Restriction map for
pF1KB8594
(Linear) (Six-base) MAPSORT of: KIBB8594.seq Check: 3554 from: 1 to: 1440
KIBB8594.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 478 1440
Size: 478 962
AatII G_ACGT'C
Cuts at: 0 198 1440
Size: 198 1242
AccI GT'mk_AC
Cuts at: 0 153 1440
Size: 153 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1440
Size: 152 1288
AflIII A'CryG_T
Cuts at: 0 1228 1440
Size: 1228 212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 1440
Size: 32 1408
ApoI r'AATT_y
Cuts at: 0 93 810 837 919 1073 1261 1440
Size: 93 717 27 82 154 188 179
Fragments arranged by size:
717 188 179 154 93 82 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 279 312 1440
Size: 279 33 1128
Fragments arranged by size:
1128 279 33
BanII G_rGCy'C
Cuts at: 0 528 1440
Size: 528 912
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 1118 1214 1440
Size: 398 720 96 226
Fragments arranged by size:
720 398 226 96
BbvCI CC'TCA_GC
Cuts at: 0 422 1440
Size: 422 1018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 1136 1440
Size: 1102 34 304
Fragments arranged by size:
1102 304 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 280 1440
Size: 280 1160
BglI GCCn_nnn'nGGC
Cuts at: 0 980 1440
Size: 980 460
BmrI ACTGGGnnnn_n'
Cuts at: 0 580 1440
Size: 580 860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 1440
Size: 5 1435
Bpu10I CC'TnA_GC
Cuts at: 0 422 1440
Size: 422 1018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1186 1440
Size: 1064 122 254
Fragments arranged by size:
1064 254 122
BsaHI Gr'CG_yC
Cuts at: 0 195 1440
Size: 195 1245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1218 1248 1440
Size: 1218 30 192
Fragments arranged by size:
1218 192 30
BseYI C'CCAG_C
Cuts at: 0 1109 1440
Size: 1109 331
BsiHKAI G_wGCw'C
Cuts at: 0 528 1236 1440
Size: 528 708 204
Fragments arranged by size:
708 528 204
BsmI GAATG_Cn'
Cuts at: 0 105 902 1440
Size: 105 797 538
Fragments arranged by size:
797 538 105
Bsp1286I G_dGCh'C
Cuts at: 0 528 1236 1440
Size: 528 708 204
Fragments arranged by size:
708 528 204
BspHI T'CATG_A
Cuts at: 0 738 1440
Size: 738 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 478 672 1440
Size: 478 194 768
Fragments arranged by size:
768 478 194
BsrDI GCAATG_nn'
Cuts at: 0 1098 1440
Size: 1098 342
BtgI C'CryG_G
Cuts at: 0 291 1354 1440
Size: 291 1063 86
Fragments arranged by size:
1063 291 86
BtsI GCAGTG_nn'
Cuts at: 0 1419 1440
Size: 1419 21
DraI TTT'AAA
Cuts at: 0 440 777 1440
Size: 440 337 663
Fragments arranged by size:
663 440 337
EarI CTCTTCn'nnn_
Cuts at: 0 171 849 907 947 1440
Size: 171 678 58 40 493
Fragments arranged by size:
678 493 171 58 40
EcoICRI GAG'CTC
Cuts at: 0 526 1440
Size: 526 914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 152 1440
Size: 5 147 1288
Fragments arranged by size:
1288 147 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 203 1440
Size: 203 1237
EcoO109I rG'GnC_Cy
Cuts at: 0 63 1440
Size: 63 1377
EcoRI G'AATT_C
Cuts at: 0 1261 1440
Size: 1261 179
FspI TGC'GCA
Cuts at: 0 901 1440
Size: 901 539
FspAI rTGC'GCAy
Cuts at: 0 901 1440
Size: 901 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 418 450 985 1017 1440
Size: 117 32 269 32 535 32 423
Fragments arranged by size:
535 423 269 117 32 32 32
HincII GTy'rAC
Cuts at: 0 154 855 1440
Size: 154 701 585
Fragments arranged by size:
701 585 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1440
Size: 702 738
MslI CAynn'nnrTG
Cuts at: 0 281 965 1440
Size: 281 684 475
Fragments arranged by size:
684 475 281
NcoI C'CATG_G
Cuts at: 0 291 1440
Size: 291 1149
NspI r_CATG'y
Cuts at: 0 1232 1440
Size: 1232 208
PciI A'CATG_T
Cuts at: 0 1228 1440
Size: 1228 212
PpuMI rG'GwC_Cy
Cuts at: 0 63 1440
Size: 63 1377
PsiI TTA'TAA
Cuts at: 0 644 1440
Size: 644 796
PstI C_TGCA'G
Cuts at: 0 489 1440
Size: 489 951
SacI G_AGCT'C
Cuts at: 0 528 1440
Size: 528 912
SalI G'TCGA_C
Cuts at: 0 152 1440
Size: 152 1288
ScaI AGT'ACT
Cuts at: 0 1029 1440
Size: 1029 411
SfcI C'TryA_G
Cuts at: 0 485 1440
Size: 485 955
SmlI C'TyrA_G
Cuts at: 0 1079 1165 1440
Size: 1079 86 275
Fragments arranged by size:
1079 275 86
StyI C'CwwG_G
Cuts at: 0 291 342 529 1440
Size: 291 51 187 911
Fragments arranged by size:
911 291 187 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1112 1440
Size: 1112 328
TatI w'GTAC_w
Cuts at: 0 1027 1440
Size: 1027 413
XbaI T'CTAG_A
Cuts at: 0 520 1440
Size: 520 920
ZraI GAC'GTC
Cuts at: 0 196 1440
Size: 196 1244
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI ApoI BaeI
BanII BbsI BbvCI BcgI BciVI BglI BmrI BpmI
Bpu10I BpuEI BsaHI BsaXI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BtgI BtsI DraI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI Hin4I HincII
MmeI MslI NcoI NspI PciI PpuMI PsiI PstI
SacI SalI ScaI SfcI SmlI StyI TaqII TatI
XbaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BseRI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoRV FalI FseI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI
XmaI XmnI