Restriction map for
pF1KB8554
(Linear) (Six-base) MAPSORT of: KIBB8554.seq Check: 7189 from: 1 to: 2724
KIBB8554.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2377 2724
Size: 2377 347
AccI GT'mk_AC
Cuts at: 0 2362 2724
Size: 2362 362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1832 2082 2462 2724
Size: 220 1612 250 380 262
Fragments arranged by size:
1612 380 262 250 220
AflIII A'CryG_T
Cuts at: 0 311 2724
Size: 311 2413
AleI CACnn'nnGTG
Cuts at: 0 1798 2724
Size: 1798 926
ApoI r'AATT_y
Cuts at: 0 781 1081 1095 1347 2724
Size: 781 300 14 252 1377
Fragments arranged by size:
1377 781 300 252 14
AseI AT'TA_AT
Cuts at: 0 367 2724
Size: 367 2357
AvaI C'yCGr_G
Cuts at: 0 578 714 824 1172 2724
Size: 578 136 110 348 1552
Fragments arranged by size:
1552 578 348 136 110
AvrII C'CTAG_G
Cuts at: 0 1962 2724
Size: 1962 762
BamHI G'GATC_C
Cuts at: 0 68 934 2148 2724
Size: 68 866 1214 576
Fragments arranged by size:
1214 866 576 68
BanI G'GyrC_C
Cuts at: 0 183 1755 2724
Size: 183 1572 969
Fragments arranged by size:
1572 969 183
BanII G_rGCy'C
Cuts at: 0 1515 2724
Size: 1515 1209
BbsI GAAGACnn'nnnn_
Cuts at: 0 1505 1950 2724
Size: 1505 445 774
Fragments arranged by size:
1505 774 445
BciVI GTATCCnnnnn_n'
Cuts at: 0 1270 2026 2254 2278 2724
Size: 1270 756 228 24 446
Fragments arranged by size:
1270 756 446 228 24
BclI T'GATC_A
Cuts at: 0 2090 2724
Size: 2090 634
BfrBI ATG'CAT
Cuts at: 0 529 2724
Size: 529 2195
BglII A'GATC_T
Cuts at: 0 710 1263 2724
Size: 710 553 1461
Fragments arranged by size:
1461 710 553
BmrI ACTGGGnnnn_n'
Cuts at: 0 646 2409 2724
Size: 646 1763 315
Fragments arranged by size:
1763 646 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 797 1180 2724
Size: 247 550 383 1544
Fragments arranged by size:
1544 550 383 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2203 2724
Size: 2203 521
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 1674 2724
Size: 154 1520 1050
Fragments arranged by size:
1520 1050 154
BsaAI yAC'GTr
Cuts at: 0 314 763 1869 2724
Size: 314 449 1106 855
Fragments arranged by size:
1106 855 449 314
BsaBI GATnn'nnATC
Cuts at: 0 2434 2724
Size: 2434 290
BsaHI Gr'CG_yC
Cuts at: 0 446 1537 2724
Size: 446 1091 1187
Fragments arranged by size:
1187 1091 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2117 2147 2724
Size: 2117 30 577
Fragments arranged by size:
2117 577 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 979 1105 2574 2724
Size: 979 126 1469 150
Fragments arranged by size:
1469 979 150 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1231 2724
Size: 379 852 1493
Fragments arranged by size:
1493 852 379
BsiHKAI G_wGCw'C
Cuts at: 0 319 1874 2219 2719 2724
Size: 319 1555 345 500 5
Fragments arranged by size:
1555 500 345 319 5
BsmI GAATG_Cn'
Cuts at: 0 1737 2724
Size: 1737 987
Bsp1286I G_dGCh'C
Cuts at: 0 319 1515 1874 2219 2719 2724
Size: 319 1196 359 345 500 5
Fragments arranged by size:
1196 500 359 345 319 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2377 2724
Size: 2377 347
BsrBI CCG'CTC
Cuts at: 0 689 2711 2724
Size: 689 2022 13
Fragments arranged by size:
2022 689 13
BsrDI GCAATG_nn'
Cuts at: 0 737 2724
Size: 737 1987
BsrGI T'GTAC_A
Cuts at: 0 2432 2724
Size: 2432 292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1254 1703 2724
Size: 1254 449 1021
Fragments arranged by size:
1254 1021 449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 2122 2724
Size: 123 1999 602
Fragments arranged by size:
1999 602 123
BstYI r'GATC_y
Cuts at: 0 68 408 710 934 1263 2148 2193 2724
Size: 68 340 302 224 329 885 45 531
Fragments arranged by size:
885 531 340 329 302 224 68 45
BstZ17I GTA'TAC
Cuts at: 0 2363 2724
Size: 2363 361
BtgI C'CryG_G
Cuts at: 0 614 1232 2724
Size: 614 618 1492
Fragments arranged by size:
1492 618 614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1770 2724
Size: 1770 954
BtsI GCAGTG_nn'
Cuts at: 0 836 1365 1513 2724
Size: 836 529 148 1211
Fragments arranged by size:
1211 836 529 148
ClaI AT'CG_AT
Cuts at: 0 1026 2724
Size: 1026 1698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1779 1814 2724
Size: 1779 35 910
Fragments arranged by size:
1779 910 35
EaeI y'GGCC_r
Cuts at: 0 1464 2724
Size: 1464 1260
EarI CTCTTCn'nnn_
Cuts at: 0 157 2724
Size: 157 2567
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 578 2724
Size: 578 2146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 247 797 1180 1832 2082 2462 2724
Size: 220 27 550 383 652 250 380 262
Fragments arranged by size:
652 550 383 380 262 250 220 27
EcoO109I rG'GnC_Cy
Cuts at: 0 264 1593 2724
Size: 264 1329 1131
Fragments arranged by size:
1329 1131 264
EcoRI G'AATT_C
Cuts at: 0 1081 2724
Size: 1081 1643
EcoRV GAT'ATC
Cuts at: 0 1416 2724
Size: 1416 1308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1342 2580 2724
Size: 1342 1238 144
Fragments arranged by size:
1342 1238 144
HaeII r_GCGC'y
Cuts at: 0 2059 2724
Size: 2059 665
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 559 591 1636 1668 2117 2149
Size: 301 32 226 32 1045 32 449 32
Cuts at: 2149 2178 2210 2252 2284 2724
Size: 29 32 42 32 440
Fragments arranged by size:
1045 449 440 301 226 42 32 32
32 32 32 32 29
MfeI C'AATT_G
Cuts at: 0 245 1010 2724
Size: 245 765 1714
Fragments arranged by size:
1714 765 245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1974 2724
Size: 305 1669 750
Fragments arranged by size:
1669 750 305
MslI CAynn'nnrTG
Cuts at: 0 79 121 639 1798 2724
Size: 79 42 518 1159 926
Fragments arranged by size:
1159 926 518 79 42
MspA1I CmG'CkG
Cuts at: 0 2085 2184 2584 2724
Size: 2085 99 400 140
Fragments arranged by size:
2085 400 140 99
NcoI C'CATG_G
Cuts at: 0 614 1232 2724
Size: 614 618 1492
Fragments arranged by size:
1492 618 614
NdeI CA'TA_TG
Cuts at: 0 1939 2724
Size: 1939 785
NsiI A_TGCA'T
Cuts at: 0 531 2724
Size: 531 2193
NspI r_CATG'y
Cuts at: 0 1506 1977 2496 2724
Size: 1506 471 519 228
Fragments arranged by size:
1506 519 471 228
PasI CC'CwG_GG
Cuts at: 0 1745 2724
Size: 1745 979
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 775 2724
Size: 615 160 1949
Fragments arranged by size:
1949 615 160
PfoI T'CCnGG_A
Cuts at: 0 1401 2724
Size: 1401 1323
PmlI CAC'GTG
Cuts at: 0 314 763 2724
Size: 314 449 1961
Fragments arranged by size:
1961 449 314
PpuMI rG'GwC_Cy
Cuts at: 0 264 1593 2724
Size: 264 1329 1131
Fragments arranged by size:
1329 1131 264
PshAI GACnn'nnGTC
Cuts at: 0 1813 2724
Size: 1813 911
PsiI TTA'TAA
Cuts at: 0 2521 2724
Size: 2521 203
PspXI vC'TCGA_Gb
Cuts at: 0 824 2724
Size: 824 1900
PstI C_TGCA'G
Cuts at: 0 462 1549 2724
Size: 462 1087 1175
Fragments arranged by size:
1175 1087 462
PvuII CAG'CTG
Cuts at: 0 2085 2584 2724
Size: 2085 499 140
Fragments arranged by size:
2085 499 140
SanDI GG'GwC_CC
Cuts at: 0 1593 2724
Size: 1593 1131
SexAI A'CCwGG_T
Cuts at: 0 559 1637 2724
Size: 559 1078 1087
Fragments arranged by size:
1087 1078 559
SfcI C'TryA_G
Cuts at: 0 458 1545 2687 2724
Size: 458 1087 1142 37
Fragments arranged by size:
1142 1087 458 37
SmlI C'TyrA_G
Cuts at: 0 578 714 824 2218 2724
Size: 578 136 110 1394 506
Fragments arranged by size:
1394 578 506 136 110
SphI G_CATG'C
Cuts at: 0 2496 2724
Size: 2496 228
SspI AAT'ATT
Cuts at: 0 1775 2027 2724
Size: 1775 252 697
Fragments arranged by size:
1775 697 252
StuI AGG'CCT
Cuts at: 0 700 2724
Size: 700 2024
StyI C'CwwG_G
Cuts at: 0 614 1232 1962 2121 2507 2724
Size: 614 618 730 159 386 217
Fragments arranged by size:
730 618 614 386 217 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 429 721 2724
Size: 283 146 292 2003
Fragments arranged by size:
2003 292 283 146
TatI w'GTAC_w
Cuts at: 0 1451 2287 2432 2724
Size: 1451 836 145 292
Fragments arranged by size:
1451 836 292 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 411 666 698 740 772 2724
Size: 379 32 255 32 42 32 1952
Fragments arranged by size:
1952 379 255 42 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 1725 2724
Size: 288 1437 999
Fragments arranged by size:
1437 999 288
XhoI C'TCGA_G
Cuts at: 0 578 714 824 2724
Size: 578 136 110 1900
Fragments arranged by size:
1900 578 136 110
Enzymes that do cut:
AarI AccI AcuI AflIII AleI ApoI AseI AvaI
AvrII BamHI BanI BanII BbsI BciVI BclI BfrBI
BglII BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrGI BstAPI BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI EaeI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I MfeI MmeI MslI
MspA1I NcoI NdeI NsiI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PstI PvuII SanDI
SexAI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI BaeI BbeI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrFI BssHII BssSI BstBI BstEII Bsu36I DraI DraIII
DrdI EagI EcoICRI EcoNI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI PciI PmeI PpiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI Tth111I XbaI XmaI XmnI ZraI