Restriction map for
pF1KB8570
(Linear) (Six-base) MAPSORT of: KIBB8570.seq Check: 8636 from: 1 to: 2469
KIBB8570.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 62 620 2469
Size: 62 558 1849
Fragments arranged by size:
1849 558 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1085 1318 2469
Size: 1037 48 233 1151
Fragments arranged by size:
1151 1037 233 48
AfeI AGC'GCT
Cuts at: 0 151 2469
Size: 151 2318
AflII C'TTAA_G
Cuts at: 0 655 1627 2469
Size: 655 972 842
Fragments arranged by size:
972 842 655
AflIII A'CryG_T
Cuts at: 0 686 2469
Size: 686 1783
AleI CACnn'nnGTG
Cuts at: 0 1018 2469
Size: 1018 1451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 2469
Size: 221 2248
ApaLI G'TGCA_C
Cuts at: 0 467 2469
Size: 467 2002
ApoI r'AATT_y
Cuts at: 0 547 737 749 2008 2469
Size: 547 190 12 1259 461
Fragments arranged by size:
1259 547 461 190 12
AvaI C'yCGr_G
Cuts at: 0 904 2469
Size: 904 1565
BanI G'GyrC_C
Cuts at: 0 367 2469
Size: 367 2102
BanII G_rGCy'C
Cuts at: 0 49 2469
Size: 49 2420
BbeI G_GCGC'C
Cuts at: 0 371 2469
Size: 371 2098
BbsI GAAGACnn'nnnn_
Cuts at: 0 666 2469
Size: 666 1803
BbvCI CC'TCA_GC
Cuts at: 0 1277 2469
Size: 1277 1192
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 1283 2469
Size: 70 1213 1186
Fragments arranged by size:
1213 1186 70
BclI T'GATC_A
Cuts at: 0 2367 2469
Size: 2367 102
BglI GCCn_nnn'nGGC
Cuts at: 0 29 2469
Size: 29 2440
Bme1580I G_kGCm'C
Cuts at: 0 471 2469
Size: 471 1998
BmgBI CAC'GTC
Cuts at: 0 60 2469
Size: 60 2409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 2068 2469
Size: 453 1615 401
Fragments arranged by size:
1615 453 401
Bpu10I CC'TnA_GC
Cuts at: 0 1277 2095 2469
Size: 1277 818 374
Fragments arranged by size:
1277 818 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1726 2469
Size: 665 1061 743
Fragments arranged by size:
1061 743 665
BsaI GGTCTCn'nnnn_
Cuts at: 0 167 2469
Size: 167 2302
BsaHI Gr'CG_yC
Cuts at: 0 134 182 368 2469
Size: 134 48 186 2101
Fragments arranged by size:
2101 186 134 48
BsaWI w'CCGG_w
Cuts at: 0 73 1715 2469
Size: 73 1642 754
Fragments arranged by size:
1642 754 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2405 2435 2469
Size: 2405 30 34
Fragments arranged by size:
2405 34 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2429 2469
Size: 2429 40
BsiHKAI G_wGCw'C
Cuts at: 0 436 471 2469
Size: 436 35 1998
Fragments arranged by size:
1998 436 35
BsmI GAATG_Cn'
Cuts at: 0 1711 2469
Size: 1711 758
BsmBI CGTCTCn'nnnn_
Cuts at: 0 588 2469
Size: 588 1881
Bsp1286I G_dGCh'C
Cuts at: 0 49 436 471 2469
Size: 49 387 35 1998
Fragments arranged by size:
1998 387 49 35
BspEI T'CCGG_A
Cuts at: 0 73 2469
Size: 73 2396
BspHI T'CATG_A
Cuts at: 0 1509 2469
Size: 1509 960
BsrBI CCG'CTC
Cuts at: 0 170 2469
Size: 170 2299
BsrDI GCAATG_nn'
Cuts at: 0 324 850 2469
Size: 324 526 1619
Fragments arranged by size:
1619 526 324
BsrGI T'GTAC_A
Cuts at: 0 1062 1364 1585 1923 2082 2232 2469
Size: 1062 302 221 338 159 150 237
Fragments arranged by size:
1062 338 302 237 221 159 150
BssHII G'CGCG_C
Cuts at: 0 160 2469
Size: 160 2309
BssSI C'ACGA_G
Cuts at: 0 1910 2326 2469
Size: 1910 416 143
Fragments arranged by size:
1910 416 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1561 2469
Size: 1561 908
BstYI r'GATC_y
Cuts at: 0 107 931 1026 2469
Size: 107 824 95 1443
Fragments arranged by size:
1443 824 107 95
BstZ17I GTA'TAC
Cuts at: 0 621 2469
Size: 621 1848
BtgI C'CryG_G
Cuts at: 0 137 462 2469
Size: 137 325 2007
Fragments arranged by size:
2007 325 137
BtsI GCAGTG_nn'
Cuts at: 0 401 901 2469
Size: 401 500 1568
Fragments arranged by size:
1568 500 401
DraI TTT'AAA
Cuts at: 0 2375 2469
Size: 2375 94
EaeI y'GGCC_r
Cuts at: 0 231 2469
Size: 231 2238
EarI CTCTTCn'nnn_
Cuts at: 0 329 1103 1298 2186 2330 2469
Size: 329 774 195 888 144 139
Fragments arranged by size:
888 774 329 195 144 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1085 1318 2469
Size: 1037 48 233 1151
Fragments arranged by size:
1151 1037 233 48
EcoO109I rG'GnC_Cy
Cuts at: 0 979 2469
Size: 979 1490
EcoRV GAT'ATC
Cuts at: 0 390 2469
Size: 390 2079
HaeII r_GCGC'y
Cuts at: 0 153 371 2469
Size: 153 218 2098
Fragments arranged by size:
2098 218 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 898 930 1204 1236 1450 1482
Size: 68 32 798 32 274 32 214 32
Cuts at: 1482 1804 1836 2187 2219 2469
Size: 322 32 351 32 250
Fragments arranged by size:
798 351 322 274 250 214 68 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 261 2469
Size: 261 2208
HpaI GTT'AAC
Cuts at: 0 261 2469
Size: 261 2208
KasI G'GCGC_C
Cuts at: 0 367 2469
Size: 367 2102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 2235 2469
Size: 777 1458 234
Fragments arranged by size:
1458 777 234
MscI TGG'CCA
Cuts at: 0 233 2469
Size: 233 2236
MslI CAynn'nnrTG
Cuts at: 0 1018 1508 2469
Size: 1018 490 961
Fragments arranged by size:
1018 961 490
MspA1I CmG'CkG
Cuts at: 0 121 139 2469
Size: 121 18 2330
Fragments arranged by size:
2330 121 18
NarI GG'CG_CC
Cuts at: 0 368 2469
Size: 368 2101
NcoI C'CATG_G
Cuts at: 0 462 2469
Size: 462 2007
NspI r_CATG'y
Cuts at: 0 580 690 2469
Size: 580 110 1779
Fragments arranged by size:
1779 580 110
PasI CC'CwG_GG
Cuts at: 0 42 2469
Size: 42 2427
PciI A'CATG_T
Cuts at: 0 686 2469
Size: 686 1783
PfoI T'CCnGG_A
Cuts at: 0 86 2469
Size: 86 2383
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1073 1105 2469
Size: 1073 32 1364
Fragments arranged by size:
1364 1073 32
PstI C_TGCA'G
Cuts at: 0 104 819 2469
Size: 104 715 1650
Fragments arranged by size:
1650 715 104
PvuII CAG'CTG
Cuts at: 0 121 2469
Size: 121 2348
SacII CC_GC'GG
Cuts at: 0 140 2469
Size: 140 2329
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 1298 2469
Size: 1103 195 1171
Fragments arranged by size:
1171 1103 195
SexAI A'CCwGG_T
Cuts at: 0 633 2469
Size: 633 1836
SfcI C'TryA_G
Cuts at: 0 100 815 2469
Size: 100 715 1654
Fragments arranged by size:
1654 715 100
SfoI GGC'GCC
Cuts at: 0 369 2469
Size: 369 2100
SmlI C'TyrA_G
Cuts at: 0 655 680 1627 1741 2469
Size: 655 25 947 114 728
Fragments arranged by size:
947 728 655 114 25
SspI AAT'ATT
Cuts at: 0 666 2443 2469
Size: 666 1777 26
Fragments arranged by size:
1777 666 26
StyI C'CwwG_G
Cuts at: 0 462 2469
Size: 462 2007
TatI w'GTAC_w
Cuts at: 0 1047 1062 1216 1364 1585 1923 2082 2232
Size: 1047 15 154 148 221 338 159 150
Cuts at: 2232 2469
Size: 237
Fragments arranged by size:
1047 338 237 221 159 154 150 148
15
Tth111I GACn'n_nGTC
Cuts at: 0 58 2469
Size: 58 2411
XbaI T'CTAG_A
Cuts at: 0 1831 2455 2469
Size: 1831 624 14
Fragments arranged by size:
1831 624 14
XmnI GAAnn'nnTTC
Cuts at: 0 7 2469
Size: 7 2462
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AleI AlfI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI Bme1580I BmgBI BplI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstYI BstZ17I BtgI BtsI DraI EaeI EarI Eco57MI
EcoO109I EcoRV HaeII Hin4I HincII HpaI KasI MmeI
MscI MslI MspA1I NarI NcoI NspI PasI PciI
PfoI PsrI PstI PvuII SacII SapI SexAI SfcI
SfoI SmlI SspI StyI TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AloI AlwNI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BfrBI BglII BlpI BmrI BmtI BpmI BsaAI BsaBI
BseYI BsgI BsiEI BsiWI BspMI BsrFI BstBI BstEII
BstXI Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HindIII KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
XcmI XhoI XmaI ZraI