Restriction map for
pF1KB8589
(Linear) (Six-base) MAPSORT of: KIBB8589.seq Check: 5887 from: 1 to: 1074
KIBB8589.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AatII G_ACGT'C
Cuts at: 0 483 1074
Size: 483 591
AccI GT'mk_AC
Cuts at: 0 219 1074
Size: 219 855
Acc65I G'GTAC_C
Cuts at: 0 828 1074
Size: 828 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1074
Size: 803 271
AflIII A'CryG_T
Cuts at: 0 124 1074
Size: 124 950
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 1074
Size: 614 32 428
Fragments arranged by size:
614 428 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1066 1074
Size: 1066 8
ApaLI G'TGCA_C
Cuts at: 0 1056 1074
Size: 1056 18
ApoI r'AATT_y
Cuts at: 0 227 1074
Size: 227 847
AvaI C'yCGr_G
Cuts at: 0 232 1074
Size: 232 842
BanI G'GyrC_C
Cuts at: 0 828 1074
Size: 828 246
BanII G_rGCy'C
Cuts at: 0 586 1074
Size: 586 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 638 734 1074
Size: 638 96 340
Fragments arranged by size:
638 340 96
BclI T'GATC_A
Cuts at: 0 21 1074
Size: 21 1053
BfrBI ATG'CAT
Cuts at: 0 310 1074
Size: 310 764
BglII A'GATC_T
Cuts at: 0 1033 1074
Size: 1033 41
Bme1580I G_kGCm'C
Cuts at: 0 1060 1074
Size: 1060 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1074
Size: 262 812
BsaHI Gr'CG_yC
Cuts at: 0 480 1074
Size: 480 594
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 1074
Size: 614 30 430
Fragments arranged by size:
614 430 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1074
Size: 65 1009
BsiEI CG_ry'CG
Cuts at: 0 969 982 1074
Size: 969 13 92
Fragments arranged by size:
969 92 13
BsiHKAI G_wGCw'C
Cuts at: 0 213 586 1060 1074
Size: 213 373 474 14
Fragments arranged by size:
474 373 213 14
Bsp1286I G_dGCh'C
Cuts at: 0 213 586 1060 1074
Size: 213 373 474 14
Fragments arranged by size:
474 373 213 14
BspHI T'CATG_A
Cuts at: 0 804 1074
Size: 804 270
BsrBI CCG'CTC
Cuts at: 0 598 1074
Size: 598 476
BstAPI GCAn_nnn'nTGC
Cuts at: 0 904 1074
Size: 904 170
BstYI r'GATC_y
Cuts at: 0 1033 1074
Size: 1033 41
ClaI AT'CG_AT
Cuts at: 0 969 978 993 1074
Size: 969 9 15 81
Fragments arranged by size:
969 81 15 9
DraIII CAC_nnn'GTG
Cuts at: 0 619 1074
Size: 619 455
EcoICRI GAG'CTC
Cuts at: 0 584 1074
Size: 584 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1074
Size: 803 271
EcoO109I rG'GnC_Cy
Cuts at: 0 656 1074
Size: 656 418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 273 1074
Size: 273 801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 793 825 877 909 1074
Size: 592 32 169 32 52 32 165
Fragments arranged by size:
592 169 165 52 32 32 32
HincII GTy'rAC
Cuts at: 0 220 683 1074
Size: 220 463 391
Fragments arranged by size:
463 391 220
HpaI GTT'AAC
Cuts at: 0 683 1074
Size: 683 391
KpnI G_GTAC'C
Cuts at: 0 832 1074
Size: 832 242
MslI CAynn'nnrTG
Cuts at: 0 406 1074
Size: 406 668
MspA1I CmG'CkG
Cuts at: 0 769 1074
Size: 769 305
NsiI A_TGCA'T
Cuts at: 0 312 1074
Size: 312 762
NspI r_CATG'y
Cuts at: 0 39 128 1074
Size: 39 89 946
Fragments arranged by size:
946 89 39
PciI A'CATG_T
Cuts at: 0 124 1074
Size: 124 950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 614 646 1074
Size: 614 32 428
Fragments arranged by size:
614 428 32
PstI C_TGCA'G
Cuts at: 0 900 1074
Size: 900 174
PvuI CG_AT'CG
Cuts at: 0 969 982 1074
Size: 969 13 92
Fragments arranged by size:
969 92 13
PvuII CAG'CTG
Cuts at: 0 769 1074
Size: 769 305
SacI G_AGCT'C
Cuts at: 0 586 1074
Size: 586 488
SalI G'TCGA_C
Cuts at: 0 218 1074
Size: 218 856
SfcI C'TryA_G
Cuts at: 0 896 1074
Size: 896 178
SmlI C'TyrA_G
Cuts at: 0 277 1074
Size: 277 797
SpeI A'CTAG_T
Cuts at: 0 358 1074
Size: 358 716
StuI AGG'CCT
Cuts at: 0 1044 1074
Size: 1044 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 323 1074
Size: 323 751
XbaI T'CTAG_A
Cuts at: 0 1036 1074
Size: 1036 38
ZraI GAC'GTC
Cuts at: 0 481 1074
Size: 481 593
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AloI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BclI BfrBI BglII
Bme1580I BpuEI BsaHI BsaXI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BsrBI BstAPI BstYI ClaI DraIII EcoICRI Eco57MI
EcoO109I FalI Hin4I HincII HpaI KpnI MslI MspA1I
NsiI NspI PciI PpiI PstI PvuI PvuII SacI
SalI SfcI SmlI SpeI StuI TaqII XbaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BglI BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII KasI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StyI SwaI TatI TstI Tth111I
XcmI XhoI XmaI XmnI