Restriction map for
pF1KB8525
(Linear) (Six-base) MAPSORT of: KIBB8525.seq Check: 2491 from: 1 to: 2883
KIBB8525.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 0 2255 2883
Size: 2255 628
Acc65I G'GTAC_C
Cuts at: 0 125 2883
Size: 125 2758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1127 1609 2883
Size: 1081 46 482 1274
Fragments arranged by size:
1274 1081 482 46
AflIII A'CryG_T
Cuts at: 0 1851 2883
Size: 1851 1032
AleI CACnn'nnGTG
Cuts at: 0 754 2883
Size: 754 2129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2460 2883
Size: 2460 423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 2883
Size: 1531 32 1320
Fragments arranged by size:
1531 1320 32
AlwNI CAG_nnn'CTG
Cuts at: 0 284 1734 2883
Size: 284 1450 1149
Fragments arranged by size:
1450 1149 284
ApaI G_GGCC'C
Cuts at: 0 227 2883
Size: 227 2656
ApoI r'AATT_y
Cuts at: 0 1333 2883
Size: 1333 1550
AvaI C'yCGr_G
Cuts at: 0 42 140 374 2585 2883
Size: 42 98 234 2211 298
Fragments arranged by size:
2211 298 234 98 42
BanI G'GyrC_C
Cuts at: 0 21 125 1398 2774 2883
Size: 21 104 1273 1376 109
Fragments arranged by size:
1376 1273 109 104 21
BanII G_rGCy'C
Cuts at: 0 227 375 632 1946 2834 2883
Size: 227 148 257 1314 888 49
Fragments arranged by size:
1314 888 257 227 148 49
BbeI G_GCGC'C
Cuts at: 0 25 2778 2883
Size: 25 2753 105
Fragments arranged by size:
2753 105 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 945 1923 2064 2113 2629 2883
Size: 945 978 141 49 516 254
Fragments arranged by size:
978 945 516 254 141 49
BbvCI CC'TCA_GC
Cuts at: 0 761 2883
Size: 761 2122
BclI T'GATC_A
Cuts at: 0 2400 2579 2883
Size: 2400 179 304
Fragments arranged by size:
2400 304 179
BglI GCCn_nnn'nGGC
Cuts at: 0 44 1423 2883
Size: 44 1379 1460
Fragments arranged by size:
1460 1379 44
BglII A'GATC_T
Cuts at: 0 1205 2883
Size: 1205 1678
BlpI GC'TnA_GC
Cuts at: 0 2845 2883
Size: 2845 38
Bme1580I G_kGCm'C
Cuts at: 0 227 522 1049 2564 2883
Size: 227 295 527 1515 319
Fragments arranged by size:
1515 527 319 295 227
BmgBI CAC'GTC
Cuts at: 0 594 2883
Size: 594 2289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1578 2126 2742 2883
Size: 1578 548 616 141
Fragments arranged by size:
1578 616 548 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 789 997 1475 1504 1779 2271 2293
Size: 607 182 208 478 29 275 492 22
Cuts at: 2293 2731 2883
Size: 438 152
Fragments arranged by size:
607 492 478 438 275 208 182 152
29 22
Bpu10I CC'TnA_GC
Cuts at: 0 761 2828 2883
Size: 761 2067 55
Fragments arranged by size:
2067 761 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 2883
Size: 616 2267
BsaI GGTCTCn'nnnn_
Cuts at: 0 2362 2883
Size: 2362 521
BsaHI Gr'CG_yC
Cuts at: 0 22 2252 2775 2883
Size: 22 2230 523 108
Fragments arranged by size:
2230 523 108 22
BsaWI w'CCGG_w
Cuts at: 0 386 2204 2883
Size: 386 1818 679
Fragments arranged by size:
1818 679 386
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 362 392 417 447 2644 2674
Size: 17 30 315 30 25 30 2197 30
Cuts at: 2674 2883
Size: 209
Fragments arranged by size:
2197 315 209 30 30 30 30 25
17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1390 1472 2520 2665 2668 2731 2883
Size: 1390 82 1048 145 3 63 152
Fragments arranged by size:
1390 1048 152 145 82 63 3
BseYI C'CCAG_C
Cuts at: 0 277 357 1719 2267 2346 2883
Size: 277 80 1362 548 79 537
Fragments arranged by size:
1362 548 537 277 80 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1212 2185 2883
Size: 1212 973 698
Fragments arranged by size:
1212 973 698
BsiEI CG_ry'CG
Cuts at: 0 1467 2841 2883
Size: 1467 1374 42
Fragments arranged by size:
1467 1374 42
BsiHKAI G_wGCw'C
Cuts at: 0 454 632 2555 2883
Size: 454 178 1923 328
Fragments arranged by size:
1923 454 328 178
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1150 1788 2259 2883
Size: 1150 638 471 624
Fragments arranged by size:
1150 638 624 471
Bsp1286I G_dGCh'C
Cuts at: 0 227 375 454 522 632 1049 1946 2555
Size: 227 148 79 68 110 417 897 609
Cuts at: 2555 2564 2834 2883
Size: 9 270 49
Fragments arranged by size:
897 609 417 270 227 148 110 79
68 49 9
BsrBI CCG'CTC
Cuts at: 0 536 2768 2883
Size: 536 2232 115
Fragments arranged by size:
2232 536 115
BsrDI GCAATG_nn'
Cuts at: 0 1541 2277 2702 2883
Size: 1541 736 425 181
Fragments arranged by size:
1541 736 425 181
BsrFI r'CCGG_y
Cuts at: 0 827 2840 2883
Size: 827 2013 43
Fragments arranged by size:
2013 827 43
BsrGI T'GTAC_A
Cuts at: 0 509 1133 2883
Size: 509 624 1750
Fragments arranged by size:
1750 624 509
BssHII G'CGCG_C
Cuts at: 0 501 2883
Size: 501 2382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2500 2883
Size: 2500 383
BstEII G'GTnAC_C
Cuts at: 0 1951 2883
Size: 1951 932
BstXI CCAn_nnnn'nTGG
Cuts at: 0 756 2883
Size: 756 2127
BstYI r'GATC_y
Cuts at: 0 1035 1205 2883
Size: 1035 170 1678
Fragments arranged by size:
1678 1035 170
Bsu36I CC'TnA_GG
Cuts at: 0 1373 2237 2883
Size: 1373 864 646
Fragments arranged by size:
1373 864 646
BtgI C'CryG_G
Cuts at: 0 569 2883
Size: 569 2314
BtsI GCAGTG_nn'
Cuts at: 0 465 512 2453 2883
Size: 465 47 1941 430
Fragments arranged by size:
1941 465 430 47
EaeI y'GGCC_r
Cuts at: 0 245 544 1464 1566 2883
Size: 245 299 920 102 1317
Fragments arranged by size:
1317 920 299 245 102
EagI C'GGCC_G
Cuts at: 0 1464 2883
Size: 1464 1419
EarI CTCTTCn'nnn_
Cuts at: 0 34 1548 2883
Size: 34 1514 1335
Fragments arranged by size:
1514 1335 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1006 2883
Size: 25 981 1877
Fragments arranged by size:
1877 981 25
EcoICRI GAG'CTC
Cuts at: 0 630 2883
Size: 630 2253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 789 997 1081 1127 1475 1504 1609
Size: 607 182 208 84 46 348 29 105
Cuts at: 1609 1779 2271 2293 2731 2883
Size: 170 492 22 438 152
Fragments arranged by size:
607 492 438 348 208 182 170 152
105 84 46 29 22
EcoO109I rG'GnC_Cy
Cuts at: 0 132 223 351 1712 2808 2821 2883
Size: 132 91 128 1361 1096 13 62
Fragments arranged by size:
1361 1096 132 128 91 62 13
FspI TGC'GCA
Cuts at: 0 2572 2883
Size: 2572 311
HaeII r_GCGC'y
Cuts at: 0 25 586 1068 2778 2883
Size: 25 561 482 1710 105
Fragments arranged by size:
1710 561 482 105 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2385 2417 2644 2676 2883
Size: 2385 32 227 32 207
Fragments arranged by size:
2385 227 207 32 32
KasI G'GCGC_C
Cuts at: 0 21 2774 2883
Size: 21 2753 109
Fragments arranged by size:
2753 109 21
KpnI G_GTAC'C
Cuts at: 0 129 2883
Size: 129 2754
MfeI C'AATT_G
Cuts at: 0 1194 2883
Size: 1194 1689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 2582 2651 2883
Size: 1340 1242 69 232
Fragments arranged by size:
1340 1242 232 69
MscI TGG'CCA
Cuts at: 0 247 546 1568 2883
Size: 247 299 1022 1315
Fragments arranged by size:
1315 1022 299 247
MslI CAynn'nnrTG
Cuts at: 0 724 754 2883
Size: 724 30 2129
Fragments arranged by size:
2129 724 30
MspA1I CmG'CkG
Cuts at: 0 284 571 1042 1404 1589 1723 2271 2883
Size: 284 287 471 362 185 134 548 612
Fragments arranged by size:
612 548 471 362 287 284 185 134
NarI GG'CG_CC
Cuts at: 0 22 2775 2883
Size: 22 2753 108
Fragments arranged by size:
2753 108 22
NspI r_CATG'y
Cuts at: 0 1855 2883
Size: 1855 1028
PasI CC'CwG_GG
Cuts at: 0 107 1938 2816 2883
Size: 107 1831 878 67
Fragments arranged by size:
1831 878 107 67
PciI A'CATG_T
Cuts at: 0 1851 2883
Size: 1851 1032
PflMI CCAn_nnn'nTGG
Cuts at: 0 411 1734 2054 2883
Size: 411 1323 320 829
Fragments arranged by size:
1323 829 411 320
PfoI T'CCnGG_A
Cuts at: 0 256 421 1391 2287 2883
Size: 256 165 970 896 596
Fragments arranged by size:
970 896 596 256 165
PpuMI rG'GwC_Cy
Cuts at: 0 351 1712 2821 2883
Size: 351 1361 1109 62
Fragments arranged by size:
1361 1109 351 62
PshAI GACnn'nnGTC
Cuts at: 0 1735 2883
Size: 1735 1148
PsiI TTA'TAA
Cuts at: 0 2879 2883
Size: 2879 4
PspOMI G'GGCC_C
Cuts at: 0 223 2883
Size: 223 2660
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1153 1185 2883
Size: 1153 32 1698
Fragments arranged by size:
1698 1153 32
PstI C_TGCA'G
Cuts at: 0 739 2883
Size: 739 2144
PvuII CAG'CTG
Cuts at: 0 1042 1723 2271 2883
Size: 1042 681 548 612
Fragments arranged by size:
1042 681 612 548
SacI G_AGCT'C
Cuts at: 0 632 2883
Size: 632 2251
SacII CC_GC'GG
Cuts at: 0 572 2883
Size: 572 2311
SanDI GG'GwC_CC
Cuts at: 0 1712 2821 2883
Size: 1712 1109 62
Fragments arranged by size:
1712 1109 62
SexAI A'CCwGG_T
Cuts at: 0 146 2883
Size: 146 2737
SfcI C'TryA_G
Cuts at: 0 432 735 2883
Size: 432 303 2148
Fragments arranged by size:
2148 432 303
SfoI GGC'GCC
Cuts at: 0 23 2776 2883
Size: 23 2753 107
Fragments arranged by size:
2753 107 23
SmaI CCC'GGG
Cuts at: 0 44 376 2883
Size: 44 332 2507
Fragments arranged by size:
2507 332 44
SmlI C'TyrA_G
Cuts at: 0 631 2883
Size: 631 2252
StuI AGG'CCT
Cuts at: 0 559 2883
Size: 559 2324
StyI C'CwwG_G
Cuts at: 0 227 1427 1667 2225 2883
Size: 227 1200 240 558 658
Fragments arranged by size:
1200 658 558 240 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 875 1169 2795 2883
Size: 291 584 294 1626 88
Fragments arranged by size:
1626 584 294 291 88
TatI w'GTAC_w
Cuts at: 0 509 1133 2883
Size: 509 624 1750
Fragments arranged by size:
1750 624 509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 362 373 394 405 2883
Size: 17 32 313 11 21 11 2478
Fragments arranged by size:
2478 313 32 21 17 11 11
Tth111I GACn'n_nGTC
Cuts at: 0 2628 2883
Size: 2628 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1425 2883
Size: 1425 1458
XmaI C'CCGG_G
Cuts at: 0 42 374 2883
Size: 42 332 2509
Fragments arranged by size:
2509 332 42
XmnI GAAnn'nnTTC
Cuts at: 0 334 2883
Size: 334 2549
ZraI GAC'GTC
Cuts at: 0 2253 2883
Size: 2253 630
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I KasI KpnI MfeI MmeI MscI
MslI MspA1I NarI NspI PasI PciI PflMI PfoI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI
SacII SanDI SexAI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BfrBI BmrI BmtI BsaAI BsaBI BsiWI BsmI BspEI
BspHI BspMI BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PspXI PvuI RsrII SalI SapI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI