Restriction map for
pF1KB8579
(Linear) (Six-base) MAPSORT of: KIBB8579.seq Check: 3812 from: 1 to: 696
KIBB8579.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AatII G_ACGT'C
Cuts at: 0 446 696
Size: 446 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 696
Size: 594 102
AhdI GACnn_n'nnGTC
Cuts at: 0 162 696
Size: 162 534
AleI CACnn'nnGTG
Cuts at: 0 275 696
Size: 275 421
AlwNI CAG_nnn'CTG
Cuts at: 0 297 573 621 696
Size: 297 276 48 75
Fragments arranged by size:
297 276 75 48
AvaI C'yCGr_G
Cuts at: 0 113 696
Size: 113 583
BanI G'GyrC_C
Cuts at: 0 507 696
Size: 507 189
BanII G_rGCy'C
Cuts at: 0 154 331 696
Size: 154 177 365
Fragments arranged by size:
365 177 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 696
Size: 209 487
BglI GCCn_nnn'nGGC
Cuts at: 0 380 696
Size: 380 316
BlpI GC'TnA_GC
Cuts at: 0 93 628 696
Size: 93 535 68
Fragments arranged by size:
535 93 68
Bme1580I G_kGCm'C
Cuts at: 0 512 696
Size: 512 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 696
Size: 166 530
BsaHI Gr'CG_yC
Cuts at: 0 336 443 696
Size: 336 107 253
Fragments arranged by size:
336 253 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 696
Size: 109 30 557
Fragments arranged by size:
557 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 133 696
Size: 30 103 563
Fragments arranged by size:
563 103 30
BseYI C'CCAG_C
Cuts at: 0 324 499 696
Size: 324 175 197
Fragments arranged by size:
324 197 175
BsiEI CG_ry'CG
Cuts at: 0 85 696
Size: 85 611
BsiHKAI G_wGCw'C
Cuts at: 0 154 634 696
Size: 154 480 62
Fragments arranged by size:
480 154 62
Bsp1286I G_dGCh'C
Cuts at: 0 154 331 512 634 696
Size: 154 177 181 122 62
Fragments arranged by size:
181 177 154 122 62
BsrBI CCG'CTC
Cuts at: 0 134 696
Size: 134 562
BsrGI T'GTAC_A
Cuts at: 0 662 696
Size: 662 34
BssHII G'CGCG_C
Cuts at: 0 343 696
Size: 343 353
BssSI C'ACGA_G
Cuts at: 0 138 529 696
Size: 138 391 167
Fragments arranged by size:
391 167 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 467 696
Size: 297 170 229
Fragments arranged by size:
297 229 170
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 696
Size: 144 552
BstYI r'GATC_y
Cuts at: 0 563 696
Size: 563 133
Bsu36I CC'TnA_GG
Cuts at: 0 260 696
Size: 260 436
DraIII CAC_nnn'GTG
Cuts at: 0 477 696
Size: 477 219
EaeI y'GGCC_r
Cuts at: 0 82 696
Size: 82 614
EagI C'GGCC_G
Cuts at: 0 82 696
Size: 82 614
EarI CTCTTCn'nnn_
Cuts at: 0 48 696
Size: 48 648
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 573 696
Size: 573 123
EcoICRI GAG'CTC
Cuts at: 0 152 696
Size: 152 544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 594 696
Size: 166 428 102
Fragments arranged by size:
428 166 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 696
Size: 313 383
HaeII r_GCGC'y
Cuts at: 0 436 696
Size: 436 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 148 180 211 243 696
Size: 7 32 109 32 31 32 453
Fragments arranged by size:
453 109 32 32 32 31 7
MslI CAynn'nnrTG
Cuts at: 0 275 696
Size: 275 421
MspA1I CmG'CkG
Cuts at: 0 294 486 522 678 696
Size: 294 192 36 156 18
Fragments arranged by size:
294 192 156 36 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 696
Size: 477 219
PstI C_TGCA'G
Cuts at: 0 302 696
Size: 302 394
PvuII CAG'CTG
Cuts at: 0 294 486 522 678 696
Size: 294 192 36 156 18
Fragments arranged by size:
294 192 156 36 18
SacI G_AGCT'C
Cuts at: 0 154 696
Size: 154 542
SfcI C'TryA_G
Cuts at: 0 298 405 696
Size: 298 107 291
Fragments arranged by size:
298 291 107
SmaI CCC'GGG
Cuts at: 0 115 696
Size: 115 581
TatI w'GTAC_w
Cuts at: 0 662 696
Size: 662 34
XmaI C'CCGG_G
Cuts at: 0 113 696
Size: 113 583
ZraI GAC'GTC
Cuts at: 0 444 696
Size: 444 252
Enzymes that do cut:
AatII AcuI AhdI AleI AlwNI AvaI BanI BanII
BbsI BglI BlpI Bme1580I BpmI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI HaeII Hin4I MslI MspA1I
PflMI PstI PvuII SacI SfcI SmaI TatI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BstBI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmnI