 2709 Size: 1715 395 118 481 Fragments arranged by size: 1715 481 395 118 BsaI GGTCTCn'nnnn_ Cuts at: 0 1192 2709 Size: 1192 1517 BsaAI yAC'GTr Cuts at: 0 340 2254 2709 Size: 340 1914 455 Fragments arranged by size: 1914 455 340 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 478 508 1791 1821 2539 2569 2709 Size: 478 30 1283 30 718 30 140 Fragments arranged by size: 1283 718 478 140 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 502 943 1147 1642 1727 1798 1803 1890 Size: 502 441 204 495 85 71 5 87 Cuts at: 1890 2322 2700 2709 Size: 432 378 9 Fragments arranged by size: 502 495 441 432 378 204 87 85 71 9 5 BseYI C'CCAG_C Cuts at: 0 107 161 1015 1088 1202 1993 2356 2453 Size: 107 54 854 73 114 791 363 97 Cuts at: 2453 2709 Size: 256 Fragments arranged by size: 854 791 363 256 114 107 97 73 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1243 1327 1378 1991 2709 Size: 1243 84 51 613 718 Fragments arranged by size: 1243 718 613 84 51 BsiEI CG_ry'CG Cuts at: 0 675 2709 Size: 675 2034 BsiHKAI G_wGCw'C Cuts at: 0 806 1100 1568 2709 Size: 806 294 468 1141 Fragments arranged by size: 1141 806 468 294 BsmI GAATG_Cn' Cuts at: 0 1944 2709 Size: 1944 765 BsmBI CGTCTCn'nnnn_ Cuts at: 0 381 2709 Size: 381 2328 Bsp1286I G_dGCh'C Cuts at: 0 80 162 806 1089 1100 1568 1927 1967 Size: 80 82 644 283 11 468 359 40 Cuts at: 1967 2162 2463 2666 2709 Size: 195 301 203 43 Fragments arranged by size: 644 468 359 301 283 203 195 82 80 43 40 11 BspHI T'CATG_A Cuts at: 0 192 2709 Size: 192 2517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1536 2709 Size: 1536 1173 BsrBI CCG'CTC Cuts at: 0 756 999 1383 2709 Size: 756 243 384 1326 Fragments arranged by size: 1326 756 384 243 BsrFI r'CCGG_y Cuts at: 0 134 740 2709 Size: 134 606 1969 Fragments arranged by size: 1969 606 134 BstAPI GCAn_nnn'nTGC Cuts at: 0 1665 2100 2145 2709 Size: 1665 435 45 564 Fragments arranged by size: 1665 564 435 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 1818 2709 Size: 1818 891 BstYI r'GATC_y Cuts at: 0 45 357 1331 1905 2061 2475 2570 2709 Size: 45 312 974 574 156 414 95 139 Fragments arranged by size: 974 574 414 312 156 139 95 45 Bsu36I CC'TnA_GG Cuts at: 0 1429 1766 2054 2709 Size: 1429 337 288 655 Fragments arranged by size: 1429 655 337 288 BtgI C'CryG_G Cuts at: 0 149 656 863 1811 1817 2294 2709 Size: 149 507 207 948 6 477 415 Fragments arranged by size: 948 507 477 415 207 149 6 BtsI GCAGTG_nn' Cuts at: 0 2355 2367 2709 Size: 2355 12 342 Fragments arranged by size: 2355 342 12 EaeI y'GGCC_r Cuts at: 0 517 1487 1814 2520 2709 Size: 517 970 327 706 189 Fragments arranged by size: 970 706 517 327 189 EarI CTCTTCn'nnn_ Cuts at: 0 389 989 1055 1162 2488 2709 Size: 389 600 66 107 1326 221 Fragments arranged by size: 1326 600 389 221 107 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 55 157 2709 Size: 55 102 2552 Fragments arranged by size: 2552 102 55 EcoICRI GAG'CTC Cuts at: 0 804 1566 2709 Size: 804 762 1143 Fragments arranged by size: 1143 804 762 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 301 505 946 1360 1384 1645 1737 Size: 116 185 204 441 414 24 261 92 Cuts at: 1737 1846 1892 1981 2137 2170 2512 2709 Size: 109 46 89 156 33 342 197 Fragments arranged by size: 441 414 342 261 204 197 185 156 116 109 92 89 46 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 194 856 1428 2709 Size: 194 662 572 1281 Fragments arranged by size: 1281 662 572 194 EcoO109I rG'GnC_Cy Cuts at: 0 523 870 1458 1924 1997 2015 2082 2121 Size: 523 347 588 466 73 18 67 39 Cuts at: 2121 2316 2709 Size: 195 393 Fragments arranged by size: 588 523 466 393 347 195 73 67 39 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 303 2709 Size: 303 2406 HaeII r_GCGC'y Cuts at: 0 377 606 1126 1879 2709 Size: 377 229 520 753 830 Fragments arranged by size: 830 753 520 377 229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1017 1049 1791 1823 2709 Size: 1017 32 742 32 886 Fragments arranged by size: 1017 886 742 32 32 HincII GTy'rAC Cuts at: 0 2187 2709 Size: 2187 522 HindIII A'AGCT_T Cuts at: 0 231 2709 Size: 231 2478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 222 938 1022 1130 1325 2709 Size: 222 716 84 108 195 1384 Fragments arranged by size: 1384 716 222 195 108 84 MscI TGG'CCA Cuts at: 0 1489 2709 Size: 1489 1220 MslI CAynn'nnrTG Cuts at: 0 1682 1816 2709 Size: 1682 134 893 Fragments arranged by size: 1682 893 134 MspA1I CmG'CkG Cuts at: 0 13 165 975 1290 1329 1338 1362 1371 Size: 13 152 810 315 39 9 24 9 Cuts at: 1371 1452 1623 1635 1698 1993 2115 2409 2510 Size: 81 171 12 63 295 122 294 101 Cuts at: 2510 2709 Size: 199 Fragments arranged by size: 810 315 295 294 199 171 152 122 101 81 63 39 24 13 12 9 9 NaeI GCC'GGC Cuts at: 0 136 2709 Size: 136 2573 NcoI C'CATG_G Cuts at: 0 863 1811 2294 2709 Size: 863 948 483 415 Fragments arranged by size: 948 863 483 415 NgoMIV G'CCGG_C Cuts at: 0 134 2709 Size: 134 2575 NheI G'CTAG_C Cuts at: 0 1497 2709 Size: 1497 1212 NsiI A_TGCA'T Cuts at: 0 567 2709 Size: 567 2142 PasI CC'CwG_GG Cuts at: 0 73 2709 Size: 73 2636 PflMI CCAn_nnn'nTGG Cuts at: 0 483 1623 1959 2148 2709 Size: 483 1140 336 189 561 Fragments arranged by size: 1140 561 483 336 189 PfoI T'CCnGG_A Cuts at: 0 2064 2709 Size: 2064 645 PmlI CAC'GTG Cuts at: 0 340 2709 Size: 340 2369 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2539 2571 2709 Size: 2539 32 138 Fragments arranged by size: 2539 138 32 PpuMI rG'GwC_Cy Cuts at: 0 1458 2316 2709 Size: 1458 858 393 Fragments arranged by size: 1458 858 393 PspOMI G'GGCC_C Cuts at: 0 1085 1923 2709 Size: 1085 838 786 Fragments arranged by size: 1085 838 786 PstI C_TGCA'G Cuts at: 0 623 905 992 1130 1289 1457 1607 1661 Size: 623 282 87 138 159 168 150 54 Cuts at: 1661 1673 2709 Size: 12 1036 Fragments arranged by size: 1036 623 282 168 159 150 138 87 54 12 PvuII CAG'CTG Cuts at: 0 13 165 1290 1338 1362 1452 1623 1698 Size: 13 152 1125 48 24 90 171 75 Cuts at: 1698 1993 2115 2409 2709 Size: 295 122 294 300 Fragments arranged by size: 1125 300 295 294 171 152 122 90 75 48 24 13 SacI G_AGCT'C Cuts at: 0 806 1568 2709 Size: 806 762 1141 Fragments arranged by size: 1141 806 762 SalI G'TCGA_C Cuts at: 0 2185 2709 Size: 2185 524 SanDI GG'GwC_CC Cuts at: 0 2316 2709 Size: 2316 393 SapI GCTCTTCn'nnn_ Cuts at: 0 989 1162 2709 Size: 989 173 1547 Fragments arranged by size: 1547 989 173 SbfI CC_TGCA'GG Cuts at: 0 992 2709 Size: 992 1717 SfcI C'TryA_G Cuts at: 0 619 640 901 988 1126 1285 1453 1603 Size: 619 21 261 87 138 159 168 150 Cuts at: 1603 1657 1669 2618 2709 Size: 54 12 949 91 Fragments arranged by size: 949 619 261 168 159 150 138 91 87 54 21 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 868 2709 Size: 868 1841 SmaI CCC'GGG Cuts at: 0 1579 1761 2709 Size: 1579 182 948 Fragments arranged by size: 1579 948 182 SmlI C'TyrA_G Cuts at: 0 1105 1730 2125 2243 2709 Size: 1105 625 395 118 466 Fragments arranged by size: 1105 625 466 395 118 StyI C'CwwG_G Cuts at: 0 863 1811 2294 2401 2709 Size: 863 948 483 107 308 Fragments arranged by size: 948 863 483 308 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2554 2709 Size: 2554 155 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2252 2284 2643 2675 2709 Size: 2252 32 359 32 34 Fragments arranged by size: 2252 359 34 32 32 XmaI C'CCGG_G Cuts at: 0 1577 1759 2709 Size: 1577 182 950 Fragments arranged by size: 1577 950 182 XmnI GAAnn'nnTTC Cuts at: 0 302 2709 Size: 302 2407 Enzymes that do cut: AarI AccI AcuI AflII AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI SapI SbfI SfcI SfiI SmaI SmlI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BclI BmgBI BsaBI BsaHI BsaWI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI NarI NdeI NotI NruI NspI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI ZraI