Restriction map for
pF1KB8586
(Linear) (Six-base) MAPSORT of: KIBB8586.seq Check: 294 from: 1 to: 918
KIBB8586.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AflIII A'CryG_T
Cuts at: 0 765 918
Size: 765 153
AleI CACnn'nnGTG
Cuts at: 0 784 918
Size: 784 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 918
Size: 565 32 321
Fragments arranged by size:
565 321 32
AlwNI CAG_nnn'CTG
Cuts at: 0 901 918
Size: 901 17
ApoI r'AATT_y
Cuts at: 0 31 469 918
Size: 31 438 449
Fragments arranged by size:
449 438 31
AvaI C'yCGr_G
Cuts at: 0 313 918
Size: 313 605
BamHI G'GATC_C
Cuts at: 0 93 124 918
Size: 93 31 794
Fragments arranged by size:
794 93 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 918
Size: 454 464
BglII A'GATC_T
Cuts at: 0 354 918
Size: 354 564
Bme1580I G_kGCm'C
Cuts at: 0 467 918
Size: 467 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 175 918
Size: 175 743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 918
Size: 616 302
Bpu10I CC'TnA_GC
Cuts at: 0 138 918
Size: 138 780
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 589 595 619 867 897 918
Size: 565 24 6 24 248 30 21
Fragments arranged by size:
565 248 30 24 24 21 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 585 918
Size: 585 333
Bsp1286I G_dGCh'C
Cuts at: 0 467 918
Size: 467 451
BspHI T'CATG_A
Cuts at: 0 692 918
Size: 692 226
BsrDI GCAATG_nn'
Cuts at: 0 45 734 918
Size: 45 689 184
Fragments arranged by size:
689 184 45
BstYI r'GATC_y
Cuts at: 0 93 124 295 354 715 918
Size: 93 31 171 59 361 203
Fragments arranged by size:
361 203 171 93 59 31
EaeI y'GGCC_r
Cuts at: 0 739 776 918
Size: 739 37 142
Fragments arranged by size:
739 142 37
EarI CTCTTCn'nnn_
Cuts at: 0 138 631 918
Size: 138 493 287
Fragments arranged by size:
493 287 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 918
Size: 616 302
EcoRI G'AATT_C
Cuts at: 0 469 918
Size: 469 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 316 918
Size: 316 602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 608 640 846 878 918
Size: 608 32 206 32 40
Fragments arranged by size:
608 206 40 32 32
MfeI C'AATT_G
Cuts at: 0 782 791 918
Size: 782 9 127
Fragments arranged by size:
782 127 9
MluI A'CGCG_T
Cuts at: 0 765 918
Size: 765 153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 661 918
Size: 446 215 257
Fragments arranged by size:
446 257 215
MscI TGG'CCA
Cuts at: 0 741 778 918
Size: 741 37 140
Fragments arranged by size:
741 140 37
MslI CAynn'nnrTG
Cuts at: 0 784 918
Size: 784 134
MspA1I CmG'CkG
Cuts at: 0 745 918
Size: 745 173
NdeI CA'TA_TG
Cuts at: 0 806 918
Size: 806 112
PasI CC'CwG_GG
Cuts at: 0 702 918
Size: 702 216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 918
Size: 565 32 321
Fragments arranged by size:
565 321 32
PstI C_TGCA'G
Cuts at: 0 906 918
Size: 906 12
PvuII CAG'CTG
Cuts at: 0 745 918
Size: 745 173
SexAI A'CCwGG_T
Cuts at: 0 272 918
Size: 272 646
SfcI C'TryA_G
Cuts at: 0 902 918
Size: 902 16
SmaI CCC'GGG
Cuts at: 0 315 918
Size: 315 603
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 589 621 918
Size: 589 32 297
Fragments arranged by size:
589 297 32
XmaI C'CCGG_G
Cuts at: 0 313 918
Size: 313 605
Enzymes that do cut:
AflIII AleI AloI AlwNI ApoI AvaI BamHI BbsI
BglII Bme1580I BmrI BpmI Bpu10I BsaXI BsmBI Bsp1286I
BspHI BsrDI BstYI EaeI EarI Eco57MI EcoRI FalI
Hin4I MfeI MluI MmeI MscI MslI MspA1I NdeI
PasI PpiI PstI PvuII SexAI SfcI SmaI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmnI ZraI