Restriction map for
pF1KB8569
(Linear) (Six-base) MAPSORT of: KIBB8569.seq Check: 6680 from: 1 to: 2313
KIBB8569.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 393 853 1375 1726 1798 2313
Size: 92 301 460 522 351 72 515
Fragments arranged by size:
522 515 460 351 301 92 72
AfeI AGC'GCT
Cuts at: 0 985 2313
Size: 985 1328
AflII C'TTAA_G
Cuts at: 0 1783 2313
Size: 1783 530
AgeI A'CCGG_T
Cuts at: 0 2081 2313
Size: 2081 232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 1319 2027 2313
Size: 679 640 708 286
Fragments arranged by size:
708 679 640 286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 2313
Size: 1435 32 846
Fragments arranged by size:
1435 846 32
AlwNI CAG_nnn'CTG
Cuts at: 0 31 70 128 293 352 586 859 1903
Size: 31 39 58 165 59 234 273 1044
Cuts at: 1903 2313
Size: 410
Fragments arranged by size:
1044 410 273 234 165 59 58 39
31
ApaI G_GGCC'C
Cuts at: 0 564 763 2313
Size: 564 199 1550
Fragments arranged by size:
1550 564 199
ApoI r'AATT_y
Cuts at: 0 1621 2313
Size: 1621 692
AvaI C'yCGr_G
Cuts at: 0 382 970 1068 2313
Size: 382 588 98 1245
Fragments arranged by size:
1245 588 382 98
BamHI G'GATC_C
Cuts at: 0 1214 1847 2313
Size: 1214 633 466
Fragments arranged by size:
1214 633 466
BanI G'GyrC_C
Cuts at: 0 154 454 917 1818 2313
Size: 154 300 463 901 495
Fragments arranged by size:
901 495 463 300 154
BanII G_rGCy'C
Cuts at: 0 564 763 866 2167 2313
Size: 564 199 103 1301 146
Fragments arranged by size:
1301 564 199 146 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 1363 1570 2239 2313
Size: 1363 207 669 74
Fragments arranged by size:
1363 669 207 74
BbvCI CC'TCA_GC
Cuts at: 0 211 353 362 1417 2254 2313
Size: 211 142 9 1055 837 59
Fragments arranged by size:
1055 837 211 142 59 9
BglI GCCn_nnn'nGGC
Cuts at: 0 758 1744 2197 2313
Size: 758 986 453 116
Fragments arranged by size:
986 758 453 116
BlpI GC'TnA_GC
Cuts at: 0 490 865 2313
Size: 490 375 1448
Fragments arranged by size:
1448 490 375
Bme1580I G_kGCm'C
Cuts at: 0 143 157 457 564 763 1236 2313
Size: 143 14 300 107 199 473 1077
Fragments arranged by size:
1077 473 300 199 143 107 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 2035 2309 2313
Size: 453 1582 274 4
Fragments arranged by size:
1582 453 274 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 454 663 1818 2197 2313
Size: 315 139 209 1155 379 116
Fragments arranged by size:
1155 379 315 209 139 116
Bpu10I CC'TnA_GC
Cuts at: 0 211 353 362 575 703 1417 2254 2313
Size: 211 142 9 213 128 714 837 59
Fragments arranged by size:
837 714 213 211 142 128 59 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1988 2119 2313
Size: 1988 131 194
Fragments arranged by size:
1988 194 131
BsaHI Gr'CG_yC
Cuts at: 0 1550 1594 2313
Size: 1550 44 719
Fragments arranged by size:
1550 719 44
BsaWI w'CCGG_w
Cuts at: 0 89 2081 2228 2313
Size: 89 1992 147 85
Fragments arranged by size:
1992 147 89 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2068 2098 2313
Size: 2068 30 215
Fragments arranged by size:
2068 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 91 802 1984 2089 2313
Size: 19 72 711 1182 105 224
Fragments arranged by size:
1182 711 224 105 72 19
BseYI C'CCAG_C
Cuts at: 0 159 366 586 651 826 1005 1896 2313
Size: 159 207 220 65 175 179 891 417
Fragments arranged by size:
891 417 220 207 179 175 159 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1243 1932 2313
Size: 917 326 689 381
Fragments arranged by size:
917 689 381 326
BsiHKAI G_wGCw'C
Cuts at: 0 866 1092 1637 2096 2313
Size: 866 226 545 459 217
Fragments arranged by size:
866 545 459 226 217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 964 2313
Size: 964 1349
Bsp1286I G_dGCh'C
Cuts at: 0 143 157 457 564 763 866 1092 1236
Size: 143 14 300 107 199 103 226 144
Cuts at: 1236 1637 2096 2167 2313
Size: 401 459 71 146
Fragments arranged by size:
459 401 300 226 199 146 144 143
107 103 71 14
BspEI T'CCGG_A
Cuts at: 0 89 2228 2313
Size: 89 2139 85
Fragments arranged by size:
2139 89 85
BspHI T'CATG_A
Cuts at: 0 1986 2313
Size: 1986 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1265 1358 1664 2313
Size: 1265 93 306 649
Fragments arranged by size:
1265 649 306 93
BsrBI CCG'CTC
Cuts at: 0 689 1146 2181 2313
Size: 689 457 1035 132
Fragments arranged by size:
1035 689 457 132
BsrDI GCAATG_nn'
Cuts at: 0 1301 2313
Size: 1301 1012
BsrFI r'CCGG_y
Cuts at: 0 1169 2081 2313
Size: 1169 912 232
Fragments arranged by size:
1169 912 232
BssSI C'ACGA_G
Cuts at: 0 142 2313
Size: 142 2171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 128 2313
Size: 128 2185
BstEII G'GTnAC_C
Cuts at: 0 1699 2313
Size: 1699 614
BstXI CCAn_nnnn'nTGG
Cuts at: 0 748 1273 2272 2313
Size: 748 525 999 41
Fragments arranged by size:
999 748 525 41
BstYI r'GATC_y
Cuts at: 0 1214 1847 2313
Size: 1214 633 466
Fragments arranged by size:
1214 633 466
Bsu36I CC'TnA_GG
Cuts at: 0 32 755 1852 2313
Size: 32 723 1097 461
Fragments arranged by size:
1097 723 461 32
BtgI C'CryG_G
Cuts at: 0 204 1599 2313
Size: 204 1395 714
Fragments arranged by size:
1395 714 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 56 2313
Size: 56 2257
BtsI GCAGTG_nn'
Cuts at: 0 32 1637 2313
Size: 32 1605 676
Fragments arranged by size:
1605 676 32
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 2313
Size: 517 1796
EaeI y'GGCC_r
Cuts at: 0 101 722 908 1037 1263 1329 1736 2021
Size: 101 621 186 129 226 66 407 285
Cuts at: 2021 2198 2313
Size: 177 115
Fragments arranged by size:
621 407 285 226 186 177 129 115
101 66
EarI CTCTTCn'nnn_
Cuts at: 0 68 1816 2313
Size: 68 1748 497
Fragments arranged by size:
1748 497 68
EcoICRI GAG'CTC
Cuts at: 0 864 2313
Size: 864 1449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 315 393 454 663 853 1375 1726
Size: 92 223 78 61 209 190 522 351
Cuts at: 1726 1798 1818 2197 2313
Size: 72 20 379 116
Fragments arranged by size:
522 379 351 223 209 190 116 92
78 72 61 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 35 578 1660 1813 1963 2257 2313
Size: 35 543 1082 153 150 294 56
Fragments arranged by size:
1082 543 294 153 150 56 35
EcoO109I rG'GnC_Cy
Cuts at: 0 111 186 290 357 531 759 1149 1745
Size: 111 75 104 67 174 228 390 596
Cuts at: 1745 2313
Size: 568
Fragments arranged by size:
596 568 390 228 174 111 104 75
67
HaeII r_GCGC'y
Cuts at: 0 416 672 987 1525 2313
Size: 416 256 315 538 788
Fragments arranged by size:
788 538 416 315 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 829 841 861 873 1275 1307 2313
Size: 829 12 20 12 402 32 1006
Fragments arranged by size:
1006 829 402 32 20 12 12
HindIII A'AGCT_T
Cuts at: 0 1780 2313
Size: 1780 533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 863 1793 2313
Size: 353 510 930 520
Fragments arranged by size:
930 520 510 353
MscI TGG'CCA
Cuts at: 0 103 724 910 1331 1738 2200 2313
Size: 103 621 186 421 407 462 113
Fragments arranged by size:
621 462 421 407 186 113 103
MslI CAynn'nnrTG
Cuts at: 0 724 746 1720 2313
Size: 724 22 974 593
Fragments arranged by size:
974 724 593 22
MspA1I CmG'CkG
Cuts at: 0 163 280 366 515 558 634 1005 1119
Size: 163 117 86 149 43 76 371 114
Cuts at: 1119 1320 1903 2007 2313
Size: 201 583 104 306
Fragments arranged by size:
583 371 306 201 163 149 117 114
104 86 76 43
NcoI C'CATG_G
Cuts at: 0 204 2313
Size: 204 2109
PasI CC'CwG_GG
Cuts at: 0 136 246 763 2313
Size: 136 110 517 1550
Fragments arranged by size:
1550 517 136 110
PflMI CCAn_nnn'nTGG
Cuts at: 0 293 586 2142 2313
Size: 293 293 1556 171
Fragments arranged by size:
1556 293 293 171
PfoI T'CCnGG_A
Cuts at: 0 331 1484 1505 1843 2313
Size: 331 1153 21 338 470
Fragments arranged by size:
1153 470 338 331 21
PpuMI rG'GwC_Cy
Cuts at: 0 186 357 1149 2313
Size: 186 171 792 1164
Fragments arranged by size:
1164 792 186 171
PshAI GACnn'nnGTC
Cuts at: 0 59 2313
Size: 59 2254
PspOMI G'GGCC_C
Cuts at: 0 560 759 2313
Size: 560 199 1554
Fragments arranged by size:
1554 560 199
PspXI vC'TCGA_Gb
Cuts at: 0 970 2313
Size: 970 1343
PstI C_TGCA'G
Cuts at: 0 27 133 1661 2313
Size: 27 106 1528 652
Fragments arranged by size:
1528 652 106 27
PvuII CAG'CTG
Cuts at: 0 366 515 634 1005 1320 2007 2313
Size: 366 149 119 371 315 687 306
Fragments arranged by size:
687 371 366 315 306 149 119
SacI G_AGCT'C
Cuts at: 0 866 2313
Size: 866 1447
SexAI A'CCwGG_T
Cuts at: 0 1862 2313
Size: 1862 451
SfcI C'TryA_G
Cuts at: 0 23 129 1657 1755 2313
Size: 23 106 1528 98 558
Fragments arranged by size:
1528 558 106 98 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 758 1744 2197 2313
Size: 758 986 453 116
Fragments arranged by size:
986 758 453 116
SmaI CCC'GGG
Cuts at: 0 384 2313
Size: 384 1929
SmlI C'TyrA_G
Cuts at: 0 970 1783 2313
Size: 970 813 530
Fragments arranged by size:
970 813 530
StuI AGG'CCT
Cuts at: 0 1111 1677 2313
Size: 1111 566 636
Fragments arranged by size:
1111 636 566
StyI C'CwwG_G
Cuts at: 0 204 216 2063 2313
Size: 204 12 1847 250
Fragments arranged by size:
1847 250 204 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 1570 2054 2313
Size: 472 1098 484 259
Fragments arranged by size:
1098 484 472 259
TatI w'GTAC_w
Cuts at: 0 461 1670 2313
Size: 461 1209 643
Fragments arranged by size:
1209 643 461
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 533 565 1584 1616 2313
Size: 90 32 411 32 1019 32 697
Fragments arranged by size:
1019 697 411 90 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1324 2313
Size: 1324 989
XhoI C'TCGA_G
Cuts at: 0 970 2313
Size: 970 1343
XmaI C'CCGG_G
Cuts at: 0 382 2313
Size: 382 1931
XmnI GAAnn'nnTTC
Cuts at: 0 1111 1621 1921 2313
Size: 1111 510 300 392
Fragments arranged by size:
1111 510 392 300
Enzymes that do cut:
AcuI AfeI AflII AgeI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SexAI
SfcI SfiI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AhdI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI ZraI