Restriction map for
pF1KB8508
(Linear) (Six-base) MAPSORT of: KIBB8508.seq Check: 3162 from: 1 to: 2598
KIBB8508.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
Acc65I G'GTAC_C
Cuts at: 0 729 1924 2598
Size: 729 1195 674
Fragments arranged by size:
1195 729 674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 442 601 706 1904 2264 2598
Size: 107 335 159 105 1198 360 334
Fragments arranged by size:
1198 360 335 334 159 107 105
AflIII A'CryG_T
Cuts at: 0 1369 2598
Size: 1369 1229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1065 1097 2598
Size: 1065 32 1501
Fragments arranged by size:
1501 1065 32
AlwNI CAG_nnn'CTG
Cuts at: 0 462 1198 1651 1892 2598
Size: 462 736 453 241 706
Fragments arranged by size:
736 706 462 453 241
ApoI r'AATT_y
Cuts at: 0 267 332 705 1705 2049 2116 2598
Size: 267 65 373 1000 344 67 482
Fragments arranged by size:
1000 482 373 344 267 67 65
AvaI C'yCGr_G
Cuts at: 0 809 1474 2598
Size: 809 665 1124
Fragments arranged by size:
1124 809 665
BamHI G'GATC_C
Cuts at: 0 1960 2598
Size: 1960 638
BanI G'GyrC_C
Cuts at: 0 729 1535 1924 2598
Size: 729 806 389 674
Fragments arranged by size:
806 729 674 389
BanII G_rGCy'C
Cuts at: 0 483 898 937 976 1015 1054 1132 1325
Size: 483 415 39 39 39 39 78 193
Cuts at: 1325 1645 2164 2476 2598
Size: 320 519 312 122
Fragments arranged by size:
519 483 415 320 312 193 122 78
39 39 39 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 2598
Size: 179 2419
BbvCI CC'TCA_GC
Cuts at: 0 354 2598
Size: 354 2244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 564 2598
Size: 530 34 2034
Fragments arranged by size:
2034 530 34
BglI GCCn_nnn'nGGC
Cuts at: 0 197 2598
Size: 197 2401
BglII A'GATC_T
Cuts at: 0 576 2598
Size: 576 2022
Bme1580I G_kGCm'C
Cuts at: 0 46 1115 1234 2598
Size: 46 1069 119 1364
Fragments arranged by size:
1364 1069 119 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 2199 2598
Size: 1236 963 399
Fragments arranged by size:
1236 963 399
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2349 2598
Size: 2349 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 965 2598
Size: 526 439 1633
Fragments arranged by size:
1633 526 439
Bpu10I CC'TnA_GC
Cuts at: 0 354 1324 2598
Size: 354 970 1274
Fragments arranged by size:
1274 970 354
BsaI GGTCTCn'nnnn_
Cuts at: 0 780 1990 2598
Size: 780 1210 608
Fragments arranged by size:
1210 780 608
BsaWI w'CCGG_w
Cuts at: 0 835 1069 2057 2598
Size: 835 234 988 541
Fragments arranged by size:
988 835 541 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 1604 1634 2598
Size: 83 30 1491 30 964
Fragments arranged by size:
1491 964 83 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 827 1787 2256 2598
Size: 107 720 960 469 342
Fragments arranged by size:
960 720 469 342 107
BseYI C'CCAG_C
Cuts at: 0 1108 2194 2598
Size: 1108 1086 404
Fragments arranged by size:
1108 1086 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2417 2598
Size: 2417 181
BsiHKAI G_wGCw'C
Cuts at: 0 483 898 937 976 1015 1132 1325 2598
Size: 483 415 39 39 39 117 193 1273
Fragments arranged by size:
1273 483 415 193 117 39 39 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 838 2598
Size: 838 1760
Bsp1286I G_dGCh'C
Cuts at: 0 46 483 898 937 976 1015 1054 1115
Size: 46 437 415 39 39 39 39 61
Cuts at: 1115 1132 1234 1325 1645 2164 2476 2598
Size: 17 102 91 320 519 312 122
Fragments arranged by size:
519 437 415 320 312 122 102 91
61 46 39 39 39 39 17
BspHI T'CATG_A
Cuts at: 0 1836 2598
Size: 1836 762
BsrDI GCAATG_nn'
Cuts at: 0 620 1487 2136 2598
Size: 620 867 649 462
Fragments arranged by size:
867 649 620 462
BssSI C'ACGA_G
Cuts at: 0 566 1758 2598
Size: 566 1192 840
Fragments arranged by size:
1192 840 566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 55 2598
Size: 55 2543
BstEII G'GTnAC_C
Cuts at: 0 1344 1446 2598
Size: 1344 102 1152
Fragments arranged by size:
1344 1152 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1045 1443 2598
Size: 1045 398 1155
Fragments arranged by size:
1155 1045 398
BstYI r'GATC_y
Cuts at: 0 396 527 576 1960 2598
Size: 396 131 49 1384 638
Fragments arranged by size:
1384 638 396 131 49
Bsu36I CC'TnA_GG
Cuts at: 0 2557 2598
Size: 2557 41
BtgI C'CryG_G
Cuts at: 0 1716 2598
Size: 1716 882
BtsI GCAGTG_nn'
Cuts at: 0 876 1562 2279 2598
Size: 876 686 717 319
Fragments arranged by size:
876 717 686 319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 98 133 2598
Size: 98 35 2465
Fragments arranged by size:
2465 98 35
EarI CTCTTCn'nnn_
Cuts at: 0 1235 2381 2598
Size: 1235 1146 217
Fragments arranged by size:
1235 1146 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1516 2598
Size: 1516 1082
EcoICRI GAG'CTC
Cuts at: 0 481 896 935 974 1013 1130 1323 2598
Size: 481 415 39 39 39 117 193 1275
Fragments arranged by size:
1275 481 415 193 117 39 39 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 442 526 601 706 965 1904 2264
Size: 107 335 84 75 105 259 939 360
Cuts at: 2264 2598
Size: 334
Fragments arranged by size:
939 360 335 334 259 107 105 84
75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1607 2364 2598
Size: 1607 757 234
Fragments arranged by size:
1607 757 234
EcoO109I rG'GnC_Cy
Cuts at: 0 2582 2598
Size: 2582 16
EcoRI G'AATT_C
Cuts at: 0 1705 2116 2598
Size: 1705 411 482
Fragments arranged by size:
1705 482 411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 652 2598
Size: 652 1946
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 1552 1584 1604 1618 1636 1650
Size: 337 32 1183 32 20 14 18 14
Cuts at: 1650 2598
Size: 948
Fragments arranged by size:
1183 948 337 32 32 20 18 14
14
HincII GTy'rAC
Cuts at: 0 314 2598
Size: 314 2284
HindIII A'AGCT_T
Cuts at: 0 2204 2598
Size: 2204 394
KpnI G_GTAC'C
Cuts at: 0 733 1928 2598
Size: 733 1195 670
Fragments arranged by size:
1195 733 670
MluI A'CGCG_T
Cuts at: 0 1369 2598
Size: 1369 1229
MslI CAynn'nnrTG
Cuts at: 0 1541 2598
Size: 1541 1057
NspI r_CATG'y
Cuts at: 0 762 2349 2598
Size: 762 1587 249
Fragments arranged by size:
1587 762 249
PasI CC'CwG_GG
Cuts at: 0 928 1045 1693 2467 2598
Size: 928 117 648 774 131
Fragments arranged by size:
928 774 648 131 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 2598
Size: 1198 1400
PfoI T'CCnGG_A
Cuts at: 0 699 2598
Size: 699 1899
PspXI vC'TCGA_Gb
Cuts at: 0 809 2598
Size: 809 1789
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 2598
Size: 1011 32 1555
Fragments arranged by size:
1555 1011 32
PstI C_TGCA'G
Cuts at: 0 60 205 467 2598
Size: 60 145 262 2131
Fragments arranged by size:
2131 262 145 60
SacI G_AGCT'C
Cuts at: 0 483 898 937 976 1015 1132 1325 2598
Size: 483 415 39 39 39 117 193 1273
Fragments arranged by size:
1273 483 415 193 117 39 39 39
ScaI AGT'ACT
Cuts at: 0 912 990 1029 1146 2598
Size: 912 78 39 117 1452
Fragments arranged by size:
1452 912 117 78 39
SexAI A'CCwGG_T
Cuts at: 0 789 1059 2598
Size: 789 270 1539
Fragments arranged by size:
1539 789 270
SfcI C'TryA_G
Cuts at: 0 56 201 463 2598
Size: 56 145 262 2135
Fragments arranged by size:
2135 262 145 56
SmlI C'TyrA_G
Cuts at: 0 809 2598
Size: 809 1789
SpeI A'CTAG_T
Cuts at: 0 1750 2598
Size: 1750 848
StuI AGG'CCT
Cuts at: 0 1212 2598
Size: 1212 1386
StyI C'CwwG_G
Cuts at: 0 109 2485 2598
Size: 109 2376 113
Fragments arranged by size:
2376 113 109
TatI w'GTAC_w
Cuts at: 0 444 910 988 1027 1144 2327 2598
Size: 444 466 78 39 117 1183 271
Fragments arranged by size:
1183 466 444 271 117 78 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 83 115 2598
Size: 83 32 2483
Fragments arranged by size:
2483 83 32
Tth111I GACn'n_nGTC
Cuts at: 0 1632 2598
Size: 1632 966
XbaI T'CTAG_A
Cuts at: 0 643 2598
Size: 643 1955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 2598
Size: 324 2274
XhoI C'TCGA_G
Cuts at: 0 809 2598
Size: 809 1789
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII HindIII KpnI MluI MslI NspI PasI
PflMI PfoI PspXI PsrI PstI SacI ScaI SexAI
SfcI SmlI SpeI StuI StyI TatI TstI Tth111I
XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BclI BfrBI BlpI BmgBI BmtI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI
ClaI DraI DraIII DrdI EaeI EagI EcoRV FseI
FspI FspAI HaeII HpaI KasI MfeI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI TaqII XmaI XmnI
ZraI