Restriction map for
pF1KB8567
(Linear) (Six-base) MAPSORT of: KIBB8567.seq Check: 9081 from: 1 to: 1194
KIBB8567.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1194
Size: 1141 53
AflII C'TTAA_G
Cuts at: 0 807 1194
Size: 807 387
AflIII A'CryG_T
Cuts at: 0 563 1194
Size: 563 631
AlwNI CAG_nnn'CTG
Cuts at: 0 931 1194
Size: 931 263
ApoI r'AATT_y
Cuts at: 0 772 1078 1194
Size: 772 306 116
Fragments arranged by size:
772 306 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 1194
Size: 698 496
BbvCI CC'TCA_GC
Cuts at: 0 383 1139 1194
Size: 383 756 55
Fragments arranged by size:
756 383 55
BciVI GTATCCnnnnn_n'
Cuts at: 0 994 1194
Size: 994 200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 309 1194
Size: 309 885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1194
Size: 454 740
Bpu10I CC'TnA_GC
Cuts at: 0 383 1139 1194
Size: 383 756 55
Fragments arranged by size:
756 383 55
BsaBI GATnn'nnATC
Cuts at: 0 413 1194
Size: 413 781
BsaHI Gr'CG_yC
Cuts at: 0 15 1194
Size: 15 1179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 1156 1186 1194
Size: 544 30 582 30 8
Fragments arranged by size:
582 544 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 1194
Size: 682 512
BsrGI T'GTAC_A
Cuts at: 0 560 1194
Size: 560 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 931 1194
Size: 931 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 507 1050 1194
Size: 36 471 543 144
Fragments arranged by size:
543 471 144 36
Bsu36I CC'TnA_GG
Cuts at: 0 284 1194
Size: 284 910
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1068 1194
Size: 1068 126
BtsI GCAGTG_nn'
Cuts at: 0 399 1194
Size: 399 795
ClaI AT'CG_AT
Cuts at: 0 585 1194
Size: 585 609
EaeI y'GGCC_r
Cuts at: 0 1040 1194
Size: 1040 154
EarI CTCTTCn'nnn_
Cuts at: 0 299 468 1194
Size: 299 169 726
Fragments arranged by size:
726 299 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1141 1194
Size: 454 687 53
Fragments arranged by size:
687 454 53
EcoO109I rG'GnC_Cy
Cuts at: 0 268 1194
Size: 268 926
EcoRV GAT'ATC
Cuts at: 0 411 1194
Size: 411 783
HaeII r_GCGC'y
Cuts at: 0 7 1194
Size: 7 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 663 695 1156 1188 1194
Size: 627 32 4 32 461 32 6
Fragments arranged by size:
627 461 32 32 32 6 4
HincII GTy'rAC
Cuts at: 0 204 1194
Size: 204 990
HindIII A'AGCT_T
Cuts at: 0 320 619 1194
Size: 320 299 575
Fragments arranged by size:
575 320 299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1194
Size: 36 1158
MscI TGG'CCA
Cuts at: 0 1042 1194
Size: 1042 152
MslI CAynn'nnrTG
Cuts at: 0 505 1194
Size: 505 689
MspA1I CmG'CkG
Cuts at: 0 432 931 1143 1194
Size: 432 499 212 51
Fragments arranged by size:
499 432 212 51
NspI r_CATG'y
Cuts at: 0 495 567 1194
Size: 495 72 627
Fragments arranged by size:
627 495 72
PciI A'CATG_T
Cuts at: 0 563 1194
Size: 563 631
PfoI T'CCnGG_A
Cuts at: 0 610 1194
Size: 610 584
PpuMI rG'GwC_Cy
Cuts at: 0 268 1194
Size: 268 926
PstI C_TGCA'G
Cuts at: 0 394 551 1194
Size: 394 157 643
Fragments arranged by size:
643 394 157
PvuII CAG'CTG
Cuts at: 0 432 931 1143 1194
Size: 432 499 212 51
Fragments arranged by size:
499 432 212 51
SfcI C'TryA_G
Cuts at: 0 390 547 1194
Size: 390 157 647
Fragments arranged by size:
647 390 157
SmlI C'TyrA_G
Cuts at: 0 807 1194
Size: 807 387
SphI G_CATG'C
Cuts at: 0 495 1194
Size: 495 699
SspI AAT'ATT
Cuts at: 0 45 687 1194
Size: 45 642 507
Fragments arranged by size:
642 507 45
TatI w'GTAC_w
Cuts at: 0 240 560 1194
Size: 240 320 634
Fragments arranged by size:
634 320 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 1194
Size: 156 32 1006
Fragments arranged by size:
1006 156 32
Tth111I GACn'n_nGTC
Cuts at: 0 24 1194
Size: 24 1170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 867 1194
Size: 867 327
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI BbsI BbvCI BciVI
BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BsrGI
BstAPI BstXI Bsu36I BtgZI BtsI ClaI EaeI EarI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NspI PciI PfoI PpuMI PstI
PvuII SfcI SmlI SphI SspI TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI
BstZ17I BtgI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI StyI SwaI TaqII XbaI XhoI
XmaI XmnI ZraI