Restriction map for
pF1KB8518
(Linear) (Six-base) MAPSORT of: KIBB8518.seq Check: 3694 from: 1 to: 2607
KIBB8518.orf
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2112 2607
Size: 2112 495
AatII G_ACGT'C
Cuts at: 0 1402 2607
Size: 1402 1205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 2607
Size: 36 2571
AleI CACnn'nnGTG
Cuts at: 0 1813 2607
Size: 1813 794
AlwNI CAG_nnn'CTG
Cuts at: 0 1108 2189 2347 2607
Size: 1108 1081 158 260
Fragments arranged by size:
1108 1081 260 158
ApaI G_GGCC'C
Cuts at: 0 2081 2607
Size: 2081 526
ApoI r'AATT_y
Cuts at: 0 194 489 1894 2561 2607
Size: 194 295 1405 667 46
Fragments arranged by size:
1405 667 295 194 46
AvaI C'yCGr_G
Cuts at: 0 586 2607
Size: 586 2021
BamHI G'GATC_C
Cuts at: 0 638 2607
Size: 638 1969
BanI G'GyrC_C
Cuts at: 0 1779 2607
Size: 1779 828
BanII G_rGCy'C
Cuts at: 0 11 342 651 1090 1211 1628 2061 2081
Size: 11 331 309 439 121 417 433 20
Cuts at: 2081 2607
Size: 526
Fragments arranged by size:
526 439 433 417 331 309 121 20
11
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 1252 2607
Size: 275 977 1355
Fragments arranged by size:
1355 977 275
BbvCI CC'TCA_GC
Cuts at: 0 1109 2607
Size: 1109 1498
BciVI GTATCCnnnnn_n'
Cuts at: 0 1718 2607
Size: 1718 889
BfrBI ATG'CAT
Cuts at: 0 845 1278 1320 2607
Size: 845 433 42 1287
Fragments arranged by size:
1287 845 433 42
BglI GCCn_nnn'nGGC
Cuts at: 0 1888 2198 2607
Size: 1888 310 409
Fragments arranged by size:
1888 409 310
BglII A'GATC_T
Cuts at: 0 2381 2607
Size: 2381 226
BlpI GC'TnA_GC
Cuts at: 0 1696 2099 2607
Size: 1696 403 508
Fragments arranged by size:
1696 508 403
Bme1580I G_kGCm'C
Cuts at: 0 1782 2081 2607
Size: 1782 299 526
Fragments arranged by size:
1782 526 299
BmrI ACTGGGnnnn_n'
Cuts at: 0 948 1084 2534 2607
Size: 948 136 1450 73
Fragments arranged by size:
1450 948 136 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2403 2607
Size: 2403 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 1723 2052 2223 2607
Size: 1172 551 329 171 384
Fragments arranged by size:
1172 551 384 329 171
Bpu10I CC'TnA_GC
Cuts at: 0 1109 2060 2607
Size: 1109 951 547
Fragments arranged by size:
1109 951 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 786 2607
Size: 543 243 1821
Fragments arranged by size:
1821 543 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 2070 2607
Size: 2070 537
BsaAI yAC'GTr
Cuts at: 0 2442 2607
Size: 2442 165
BsaHI Gr'CG_yC
Cuts at: 0 1399 2607
Size: 1399 1208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 360 390 2446 2476 2607
Size: 308 30 22 30 2056 30 131
Fragments arranged by size:
2056 308 131 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 238 332 1048 1431 1434 2440 2607
Size: 86 152 94 716 383 3 1006 167
Fragments arranged by size:
1006 716 383 167 152 94 86 3
BseYI C'CCAG_C
Cuts at: 0 164 1312 2607
Size: 164 1148 1295
Fragments arranged by size:
1295 1148 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 279 676 1164 2046 2607
Size: 167 112 397 488 882 561
Fragments arranged by size:
882 561 488 397 167 112
BsiHKAI G_wGCw'C
Cuts at: 0 11 1211 1628 2607
Size: 11 1200 417 979
Fragments arranged by size:
1200 979 417 11
BsmI GAATG_Cn'
Cuts at: 0 710 1303 2607
Size: 710 593 1304
Fragments arranged by size:
1304 710 593
Bsp1286I G_dGCh'C
Cuts at: 0 11 342 651 1090 1211 1628 1782 2061
Size: 11 331 309 439 121 417 154 279
Cuts at: 2061 2081 2607
Size: 20 526
Fragments arranged by size:
526 439 417 331 309 279 154 121
20 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2112 2607
Size: 2112 495
BsrBI CCG'CTC
Cuts at: 0 1674 2607
Size: 1674 933
BsrDI GCAATG_nn'
Cuts at: 0 2111 2607
Size: 2111 496
BsrFI r'CCGG_y
Cuts at: 0 1097 2607
Size: 1097 1510
BsrGI T'GTAC_A
Cuts at: 0 697 2492 2607
Size: 697 1795 115
Fragments arranged by size:
1795 697 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 290 994 2607
Size: 290 704 1613
Fragments arranged by size:
1613 704 290
BstEII G'GTnAC_C
Cuts at: 0 1981 2607
Size: 1981 626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2124 2607
Size: 2124 483
BstYI r'GATC_y
Cuts at: 0 638 1267 2381 2607
Size: 638 629 1114 226
Fragments arranged by size:
1114 638 629 226
Bsu36I CC'TnA_GG
Cuts at: 0 1031 2607
Size: 1031 1576
BtgI C'CryG_G
Cuts at: 0 1331 2483 2607
Size: 1331 1152 124
Fragments arranged by size:
1331 1152 124
BtsI GCAGTG_nn'
Cuts at: 0 155 1612 2607
Size: 155 1457 995
Fragments arranged by size:
1457 995 155
ClaI AT'CG_AT
Cuts at: 0 564 2607
Size: 564 2043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 81 116 2607
Size: 81 35 2491
Fragments arranged by size:
2491 81 35
DraIII CAC_nnn'GTG
Cuts at: 0 2022 2024 2607
Size: 2022 2 583
Fragments arranged by size:
2022 583 2
EaeI y'GGCC_r
Cuts at: 0 299 1099 2607
Size: 299 800 1508
Fragments arranged by size:
1508 800 299
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1235 1452 2607
Size: 1216 19 217 1155
Fragments arranged by size:
1216 1155 217 19
EcoICRI GAG'CTC
Cuts at: 0 9 1209 1626 2607
Size: 9 1200 417 981
Fragments arranged by size:
1200 981 417 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1172 1723 2052 2223 2607
Size: 36 1136 551 329 171 384
Fragments arranged by size:
1136 551 384 329 171 36
EcoO109I rG'GnC_Cy
Cuts at: 0 1326 2078 2607
Size: 1326 752 529
Fragments arranged by size:
1326 752 529
EcoRI G'AATT_C
Cuts at: 0 2561 2607
Size: 2561 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1015 1227 2607
Size: 1015 212 1380
Fragments arranged by size:
1380 1015 212
FspI TGC'GCA
Cuts at: 0 1218 2607
Size: 1218 1389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 2607
Size: 28 2579
HincII GTy'rAC
Cuts at: 0 15 2607
Size: 15 2592
HindIII A'AGCT_T
Cuts at: 0 1306 2607
Size: 1306 1301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2281 2494 2607
Size: 2281 213 113
Fragments arranged by size:
2281 213 113
MslI CAynn'nnrTG
Cuts at: 0 385 1813 2212 2607
Size: 385 1428 399 395
Fragments arranged by size:
1428 399 395 385
MspA1I CmG'CkG
Cuts at: 0 56 813 1105 2131 2607
Size: 56 757 292 1026 476
Fragments arranged by size:
1026 757 476 292 56
NaeI GCC'GGC
Cuts at: 0 1099 2607
Size: 1099 1508
NgoMIV G'CCGG_C
Cuts at: 0 1097 2607
Size: 1097 1510
NsiI A_TGCA'T
Cuts at: 0 847 1280 1322 2607
Size: 847 433 42 1285
Fragments arranged by size:
1285 847 433 42
NspI r_CATG'y
Cuts at: 0 845 1320 2010 2577 2607
Size: 845 475 690 567 30
Fragments arranged by size:
845 690 567 475 30
PasI CC'CwG_GG
Cuts at: 0 2074 2607
Size: 2074 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 731 2607
Size: 731 1876
PfoI T'CCnGG_A
Cuts at: 0 178 230 1136 1149 2239 2607
Size: 178 52 906 13 1090 368
Fragments arranged by size:
1090 906 368 178 52 13
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2236 2268 2607
Size: 2236 32 339
Fragments arranged by size:
2236 339 32
PpuMI rG'GwC_Cy
Cuts at: 0 1326 2607
Size: 1326 1281
PsiI TTA'TAA
Cuts at: 0 1364 2327 2607
Size: 1364 963 280
Fragments arranged by size:
1364 963 280
PspOMI G'GGCC_C
Cuts at: 0 2077 2607
Size: 2077 530
PstI C_TGCA'G
Cuts at: 0 812 894 2402 2532 2607
Size: 812 82 1508 130 75
Fragments arranged by size:
1508 812 130 82 75
PvuII CAG'CTG
Cuts at: 0 813 1105 2131 2607
Size: 813 292 1026 476
Fragments arranged by size:
1026 813 476 292
SacI G_AGCT'C
Cuts at: 0 11 1211 1628 2607
Size: 11 1200 417 979
Fragments arranged by size:
1200 979 417 11
SanDI GG'GwC_CC
Cuts at: 0 1326 2607
Size: 1326 1281
SapI GCTCTTCn'nnn_
Cuts at: 0 1216 2607
Size: 1216 1391
ScaI AGT'ACT
Cuts at: 0 879 1189 2607
Size: 879 310 1418
Fragments arranged by size:
1418 879 310
SfcI C'TryA_G
Cuts at: 0 808 890 1263 2339 2398 2528 2607
Size: 808 82 373 1076 59 130 79
Fragments arranged by size:
1076 808 373 130 82 79 59
SmlI C'TyrA_G
Cuts at: 0 522 801 2607
Size: 522 279 1806
Fragments arranged by size:
1806 522 279
SphI G_CATG'C
Cuts at: 0 1320 2607
Size: 1320 1287
SspI AAT'ATT
Cuts at: 0 1517 2607
Size: 1517 1090
StyI C'CwwG_G
Cuts at: 0 884 1711 2607
Size: 884 827 896
Fragments arranged by size:
896 884 827
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 803 2607
Size: 803 1804
TatI w'GTAC_w
Cuts at: 0 424 697 877 956 1187 2492 2607
Size: 424 273 180 79 231 1305 115
Fragments arranged by size:
1305 424 273 231 180 115 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 2560 2592 2607
Size: 308 32 2220 32 15
Fragments arranged by size:
2220 308 32 32 15
XbaI T'CTAG_A
Cuts at: 0 1356 2607
Size: 1356 1251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 309 455 2190 2607
Size: 261 48 146 1735 417
Fragments arranged by size:
1735 417 261 146 48
ZraI GAC'GTC
Cuts at: 0 1400 2607
Size: 1400 1207
Enzymes that do cut:
AarI AatII AcuI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BciVI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI Hin4I HincII HindIII MmeI MslI MspA1I NaeI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PsiI PspOMI PstI PvuII SacI SanDI SapI ScaI
SfcI SmlI SphI SspI StyI TaqII TatI TstI
XbaI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BclI BmgBI BmtI BsaBI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI BstZ17I
BtgZI DraI DrdI EagI EciI EcoNI EcoRV FseI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
NarI NcoI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PshAI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI
XmnI