Restriction map for
pF1KB8501
(Linear) (Six-base) MAPSORT of: KIBB8501.seq Check: 698 from: 1 to: 2808
KIBB8501.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 367 1044 2485 2808
Size: 367 677 1441 323
Fragments arranged by size:
1441 677 367 323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1646 2808
Size: 916 730 1162
Fragments arranged by size:
1162 916 730
AflIII A'CryG_T
Cuts at: 0 2445 2808
Size: 2445 363
AhdI GACnn_n'nnGTC
Cuts at: 0 1848 2808
Size: 1848 960
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2099 2131 2808
Size: 2099 32 677
Fragments arranged by size:
2099 677 32
AlwNI CAG_nnn'CTG
Cuts at: 0 175 2808
Size: 175 2633
ApoI r'AATT_y
Cuts at: 0 294 605 657 690 781 859 870 1211
Size: 294 311 52 33 91 78 11 341
Cuts at: 1211 1257 1486 1666 1692 1915 2193 2612 2808
Size: 46 229 180 26 223 278 419 196
Fragments arranged by size:
419 341 311 294 278 229 223 196
180 91 78 52 46 33 26 11
AseI AT'TA_AT
Cuts at: 0 971 1406 2269 2808
Size: 971 435 863 539
Fragments arranged by size:
971 863 539 435
AvaI C'yCGr_G
Cuts at: 0 1601 2808
Size: 1601 1207
BamHI G'GATC_C
Cuts at: 0 1293 2712 2808
Size: 1293 1419 96
Fragments arranged by size:
1419 1293 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 1367 1564 2808
Size: 1367 197 1244
Fragments arranged by size:
1367 1244 197
BbvCI CC'TCA_GC
Cuts at: 0 4 2808
Size: 4 2804
BciVI GTATCCnnnnn_n'
Cuts at: 0 2420 2808
Size: 2420 388
BclI T'GATC_A
Cuts at: 0 590 680 1650 2027 2808
Size: 590 90 970 377 781
Fragments arranged by size:
970 781 590 377 90
BfrBI ATG'CAT
Cuts at: 0 773 2430 2808
Size: 773 1657 378
Fragments arranged by size:
1657 773 378
BglII A'GATC_T
Cuts at: 0 14 2808
Size: 14 2794
BmgBI CAC'GTC
Cuts at: 0 167 2808
Size: 167 2641
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 2808
Size: 552 2256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1055 2808
Size: 88 967 1753
Fragments arranged by size:
1753 967 88
Bpu10I CC'TnA_GC
Cuts at: 0 4 2808
Size: 4 2804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 2445 2731 2808
Size: 224 2221 286 77
Fragments arranged by size:
2221 286 224 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 147 2808
Size: 147 2661
BsaWI w'CCGG_w
Cuts at: 0 185 2808
Size: 185 2623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 1014 1044 2808
Size: 793 30 191 30 1764
Fragments arranged by size:
1764 793 191 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 434 1872 2808
Size: 308 126 1438 936
Fragments arranged by size:
1438 936 308 126
BsiEI CG_ry'CG
Cuts at: 0 186 2808
Size: 186 2622
BsrBI CCG'CTC
Cuts at: 0 71 2808
Size: 71 2737
BsrDI GCAATG_nn'
Cuts at: 0 1821 2808
Size: 1821 987
BssSI C'ACGA_G
Cuts at: 0 326 2808
Size: 326 2482
BstAPI GCAn_nnn'nTGC
Cuts at: 0 835 2808
Size: 835 1973
BstYI r'GATC_y
Cuts at: 0 14 1293 2712 2808
Size: 14 1279 1419 96
Fragments arranged by size:
1419 1279 96 14
BstZ17I GTA'TAC
Cuts at: 0 2486 2808
Size: 2486 322
Bsu36I CC'TnA_GG
Cuts at: 0 77 2808
Size: 77 2731
BtsI GCAGTG_nn'
Cuts at: 0 1472 2808
Size: 1472 1336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2664 2699 2808
Size: 2664 35 109
Fragments arranged by size:
2664 109 35
DraI TTT'AAA
Cuts at: 0 533 1301 2069 2808
Size: 533 768 768 739
Fragments arranged by size:
768 768 739 533
EaeI y'GGCC_r
Cuts at: 0 232 2808
Size: 232 2576
EarI CTCTTCn'nnn_
Cuts at: 0 2510 2808
Size: 2510 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 916 1055 1646 2808
Size: 88 828 139 591 1162
Fragments arranged by size:
1162 828 591 139 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 2808
Size: 799 2009
EcoO109I rG'GnC_Cy
Cuts at: 0 82 2808
Size: 82 2726
EcoRI G'AATT_C
Cuts at: 0 657 781 2808
Size: 657 124 2027
Fragments arranged by size:
2027 657 124
EcoRV GAT'ATC
Cuts at: 0 1547 2184 2808
Size: 1547 637 624
Fragments arranged by size:
1547 637 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 335 1141 2808
Size: 335 806 1667
Fragments arranged by size:
1667 806 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 793 825 928 960 1598 1630
Size: 4 32 757 32 103 32 638 32
Cuts at: 1630 1834 1866 2536 2568 2808
Size: 204 32 670 32 240
Fragments arranged by size:
757 670 638 240 204 103 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 1095 2808
Size: 1095 1713
HpaI GTT'AAC
Cuts at: 0 1095 2808
Size: 1095 1713
MfeI C'AATT_G
Cuts at: 0 2567 2707 2808
Size: 2567 140 101
Fragments arranged by size:
2567 140 101
MslI CAynn'nnrTG
Cuts at: 0 1618 2399 2808
Size: 1618 781 409
Fragments arranged by size:
1618 781 409
MspA1I CmG'CkG
Cuts at: 0 832 2808
Size: 832 1976
NdeI CA'TA_TG
Cuts at: 0 579 2112 2808
Size: 579 1533 696
Fragments arranged by size:
1533 696 579
NsiI A_TGCA'T
Cuts at: 0 775 2432 2808
Size: 775 1657 376
Fragments arranged by size:
1657 775 376
NspI r_CATG'y
Cuts at: 0 2430 2449 2808
Size: 2430 19 359
Fragments arranged by size:
2430 359 19
PacI TTA_AT'TAA
Cuts at: 0 971 1724 2808
Size: 971 753 1084
Fragments arranged by size:
1084 971 753
PciI A'CATG_T
Cuts at: 0 2445 2808
Size: 2445 363
PfoI T'CCnGG_A
Cuts at: 0 104 1032 2381 2808
Size: 104 928 1349 427
Fragments arranged by size:
1349 928 427 104
PpuMI rG'GwC_Cy
Cuts at: 0 82 2808
Size: 82 2726
PsiI TTA'TAA
Cuts at: 0 395 2808
Size: 395 2413
PspXI vC'TCGA_Gb
Cuts at: 0 1601 2808
Size: 1601 1207
PstI C_TGCA'G
Cuts at: 0 2544 2808
Size: 2544 264
PvuII CAG'CTG
Cuts at: 0 832 2808
Size: 832 1976
SanDI GG'GwC_CC
Cuts at: 0 82 2808
Size: 82 2726
ScaI AGT'ACT
Cuts at: 0 2172 2808
Size: 2172 636
SfcI C'TryA_G
Cuts at: 0 984 1625 2540 2808
Size: 984 641 915 268
Fragments arranged by size:
984 915 641 268
SmlI C'TyrA_G
Cuts at: 0 239 1601 2460 2746 2808
Size: 239 1362 859 286 62
Fragments arranged by size:
1362 859 286 239 62
SspI AAT'ATT
Cuts at: 0 2128 2808
Size: 2128 680
StuI AGG'CCT
Cuts at: 0 9 2808
Size: 9 2799
StyI C'CwwG_G
Cuts at: 0 1280 1355 2582 2808
Size: 1280 75 1227 226
Fragments arranged by size:
1280 1227 226 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 120 2808
Size: 120 2688
TatI w'GTAC_w
Cuts at: 0 755 2170 2756 2808
Size: 755 1415 586 52
Fragments arranged by size:
1415 755 586 52
Tth111I GACn'n_nGTC
Cuts at: 0 1228 2808
Size: 1228 1580
XhoI C'TCGA_G
Cuts at: 0 1601 2808
Size: 1601 1207
XmnI GAAnn'nnTTC
Cuts at: 0 730 863 1124 1817 2768 2786 2808
Size: 730 133 261 693 951 18 22
Fragments arranged by size:
951 730 693 261 133 22 18
Enzymes that do cut:
AccI AcuI AflIII AhdI AloI AlwNI ApoI AseI
AvaI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BsgI BsiEI BsrBI BsrDI BssSI BstAPI BstYI BstZ17I
Bsu36I BtsI CspCI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI MfeI
MslI MspA1I NdeI NsiI NspI PacI PciI PfoI
PpuMI PsiI PspXI PstI PvuII SanDI ScaI SfcI
SmlI SspI StuI StyI TaqII TatI Tth111I XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BanI
BanII BbeI BcgI BglI BlpI Bme1580I BmtI BplI
BsaAI BsaBI BsaHI BseRI BseYI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BssHII
BstBI BstEII BstXI BtgI BtgZI ClaI DraIII DrdI
EagI EciI EcoICRI FseI FspI FspAI HaeII HindIII
KasI KpnI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PasI PflMI PmeI PmlI
PpiI PshAI PspOMI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TstI XbaI
XcmI XmaI ZraI