Restriction map for
pF1KB8492
(Linear) (Six-base) MAPSORT of: KIBB8492.seq Check: 2755 from: 1 to: 2574
KIBB8492.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1079 2250 2574
Size: 1079 1171 324
Fragments arranged by size:
1171 1079 324
AatII G_ACGT'C
Cuts at: 0 2111 2120 2574
Size: 2111 9 454
Fragments arranged by size:
2111 454 9
AccI GT'mk_AC
Cuts at: 0 17 326 2574
Size: 17 309 2248
Fragments arranged by size:
2248 309 17
Acc65I G'GTAC_C
Cuts at: 0 628 2574
Size: 628 1946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1027 1294 1351 1591 1693 2574
Size: 694 333 267 57 240 102 881
Fragments arranged by size:
881 694 333 267 240 102 57
AflIII A'CryG_T
Cuts at: 0 62 2306 2574
Size: 62 2244 268
Fragments arranged by size:
2244 268 62
AhdI GACnn_n'nnGTC
Cuts at: 0 393 2400 2574
Size: 393 2007 174
Fragments arranged by size:
2007 393 174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 455 1018 2196 2574
Size: 455 563 1178 378
Fragments arranged by size:
1178 563 455 378
AlwNI CAG_nnn'CTG
Cuts at: 0 1434 2457 2574
Size: 1434 1023 117
Fragments arranged by size:
1434 1023 117
ApaI G_GGCC'C
Cuts at: 0 1127 1295 1961 2574
Size: 1127 168 666 613
Fragments arranged by size:
1127 666 613 168
ApoI r'AATT_y
Cuts at: 0 915 2574
Size: 915 1659
AvaI C'yCGr_G
Cuts at: 0 146 316 511 1251 2574
Size: 146 170 195 740 1323
Fragments arranged by size:
1323 740 195 170 146
BanI G'GyrC_C
Cuts at: 0 283 628 1203 1447 1642 1966 2332 2574
Size: 283 345 575 244 195 324 366 242
Fragments arranged by size:
575 366 345 324 283 244 242 195
BanII G_rGCy'C
Cuts at: 0 242 512 521 1112 1127 1295 1379 1883
Size: 242 270 9 591 15 168 84 504
Cuts at: 1883 1961 2228 2574
Size: 78 267 346
Fragments arranged by size:
591 504 346 270 267 242 168 84
78 15 9
BbeI G_GCGC'C
Cuts at: 0 1646 2574
Size: 1646 928
BbsI GAAGACnn'nnnn_
Cuts at: 0 1239 1447 2574
Size: 1239 208 1127
Fragments arranged by size:
1239 1127 208
BbvCI CC'TCA_GC
Cuts at: 0 1931 2574
Size: 1931 643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1123 1157 2574
Size: 1123 34 1417
Fragments arranged by size:
1417 1123 34
BclI T'GATC_A
Cuts at: 0 1058 2574
Size: 1058 1516
BglI GCCn_nnn'nGGC
Cuts at: 0 599 745 1418 1528 2574
Size: 599 146 673 110 1046
Fragments arranged by size:
1046 673 599 146 110
BglII A'GATC_T
Cuts at: 0 1664 1943 2574
Size: 1664 279 631
Fragments arranged by size:
1664 631 279
Bme1580I G_kGCm'C
Cuts at: 0 1127 1295 1450 1961 2574
Size: 1127 168 155 511 613
Fragments arranged by size:
1127 613 511 168 155
BmgBI CAC'GTC
Cuts at: 0 1926 2574
Size: 1926 648
BmrI ACTGGGnnnn_n'
Cuts at: 0 2424 2574
Size: 2424 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 526 1681 1702 2574
Size: 514 12 1155 21 872
Fragments arranged by size:
1155 872 514 21 12
Bpu10I CC'TnA_GC
Cuts at: 0 1931 2574
Size: 1931 643
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1867 2574
Size: 1867 707
BsaI GGTCTCn'nnnn_
Cuts at: 0 1266 2459 2503 2574
Size: 1266 1193 44 71
Fragments arranged by size:
1266 1193 71 44
BsaAI yAC'GTr
Cuts at: 0 81 1371 2307 2574
Size: 81 1290 936 267
Fragments arranged by size:
1290 936 267 81
BsaBI GATnn'nnATC
Cuts at: 0 1063 2574
Size: 1063 1511
BsaHI Gr'CG_yC
Cuts at: 0 1043 1643 2108 2117 2135 2574
Size: 1043 600 465 9 18 439
Fragments arranged by size:
1043 600 465 439 18 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 712 742 1060 1090 2233 2263 2574
Size: 712 30 318 30 1143 30 311
Fragments arranged by size:
1143 712 318 311 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 324 529 943 1630 1699 2095 2329 2574
Size: 324 205 414 687 69 396 234 245
Fragments arranged by size:
687 414 396 324 245 234 205 69
BseYI C'CCAG_C
Cuts at: 0 801 2207 2491 2574
Size: 801 1406 284 83
Fragments arranged by size:
1406 801 284 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1621 1639 2026 2110 2574
Size: 430 1191 18 387 84 464
Fragments arranged by size:
1191 464 430 387 84 18
BsiEI CG_ry'CG
Cuts at: 0 1727 2574
Size: 1727 847
BsiHKAI G_wGCw'C
Cuts at: 0 242 521 1112 1469 1769 1883 2574
Size: 242 279 591 357 300 114 691
Fragments arranged by size:
691 591 357 300 279 242 114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1034 1734 1735 2574
Size: 1034 700 1 839
Fragments arranged by size:
1034 839 700 1
Bsp1286I G_dGCh'C
Cuts at: 0 242 512 521 1112 1127 1295 1379 1450
Size: 242 270 9 591 15 168 84 71
Cuts at: 1450 1469 1769 1883 1961 2228 2574
Size: 19 300 114 78 267 346
Fragments arranged by size:
591 346 300 270 267 242 168 114
84 78 71 19 15 9
BspHI T'CATG_A
Cuts at: 0 608 911 2574
Size: 608 303 1663
Fragments arranged by size:
1663 608 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1079 1556 2250 2574
Size: 1079 477 694 324
Fragments arranged by size:
1079 694 477 324
BsrBI CCG'CTC
Cuts at: 0 590 744 2574
Size: 590 154 1830
Fragments arranged by size:
1830 590 154
BsrDI GCAATG_nn'
Cuts at: 0 437 2574
Size: 437 2137
BsrFI r'CCGG_y
Cuts at: 0 286 1645 1799 1969 2041 2329 2574
Size: 286 1359 154 170 72 288 245
Fragments arranged by size:
1359 288 286 245 170 154 72
BsrGI T'GTAC_A
Cuts at: 0 1805 2574
Size: 1805 769
BssHII G'CGCG_C
Cuts at: 0 1894 2574
Size: 1894 680
BssSI C'ACGA_G
Cuts at: 0 1414 2574
Size: 1414 1160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 866 1479 2457 2574
Size: 866 613 978 117
Fragments arranged by size:
978 866 613 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1560 2346 2499 2574
Size: 1560 786 153 75
Fragments arranged by size:
1560 786 153 75
BstYI r'GATC_y
Cuts at: 0 23 1664 1943 2243 2453 2574
Size: 23 1641 279 300 210 121
Fragments arranged by size:
1641 300 279 210 121 23
Bsu36I CC'TnA_GG
Cuts at: 0 2398 2574
Size: 2398 176
BtgI C'CryG_G
Cuts at: 0 87 596 632 654 1145 1523 1595 2145
Size: 87 509 36 22 491 378 72 550
Cuts at: 2145 2574
Size: 429
Fragments arranged by size:
550 509 491 429 378 87 72 36
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 57 120 1620 2574
Size: 57 63 1500 954
Fragments arranged by size:
1500 954 63 57
BtsI GCAGTG_nn'
Cuts at: 0 1609 2574
Size: 1609 965
ClaI AT'CG_AT
Cuts at: 0 614 1850 2574
Size: 614 1236 724
Fragments arranged by size:
1236 724 614
DraIII CAC_nnn'GTG
Cuts at: 0 2077 2574
Size: 2077 497
DrdI GACnn_nn'nnGTC
Cuts at: 0 1726 2574
Size: 1726 848
EaeI y'GGCC_r
Cuts at: 0 698 1520 1580 1838 1904 2039 2327 2415
Size: 698 822 60 258 66 135 288 88
Cuts at: 2415 2574
Size: 159
Fragments arranged by size:
822 698 288 258 159 135 88 66
60
EarI CTCTTCn'nnn_
Cuts at: 0 235 1748 2574
Size: 235 1513 826
Fragments arranged by size:
1513 826 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1857 2521 2574
Size: 1857 664 53
Fragments arranged by size:
1857 664 53
EcoICRI GAG'CTC
Cuts at: 0 240 519 1110 1881 2574
Size: 240 279 591 771 693
Fragments arranged by size:
771 693 591 279 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 526 694 1027 1294 1351 1591 1681
Size: 514 12 168 333 267 57 240 90
Cuts at: 1681 1693 1702 2574
Size: 12 9 872
Fragments arranged by size:
872 514 333 267 240 168 90 57
12 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1006 1117 1684 1840 2574
Size: 1006 111 567 156 734
Fragments arranged by size:
1006 734 567 156 111
EcoO109I rG'GnC_Cy
Cuts at: 0 733 1123 1124 1174 1291 1320 1677 2526
Size: 733 390 1 50 117 29 357 849
Cuts at: 2526 2574
Size: 48
Fragments arranged by size:
849 733 390 357 117 50 48 29
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1195 2574
Size: 1195 1379
HaeII r_GCGC'y
Cuts at: 0 1646 2181 2574
Size: 1646 535 393
Fragments arranged by size:
1646 535 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 379 411 1616 1648 2235 2267
Size: 319 32 28 32 1205 32 587 32
Cuts at: 2267 2386 2418 2574
Size: 119 32 156
Fragments arranged by size:
1205 587 319 156 119 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 215 327 2370 2574
Size: 215 112 2043 204
Fragments arranged by size:
2043 215 204 112
KasI G'GCGC_C
Cuts at: 0 1642 2574
Size: 1642 932
KpnI G_GTAC'C
Cuts at: 0 632 2574
Size: 632 1942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1238 1613 2429 2574
Size: 1238 375 816 145
Fragments arranged by size:
1238 816 375 145
MscI TGG'CCA
Cuts at: 0 700 1582 2574
Size: 700 882 992
Fragments arranged by size:
992 882 700
MslI CAynn'nnrTG
Cuts at: 0 1483 2338 2344 2574
Size: 1483 855 6 230
Fragments arranged by size:
1483 855 230 6
MspA1I CmG'CkG
Cuts at: 0 504 873 1025 1103 1890 2147 2392 2574
Size: 504 369 152 78 787 257 245 182
Fragments arranged by size:
787 504 369 257 245 182 152 78
NaeI GCC'GGC
Cuts at: 0 288 1647 2043 2331 2574
Size: 288 1359 396 288 243
Fragments arranged by size:
1359 396 288 288 243
NarI GG'CG_CC
Cuts at: 0 1643 2574
Size: 1643 931
NcoI C'CATG_G
Cuts at: 0 87 596 1145 1595 2574
Size: 87 509 549 450 979
Fragments arranged by size:
979 549 509 450 87
NgoMIV G'CCGG_C
Cuts at: 0 286 1645 2041 2329 2574
Size: 286 1359 396 288 245
Fragments arranged by size:
1359 396 288 286 245
NspI r_CATG'y
Cuts at: 0 66 325 1482 2091 2574
Size: 66 259 1157 609 483
Fragments arranged by size:
1157 609 483 259 66
PasI CC'CwG_GG
Cuts at: 0 1306 2574
Size: 1306 1268
PciI A'CATG_T
Cuts at: 0 62 2574
Size: 62 2512
PflMI CCAn_nnn'nTGG
Cuts at: 0 1434 2502 2574
Size: 1434 1068 72
Fragments arranged by size:
1434 1068 72
PfoI T'CCnGG_A
Cuts at: 0 878 2561 2574
Size: 878 1683 13
Fragments arranged by size:
1683 878 13
PmlI CAC'GTG
Cuts at: 0 81 2307 2574
Size: 81 2226 267
Fragments arranged by size:
2226 267 81
PpuMI rG'GwC_Cy
Cuts at: 0 1320 1677 2574
Size: 1320 357 897
Fragments arranged by size:
1320 897 357
PshAI GACnn'nnGTC
Cuts at: 0 1220 2574
Size: 1220 1354
PspOMI G'GGCC_C
Cuts at: 0 1123 1291 1957 2574
Size: 1123 168 666 617
Fragments arranged by size:
1123 666 617 168
PstI C_TGCA'G
Cuts at: 0 503 2574
Size: 503 2071
PvuII CAG'CTG
Cuts at: 0 504 873 2574
Size: 504 369 1701
Fragments arranged by size:
1701 504 369
SacI G_AGCT'C
Cuts at: 0 242 521 1112 1883 2574
Size: 242 279 591 771 691
Fragments arranged by size:
771 691 591 279 242
SacII CC_GC'GG
Cuts at: 0 2148 2574
Size: 2148 426
SalI G'TCGA_C
Cuts at: 0 325 2574
Size: 325 2249
SfcI C'TryA_G
Cuts at: 0 499 2574
Size: 499 2075
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1528 2574
Size: 1528 1046
SfoI GGC'GCC
Cuts at: 0 1644 2574
Size: 1644 930
SgrAI Cr'CCGG_yG
Cuts at: 0 1645 2574
Size: 1645 929
SmaI CCC'GGG
Cuts at: 0 148 318 2574
Size: 148 170 2256
Fragments arranged by size:
2256 170 148
SmlI C'TyrA_G
Cuts at: 0 1882 2574
Size: 1882 692
SrfI GCCC'GGGC
Cuts at: 0 148 2574
Size: 148 2426
StuI AGG'CCT
Cuts at: 0 2356 2574
Size: 2356 218
StyI C'CwwG_G
Cuts at: 0 87 596 713 1145 1595 1997 2060 2574
Size: 87 509 117 432 450 402 63 514
Fragments arranged by size:
514 509 450 432 402 117 87 63
TatI w'GTAC_w
Cuts at: 0 1805 2574
Size: 1805 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 2233 2265 2574
Size: 1060 32 1141 32 309
Fragments arranged by size:
1141 1060 309 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1723 2574
Size: 1723 851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 85 2235 2499 2574
Size: 85 2150 264 75
Fragments arranged by size:
2150 264 85 75
XmaI C'CCGG_G
Cuts at: 0 146 316 2574
Size: 146 170 2258
Fragments arranged by size:
2258 170 146
ZraI GAC'GTC
Cuts at: 0 2109 2118 2574
Size: 2109 9 456
Fragments arranged by size:
2109 456 9
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AlfI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI SfcI SfiI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TatI TstI Tth111I
XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AleI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BlpI
BmtI BplI BsaWI BsiWI BsmI BspEI BstBI BstEII
BstZ17I CspCI DraI EagI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SanDI SapI SbfI ScaI SexAI SgfI
SnaBI SpeI SphI SspI SwaI TaqII XbaI XhoI
XmnI