Restriction map for
pF1KB8571
(Linear) (Six-base) MAPSORT of: KIBB8571.seq Check: 3391 from: 1 to: 1719
KIBB8571.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 1617 1719
Size: 1617 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1719
Size: 482 1237
AflIII A'CryG_T
Cuts at: 0 916 1708 1719
Size: 916 792 11
Fragments arranged by size:
916 792 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1582 1719
Size: 1582 137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 811 843 1719
Size: 811 32 876
Fragments arranged by size:
876 811 32
AlwNI CAG_nnn'CTG
Cuts at: 0 505 802 1351 1719
Size: 505 297 549 368
Fragments arranged by size:
549 505 368 297
ApoI r'AATT_y
Cuts at: 0 231 1719
Size: 231 1488
AseI AT'TA_AT
Cuts at: 0 1207 1719
Size: 1207 512
AvaI C'yCGr_G
Cuts at: 0 1219 1719
Size: 1219 500
BanI G'GyrC_C
Cuts at: 0 1280 1719
Size: 1280 439
BanII G_rGCy'C
Cuts at: 0 856 1244 1291 1327 1403 1719
Size: 856 388 47 36 76 316
Fragments arranged by size:
856 388 316 76 47 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 1366 1687 1719
Size: 277 1089 321 32
Fragments arranged by size:
1089 321 277 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 1573 1719
Size: 1573 146
BclI T'GATC_A
Cuts at: 0 111 1719
Size: 111 1608
BfrBI ATG'CAT
Cuts at: 0 1667 1719
Size: 1667 52
BmgBI CAC'GTC
Cuts at: 0 917 1719
Size: 917 802
BmrI ACTGGGnnnn_n'
Cuts at: 0 1286 1697 1719
Size: 1286 411 22
Fragments arranged by size:
1286 411 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 1719
Size: 453 1266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 794 1258 1405 1623 1719
Size: 527 267 464 147 218 96
Fragments arranged by size:
527 464 267 218 147 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1719
Size: 1022 697
BsaAI yAC'GTr
Cuts at: 0 151 1719
Size: 151 1568
BsaBI GATnn'nnATC
Cuts at: 0 63 1719
Size: 63 1656
BsaHI Gr'CG_yC
Cuts at: 0 1614 1719
Size: 1614 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 792 822 1644 1674 1719
Size: 792 30 822 30 45
Fragments arranged by size:
822 792 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1307 1719
Size: 1307 412
BseYI C'CCAG_C
Cuts at: 0 1304 1719
Size: 1304 415
BsiHKAI G_wGCw'C
Cuts at: 0 856 1403 1654 1719
Size: 856 547 251 65
Fragments arranged by size:
856 547 251 65
BsmI GAATG_Cn'
Cuts at: 0 1461 1719
Size: 1461 258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 907 1072 1719
Size: 907 165 647
Fragments arranged by size:
907 647 165
Bsp1286I G_dGCh'C
Cuts at: 0 856 1244 1291 1327 1403 1654 1719
Size: 856 388 47 36 76 251 65
Fragments arranged by size:
856 388 251 76 65 47 36
BspHI T'CATG_A
Cuts at: 0 980 1719
Size: 980 739
BsrBI CCG'CTC
Cuts at: 0 1700 1719
Size: 1700 19
BsrDI GCAATG_nn'
Cuts at: 0 747 1466 1719
Size: 747 719 253
Fragments arranged by size:
747 719 253
BsrGI T'GTAC_A
Cuts at: 0 588 1719
Size: 588 1131
BstEII G'GTnAC_C
Cuts at: 0 1411 1719
Size: 1411 308
BtgI C'CryG_G
Cuts at: 0 987 1719
Size: 987 732
BtsI GCAGTG_nn'
Cuts at: 0 280 1719
Size: 280 1439
DraI TTT'AAA
Cuts at: 0 54 1719
Size: 54 1665
DraIII CAC_nnn'GTG
Cuts at: 0 1280 1719
Size: 1280 439
EarI CTCTTCn'nnn_
Cuts at: 0 1370 1537 1576 1642 1719
Size: 1370 167 39 66 77
Fragments arranged by size:
1370 167 77 66 39
EcoICRI GAG'CTC
Cuts at: 0 854 1401 1719
Size: 854 547 318
Fragments arranged by size:
854 547 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 527 794 1258 1405 1623 1719
Size: 482 45 267 464 147 218 96
Fragments arranged by size:
482 464 267 218 147 96 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 473 1719
Size: 473 1246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 757 789 1231 1263 1571 1603 1684
Size: 26 731 32 442 32 308 32 81
Cuts at: 1684 1716 1719
Size: 32 3
Fragments arranged by size:
731 442 308 81 32 32 32 32
26 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1432 1719
Size: 372 1060 287
Fragments arranged by size:
1060 372 287
MslI CAynn'nnrTG
Cuts at: 0 992 1184 1564 1672 1719
Size: 992 192 380 108 47
Fragments arranged by size:
992 380 192 108 47
MspA1I CmG'CkG
Cuts at: 0 289 1348 1373 1719
Size: 289 1059 25 346
Fragments arranged by size:
1059 346 289 25
NcoI C'CATG_G
Cuts at: 0 987 1719
Size: 987 732
NsiI A_TGCA'T
Cuts at: 0 1669 1719
Size: 1669 50
NspI r_CATG'y
Cuts at: 0 1712 1719
Size: 1712 7
PacI TTA_AT'TAA
Cuts at: 0 188 1719
Size: 188 1531
PasI CC'CwG_GG
Cuts at: 0 903 1491 1719
Size: 903 588 228
Fragments arranged by size:
903 588 228
PciI A'CATG_T
Cuts at: 0 1708 1719
Size: 1708 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 876 1669 1719
Size: 292 584 793 50
Fragments arranged by size:
793 584 292 50
PmlI CAC'GTG
Cuts at: 0 151 1719
Size: 151 1568
PstI C_TGCA'G
Cuts at: 0 1347 1719
Size: 1347 372
PvuII CAG'CTG
Cuts at: 0 289 1348 1373 1719
Size: 289 1059 25 346
Fragments arranged by size:
1059 346 289 25
SacI G_AGCT'C
Cuts at: 0 856 1403 1719
Size: 856 547 316
Fragments arranged by size:
856 547 316
SapI GCTCTTCn'nnn_
Cuts at: 0 1576 1642 1719
Size: 1576 66 77
Fragments arranged by size:
1576 77 66
SfcI C'TryA_G
Cuts at: 0 1343 1719
Size: 1343 376
SmlI C'TyrA_G
Cuts at: 0 1037 1719
Size: 1037 682
SspI AAT'ATT
Cuts at: 0 565 1719
Size: 565 1154
StyI C'CwwG_G
Cuts at: 0 407 987 1416 1719
Size: 407 580 429 303
Fragments arranged by size:
580 429 407 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 1268 1719
Size: 30 1238 451
Fragments arranged by size:
1238 451 30
TatI w'GTAC_w
Cuts at: 0 588 1719
Size: 588 1131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1412 1444 1644 1676 1719
Size: 1412 32 200 32 43
Fragments arranged by size:
1412 200 43 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 564 1719
Size: 564 1155
ZraI GAC'GTC
Cuts at: 0 1615 1719
Size: 1615 104
Enzymes that do cut:
AatII AcuI AflIII AlfI AloI AlwNI ApoI AseI
AvaI BanI BanII BbsI BciVI BclI BfrBI BmgBI
BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI
BsrDI BsrGI BstEII BtgI BtsI DraI DraIII EarI
EcoICRI Eco57MI EcoNI Hin4I MmeI MslI MspA1I NcoI
NsiI NspI PacI PasI PciI PflMI PmlI PstI
PvuII SacI SapI SfcI SmlI SspI StyI TaqII
TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmtI
Bpu10I BsaI BsaWI BsgI BsiEI BsiWI BspEI BspMI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DrdI EaeI EagI EciI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI