Restriction map for
pF1KB8511
(Linear) (Six-base) MAPSORT of: KIBB8511.seq Check: 9997 from: 1 to: 2823
KIBB8511.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1570 1692 2823
Size: 1570 122 1131
Fragments arranged by size:
1570 1131 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 2332 2395 2521 2823
Size: 1268 1064 63 126 302
Fragments arranged by size:
1268 1064 302 126 63
AfeI AGC'GCT
Cuts at: 0 2698 2823
Size: 2698 125
AhdI GACnn_n'nnGTC
Cuts at: 0 796 2823
Size: 796 2027
AleI CACnn'nnGTG
Cuts at: 0 1297 2232 2823
Size: 1297 935 591
Fragments arranged by size:
1297 935 591
ApaI G_GGCC'C
Cuts at: 0 107 1983 2823
Size: 107 1876 840
Fragments arranged by size:
1876 840 107
ApaLI G'TGCA_C
Cuts at: 0 1673 2823
Size: 1673 1150
ApoI r'AATT_y
Cuts at: 0 831 2823
Size: 831 1992
AvaI C'yCGr_G
Cuts at: 0 481 1189 1766 2024 2279 2458 2823
Size: 481 708 577 258 255 179 365
Fragments arranged by size:
708 577 481 365 258 255 179
AvrII C'CTAG_G
Cuts at: 0 24 2823
Size: 24 2799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2415 2448 2823
Size: 2415 33 375
Fragments arranged by size:
2415 375 33
BamHI G'GATC_C
Cuts at: 0 600 1517 2823
Size: 600 917 1306
Fragments arranged by size:
1306 917 600
BanI G'GyrC_C
Cuts at: 0 70 1840 2823
Size: 70 1770 983
Fragments arranged by size:
1770 983 70
BanII G_rGCy'C
Cuts at: 0 107 1307 1983 2823
Size: 107 1200 676 840
Fragments arranged by size:
1200 840 676 107
BbeI G_GCGC'C
Cuts at: 0 74 1844 2823
Size: 74 1770 979
Fragments arranged by size:
1770 979 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2260 2294 2823
Size: 2260 34 529
Fragments arranged by size:
2260 529 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2443 2823
Size: 2443 380
BglI GCCn_nnn'nGGC
Cuts at: 0 1906 2823
Size: 1906 917
BglII A'GATC_T
Cuts at: 0 1928 2085 2823
Size: 1928 157 738
Fragments arranged by size:
1928 738 157
BlpI GC'TnA_GC
Cuts at: 0 472 763 1267 1412 2823
Size: 472 291 504 145 1411
Fragments arranged by size:
1411 504 472 291 145
Bme1580I G_kGCm'C
Cuts at: 0 107 1018 1677 1983 2016 2422 2823
Size: 107 911 659 306 33 406 401
Fragments arranged by size:
911 659 406 401 306 107 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 974 2823
Size: 974 1849
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 761 2823
Size: 761 2062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 1438 1687 1972 2071 2275 2377 2823
Size: 1348 90 249 285 99 204 102 446
Fragments arranged by size:
1348 446 285 249 204 102 99 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 418 877 2823
Size: 252 166 459 1946
Fragments arranged by size:
1946 459 252 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 1324 2823
Size: 1324 1499
BsaAI yAC'GTr
Cuts at: 0 51 2823
Size: 51 2772
BsaBI GATnn'nnATC
Cuts at: 0 1126 2823
Size: 1126 1697
BsaHI Gr'CG_yC
Cuts at: 0 71 679 1841 2823
Size: 71 608 1162 982
Fragments arranged by size:
1162 982 608 71
BsaWI w'CCGG_w
Cuts at: 0 577 2428 2823
Size: 577 1851 395
Fragments arranged by size:
1851 577 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2719 2749 2823
Size: 2719 30 74
Fragments arranged by size:
2719 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 869 880 1618 1939 2110 2170 2272 2490
Size: 869 11 738 321 171 60 102 218
Cuts at: 2490 2686 2698 2823
Size: 196 12 125
Fragments arranged by size:
869 738 321 218 196 171 125 102
60 12 11
BseYI C'CCAG_C
Cuts at: 0 60 253 1546 1711 2823
Size: 60 193 1293 165 1112
Fragments arranged by size:
1293 1112 193 165 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2729 2823
Size: 2729 94
BsiEI CG_ry'CG
Cuts at: 0 1464 2805 2823
Size: 1464 1341 18
Fragments arranged by size:
1464 1341 18
BsiHKAI G_wGCw'C
Cuts at: 0 1307 1677 2286 2531 2823
Size: 1307 370 609 245 292
Fragments arranged by size:
1307 609 370 292 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 901 2823
Size: 901 1922
Bsp1286I G_dGCh'C
Cuts at: 0 107 1018 1307 1677 1983 2016 2286 2422
Size: 107 911 289 370 306 33 270 136
Cuts at: 2422 2531 2823
Size: 109 292
Fragments arranged by size:
911 370 306 292 289 270 136 109
107 33
BspHI T'CATG_A
Cuts at: 0 995 2033 2823
Size: 995 1038 790
Fragments arranged by size:
1038 995 790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1570 1692 2823
Size: 1570 122 1131
Fragments arranged by size:
1570 1131 122
BsrBI CCG'CTC
Cuts at: 0 1977 2823
Size: 1977 846
BsrFI r'CCGG_y
Cuts at: 0 2800 2823
Size: 2800 23
BssHII G'CGCG_C
Cuts at: 0 1734 2823
Size: 1734 1089
BssSI C'ACGA_G
Cuts at: 0 1676 2823
Size: 1676 1147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1080 1299 1494 1857 2490 2823
Size: 1080 219 195 363 633 333
Fragments arranged by size:
1080 633 363 333 219 195
BstYI r'GATC_y
Cuts at: 0 213 600 1517 1928 2085 2823
Size: 213 387 917 411 157 738
Fragments arranged by size:
917 738 411 387 213 157
Bsu36I CC'TnA_GG
Cuts at: 0 2788 2823
Size: 2788 35
BtgI C'CryG_G
Cuts at: 0 1079 1758 2537 2823
Size: 1079 679 779 286
Fragments arranged by size:
1079 779 679 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 506 2367 2739 2823
Size: 506 1861 372 84
Fragments arranged by size:
1861 506 372 84
BtsI GCAGTG_nn'
Cuts at: 0 1519 1806 2016 2823
Size: 1519 287 210 807
Fragments arranged by size:
1519 807 287 210
DraI TTT'AAA
Cuts at: 0 830 2823
Size: 830 1993
EaeI y'GGCC_r
Cuts at: 0 729 1556 1994 2399 2534 2540 2798 2802
Size: 729 827 438 405 135 6 258 4
Cuts at: 2802 2823
Size: 21
Fragments arranged by size:
827 729 438 405 258 135 21 6
4
EagI C'GGCC_G
Cuts at: 0 2802 2823
Size: 2802 21
EarI CTCTTCn'nnn_
Cuts at: 0 268 458 1714 2823
Size: 268 190 1256 1109
Fragments arranged by size:
1256 1109 268 190
EcoICRI GAG'CTC
Cuts at: 0 1305 2823
Size: 1305 1518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1348 1438 1687 1972 2071 2275 2332
Size: 1268 80 90 249 285 99 204 57
Cuts at: 2332 2377 2395 2521 2823
Size: 45 18 126 302
Fragments arranged by size:
1268 302 285 249 204 126 99 90
80 57 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 701 2823
Size: 701 2122
EcoO109I rG'GnC_Cy
Cuts at: 0 2100 2468 2823
Size: 2100 368 355
Fragments arranged by size:
2100 368 355
EcoRV GAT'ATC
Cuts at: 0 870 2823
Size: 870 1953
FseI GG_CCGG'CC
Cuts at: 0 2804 2823
Size: 2804 19
HaeII r_GCGC'y
Cuts at: 0 74 1844 2410 2700 2823
Size: 74 1770 566 290 123
Fragments arranged by size:
1770 566 290 123 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 782 814 845 875 877 900 907 932
Size: 782 32 31 30 2 23 7 25
Cuts at: 932 2823
Size: 1891
Fragments arranged by size:
1891 782 32 31 30 25 23 7
2
HincII GTy'rAC
Cuts at: 0 804 2823
Size: 804 2019
KasI G'GCGC_C
Cuts at: 0 70 1840 2823
Size: 70 1770 983
Fragments arranged by size:
1770 983 70
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 2321 2823
Size: 438 1883 502
Fragments arranged by size:
1883 502 438
MscI TGG'CCA
Cuts at: 0 731 1558 1996 2401 2542 2823
Size: 731 827 438 405 141 281
Fragments arranged by size:
827 731 438 405 281 141
MslI CAynn'nnrTG
Cuts at: 0 748 1297 1441 2232 2823
Size: 748 549 144 791 591
Fragments arranged by size:
791 748 591 549 144
MspA1I CmG'CkG
Cuts at: 0 513 2562 2823
Size: 513 2049 261
Fragments arranged by size:
2049 513 261
NaeI GCC'GGC
Cuts at: 0 2802 2823
Size: 2802 21
NarI GG'CG_CC
Cuts at: 0 71 1841 2823
Size: 71 1770 982
Fragments arranged by size:
1770 982 71
NcoI C'CATG_G
Cuts at: 0 1758 2823
Size: 1758 1065
NgoMIV G'CCGG_C
Cuts at: 0 2800 2823
Size: 2800 23
NspI r_CATG'y
Cuts at: 0 1198 2823
Size: 1198 1625
PasI CC'CwG_GG
Cuts at: 0 2329 2823
Size: 2329 494
PflMI CCAn_nnn'nTGG
Cuts at: 0 2132 2585 2823
Size: 2132 453 238
Fragments arranged by size:
2132 453 238
PfoI T'CCnGG_A
Cuts at: 0 1520 2823
Size: 1520 1303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 2823
Size: 577 32 2214
Fragments arranged by size:
2214 577 32
PspOMI G'GGCC_C
Cuts at: 0 103 1979 2823
Size: 103 1876 844
Fragments arranged by size:
1876 844 103
PspXI vC'TCGA_Gb
Cuts at: 0 1189 2279 2458 2823
Size: 1189 1090 179 365
Fragments arranged by size:
1189 1090 365 179
PstI C_TGCA'G
Cuts at: 0 1703 1793 1878 2414 2660 2823
Size: 1703 90 85 536 246 163
Fragments arranged by size:
1703 536 246 163 90 85
SacI G_AGCT'C
Cuts at: 0 1307 2823
Size: 1307 1516
SbfI CC_TGCA'GG
Cuts at: 0 1703 2823
Size: 1703 1120
SfcI C'TryA_G
Cuts at: 0 1382 1699 1789 1874 2410 2656 2823
Size: 1382 317 90 85 536 246 167
Fragments arranged by size:
1382 536 317 246 167 90 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1906 2823
Size: 1906 917
SfoI GGC'GCC
Cuts at: 0 72 1842 2823
Size: 72 1770 981
Fragments arranged by size:
1770 981 72
SmaI CCC'GGG
Cuts at: 0 2026 2823
Size: 2026 797
SmlI C'TyrA_G
Cuts at: 0 267 433 892 1189 2279 2458 2823
Size: 267 166 459 297 1090 179 365
Fragments arranged by size:
1090 459 365 297 267 179 166
StyI C'CwwG_G
Cuts at: 0 24 1073 1758 1997 2402 2573 2747 2823
Size: 24 1049 685 239 405 171 174 76
Fragments arranged by size:
1049 685 405 239 174 171 76 24
SwaI ATTT'AAAT
Cuts at: 0 830 2823
Size: 830 1993
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1403 1478 2046 2823
Size: 1403 75 568 777
Fragments arranged by size:
1403 777 568 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 624 656 2685 2717 2719 2751 2823
Size: 624 32 2029 32 2 32 72
Fragments arranged by size:
2029 624 72 32 32 32 2
XhoI C'TCGA_G
Cuts at: 0 1189 2279 2458 2823
Size: 1189 1090 179 365
Fragments arranged by size:
1189 1090 365 179
XmaI C'CCGG_G
Cuts at: 0 2024 2823
Size: 2024 799
XmnI GAAnn'nnTTC
Cuts at: 0 19 2818 2823
Size: 19 2799 5
Fragments arranged by size:
2799 19 5
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI ApaI ApaLI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BssSI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FseI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpiI PspOMI PspXI PstI
SacI SbfI SfcI SfiI SfoI SmaI SmlI StyI
SwaI TaqII TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AlfI
AloI AlwNI AscI AseI AsiSI BbsI BbvCI BclI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BspEI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII
DrdI EciI EcoRI FalI FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpuMI PshAI PsiI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI TatI Tth111I XbaI XcmI ZraI