Restriction map for
pF1KB8587
(Linear) (Six-base) MAPSORT of: KIBB8587.seq Check: 4762 from: 1 to: 711
KIBB8587.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 604 711
Size: 248 356 107
Fragments arranged by size:
356 248 107
AhdI GACnn_n'nnGTC
Cuts at: 0 276 711
Size: 276 435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 711
Size: 413 32 266
Fragments arranged by size:
413 266 32
AlwNI CAG_nnn'CTG
Cuts at: 0 533 711
Size: 533 178
ApaI G_GGCC'C
Cuts at: 0 69 711
Size: 69 642
ApoI r'AATT_y
Cuts at: 0 367 423 553 711
Size: 367 56 130 158
Fragments arranged by size:
367 158 130 56
AvaI C'yCGr_G
Cuts at: 0 68 711
Size: 68 643
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 520 553 711
Size: 520 33 158
Fragments arranged by size:
520 158 33
BanII G_rGCy'C
Cuts at: 0 22 69 407 711
Size: 22 47 338 304
Fragments arranged by size:
338 304 47 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 706 711
Size: 706 5
BciVI GTATCCnnnnn_n'
Cuts at: 0 548 711
Size: 548 163
BglI GCCn_nnn'nGGC
Cuts at: 0 100 711
Size: 100 611
BlpI GC'TnA_GC
Cuts at: 0 119 711
Size: 119 592
Bme1580I G_kGCm'C
Cuts at: 0 69 588 711
Size: 69 519 123
Fragments arranged by size:
519 123 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 462 711
Size: 462 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 134 711
Size: 134 577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 163 383 711
Size: 6 157 220 328
Fragments arranged by size:
328 220 157 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 711
Size: 386 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 711
Size: 546 165
BsiHKAI G_wGCw'C
Cuts at: 0 511 711
Size: 511 200
Bsp1286I G_dGCh'C
Cuts at: 0 22 69 407 511 588 711
Size: 22 47 338 104 77 123
Fragments arranged by size:
338 123 104 77 47 22
BspHI T'CATG_A
Cuts at: 0 432 473 711
Size: 432 41 238
Fragments arranged by size:
432 238 41
BsrDI GCAATG_nn'
Cuts at: 0 346 711
Size: 346 365
BtgI C'CryG_G
Cuts at: 0 95 711
Size: 95 616
BtsI GCAGTG_nn'
Cuts at: 0 332 711
Size: 332 379
EarI CTCTTCn'nnn_
Cuts at: 0 661 711
Size: 661 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 163 248 383 604 711
Size: 6 157 85 135 221 107
Fragments arranged by size:
221 157 135 107 85 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 711
Size: 319 392
EcoO109I rG'GnC_Cy
Cuts at: 0 65 232 711
Size: 65 167 479
Fragments arranged by size:
479 167 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 262 294 711
Size: 177 32 53 32 417
Fragments arranged by size:
417 177 53 32 32
MspA1I CmG'CkG
Cuts at: 0 123 711
Size: 123 588
NcoI C'CATG_G
Cuts at: 0 95 711
Size: 95 616
NspI r_CATG'y
Cuts at: 0 279 465 711
Size: 279 186 246
Fragments arranged by size:
279 246 186
PasI CC'CwG_GG
Cuts at: 0 15 711
Size: 15 696
PpuMI rG'GwC_Cy
Cuts at: 0 232 711
Size: 232 479
PsiI TTA'TAA
Cuts at: 0 247 711
Size: 247 464
PspOMI G'GGCC_C
Cuts at: 0 65 711
Size: 65 646
StyI C'CwwG_G
Cuts at: 0 95 320 711
Size: 95 225 391
Fragments arranged by size:
391 225 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 711
Size: 123 588
TatI w'GTAC_w
Cuts at: 0 337 711
Size: 337 374
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApaI ApoI AvaI BaeI
BanII BbsI BciVI BglI BlpI Bme1580I BmrI BplI
BpmI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI BtgI
BtsI EarI Eco57MI EcoNI EcoO109I Hin4I MspA1I NcoI
NspI PasI PpuMI PsiI PspOMI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglII
BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI