Restriction map for pF1KB8491
 (Linear) (Six-base) MAPSORT of: KIBB8491.seq  Check: 6937  from: 1  to: 2709



KIBB8491.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1536    2709 
   Size:       1536    1173
 
AccI GT'mk_AC

Cuts at:      0    2186    2709 
   Size:       2186     523
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    1846    2512    2709 
   Size:        116    1730     666     197

  Fragments arranged by size: 

                1730     666     197     116
 
AflII C'TTAA_G

Cuts at:      0    1105    2709 
   Size:       1105    1604
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     908    2709 
   Size:        908    1801
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2539    2571    2709 
   Size:       2539      32     138

  Fragments arranged by size: 

                2539     138      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     555     618    1125    1461    1485    1623    1959    2148 
   Size:        555      63     507     336      24     138     336     189

Cuts at:   2148    2709 
   Size:        561

  Fragments arranged by size: 

                 561     555     507     336     336     189     138      63
                  24
 
ApaI G_GGCC'C

Cuts at:      0    1089    1927    2709 
   Size:       1089     838     782

  Fragments arranged by size: 

                1089     838     782
 
ApoI r'AATT_y

Cuts at:      0    2130    2709 
   Size:       2130     579
 
AvaI C'yCGr_G

Cuts at:      0      34     155     626    1010    1577    1759    2447    2673 
   Size:         34     121     471     384     567     182     688     226

Cuts at:   2673    2709 
   Size:         36

  Fragments arranged by size: 

                 688     567     471     384     226     182     121      36
                  34
 
BamHI G'GATC_C

Cuts at:      0     357    1331    2709 
   Size:        357     974    1378

  Fragments arranged by size: 

                1378     974     357
 
BanI G'GyrC_C

Cuts at:      0    2663    2709 
   Size:       2663      46
 
BanII G_rGCy'C

Cuts at:      0      80     162     806    1089    1568    1927    1967    2463 
   Size:         80      82     644     283     479     359      40     496

Cuts at:   2463    2709 
   Size:        246

  Fragments arranged by size: 

                 644     496     479     359     283     246      82      80
                  40
 
BbvCI CC'TCA_GC

Cuts at:      0     130    1553    2471    2709 
   Size:        130    1423     918     238

  Fragments arranged by size: 

                1423     918     238     130
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1251    2709 
   Size:       1251    1458
 
BfrBI ATG'CAT

Cuts at:      0     565    2709 
   Size:        565    2144
 
BglI GCCn_nnn'nGGC

Cuts at:      0     868    2709 
   Size:        868    1841
 
BglII A'GATC_T

Cuts at:      0    1905    2709 
   Size:       1905     804
 
BlpI GC'TnA_GC

Cuts at:      0     471     752    1702    2111    2709 
   Size:        471     281     950     409     598

  Fragments arranged by size: 

                 950     598     471     409     281
 
Bme1580I G_kGCm'C

Cuts at:      0    1089    1927    2162    2666    2709 
   Size:       1089     838     235     504      43

  Fragments arranged by size: 

                1089     838     504     235      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      17    1689    2709 
   Size:         17    1672    1020

  Fragments arranged by size: 

                1672    1020      17
 
BmtI G_CTAG'C

Cuts at:      0    1501    2709 
   Size:       1501    1208
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1160    2346    2607    2709 
   Size:       1160    1186     261     102

  Fragments arranged by size: 

                1186    1160     261     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     505     946    1360    1384    1645    1737    1892 
   Size:        301     204     441     414      24     261      92     155

Cuts at:   1892    1981    2137    2170    2709 
   Size:         89     156      33     539

  Fragments arranged by size: 

                 539     441     414     301     261     204     156     155
                  92      89      33      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     130    1397    1553    2289    2321    2471    2709 
   Size:        130    1267     156     736      32     150     238

  Fragments arranged by size: 

                1267     736     238     156     150     130      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1715    2110    2228    2709 
   Size:       1715     395     118     481

  Fragments arranged by size: 

                1715     481     395     118
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1192    2709 
   Size:       1192    1517
 
BsaAI yAC'GTr

Cuts at:      0     340    2254    2709 
   Size:        340    1914     455

  Fragments arranged by size: 

                1914     455     340
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     478     508    1791    1821    2539    2569    2709 
   Size:        478      30    1283      30     718      30     140

  Fragments arranged by size: 

                1283     718     478     140      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     502     943    1147    1642    1727    1798    1803    1890 
   Size:        502     441     204     495      85      71       5      87

Cuts at:   1890    2322    2700    2709 
   Size:        432     378       9

  Fragments arranged by size: 

                 502     495     441     432     378     204      87      85
                  71       9       5
 
BseYI C'CCAG_C

Cuts at:      0     107     161    1015    1088    1202    1993    2356    2453 
   Size:        107      54     854      73     114     791     363      97

Cuts at:   2453    2709 
   Size:        256

  Fragments arranged by size: 

                 854     791     363     256     114     107      97      73
                  54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    1327    1378    1991    2709 
   Size:       1243      84      51     613     718

  Fragments arranged by size: 

                1243     718     613      84      51
 
BsiEI CG_ry'CG

Cuts at:      0     675    2709 
   Size:        675    2034
 
BsiHKAI G_wGCw'C

Cuts at:      0     806    1100    1568    2709 
   Size:        806     294     468    1141

  Fragments arranged by size: 

                1141     806     468     294
 
BsmI GAATG_Cn'

Cuts at:      0    1944    2709 
   Size:       1944     765
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     381    2709 
   Size:        381    2328
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     162     806    1089    1100    1568    1927    1967 
   Size:         80      82     644     283      11     468     359      40

Cuts at:   1967    2162    2463    2666    2709 
   Size:        195     301     203      43

  Fragments arranged by size: 

                 644     468     359     301     283     203     195      82
                  80      43      40      11
 
BspHI T'CATG_A

Cuts at:      0     192    2709 
   Size:        192    2517
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1536    2709 
   Size:       1536    1173
 
BsrBI CCG'CTC

Cuts at:      0     756     999    1383    2709 
   Size:        756     243     384    1326

  Fragments arranged by size: 

                1326     756     384     243
 
BsrFI r'CCGG_y

Cuts at:      0     134     740    2709 
   Size:        134     606    1969

  Fragments arranged by size: 

                1969     606     134
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1665    2100    2145    2709 
   Size:       1665     435      45     564

  Fragments arranged by size: 

                1665     564     435      45
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1818    2709 
   Size:       1818     891
 
BstYI r'GATC_y

Cuts at:      0      45     357    1331    1905    2061    2475    2570    2709 
   Size:         45     312     974     574     156     414      95     139

  Fragments arranged by size: 

                 974     574     414     312     156     139      95      45
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1429    1766    2054    2709 
   Size:       1429     337     288     655

  Fragments arranged by size: 

                1429     655     337     288
 
BtgI C'CryG_G

Cuts at:      0     149     656     863    1811    1817    2294    2709 
   Size:        149     507     207     948       6     477     415

  Fragments arranged by size: 

                 948     507     477     415     207     149       6
 
BtsI GCAGTG_nn'

Cuts at:      0    2355    2367    2709 
   Size:       2355      12     342

  Fragments arranged by size: 

                2355     342      12
 
EaeI y'GGCC_r

Cuts at:      0     517    1487    1814    2520    2709 
   Size:        517     970     327     706     189

  Fragments arranged by size: 

                 970     706     517     327     189
 
EarI CTCTTCn'nnn_

Cuts at:      0     389     989    1055    1162    2488    2709 
   Size:        389     600      66     107    1326     221

  Fragments arranged by size: 

                1326     600     389     221     107      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55     157    2709 
   Size:         55     102    2552

  Fragments arranged by size: 

                2552     102      55
 
EcoICRI GAG'CTC

Cuts at:      0     804    1566    2709 
   Size:        804     762    1143

  Fragments arranged by size: 

                1143     804     762
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     301     505     946    1360    1384    1645    1737 
   Size:        116     185     204     441     414      24     261      92

Cuts at:   1737    1846    1892    1981    2137    2170    2512    2709 
   Size:        109      46      89     156      33     342     197

  Fragments arranged by size: 

                 441     414     342     261     204     197     185     156
                 116     109      92      89      46      33      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     194     856    1428    2709 
   Size:        194     662     572    1281

  Fragments arranged by size: 

                1281     662     572     194
 
EcoO109I rG'GnC_Cy

Cuts at:      0     523     870    1458    1924    1997    2015    2082    2121 
   Size:        523     347     588     466      73      18      67      39

Cuts at:   2121    2316    2709 
   Size:        195     393

  Fragments arranged by size: 

                 588     523     466     393     347     195      73      67
                  39      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     303    2709 
   Size:        303    2406
 
HaeII r_GCGC'y

Cuts at:      0     377     606    1126    1879    2709 
   Size:        377     229     520     753     830

  Fragments arranged by size: 

                 830     753     520     377     229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1017    1049    1791    1823    2709 
   Size:       1017      32     742      32     886

  Fragments arranged by size: 

                1017     886     742      32      32
 
HincII GTy'rAC

Cuts at:      0    2187    2709 
   Size:       2187     522
 
HindIII A'AGCT_T

Cuts at:      0     231    2709 
   Size:        231    2478
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     938    1022    1130    1325    2709 
   Size:        222     716      84     108     195    1384

  Fragments arranged by size: 

                1384     716     222     195     108      84
 
MscI TGG'CCA

Cuts at:      0    1489    2709 
   Size:       1489    1220
 
MslI CAynn'nnrTG

Cuts at:      0    1682    1816    2709 
   Size:       1682     134     893

  Fragments arranged by size: 

                1682     893     134
 
MspA1I CmG'CkG

Cuts at:      0      13     165     975    1290    1329    1338    1362    1371 
   Size:         13     152     810     315      39       9      24       9

Cuts at:   1371    1452    1623    1635    1698    1993    2115    2409    2510 
   Size:         81     171      12      63     295     122     294     101

Cuts at:   2510    2709 
   Size:        199

  Fragments arranged by size: 

                 810     315     295     294     199     171     152     122
                 101      81      63      39      24      13      12       9
                   9
 
NaeI GCC'GGC

Cuts at:      0     136    2709 
   Size:        136    2573
 
NcoI C'CATG_G

Cuts at:      0     863    1811    2294    2709 
   Size:        863     948     483     415

  Fragments arranged by size: 

                 948     863     483     415
 
NgoMIV G'CCGG_C

Cuts at:      0     134    2709 
   Size:        134    2575
 
NheI G'CTAG_C

Cuts at:      0    1497    2709 
   Size:       1497    1212
 
NsiI A_TGCA'T

Cuts at:      0     567    2709 
   Size:        567    2142
 
PasI CC'CwG_GG

Cuts at:      0      73    2709 
   Size:         73    2636
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     483    1623    1959    2148    2709 
   Size:        483    1140     336     189     561

  Fragments arranged by size: 

                1140     561     483     336     189
 
PfoI T'CCnGG_A

Cuts at:      0    2064    2709 
   Size:       2064     645
 
PmlI CAC'GTG

Cuts at:      0     340    2709 
   Size:        340    2369
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2539    2571    2709 
   Size:       2539      32     138

  Fragments arranged by size: 

                2539     138      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1458    2316    2709 
   Size:       1458     858     393

  Fragments arranged by size: 

                1458     858     393
 
PspOMI G'GGCC_C

Cuts at:      0    1085    1923    2709 
   Size:       1085     838     786

  Fragments arranged by size: 

                1085     838     786
 
PstI C_TGCA'G

Cuts at:      0     623     905     992    1130    1289    1457    1607    1661 
   Size:        623     282      87     138     159     168     150      54

Cuts at:   1661    1673    2709 
   Size:         12    1036

  Fragments arranged by size: 

                1036     623     282     168     159     150     138      87
                  54      12
 
PvuII CAG'CTG

Cuts at:      0      13     165    1290    1338    1362    1452    1623    1698 
   Size:         13     152    1125      48      24      90     171      75

Cuts at:   1698    1993    2115    2409    2709 
   Size:        295     122     294     300

  Fragments arranged by size: 

                1125     300     295     294     171     152     122      90
                  75      48      24      13
 
SacI G_AGCT'C

Cuts at:      0     806    1568    2709 
   Size:        806     762    1141

  Fragments arranged by size: 

                1141     806     762
 
SalI G'TCGA_C

Cuts at:      0    2185    2709 
   Size:       2185     524
 
SanDI GG'GwC_CC

Cuts at:      0    2316    2709 
   Size:       2316     393
 
SapI GCTCTTCn'nnn_

Cuts at:      0     989    1162    2709 
   Size:        989     173    1547

  Fragments arranged by size: 

                1547     989     173
 
SbfI CC_TGCA'GG

Cuts at:      0     992    2709 
   Size:        992    1717
 
SfcI C'TryA_G

Cuts at:      0     619     640     901     988    1126    1285    1453    1603 
   Size:        619      21     261      87     138     159     168     150

Cuts at:   1603    1657    1669    2618    2709 
   Size:         54      12     949      91

  Fragments arranged by size: 

                 949     619     261     168     159     150     138      91
                  87      54      21      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     868    2709 
   Size:        868    1841
 
SmaI CCC'GGG

Cuts at:      0    1579    1761    2709 
   Size:       1579     182     948

  Fragments arranged by size: 

                1579     948     182
 
SmlI C'TyrA_G

Cuts at:      0    1105    1730    2125    2243    2709 
   Size:       1105     625     395     118     466

  Fragments arranged by size: 

                1105     625     466     395     118
 
StyI C'CwwG_G

Cuts at:      0     863    1811    2294    2401    2709 
   Size:        863     948     483     107     308

  Fragments arranged by size: 

                 948     863     483     308     107
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2554    2709 
   Size:       2554     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2252    2284    2643    2675    2709 
   Size:       2252      32     359      32      34

  Fragments arranged by size: 

                2252     359      34      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1577    1759    2709 
   Size:       1577     182     950

  Fragments arranged by size: 

                1577     950     182
 
XmnI GAAnn'nnTTC

Cuts at:      0     302    2709 
   Size:        302    2407
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII    BbvCI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     NsiI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     SalI    SanDI
     SapI     SbfI     SfcI     SfiI     SmaI     SmlI     StyI    TaqII
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
     BcgI     BclI    BmgBI    BsaBI    BsaHI    BsaWI    BsiWI    BspEI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NotI     NruI     NspI     PacI     PciI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     ScaI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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