Restriction map for
pF1KB8506
(Linear) (Six-base) MAPSORT of: KIBB8506.seq Check: 5719 from: 1 to: 2598
KIBB8506.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 0 851 2598
Size: 851 1747
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 670 680 1036 1121 1214 2598
Size: 595 75 10 356 85 93 1384
Fragments arranged by size:
1384 595 356 93 85 75 10
AflIII A'CryG_T
Cuts at: 0 120 130 2598
Size: 120 10 2468
Fragments arranged by size:
2468 120 10
AhdI GACnn_n'nnGTC
Cuts at: 0 858 2598
Size: 858 1740
AleI CACnn'nnGTG
Cuts at: 0 1222 1706 2598
Size: 1222 484 892
Fragments arranged by size:
1222 892 484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 38 2598
Size: 38 2560
AlwNI CAG_nnn'CTG
Cuts at: 0 190 411 1461 2478 2598
Size: 190 221 1050 1017 120
Fragments arranged by size:
1050 1017 221 190 120
ApaI G_GGCC'C
Cuts at: 0 2533 2598
Size: 2533 65
ApoI r'AATT_y
Cuts at: 0 1147 1792 2598
Size: 1147 645 806
Fragments arranged by size:
1147 806 645
AseI AT'TA_AT
Cuts at: 0 1487 2598
Size: 1487 1111
AvaI C'yCGr_G
Cuts at: 0 1168 1425 2598
Size: 1168 257 1173
Fragments arranged by size:
1173 1168 257
BamHI G'GATC_C
Cuts at: 0 493 636 2598
Size: 493 143 1962
Fragments arranged by size:
1962 493 143
BanII G_rGCy'C
Cuts at: 0 1676 2077 2533 2598
Size: 1676 401 456 65
Fragments arranged by size:
1676 456 401 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 349 2598
Size: 349 2249
BbvCI CC'TCA_GC
Cuts at: 0 1348 2598
Size: 1348 1250
BfrBI ATG'CAT
Cuts at: 0 2513 2598
Size: 2513 85
BglII A'GATC_T
Cuts at: 0 477 2598
Size: 477 2121
BlpI GC'TnA_GC
Cuts at: 0 1568 2598
Size: 1568 1030
Bme1580I G_kGCm'C
Cuts at: 0 1980 2533 2598
Size: 1980 553 65
Fragments arranged by size:
1980 553 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 396 1080 2598
Size: 210 186 684 1518
Fragments arranged by size:
1518 684 210 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1482 1639 2598
Size: 1482 157 959
Fragments arranged by size:
1482 959 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 473 1028 1231 1273 1660 2237 2598
Size: 187 286 555 203 42 387 577 361
Fragments arranged by size:
577 555 387 361 286 203 187 42
Bpu10I CC'TnA_GC
Cuts at: 0 1348 2598
Size: 1348 1250
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 866 2598
Size: 187 679 1732
Fragments arranged by size:
1732 679 187
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1346 2013 2034 2202 2598
Size: 1346 667 21 168 396
Fragments arranged by size:
1346 667 396 168 21
BseYI C'CCAG_C
Cuts at: 0 62 1654 2067 2598
Size: 62 1592 413 531
Fragments arranged by size:
1592 531 413 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 2598
Size: 1024 1574
BsiHKAI G_wGCw'C
Cuts at: 0 1368 1676 2433 2598
Size: 1368 308 757 165
Fragments arranged by size:
1368 757 308 165
BsmI GAATG_Cn'
Cuts at: 0 812 1828 2515 2598
Size: 812 1016 687 83
Fragments arranged by size:
1016 812 687 83
Bsp1286I G_dGCh'C
Cuts at: 0 1368 1676 1980 2077 2433 2533 2598
Size: 1368 308 304 97 356 100 65
Fragments arranged by size:
1368 356 308 304 100 97 65
BsrDI GCAATG_nn'
Cuts at: 0 482 2598
Size: 482 2116
BsrFI r'CCGG_y
Cuts at: 0 31 1799 2598
Size: 31 1768 799
Fragments arranged by size:
1768 799 31
BssSI C'ACGA_G
Cuts at: 0 1367 1438 2598
Size: 1367 71 1160
Fragments arranged by size:
1367 1160 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2089 2598
Size: 2089 509
BstYI r'GATC_y
Cuts at: 0 186 477 493 636 872 2416 2598
Size: 186 291 16 143 236 1544 182
Fragments arranged by size:
1544 291 236 186 182 143 16
Bsu36I CC'TnA_GG
Cuts at: 0 1447 2598
Size: 1447 1151
BtgI C'CryG_G
Cuts at: 0 1301 1846 2598
Size: 1301 545 752
Fragments arranged by size:
1301 752 545
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2228 2598
Size: 2228 370
BtsI GCAGTG_nn'
Cuts at: 0 1012 1217 1229 1400 2598
Size: 1012 205 12 171 1198
Fragments arranged by size:
1198 1012 205 171 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1074 1109 2598
Size: 1074 35 1489
Fragments arranged by size:
1489 1074 35
DraI TTT'AAA
Cuts at: 0 117 2598
Size: 117 2481
DraIII CAC_nnn'GTG
Cuts at: 0 1954 2598
Size: 1954 644
DrdI GACnn_nn'nnGTC
Cuts at: 0 506 2598
Size: 506 2092
EaeI y'GGCC_r
Cuts at: 0 743 1338 2598
Size: 743 595 1260
Fragments arranged by size:
1260 743 595
EarI CTCTTCn'nnn_
Cuts at: 0 41 755 761 1843 2220 2598
Size: 41 714 6 1082 377 378
Fragments arranged by size:
1082 714 378 377 41 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2025 2598
Size: 2025 573
EcoICRI GAG'CTC
Cuts at: 0 1674 2598
Size: 1674 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 473 595 670 680 1028 1036 1121
Size: 187 286 122 75 10 348 8 85
Cuts at: 1121 1214 1231 1273 1660 2237 2598
Size: 93 17 42 387 577 361
Fragments arranged by size:
577 387 361 348 286 187 122 93
85 75 42 17 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1615 2455 2598
Size: 1615 840 143
Fragments arranged by size:
1615 840 143
EcoO109I rG'GnC_Cy
Cuts at: 0 450 1443 1588 1620 2475 2530 2598
Size: 450 993 145 32 855 55 68
Fragments arranged by size:
993 855 450 145 68 55 32
EcoRI G'AATT_C
Cuts at: 0 1792 2598
Size: 1792 806
EcoRV GAT'ATC
Cuts at: 0 2237 2598
Size: 2237 361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1533 2598
Size: 1533 1065
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 171 203 364 396 844 876
Size: 19 32 120 32 161 32 448 32
Cuts at: 876 1675 1707 2598
Size: 799 32 891
Fragments arranged by size:
891 799 448 161 120 32 32 32
32 32 19
HindIII A'AGCT_T
Cuts at: 0 512 2598
Size: 512 2086
MscI TGG'CCA
Cuts at: 0 745 2598
Size: 745 1853
MslI CAynn'nnrTG
Cuts at: 0 1222 1706 2598
Size: 1222 484 892
Fragments arranged by size:
1222 892 484
MspA1I CmG'CkG
Cuts at: 0 441 1251 1680 2071 2598
Size: 441 810 429 391 527
Fragments arranged by size:
810 527 441 429 391
NaeI GCC'GGC
Cuts at: 0 33 1801 2598
Size: 33 1768 797
Fragments arranged by size:
1768 797 33
NcoI C'CATG_G
Cuts at: 0 1301 2598
Size: 1301 1297
NgoMIV G'CCGG_C
Cuts at: 0 31 1799 2598
Size: 31 1768 799
Fragments arranged by size:
1768 799 31
NsiI A_TGCA'T
Cuts at: 0 2515 2598
Size: 2515 83
NspI r_CATG'y
Cuts at: 0 124 425 2598
Size: 124 301 2173
Fragments arranged by size:
2173 301 124
PasI CC'CwG_GG
Cuts at: 0 2082 2598
Size: 2082 516
PciI A'CATG_T
Cuts at: 0 120 2598
Size: 120 2478
PflMI CCAn_nnn'nTGG
Cuts at: 0 323 1954 2598
Size: 323 1631 644
Fragments arranged by size:
1631 644 323
PfoI T'CCnGG_A
Cuts at: 0 489 518 2598
Size: 489 29 2080
Fragments arranged by size:
2080 489 29
PpuMI rG'GwC_Cy
Cuts at: 0 1588 1620 2598
Size: 1588 32 978
Fragments arranged by size:
1588 978 32
PshAI GACnn'nnGTC
Cuts at: 0 938 2598
Size: 938 1660
PspOMI G'GGCC_C
Cuts at: 0 2529 2598
Size: 2529 69
PspXI vC'TCGA_Gb
Cuts at: 0 1168 1425 2598
Size: 1168 257 1173
Fragments arranged by size:
1173 1168 257
PstI C_TGCA'G
Cuts at: 0 1224 2598
Size: 1224 1374
PvuII CAG'CTG
Cuts at: 0 1251 1680 2598
Size: 1251 429 918
Fragments arranged by size:
1251 918 429
SacI G_AGCT'C
Cuts at: 0 1676 2598
Size: 1676 922
SapI GCTCTTCn'nnn_
Cuts at: 0 761 2598
Size: 761 1837
ScaI AGT'ACT
Cuts at: 0 913 2598
Size: 913 1685
SexAI A'CCwGG_T
Cuts at: 0 1950 2598
Size: 1950 648
SfcI C'TryA_G
Cuts at: 0 548 848 884 1220 2173 2598
Size: 548 300 36 336 953 425
Fragments arranged by size:
953 548 425 336 300 36
SmlI C'TyrA_G
Cuts at: 0 1168 1425 2598
Size: 1168 257 1173
Fragments arranged by size:
1173 1168 257
SspI AAT'ATT
Cuts at: 0 1498 2447 2598
Size: 1498 949 151
Fragments arranged by size:
1498 949 151
StyI C'CwwG_G
Cuts at: 0 1301 2598
Size: 1301 1297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1831 2239 2598
Size: 311 1520 408 359
Fragments arranged by size:
1520 408 359 311
TatI w'GTAC_w
Cuts at: 0 911 1884 2149 2598
Size: 911 973 265 449
Fragments arranged by size:
973 911 449 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 84 116 2598
Size: 84 32 2482
Fragments arranged by size:
2482 84 32
XbaI T'CTAG_A
Cuts at: 0 75 2598
Size: 75 2523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2464 2598
Size: 2464 134
XhoI C'TCGA_G
Cuts at: 0 1168 1425 2598
Size: 1168 257 1173
Fragments arranged by size:
1173 1168 257
XmnI GAAnn'nnTTC
Cuts at: 0 2357 2598
Size: 2357 241
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AseI AvaI BamHI BanII BbsI BbvCI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SapI ScaI SexAI SfcI SmlI SspI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaLI AscI AsiSI AvrII BaeI BanI BbeI BcgI
BciVI BclI BglI BmgBI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
ClaI EagI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI PacI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XmaI ZraI