Restriction map for
pF1KB8562
(Linear) (Six-base) MAPSORT of: KIBB8562.seq Check: 2142 from: 1 to: 2733
KIBB8562.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
Acc65I G'GTAC_C
Cuts at: 0 157 1738 2733
Size: 157 1581 995
Fragments arranged by size:
1581 995 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2616 2632 2733
Size: 2616 16 101
Fragments arranged by size:
2616 101 16
AflIII A'CryG_T
Cuts at: 0 1667 2733
Size: 1667 1066
AhdI GACnn_n'nnGTC
Cuts at: 0 1445 2293 2733
Size: 1445 848 440
Fragments arranged by size:
1445 848 440
AleI CACnn'nnGTG
Cuts at: 0 682 1333 2182 2733
Size: 682 651 849 551
Fragments arranged by size:
849 682 651 551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 973 2733
Size: 973 1760
AlwNI CAG_nnn'CTG
Cuts at: 0 189 216 561 993 1215 1374 2245 2655
Size: 189 27 345 432 222 159 871 410
Cuts at: 2655 2733
Size: 78
Fragments arranged by size:
871 432 410 345 222 189 159 78
27
ApaI G_GGCC'C
Cuts at: 0 468 1102 1696 2733
Size: 468 634 594 1037
Fragments arranged by size:
1037 634 594 468
ApoI r'AATT_y
Cuts at: 0 644 2733
Size: 644 2089
AvaI C'yCGr_G
Cuts at: 0 79 153 1934 2733
Size: 79 74 1781 799
Fragments arranged by size:
1781 799 79 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 141 174 2434 2467 2733
Size: 141 33 2260 33 266
Fragments arranged by size:
2260 266 141 33 33
BamHI G'GATC_C
Cuts at: 0 546 2733
Size: 546 2187
BanI G'GyrC_C
Cuts at: 0 157 777 1005 1160 1677 1738 1899 2733
Size: 157 620 228 155 517 61 161 834
Fragments arranged by size:
834 620 517 228 161 157 155 61
BanII G_rGCy'C
Cuts at: 0 468 1102 1368 1696 2098 2333 2733
Size: 468 634 266 328 402 235 400
Fragments arranged by size:
634 468 402 400 328 266 235
BbeI G_GCGC'C
Cuts at: 0 1164 2733
Size: 1164 1569
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 71 330 431 1657 2013 2491 2733
Size: 32 39 259 101 1226 356 478 242
Fragments arranged by size:
1226 478 356 259 242 101 39 32
BbvCI CC'TCA_GC
Cuts at: 0 1929 2733
Size: 1929 804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1332 1366 2733
Size: 1332 34 1367
Fragments arranged by size:
1367 1332 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1869 2733
Size: 1869 864
BclI T'GATC_A
Cuts at: 0 1619 1844 2733
Size: 1619 225 889
Fragments arranged by size:
1619 889 225
BfrBI ATG'CAT
Cuts at: 0 1198 2733
Size: 1198 1535
BglI GCCn_nnn'nGGC
Cuts at: 0 898 2569 2607 2733
Size: 898 1671 38 126
Fragments arranged by size:
1671 898 126 38
BglII A'GATC_T
Cuts at: 0 1565 1625 2733
Size: 1565 60 1108
Fragments arranged by size:
1565 1108 60
BlpI GC'TnA_GC
Cuts at: 0 1367 2398 2733
Size: 1367 1031 335
Fragments arranged by size:
1367 1031 335
Bme1580I G_kGCm'C
Cuts at: 0 468 622 632 1102 1682 1696 2468 2733
Size: 468 154 10 470 580 14 772 265
Fragments arranged by size:
772 580 470 468 265 154 14 10
BmgBI CAC'GTC
Cuts at: 0 2393 2733
Size: 2393 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 1966 2733
Size: 1966 767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1587 1626 2733
Size: 1587 39 1107
Fragments arranged by size:
1587 1107 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 271 333 407 512 520 604 730
Size: 67 204 62 74 105 8 84 126
Cuts at: 730 778 1050 2059 2733
Size: 48 272 1009 674
Fragments arranged by size:
1009 674 272 204 126 105 84 74
67 62 48 8
Bpu10I CC'TnA_GC
Cuts at: 0 1929 2075 2733
Size: 1929 146 658
Fragments arranged by size:
1929 658 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 1432 2733
Size: 367 1065 1301
Fragments arranged by size:
1301 1065 367
BsaBI GATnn'nnATC
Cuts at: 0 1624 2733
Size: 1624 1109
BsaHI Gr'CG_yC
Cuts at: 0 1161 2733
Size: 1161 1572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2635 2665 2733
Size: 2635 30 68
Fragments arranged by size:
2635 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 52 109 268 466 567 1705 1917
Size: 19 33 57 159 198 101 1138 212
Cuts at: 1917 2704 2733
Size: 787 29
Fragments arranged by size:
1138 787 212 198 159 101 57 33
29 19
BseYI C'CCAG_C
Cuts at: 0 239 723 804 836 1359 1940 2733
Size: 239 484 81 32 523 581 793
Fragments arranged by size:
793 581 523 484 239 81 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1033 1229 1357 2733
Size: 907 126 196 128 1376
Fragments arranged by size:
1376 907 196 128 126
BsiHKAI G_wGCw'C
Cuts at: 0 1730 2733
Size: 1730 1003
BsmI GAATG_Cn'
Cuts at: 0 1198 1343 2733
Size: 1198 145 1390
Fragments arranged by size:
1390 1198 145
Bsp1286I G_dGCh'C
Cuts at: 0 468 622 632 1102 1368 1682 1696 1730
Size: 468 154 10 470 266 314 14 34
Cuts at: 1730 2098 2333 2468 2733
Size: 368 235 135 265
Fragments arranged by size:
470 468 368 314 266 265 235 154
135 34 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 2123 2733
Size: 374 1749 610
Fragments arranged by size:
1749 610 374
BsrBI CCG'CTC
Cuts at: 0 2625 2733
Size: 2625 108
BsrFI r'CCGG_y
Cuts at: 0 99 717 774 1735 2559 2733
Size: 99 618 57 961 824 174
Fragments arranged by size:
961 824 618 174 99 57
BssHII G'CGCG_C
Cuts at: 0 235 2733
Size: 235 2498
BstAPI GCAn_nnn'nTGC
Cuts at: 0 720 993 2733
Size: 720 273 1740
Fragments arranged by size:
1740 720 273
BstBI TT'CG_AA
Cuts at: 0 1029 2733
Size: 1029 1704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2325 2733
Size: 2325 408
BstYI r'GATC_y
Cuts at: 0 431 546 1565 1625 2255 2733
Size: 431 115 1019 60 630 478
Fragments arranged by size:
1019 630 478 431 115 60
Bsu36I CC'TnA_GG
Cuts at: 0 440 812 1688 2733
Size: 440 372 876 1045
Fragments arranged by size:
1045 876 440 372
BtsI GCAGTG_nn'
Cuts at: 0 991 1328 1340 2540 2733
Size: 991 337 12 1200 193
Fragments arranged by size:
1200 991 337 193 12
ClaI AT'CG_AT
Cuts at: 0 827 2733
Size: 827 1906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1943 1978 2043 2078 2733
Size: 1943 35 65 35 655
Fragments arranged by size:
1943 655 65 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1825 2733
Size: 1825 908
EaeI y'GGCC_r
Cuts at: 0 392 843 1733 2144 2720 2733
Size: 392 451 890 411 576 13
Fragments arranged by size:
890 576 451 411 392 13
EarI CTCTTCn'nnn_
Cuts at: 0 601 1138 1354 2733
Size: 601 537 216 1379
Fragments arranged by size:
1379 601 537 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1989 2733
Size: 1989 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 271 333 407 512 520 604 730
Size: 67 204 62 74 105 8 84 126
Cuts at: 730 778 1050 2059 2616 2632 2733
Size: 48 272 1009 557 16 101
Fragments arranged by size:
1009 557 272 204 126 105 101 84
74 67 62 48 16 8
EcoO109I rG'GnC_Cy
Cuts at: 0 275 465 504 808 816 1080 1098 1140
Size: 275 190 39 304 8 264 18 42
Cuts at: 1140 1212 1396 1692 1693 1827 2733
Size: 72 184 296 1 134 906
Fragments arranged by size:
906 304 296 275 264 190 184 134
72 42 39 18 8 1
EcoRI G'AATT_C
Cuts at: 0 644 2733
Size: 644 2089
EcoRV GAT'ATC
Cuts at: 0 1155 2733
Size: 1155 1578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2697 2733
Size: 2697 36
FspI TGC'GCA
Cuts at: 0 1765 2733
Size: 1765 968
HaeII r_GCGC'y
Cuts at: 0 1038 1164 2733
Size: 1038 126 1569
Fragments arranged by size:
1569 1038 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 423 455 964 996 1431 1463 2279 2311
Size: 423 32 509 32 435 32 816 32
Cuts at: 2311 2733
Size: 422
Fragments arranged by size:
816 509 435 423 422 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1160 2733
Size: 1160 1573
KpnI G_GTAC'C
Cuts at: 0 161 1742 2733
Size: 161 1581 991
Fragments arranged by size:
1581 991 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 1946 2733
Size: 1292 654 787
Fragments arranged by size:
1292 787 654
MscI TGG'CCA
Cuts at: 0 394 2722 2733
Size: 394 2328 11
Fragments arranged by size:
2328 394 11
MslI CAynn'nnrTG
Cuts at: 0 682 862 1333 1984 2182 2218 2323 2459
Size: 682 180 471 651 198 36 105 136
Cuts at: 2459 2728 2733
Size: 269 5
Fragments arranged by size:
682 651 471 269 198 180 136 105
36 5
MspA1I CmG'CkG
Cuts at: 0 751 2277 2402 2604 2733
Size: 751 1526 125 202 129
Fragments arranged by size:
1526 751 202 129 125
NaeI GCC'GGC
Cuts at: 0 101 2561 2733
Size: 101 2460 172
Fragments arranged by size:
2460 172 101
NarI GG'CG_CC
Cuts at: 0 1161 2733
Size: 1161 1572
NgoMIV G'CCGG_C
Cuts at: 0 99 2559 2733
Size: 99 2460 174
Fragments arranged by size:
2460 174 99
NsiI A_TGCA'T
Cuts at: 0 1200 2733
Size: 1200 1533
NspI r_CATG'y
Cuts at: 0 2499 2733
Size: 2499 234
PasI CC'CwG_GG
Cuts at: 0 507 2239 2733
Size: 507 1732 494
Fragments arranged by size:
1732 507 494
PflMI CCAn_nnn'nTGG
Cuts at: 0 1215 1956 2733
Size: 1215 741 777
Fragments arranged by size:
1215 777 741
PfoI T'CCnGG_A
Cuts at: 0 707 941 1631 2036 2258 2733
Size: 707 234 690 405 222 475
Fragments arranged by size:
707 690 475 405 234 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 2733
Size: 862 32 1839
Fragments arranged by size:
1839 862 32
PpuMI rG'GwC_Cy
Cuts at: 0 808 1212 1396 1827 2733
Size: 808 404 184 431 906
Fragments arranged by size:
906 808 431 404 184
PsiI TTA'TAA
Cuts at: 0 1658 2733
Size: 1658 1075
PspOMI G'GGCC_C
Cuts at: 0 464 1098 1692 2733
Size: 464 634 594 1041
Fragments arranged by size:
1041 634 594 464
PstI C_TGCA'G
Cuts at: 0 14 185 528 743 952 1118 1335 2250
Size: 14 171 343 215 209 166 217 915
Cuts at: 2250 2733
Size: 483
Fragments arranged by size:
915 483 343 217 215 209 171 166
14
SanDI GG'GwC_CC
Cuts at: 0 1827 2733
Size: 1827 906
SapI GCTCTTCn'nnn_
Cuts at: 0 601 1138 2733
Size: 601 537 1595
Fragments arranged by size:
1595 601 537
SfcI C'TryA_G
Cuts at: 0 10 181 524 739 948 1114 1331 1645
Size: 10 171 343 215 209 166 217 314
Cuts at: 1645 2246 2733
Size: 601 487
Fragments arranged by size:
601 487 343 314 217 215 209 171
166 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2569 2733
Size: 2569 164
SfoI GGC'GCC
Cuts at: 0 1162 2733
Size: 1162 1571
SmaI CCC'GGG
Cuts at: 0 155 1936 2733
Size: 155 1781 797
Fragments arranged by size:
1781 797 155
SphI G_CATG'C
Cuts at: 0 2499 2733
Size: 2499 234
StyI C'CwwG_G
Cuts at: 0 344 2672 2733
Size: 344 2328 61
Fragments arranged by size:
2328 344 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 461 850 1655 1900 1967 2125 2212
Size: 231 230 389 805 245 67 158 87
Cuts at: 2212 2460 2471 2532 2733
Size: 248 11 61 201
Fragments arranged by size:
805 389 248 245 231 230 201 158
87 67 61 11
TatI w'GTAC_w
Cuts at: 0 1454 2358 2733
Size: 1454 904 375
Fragments arranged by size:
1454 904 375
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2635 2667 2733
Size: 2635 32 66
Fragments arranged by size:
2635 66 32
XbaI T'CTAG_A
Cuts at: 0 1449 2733
Size: 1449 1284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1845 2733
Size: 1845 888
XmaI C'CCGG_G
Cuts at: 0 153 1934 2733
Size: 153 1781 799
Fragments arranged by size:
1781 799 153
XmnI GAAnn'nnTTC
Cuts at: 0 656 2733
Size: 656 2077
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI
Bsu36I BtsI ClaI CspCI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PsiI PspOMI PstI SanDI SapI SfcI SfiI SfoI
SmaI SphI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BmtI BpuEI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstEII BstZ17I BtgI BtgZI DraI DraIII EagI
EcoICRI EcoNI FseI FspAI HincII HindIII HpaI MfeI
MluI NcoI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PshAI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XhoI ZraI