Restriction map for
pF1KB8574
(Linear) (Six-base) MAPSORT of: KIBB8574.seq Check: 1425 from: 1 to: 2343
KIBB8574.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 795 1794 2343
Size: 795 999 549
Fragments arranged by size:
999 795 549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1396 1592 1852 2013 2343
Size: 827 569 196 260 161 330
Fragments arranged by size:
827 569 330 260 196 161
AhdI GACnn_n'nnGTC
Cuts at: 0 1790 2343
Size: 1790 553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1702 2343
Size: 1702 641
AlwNI CAG_nnn'CTG
Cuts at: 0 100 2008 2130 2343
Size: 100 1908 122 213
Fragments arranged by size:
1908 213 122 100
ApaI G_GGCC'C
Cuts at: 0 2046 2182 2343
Size: 2046 136 161
Fragments arranged by size:
2046 161 136
ApoI r'AATT_y
Cuts at: 0 430 1003 1057 2331 2337 2343
Size: 430 573 54 1274 6 6
Fragments arranged by size:
1274 573 430 54 6 6
AvaI C'yCGr_G
Cuts at: 0 1498 2343
Size: 1498 845
BamHI G'GATC_C
Cuts at: 0 822 2343
Size: 822 1521
BanI G'GyrC_C
Cuts at: 0 2154 2343
Size: 2154 189
BanII G_rGCy'C
Cuts at: 0 1595 2046 2144 2182 2343
Size: 1595 451 98 38 161
Fragments arranged by size:
1595 451 161 98 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 1291 2343
Size: 19 1272 1052
Fragments arranged by size:
1272 1052 19
BclI T'GATC_A
Cuts at: 0 243 2343
Size: 243 2100
BglI GCCn_nnn'nGGC
Cuts at: 0 2092 2343
Size: 2092 251
Bme1580I G_kGCm'C
Cuts at: 0 2046 2182 2343
Size: 2046 136 161
Fragments arranged by size:
2046 161 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 122 2343
Size: 102 20 2221
Fragments arranged by size:
2221 102 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1293 1579 2131 2308 2343
Size: 1177 116 286 552 177 35
Fragments arranged by size:
1177 552 286 177 116 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 2343
Size: 897 1446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 627 657 2343
Size: 627 30 1686
Fragments arranged by size:
1686 627 30
BseYI C'CCAG_C
Cuts at: 0 32 2161 2182 2208 2343
Size: 32 2129 21 26 135
Fragments arranged by size:
2129 135 32 26 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1144 2140 2343
Size: 485 659 996 203
Fragments arranged by size:
996 659 485 203
BsiHKAI G_wGCw'C
Cuts at: 0 1595 2343
Size: 1595 748
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1423 2343
Size: 1423 920
Bsp1286I G_dGCh'C
Cuts at: 0 1595 2046 2144 2182 2343
Size: 1595 451 98 38 161
Fragments arranged by size:
1595 451 161 98 38
BsrDI GCAATG_nn'
Cuts at: 0 392 761 2343
Size: 392 369 1582
Fragments arranged by size:
1582 392 369
BsrGI T'GTAC_A
Cuts at: 0 652 2343
Size: 652 1691
BstEII G'GTnAC_C
Cuts at: 0 358 2343
Size: 358 1985
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1203 2343
Size: 1203 1140
BstYI r'GATC_y
Cuts at: 0 777 822 1346 1649 1767 2024 2343
Size: 777 45 524 303 118 257 319
Fragments arranged by size:
777 524 319 303 257 118 45
BstZ17I GTA'TAC
Cuts at: 0 796 2343
Size: 796 1547
Bsu36I CC'TnA_GG
Cuts at: 0 2150 2343
Size: 2150 193
BtsI GCAGTG_nn'
Cuts at: 0 575 1229 2343
Size: 575 654 1114
Fragments arranged by size:
1114 654 575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 484 519 2343
Size: 484 35 1824
Fragments arranged by size:
1824 484 35
DraI TTT'AAA
Cuts at: 0 1092 2343
Size: 1092 1251
EarI CTCTTCn'nnn_
Cuts at: 0 545 1029 1102 1568 2222 2343
Size: 545 484 73 466 654 121
Fragments arranged by size:
654 545 484 466 121 73
EcoICRI GAG'CTC
Cuts at: 0 1593 2343
Size: 1593 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1177 1293 1396 1579 1592 1852 2013
Size: 827 350 116 103 183 13 260 161
Cuts at: 2013 2131 2308 2343
Size: 118 177 35
Fragments arranged by size:
827 350 260 183 177 161 118 116
103 35 13
EcoO109I rG'GnC_Cy
Cuts at: 0 508 2042 2178 2179 2343
Size: 508 1534 136 1 164
Fragments arranged by size:
1534 508 164 136 1
HaeII r_GCGC'y
Cuts at: 0 1316 2343
Size: 1316 1027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 367 399 1533 1552 1565 1584
Size: 283 32 52 32 1134 19 13 19
Cuts at: 1584 1776 1808 2343
Size: 192 32 535
Fragments arranged by size:
1134 535 283 192 52 32 32 32
19 19 13
HincII GTy'rAC
Cuts at: 0 1795 2343
Size: 1795 548
HindIII A'AGCT_T
Cuts at: 0 809 2343
Size: 809 1534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 1197 2343
Size: 1109 88 1146
Fragments arranged by size:
1146 1109 88
MslI CAynn'nnrTG
Cuts at: 0 164 2343
Size: 164 2179
MspA1I CmG'CkG
Cuts at: 0 565 2186 2343
Size: 565 1621 157
Fragments arranged by size:
1621 565 157
NspI r_CATG'y
Cuts at: 0 24 179 1146 1183 2343
Size: 24 155 967 37 1160
Fragments arranged by size:
1160 967 155 37 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 2064 2343
Size: 2064 279
PpuMI rG'GwC_Cy
Cuts at: 0 508 2343
Size: 508 1835
PsiI TTA'TAA
Cuts at: 0 119 2343
Size: 119 2224
PspOMI G'GGCC_C
Cuts at: 0 2042 2178 2343
Size: 2042 136 165
Fragments arranged by size:
2042 165 136
PstI C_TGCA'G
Cuts at: 0 43 570 1681 2196 2343
Size: 43 527 1111 515 147
Fragments arranged by size:
1111 527 515 147 43
PvuII CAG'CTG
Cuts at: 0 565 2186 2343
Size: 565 1621 157
Fragments arranged by size:
1621 565 157
SacI G_AGCT'C
Cuts at: 0 1595 2343
Size: 1595 748
SalI G'TCGA_C
Cuts at: 0 1793 2343
Size: 1793 550
SapI GCTCTTCn'nnn_
Cuts at: 0 1102 1568 2222 2343
Size: 1102 466 654 121
Fragments arranged by size:
1102 654 466 121
SfcI C'TryA_G
Cuts at: 0 39 566 707 1677 2192 2343
Size: 39 527 141 970 515 151
Fragments arranged by size:
970 527 515 151 141 39
SmlI C'TyrA_G
Cuts at: 0 912 2343
Size: 912 1431
SphI G_CATG'C
Cuts at: 0 1146 1183 2343
Size: 1146 37 1160
Fragments arranged by size:
1160 1146 37
StuI AGG'CCT
Cuts at: 0 1612 2148 2343
Size: 1612 536 195
Fragments arranged by size:
1612 536 195
StyI C'CwwG_G
Cuts at: 0 982 1301 1352 2303 2343
Size: 982 319 51 951 40
Fragments arranged by size:
982 951 319 51 40
TatI w'GTAC_w
Cuts at: 0 652 2343
Size: 652 1691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 2343
Size: 37 32 2274
Fragments arranged by size:
2274 37 32
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BclI BglI Bme1580I BmrI
BpmI BpuEI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrDI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtsI
CspCI DraI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII MmeI MslI MspA1I NspI PflMI PpuMI
PsiI PspOMI PstI PvuII SacI SalI SapI SfcI
SmlI SphI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BtgI BtgZI ClaI
DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI