Restriction map for
pF1KB8500
(Linear) (Six-base) MAPSORT of: KIBB8500.seq Check: 605 from: 1 to: 2586
KIBB8500.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 2586
Size: 78 2508
AatII G_ACGT'C
Cuts at: 0 2348 2586
Size: 2348 238
AccI GT'mk_AC
Cuts at: 0 926 2586
Size: 926 1660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 2586
Size: 1358 1228
AflII C'TTAA_G
Cuts at: 0 172 2586
Size: 172 2414
AflIII A'CryG_T
Cuts at: 0 932 1241 1593 1796 2586
Size: 932 309 352 203 790
Fragments arranged by size:
932 790 352 309 203
AleI CACnn'nnGTG
Cuts at: 0 1498 2586
Size: 1498 1088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 921 2435 2586
Size: 921 1514 151
Fragments arranged by size:
1514 921 151
AlwNI CAG_nnn'CTG
Cuts at: 0 31 1128 1541 2284 2432 2586
Size: 31 1097 413 743 148 154
Fragments arranged by size:
1097 743 413 154 148 31
ApaLI G'TGCA_C
Cuts at: 0 344 2586
Size: 344 2242
ApoI r'AATT_y
Cuts at: 0 514 1025 1681 1748 2586
Size: 514 511 656 67 838
Fragments arranged by size:
838 656 514 511 67
AseI AT'TA_AT
Cuts at: 0 1832 2586
Size: 1832 754
AvaI C'yCGr_G
Cuts at: 0 18 1479 2402 2586
Size: 18 1461 923 184
Fragments arranged by size:
1461 923 184 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 934 967 2586
Size: 934 33 1619
Fragments arranged by size:
1619 934 33
BanI G'GyrC_C
Cuts at: 0 148 1378 2586
Size: 148 1230 1208
Fragments arranged by size:
1230 1208 148
BanII G_rGCy'C
Cuts at: 0 19 337 1336 1585 2586
Size: 19 318 999 249 1001
Fragments arranged by size:
1001 999 318 249 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1355 2586
Size: 1355 1231
BbvCI CC'TCA_GC
Cuts at: 0 1697 2586
Size: 1697 889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2254 2288 2586
Size: 2254 34 298
Fragments arranged by size:
2254 298 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 962 2586
Size: 962 1624
BclI T'GATC_A
Cuts at: 0 411 2489 2586
Size: 411 2078 97
Fragments arranged by size:
2078 411 97
BfrBI ATG'CAT
Cuts at: 0 568 1161 2586
Size: 568 593 1425
Fragments arranged by size:
1425 593 568
BglII A'GATC_T
Cuts at: 0 1405 2586
Size: 1405 1181
BlpI GC'TnA_GC
Cuts at: 0 2368 2586
Size: 2368 218
Bme1580I G_kGCm'C
Cuts at: 0 348 1487 1850 2586
Size: 348 1139 363 736
Fragments arranged by size:
1139 736 363 348
BmgBI CAC'GTC
Cuts at: 0 1242 2586
Size: 1242 1344
BmrI ACTGGGnnnn_n'
Cuts at: 0 1436 2586
Size: 1436 1150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1494 2586
Size: 1494 1092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1244 1301 1982 2137 2261 2409 2586
Size: 995 249 57 681 155 124 148 177
Fragments arranged by size:
995 681 249 177 155 148 124 57
Bpu10I CC'TnA_GC
Cuts at: 0 35 1697 2586
Size: 35 1662 889
Fragments arranged by size:
1662 889 35
BsaHI Gr'CG_yC
Cuts at: 0 1397 2345 2586
Size: 1397 948 241
Fragments arranged by size:
1397 948 241
BsaWI w'CCGG_w
Cuts at: 0 1672 2586
Size: 1672 914
BseYI C'CCAG_C
Cuts at: 0 661 894 2586
Size: 661 233 1692
Fragments arranged by size:
1692 661 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 2586
Size: 496 2090
BsiHKAI G_wGCw'C
Cuts at: 0 19 337 348 2153 2586
Size: 19 318 11 1805 433
Fragments arranged by size:
1805 433 318 19 11
BsmI GAATG_Cn'
Cuts at: 0 994 2586
Size: 994 1592
Bsp1286I G_dGCh'C
Cuts at: 0 19 337 348 1336 1487 1585 1850 2153
Size: 19 318 11 988 151 98 265 303
Cuts at: 2153 2586
Size: 433
Fragments arranged by size:
988 433 318 303 265 151 98 19
11
BspHI T'CATG_A
Cuts at: 0 1899 2586
Size: 1899 687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 78 379 2586
Size: 39 39 301 2207
Fragments arranged by size:
2207 301 39 39
BsrBI CCG'CTC
Cuts at: 0 1462 2586
Size: 1462 1124
BsrFI r'CCGG_y
Cuts at: 0 878 2393 2586
Size: 878 1515 193
Fragments arranged by size:
1515 878 193
BssSI C'ACGA_G
Cuts at: 0 913 2586
Size: 913 1673
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1128 1541 2148 2586
Size: 1029 99 413 607 438
Fragments arranged by size:
1029 607 438 413 99
BstBI TT'CG_AA
Cuts at: 0 239 2586
Size: 239 2347
BstYI r'GATC_y
Cuts at: 0 189 1405 2586
Size: 189 1216 1181
Fragments arranged by size:
1216 1181 189
BtgI C'CryG_G
Cuts at: 0 1184 1499 2105 2532 2586
Size: 1184 315 606 427 54
Fragments arranged by size:
1184 606 427 315 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 368 2541 2586
Size: 368 2173 45
Fragments arranged by size:
2173 368 45
BtsI GCAGTG_nn'
Cuts at: 0 107 1126 1427 1539 2586
Size: 107 1019 301 112 1047
Fragments arranged by size:
1047 1019 301 112 107
ClaI AT'CG_AT
Cuts at: 0 2264 2586
Size: 2264 322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 141 176 198 233 2586
Size: 141 35 22 35 2353
Fragments arranged by size:
2353 141 35 35 22
DraI TTT'AAA
Cuts at: 0 1064 1737 2586
Size: 1064 673 849
Fragments arranged by size:
1064 849 673
DraIII CAC_nnn'GTG
Cuts at: 0 343 2148 2586
Size: 343 1805 438
Fragments arranged by size:
1805 438 343
DrdI GACnn_nn'nnGTC
Cuts at: 0 1396 2586
Size: 1396 1190
EaeI y'GGCC_r
Cuts at: 0 1559 2586
Size: 1559 1027
EarI CTCTTCn'nnn_
Cuts at: 0 752 1280 2586
Size: 752 528 1306
Fragments arranged by size:
1306 752 528
EcoICRI GAG'CTC
Cuts at: 0 17 335 2586
Size: 17 318 2251
Fragments arranged by size:
2251 318 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1244 1301 1358 1982 2137 2261 2409
Size: 995 249 57 57 624 155 124 148
Cuts at: 2409 2586
Size: 177
Fragments arranged by size:
995 624 249 177 155 148 124 57
57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 2586
Size: 295 2291
EcoRV GAT'ATC
Cuts at: 0 999 2082 2586
Size: 999 1083 504
Fragments arranged by size:
1083 999 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1768 2139 2265 2586
Size: 1768 371 126 321
Fragments arranged by size:
1768 371 321 126
HaeII r_GCGC'y
Cuts at: 0 1618 2586
Size: 1618 968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 396 428 1416 1448 1567 1599
Size: 46 32 318 32 988 32 119 32
Cuts at: 1599 2208 2240 2586
Size: 609 32 346
Fragments arranged by size:
988 609 346 318 119 46 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1101 2586
Size: 1101 1485
HindIII A'AGCT_T
Cuts at: 0 169 1045 2586
Size: 169 876 1541
Fragments arranged by size:
1541 876 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 819 2586
Size: 372 447 1767
Fragments arranged by size:
1767 447 372
MslI CAynn'nnrTG
Cuts at: 0 1498 2497 2586
Size: 1498 999 89
Fragments arranged by size:
1498 999 89
MspA1I CmG'CkG
Cuts at: 0 732 1168 2586
Size: 732 436 1418
Fragments arranged by size:
1418 732 436
NaeI GCC'GGC
Cuts at: 0 2395 2586
Size: 2395 191
NcoI C'CATG_G
Cuts at: 0 1184 2532 2586
Size: 1184 1348 54
Fragments arranged by size:
1348 1184 54
NgoMIV G'CCGG_C
Cuts at: 0 2393 2586
Size: 2393 193
NsiI A_TGCA'T
Cuts at: 0 570 1163 2586
Size: 570 593 1423
Fragments arranged by size:
1423 593 570
NspI r_CATG'y
Cuts at: 0 568 936 1165 1597 1800 2104 2586
Size: 568 368 229 432 203 304 482
Fragments arranged by size:
568 482 432 368 304 229 203
PasI CC'CwG_GG
Cuts at: 0 1570 2380 2586
Size: 1570 810 206
Fragments arranged by size:
1570 810 206
PciI A'CATG_T
Cuts at: 0 932 1593 1796 2586
Size: 932 661 203 790
Fragments arranged by size:
932 790 661 203
PfoI T'CCnGG_A
Cuts at: 0 1959 2586
Size: 1959 627
PshAI GACnn'nnGTC
Cuts at: 0 466 1769 2586
Size: 466 1303 817
Fragments arranged by size:
1303 817 466
PsiI TTA'TAA
Cuts at: 0 2059 2586
Size: 2059 527
PspXI vC'TCGA_Gb
Cuts at: 0 18 2586
Size: 18 2568
PstI C_TGCA'G
Cuts at: 0 910 1546 2138 2586
Size: 910 636 592 448
Fragments arranged by size:
910 636 592 448
PvuII CAG'CTG
Cuts at: 0 732 2586
Size: 732 1854
SacI G_AGCT'C
Cuts at: 0 19 337 2586
Size: 19 318 2249
Fragments arranged by size:
2249 318 19
SfcI C'TryA_G
Cuts at: 0 906 1542 2134 2586
Size: 906 636 592 452
Fragments arranged by size:
906 636 592 452
SmlI C'TyrA_G
Cuts at: 0 18 172 2586
Size: 18 154 2414
Fragments arranged by size:
2414 154 18
SpeI A'CTAG_T
Cuts at: 0 2314 2586
Size: 2314 272
SphI G_CATG'C
Cuts at: 0 1165 2104 2586
Size: 1165 939 482
Fragments arranged by size:
1165 939 482
SspI AAT'ATT
Cuts at: 0 159 528 2234 2586
Size: 159 369 1706 352
Fragments arranged by size:
1706 369 352 159
StuI AGG'CCT
Cuts at: 0 1267 2586
Size: 1267 1319
StyI C'CwwG_G
Cuts at: 0 1184 2532 2586
Size: 1184 1348 54
Fragments arranged by size:
1348 1184 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2509 2557 2586
Size: 2509 48 29
Fragments arranged by size:
2509 48 29
TatI w'GTAC_w
Cuts at: 0 22 2586
Size: 22 2564
XhoI C'TCGA_G
Cuts at: 0 18 2586
Size: 18 2568
XmnI GAAnn'nnTTC
Cuts at: 0 846 1748 2210 2586
Size: 846 902 462 376
Fragments arranged by size:
902 846 462 376
ZraI GAC'GTC
Cuts at: 0 2346 2586
Size: 2346 240
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaWI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PshAI PsiI PspXI PstI PvuII
SacI SfcI SmlI SpeI SphI SspI StuI StyI
TaqII TatI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AloI ApaI AscI
AsiSI AvrII BamHI BbeI BglI BmtI BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmBI BspEI
BsrDI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I EagI
EciI EcoO109I EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PpuMI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SwaI TstI Tth111I XbaI XcmI XmaI