Restriction map for
pF1KB8564
(Linear) (Six-base) MAPSORT of: KIBB8564.seq Check: 8216 from: 1 to: 2733
KIBB8564.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2428 2733
Size: 2428 305
AatII G_ACGT'C
Cuts at: 0 359 2667 2733
Size: 359 2308 66
Fragments arranged by size:
2308 359 66
AccI GT'mk_AC
Cuts at: 0 1049 2733
Size: 1049 1684
Acc65I G'GTAC_C
Cuts at: 0 1317 1807 2213 2733
Size: 1317 490 406 520
Fragments arranged by size:
1317 520 490 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1853 2733
Size: 776 1077 880
Fragments arranged by size:
1077 880 776
AfeI AGC'GCT
Cuts at: 0 961 2733
Size: 961 1772
AgeI A'CCGG_T
Cuts at: 0 311 2210 2525 2733
Size: 311 1899 315 208
Fragments arranged by size:
1899 315 311 208
AleI CACnn'nnGTG
Cuts at: 0 1970 2733
Size: 1970 763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 593 626 1568 2733
Size: 593 33 942 1165
Fragments arranged by size:
1165 942 593 33
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1639 1671 2733
Size: 1639 32 1062
Fragments arranged by size:
1639 1062 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1120 1961 2733
Size: 1120 841 772
Fragments arranged by size:
1120 841 772
ApaI G_GGCC'C
Cuts at: 0 525 1471 1800 2733
Size: 525 946 329 933
Fragments arranged by size:
946 933 525 329
ApoI r'AATT_y
Cuts at: 0 1297 2733
Size: 1297 1436
AvaI C'yCGr_G
Cuts at: 0 248 694 1464 1733 2185 2630 2733
Size: 248 446 770 269 452 445 103
Fragments arranged by size:
770 452 446 445 269 248 103
AvrII C'CTAG_G
Cuts at: 0 1282 2733
Size: 1282 1451
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 381 414 2733
Size: 381 33 2319
Fragments arranged by size:
2319 381 33
BanI G'GyrC_C
Cuts at: 0 151 1317 1524 1768 1807 2089 2213 2528
Size: 151 1166 207 244 39 282 124 315
Cuts at: 2528 2733
Size: 205
Fragments arranged by size:
1166 315 282 244 207 205 151 124
39
BanII G_rGCy'C
Cuts at: 0 220 493 525 552 1457 1471 1800 2637
Size: 220 273 32 27 905 14 329 837
Cuts at: 2637 2733
Size: 96
Fragments arranged by size:
905 837 329 273 220 96 32 27
14
BbeI G_GCGC'C
Cuts at: 0 155 1528 2093 2733
Size: 155 1373 565 640
Fragments arranged by size:
1373 640 565 155
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 1271 2628 2733
Size: 764 507 1357 105
Fragments arranged by size:
1357 764 507 105
BciVI GTATCCnnnnn_n'
Cuts at: 0 653 2733
Size: 653 2080
BglI GCCn_nnn'nGGC
Cuts at: 0 529 1795 2733
Size: 529 1266 938
Fragments arranged by size:
1266 938 529
BglII A'GATC_T
Cuts at: 0 2603 2733
Size: 2603 130
BlpI GC'TnA_GC
Cuts at: 0 370 1456 2733
Size: 370 1086 1277
Fragments arranged by size:
1277 1086 370
Bme1580I G_kGCm'C
Cuts at: 0 525 540 1471 1773 1800 2396 2618 2733
Size: 525 15 931 302 27 596 222 115
Fragments arranged by size:
931 596 525 302 222 115 27 15
BmgBI CAC'GTC
Cuts at: 0 174 2391 2733
Size: 174 2217 342
Fragments arranged by size:
2217 342 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 2136 2689 2733
Size: 2136 553 44
Fragments arranged by size:
2136 553 44
BmtI G_CTAG'C
Cuts at: 0 919 2733
Size: 919 1814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1845 2733
Size: 1845 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1180 1244 1840 2360 2733
Size: 776 404 64 596 520 373
Fragments arranged by size:
776 596 520 404 373 64
Bpu10I CC'TnA_GC
Cuts at: 0 1715 2733
Size: 1715 1018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2456 2733
Size: 2456 277
BsaI GGTCTCn'nnnn_
Cuts at: 0 1147 2131 2733
Size: 1147 984 602
Fragments arranged by size:
1147 984 602
BsaHI Gr'CG_yC
Cuts at: 0 152 356 1525 2078 2090 2282 2664 2733
Size: 152 204 1169 553 12 192 382 69
Fragments arranged by size:
1169 553 382 204 192 152 69 12
BsaWI w'CCGG_w
Cuts at: 0 311 701 2210 2454 2525 2733
Size: 311 390 1509 244 71 208
Fragments arranged by size:
1509 390 311 244 208 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 275 305 1090 1120 1910 1940 2212 2242
Size: 275 30 785 30 790 30 272 30
Cuts at: 2242 2733
Size: 491
Fragments arranged by size:
790 785 491 275 272 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1256 1411 1426 1429 1751 1762 2002 2005
Size: 1256 155 15 3 322 11 240 3
Cuts at: 2005 2143 2662 2733
Size: 138 519 71
Fragments arranged by size:
1256 519 322 240 155 138 71 15
11 3 3
BseYI C'CCAG_C
Cuts at: 0 5 213 339 952 1070 1101 1113 1269
Size: 5 208 126 613 118 31 12 156
Cuts at: 1269 1447 1857 1880 1954 2072 2123 2546 2733
Size: 178 410 23 74 118 51 423 187
Fragments arranged by size:
613 423 410 208 187 178 156 126
118 118 74 51 31 23 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1894 2623 2733
Size: 863 1031 729 110
Fragments arranged by size:
1031 863 729 110
BsiEI CG_ry'CG
Cuts at: 0 2484 2586 2733
Size: 2484 102 147
Fragments arranged by size:
2484 147 102
BsiHKAI G_wGCw'C
Cuts at: 0 146 1457 2637 2733
Size: 146 1311 1180 96
Fragments arranged by size:
1311 1180 146 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 2733
Size: 363 2370
Bsp1286I G_dGCh'C
Cuts at: 0 146 220 493 525 540 552 1457 1471
Size: 146 74 273 32 15 12 905 14
Cuts at: 1471 1773 1800 2396 2618 2637 2733
Size: 302 27 596 222 19 96
Fragments arranged by size:
905 596 302 273 222 146 96 74
32 27 19 15 14 12
BspEI T'CCGG_A
Cuts at: 0 701 2733
Size: 701 2032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 2428 2634 2733
Size: 191 2237 206 99
Fragments arranged by size:
2237 206 191 99
BsrBI CCG'CTC
Cuts at: 0 837 2733
Size: 837 1896
BsrDI GCAATG_nn'
Cuts at: 0 610 2733
Size: 610 2123
BsrFI r'CCGG_y
Cuts at: 0 311 2210 2525 2733
Size: 311 1899 315 208
Fragments arranged by size:
1899 315 311 208
BssHII G'CGCG_C
Cuts at: 0 2159 2733
Size: 2159 574
BssSI C'ACGA_G
Cuts at: 0 659 2733
Size: 659 2074
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 331 850 2733
Size: 141 190 519 1883
Fragments arranged by size:
1883 519 190 141
BstEII G'GTnAC_C
Cuts at: 0 784 2733
Size: 784 1949
BstXI CCAn_nnnn'nTGG
Cuts at: 0 964 1818 1972 2733
Size: 964 854 154 761
Fragments arranged by size:
964 854 761 154
BstYI r'GATC_y
Cuts at: 0 2603 2733
Size: 2603 130
Bsu36I CC'TnA_GG
Cuts at: 0 1088 1178 1472 1802 2733
Size: 1088 90 294 330 931
Fragments arranged by size:
1088 931 330 294 90
BtgI C'CryG_G
Cuts at: 0 223 857 1203 1313 1325 1605 1925 2488
Size: 223 634 346 110 12 280 320 563
Cuts at: 2488 2733
Size: 245
Fragments arranged by size:
634 563 346 320 280 245 223 110
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 99 540 2057 2733
Size: 99 441 1517 676
Fragments arranged by size:
1517 676 441 99
BtsI GCAGTG_nn'
Cuts at: 0 130 325 897 933 2733
Size: 130 195 572 36 1800
Fragments arranged by size:
1800 572 195 130 36
ClaI AT'CG_AT
Cuts at: 0 428 2733
Size: 428 2305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1659 1673 1694 1708 2733
Size: 1659 14 21 14 1025
Fragments arranged by size:
1659 1025 21 14 14
DrdI GACnn_nn'nnGTC
Cuts at: 0 243 2733
Size: 243 2490
EaeI y'GGCC_r
Cuts at: 0 187 1922 2168 2481 2733
Size: 187 1735 246 313 252
Fragments arranged by size:
1735 313 252 246 187
EagI C'GGCC_G
Cuts at: 0 2481 2733
Size: 2481 252
EarI CTCTTCn'nnn_
Cuts at: 0 1000 1006 1334 1361 1382 1403 2733
Size: 1000 6 328 27 21 21 1330
Fragments arranged by size:
1330 1000 328 27 21 21 6
EcoICRI GAG'CTC
Cuts at: 0 1455 2635 2733
Size: 1455 1180 98
Fragments arranged by size:
1455 1180 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1180 1244 1840 1853 2360 2733
Size: 776 404 64 596 13 507 373
Fragments arranged by size:
776 596 507 404 373 64 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 2733
Size: 43 2690
EcoO109I rG'GnC_Cy
Cuts at: 0 521 1278 1468 1728 1797 1824 1865 2034
Size: 521 757 190 260 69 27 41 169
Cuts at: 2034 2595 2652 2728 2733
Size: 561 57 76 5
Fragments arranged by size:
757 561 521 260 190 169 76 69
57 41 27 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1011 2733
Size: 1011 1722
FspI TGC'GCA
Cuts at: 0 112 2733
Size: 112 2621
FspAI rTGC'GCAy
Cuts at: 0 112 2733
Size: 112 2621
HaeII r_GCGC'y
Cuts at: 0 155 370 963 1528 1907 2093 2698 2733
Size: 155 215 593 565 379 186 605 35
Fragments arranged by size:
605 593 565 379 215 186 155 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 275 307 539 571 1183 1215 1561 1593
Size: 275 32 232 32 612 32 346 32
Cuts at: 1593 2733
Size: 1140
Fragments arranged by size:
1140 612 346 275 232 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1583 2733
Size: 1583 1150
KasI G'GCGC_C
Cuts at: 0 151 1524 2089 2733
Size: 151 1373 565 644
Fragments arranged by size:
1373 644 565 151
KpnI G_GTAC'C
Cuts at: 0 1321 1811 2217 2733
Size: 1321 490 406 516
Fragments arranged by size:
1321 516 490 406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1121 1133 2255 2733
Size: 605 516 12 1122 478
Fragments arranged by size:
1122 605 516 478 12
MscI TGG'CCA
Cuts at: 0 2170 2733
Size: 2170 563
MslI CAynn'nnrTG
Cuts at: 0 1970 2733
Size: 1970 763
MspA1I CmG'CkG
Cuts at: 0 9 225 1173 2006 2562 2733
Size: 9 216 948 833 556 171
Fragments arranged by size:
948 833 556 216 171 9
NarI GG'CG_CC
Cuts at: 0 152 1525 2090 2733
Size: 152 1373 565 643
Fragments arranged by size:
1373 643 565 152
NcoI C'CATG_G
Cuts at: 0 1313 1605 2733
Size: 1313 292 1128
Fragments arranged by size:
1313 1128 292
NheI G'CTAG_C
Cuts at: 0 915 2733
Size: 915 1818
NspI r_CATG'y
Cuts at: 0 1982 2733
Size: 1982 751
PasI CC'CwG_GG
Cuts at: 0 907 1637 2311 2733
Size: 907 730 674 422
Fragments arranged by size:
907 730 674 422
PpuMI rG'GwC_Cy
Cuts at: 0 1728 1865 2034 2652 2728 2733
Size: 1728 137 169 618 76 5
Fragments arranged by size:
1728 618 169 137 76 5
PspOMI G'GGCC_C
Cuts at: 0 521 1467 1796 2733
Size: 521 946 329 937
Fragments arranged by size:
946 937 521 329
PspXI vC'TCGA_Gb
Cuts at: 0 1733 2733
Size: 1733 1000
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 292 324 2733
Size: 292 32 2409
Fragments arranged by size:
2409 292 32
PstI C_TGCA'G
Cuts at: 0 57 137 904 1172 1547 2733
Size: 57 80 767 268 375 1186
Fragments arranged by size:
1186 767 375 268 80 57
PvuII CAG'CTG
Cuts at: 0 9 1173 2733
Size: 9 1164 1560
Fragments arranged by size:
1560 1164 9
SacI G_AGCT'C
Cuts at: 0 1457 2637 2733
Size: 1457 1180 96
Fragments arranged by size:
1457 1180 96
SacII CC_GC'GG
Cuts at: 0 226 2733
Size: 226 2507
SanDI GG'GwC_CC
Cuts at: 0 1728 2733
Size: 1728 1005
SfcI C'TryA_G
Cuts at: 0 53 133 900 1168 1543 2733
Size: 53 80 767 268 375 1190
Fragments arranged by size:
1190 767 375 268 80 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 529 2733
Size: 529 2204
SfoI GGC'GCC
Cuts at: 0 153 1526 2091 2733
Size: 153 1373 565 642
Fragments arranged by size:
1373 642 565 153
SmaI CCC'GGG
Cuts at: 0 250 696 1466 2733
Size: 250 446 770 1267
Fragments arranged by size:
1267 770 446 250
SmlI C'TyrA_G
Cuts at: 0 1733 2471 2733
Size: 1733 738 262
Fragments arranged by size:
1733 738 262
SrfI GCCC'GGGC
Cuts at: 0 1466 2733
Size: 1466 1267
StuI AGG'CCT
Cuts at: 0 307 532 667 1093 1477 1593 2733
Size: 307 225 135 426 384 116 1140
Fragments arranged by size:
1140 426 384 307 225 135 116
StyI C'CwwG_G
Cuts at: 0 588 1282 1313 1597 1605 2733
Size: 588 694 31 284 8 1128
Fragments arranged by size:
1128 694 588 284 31 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 772 966 2657 2733
Size: 772 194 1691 76
Fragments arranged by size:
1691 772 194 76
TatI w'GTAC_w
Cuts at: 0 743 2733
Size: 743 1990
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1910 1942 2212 2244 2733
Size: 696 32 1182 32 270 32 489
Fragments arranged by size:
1182 696 489 270 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1948 2733
Size: 1948 785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 964 1227 2733
Size: 964 263 1506
Fragments arranged by size:
1506 964 263
XhoI C'TCGA_G
Cuts at: 0 1733 2733
Size: 1733 1000
XmaI C'CCGG_G
Cuts at: 0 248 694 1464 2733
Size: 248 446 770 1269
Fragments arranged by size:
1269 770 446 248
ZraI GAC'GTC
Cuts at: 0 357 2665 2733
Size: 357 2308 68
Fragments arranged by size:
2308 357 68
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AleI
AlfI AloI AlwNI ApaI ApoI AvaI AvrII BaeI
BanI BanII BbeI BbsI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PasI PpuMI PspOMI PspXI
PsrI PstI PvuII SacI SacII SanDI SfcI SfiI
SfoI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AflII AflIII AhdI ApaLI AscI AseI AsiSI
BamHI BbvCI BcgI BclI BfrBI BsaAI BsaBI BsiWI
BsmI BspHI BsrGI BstBI BstZ17I DraI DraIII EciI
EcoRI EcoRV FseI HincII HpaI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PvuI RsrII
SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI XmnI