Restriction map for
pF1KB8507
(Linear) (Six-base) MAPSORT of: KIBB8507.seq Check: 6828 from: 1 to: 2823
KIBB8507.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 105 2584 2823
Size: 105 2479 239
Fragments arranged by size:
2479 239 105
AatII G_ACGT'C
Cuts at: 0 1985 2823
Size: 1985 838
AccI GT'mk_AC
Cuts at: 0 764 2823
Size: 764 2059
Acc65I G'GTAC_C
Cuts at: 0 1541 2384 2823
Size: 1541 843 439
Fragments arranged by size:
1541 843 439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1567 1711 1981 2823
Size: 298 1269 144 270 842
Fragments arranged by size:
1269 842 298 270 144
AfeI AGC'GCT
Cuts at: 0 1639 2823
Size: 1639 1184
AleI CACnn'nnGTG
Cuts at: 0 1832 2823
Size: 1832 991
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 721 753 2823
Size: 619 32 70 32 2070
Fragments arranged by size:
2070 619 70 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 732 1143 1326 1689 2277 2471 2823
Size: 732 411 183 363 588 194 352
Fragments arranged by size:
732 588 411 363 352 194 183
ApaI G_GGCC'C
Cuts at: 0 794 1905 2619 2823
Size: 794 1111 714 204
Fragments arranged by size:
1111 794 714 204
AvaI C'yCGr_G
Cuts at: 0 85 515 2823
Size: 85 430 2308
Fragments arranged by size:
2308 430 85
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 253 286 410 443 2823
Size: 253 33 124 33 2380
Fragments arranged by size:
2380 253 124 33 33
BamHI G'GATC_C
Cuts at: 0 106 1914 2823
Size: 106 1808 909
Fragments arranged by size:
1808 909 106
BanI G'GyrC_C
Cuts at: 0 1541 2384 2823
Size: 1541 843 439
Fragments arranged by size:
1541 843 439
BanII G_rGCy'C
Cuts at: 0 794 916 1015 1905 2429 2619 2823
Size: 794 122 99 890 524 190 204
Fragments arranged by size:
890 794 524 204 190 122 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 1568 2823
Size: 1568 1255
BbvCI CC'TCA_GC
Cuts at: 0 2170 2823
Size: 2170 653
BciVI GTATCCnnnnn_n'
Cuts at: 0 2416 2823
Size: 2416 407
BclI T'GATC_A
Cuts at: 0 2643 2823
Size: 2643 180
BglI GCCn_nnn'nGGC
Cuts at: 0 1727 2823
Size: 1727 1096
BglII A'GATC_T
Cuts at: 0 200 341 2634 2823
Size: 200 141 2293 189
Fragments arranged by size:
2293 200 189 141
BlpI GC'TnA_GC
Cuts at: 0 64 705 2823
Size: 64 641 2118
Fragments arranged by size:
2118 641 64
Bme1580I G_kGCm'C
Cuts at: 0 794 1905 2619 2821 2823
Size: 794 1111 714 202 2
Fragments arranged by size:
1111 794 714 202 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 1908 2823
Size: 1908 915
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 1207 2511 2565 2823
Size: 1029 178 1304 54 258
Fragments arranged by size:
1304 1029 258 178 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1102 1429 2751 2793 2794 2823
Size: 373 729 327 1322 42 1 29
Fragments arranged by size:
1322 729 373 327 42 29 1
Bpu10I CC'TnA_GC
Cuts at: 0 2170 2662 2823
Size: 2170 492 161
Fragments arranged by size:
2170 492 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1900 2209 2413 2823
Size: 1375 525 309 204 410
Fragments arranged by size:
1375 525 410 309 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 2389 2823
Size: 1178 1211 434
Fragments arranged by size:
1211 1178 434
BsaAI yAC'GTr
Cuts at: 0 1023 1266 2823
Size: 1023 243 1557
Fragments arranged by size:
1557 1023 243
BsaHI Gr'CG_yC
Cuts at: 0 1982 2823
Size: 1982 841
BsaWI w'CCGG_w
Cuts at: 0 866 2591 2823
Size: 866 1725 232
Fragments arranged by size:
1725 866 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1075 1105 2621 2651 2730 2760 2823
Size: 1075 30 1516 30 79 30 63
Fragments arranged by size:
1516 1075 79 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1360 1426 1786 2206 2407 2823
Size: 1360 66 360 420 201 416
Fragments arranged by size:
1360 420 416 360 201 66
BseYI C'CCAG_C
Cuts at: 0 1289 1717 2779 2823
Size: 1289 428 1062 44
Fragments arranged by size:
1289 1062 428 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1312 1861 2290 2484 2823
Size: 366 946 549 429 194 339
Fragments arranged by size:
946 549 429 366 339 194
BsiHKAI G_wGCw'C
Cuts at: 0 916 1829 2429 2671 2823
Size: 916 913 600 242 152
Fragments arranged by size:
916 913 600 242 152
BsmI GAATG_Cn'
Cuts at: 0 1295 2823
Size: 1295 1528
Bsp1286I G_dGCh'C
Cuts at: 0 794 916 1015 1829 1905 2429 2619 2671
Size: 794 122 99 814 76 524 190 52
Cuts at: 2671 2821 2823
Size: 150 2
Fragments arranged by size:
814 794 524 190 150 122 99 76
52 2
BspHI T'CATG_A
Cuts at: 0 1533 2823
Size: 1533 1290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 105 740 1325 2100 2584 2823
Size: 20 85 635 585 775 484 239
Fragments arranged by size:
775 635 585 484 239 85 20
BsrBI CCG'CTC
Cuts at: 0 82 2823
Size: 82 2741
BsrDI GCAATG_nn'
Cuts at: 0 75 821 2823
Size: 75 746 2002
Fragments arranged by size:
2002 746 75
BsrFI r'CCGG_y
Cuts at: 0 521 2105 2823
Size: 521 1584 718
Fragments arranged by size:
1584 718 521
BssSI C'ACGA_G
Cuts at: 0 1906 2095 2823
Size: 1906 189 728
Fragments arranged by size:
1906 728 189
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1326 2076 2823
Size: 1326 750 747
Fragments arranged by size:
1326 750 747
BstXI CCAn_nnnn'nTGG
Cuts at: 0 691 2823
Size: 691 2132
BstYI r'GATC_y
Cuts at: 0 106 200 341 1550 1649 1862 1914 2417
Size: 106 94 141 1209 99 213 52 503
Cuts at: 2417 2634 2823
Size: 217 189
Fragments arranged by size:
1209 503 217 213 189 141 106 99
94 52
BtgI C'CryG_G
Cuts at: 0 684 2133 2823
Size: 684 1449 690
Fragments arranged by size:
1449 690 684
BtsI GCAGTG_nn'
Cuts at: 0 22 1051 2532 2823
Size: 22 1029 1481 291
Fragments arranged by size:
1481 1029 291 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 2323 2823
Size: 2323 500
EaeI y'GGCC_r
Cuts at: 0 464 746 1343 2514 2823
Size: 464 282 597 1171 309
Fragments arranged by size:
1171 597 464 309 282
EarI CTCTTCn'nnn_
Cuts at: 0 311 352 1957 2264 2823
Size: 311 41 1605 307 559
Fragments arranged by size:
1605 559 311 307 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1531 2398 2823
Size: 1531 867 425
Fragments arranged by size:
1531 867 425
EcoICRI GAG'CTC
Cuts at: 0 914 2427 2823
Size: 914 1513 396
Fragments arranged by size:
1513 914 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 373 1102 1429 1567 1711 1981 2751
Size: 298 75 729 327 138 144 270 770
Cuts at: 2751 2793 2794 2823
Size: 42 1 29
Fragments arranged by size:
770 729 327 298 270 144 138 75
42 29 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 2823
Size: 1480 1343
EcoO109I rG'GnC_Cy
Cuts at: 0 729 791 1473 1746 1995 2145 2166 2823
Size: 729 62 682 273 249 150 21 657
Fragments arranged by size:
729 682 657 273 249 150 62 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 737 1951 2823
Size: 737 1214 872
Fragments arranged by size:
1214 872 737
HaeII r_GCGC'y
Cuts at: 0 27 1641 2133 2823
Size: 27 1614 492 690
Fragments arranged by size:
1614 690 492 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1157 2730 2762 2823
Size: 1125 32 1573 32 61
Fragments arranged by size:
1573 1125 61 32 32
HindIII A'AGCT_T
Cuts at: 0 1933 2823
Size: 1933 890
KpnI G_GTAC'C
Cuts at: 0 1545 2388 2823
Size: 1545 843 435
Fragments arranged by size:
1545 843 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 656 941 1232 2823
Size: 285 371 285 291 1591
Fragments arranged by size:
1591 371 291 285 285
MscI TGG'CCA
Cuts at: 0 466 1345 2516 2823
Size: 466 879 1171 307
Fragments arranged by size:
1171 879 466 307
MslI CAynn'nnrTG
Cuts at: 0 689 1451 1832 2119 2605 2823
Size: 689 762 381 287 486 218
Fragments arranged by size:
762 689 486 381 287 218
MspA1I CmG'CkG
Cuts at: 0 63 112 2218 2823
Size: 63 49 2106 605
Fragments arranged by size:
2106 605 63 49
NcoI C'CATG_G
Cuts at: 0 684 2133 2823
Size: 684 1449 690
Fragments arranged by size:
1449 690 684
NspI r_CATG'y
Cuts at: 0 2091 2823
Size: 2091 732
PasI CC'CwG_GG
Cuts at: 0 1857 2823
Size: 1857 966
PflMI CCAn_nnn'nTGG
Cuts at: 0 1143 2823
Size: 1143 1680
PfoI T'CCnGG_A
Cuts at: 0 405 2024 2809 2823
Size: 405 1619 785 14
Fragments arranged by size:
1619 785 405 14
PpuMI rG'GwC_Cy
Cuts at: 0 729 1473 2145 2823
Size: 729 744 672 678
Fragments arranged by size:
744 729 678 672
PspOMI G'GGCC_C
Cuts at: 0 790 1901 2615 2823
Size: 790 1111 714 208
Fragments arranged by size:
1111 790 714 208
PstI C_TGCA'G
Cuts at: 0 17 737 1058 1322 1759 1895 2823
Size: 17 720 321 264 437 136 928
Fragments arranged by size:
928 720 437 321 264 136 17
PvuII CAG'CTG
Cuts at: 0 2218 2823
Size: 2218 605
SacI G_AGCT'C
Cuts at: 0 916 2429 2823
Size: 916 1513 394
Fragments arranged by size:
1513 916 394
SexAI A'CCwGG_T
Cuts at: 0 2321 2823
Size: 2321 502
SfcI C'TryA_G
Cuts at: 0 13 223 733 966 1054 1194 1318 1755
Size: 13 210 510 233 88 140 124 437
Cuts at: 1755 1891 2335 2823
Size: 136 444 488
Fragments arranged by size:
510 488 444 437 233 210 140 136
124 88 13
SmaI CCC'GGG
Cuts at: 0 87 517 2823
Size: 87 430 2306
Fragments arranged by size:
2306 430 87
SmlI C'TyrA_G
Cuts at: 0 1390 1879 2224 2428 2823
Size: 1390 489 345 204 395
Fragments arranged by size:
1390 489 395 345 204
SspI AAT'ATT
Cuts at: 0 291 2823
Size: 291 2532
StyI C'CwwG_G
Cuts at: 0 332 684 2133 2483 2823
Size: 332 352 1449 350 340
Fragments arranged by size:
1449 352 350 340 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 2755 2823
Size: 697 2058 68
Fragments arranged by size:
2058 697 68
TatI w'GTAC_w
Cuts at: 0 1702 2823
Size: 1702 1121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 2823
Size: 1321 32 1470
Fragments arranged by size:
1470 1321 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1248 2509 2823
Size: 1248 1261 314
Fragments arranged by size:
1261 1248 314
XmaI C'CCGG_G
Cuts at: 0 85 515 2823
Size: 85 430 2308
Fragments arranged by size:
2308 430 85
XmnI GAAnn'nnTTC
Cuts at: 0 1586 1816 2062 2823
Size: 1586 230 246 761
Fragments arranged by size:
1586 761 246 230
ZraI GAC'GTC
Cuts at: 0 1983 2823
Size: 1983 840
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI AloI
AlwNI ApaI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SexAI SfcI SmaI
SmlI SspI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AlfI ApaLI ApoI
AscI AseI AsiSI AvrII BbeI BcgI BfrBI BmgBI
BmtI BsaBI BsiEI BsiWI BsmBI BspEI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XhoI