Restriction map for
pF1KB8595
(Linear) (Six-base) MAPSORT of: KIBB8595.seq Check: 2138 from: 1 to: 6606
KIBB8595.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1890 6606
Size: 1890 4716
AatII G_ACGT'C
Cuts at: 0 1659 4698 5286 6606
Size: 1659 3039 588 1320
Fragments arranged by size:
3039 1659 1320 588
AclI AA'CG_TT
Cuts at: 0 3300 6533 6606
Size: 3300 3233 73
Fragments arranged by size:
3300 3233 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 775 842 1250 1632 3074 3529 3898
Size: 737 38 67 408 382 1442 455 369
Cuts at: 3898 3954 4763 5546 6606
Size: 56 809 783 1060
Fragments arranged by size:
1442 1060 809 783 737 455 408 382
369 67 56 38
AflIII A'CryG_T
Cuts at: 0 1983 6606
Size: 1983 4623
AhdI GACnn_n'nnGTC
Cuts at: 0 3511 6606
Size: 3511 3095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1196 6606
Size: 1196 5410
AlwNI CAG_nnn'CTG
Cuts at: 0 1507 2670 3235 3817 4310 6606
Size: 1507 1163 565 582 493 2296
Fragments arranged by size:
2296 1507 1163 582 565 493
ApaI G_GGCC'C
Cuts at: 0 5953 6606
Size: 5953 653
ApoI r'AATT_y
Cuts at: 0 154 495 693 935 1547 1588 1721 2373
Size: 154 341 198 242 612 41 133 652
Cuts at: 2373 2525 2578 3058 4419 5143 6081 6606
Size: 152 53 480 1361 724 938 525
Fragments arranged by size:
1361 938 724 652 612 525 480 341
242 198 154 152 133 53 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2278 2311 6606
Size: 2278 33 4295
Fragments arranged by size:
4295 2278 33
BanII G_rGCy'C
Cuts at: 0 5953 6606
Size: 5953 653
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 4089 5614 6606
Size: 209 3880 1525 992
Fragments arranged by size:
3880 1525 992 209
BbvCI CC'TCA_GC
Cuts at: 0 3836 6606
Size: 3836 2770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1531 1565 6606
Size: 1531 34 5041
Fragments arranged by size:
5041 1531 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1112 6606
Size: 1112 5494
BclI T'GATC_A
Cuts at: 0 897 3063 3627 3687 6606
Size: 897 2166 564 60 2919
Fragments arranged by size:
2919 2166 897 564 60
BfrBI ATG'CAT
Cuts at: 0 519 4854 5248 6606
Size: 519 4335 394 1358
Fragments arranged by size:
4335 1358 519 394
BglI GCCn_nnn'nGGC
Cuts at: 0 4735 6606
Size: 4735 1871
BglII A'GATC_T
Cuts at: 0 735 6606
Size: 735 5871
Bme1580I G_kGCm'C
Cuts at: 0 1497 5953 6606
Size: 1497 4456 653
Fragments arranged by size:
4456 1497 653
BmgBI CAC'GTC
Cuts at: 0 1024 5787 6606
Size: 1024 4763 819
Fragments arranged by size:
4763 1024 819
BmrI ACTGGGnnnn_n'
Cuts at: 0 4549 5158 6606
Size: 4549 609 1448
Fragments arranged by size:
4549 1448 609
BmtI G_CTAG'C
Cuts at: 0 3374 6606
Size: 3374 3232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3613 3905 6606
Size: 3613 292 2701
Fragments arranged by size:
3613 2701 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 595 835 1082 3161 4643 5035 6606
Size: 193 402 240 247 2079 1482 392 1571
Fragments arranged by size:
2079 1571 1482 402 392 247 240 193
Bpu10I CC'TnA_GC
Cuts at: 0 3836 6606
Size: 3836 2770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 4807 6606
Size: 619 4188 1799
Fragments arranged by size:
4188 1799 619
BsaI GGTCTCn'nnnn_
Cuts at: 0 2980 4484 6579 6606
Size: 2980 1504 2095 27
Fragments arranged by size:
2980 2095 1504 27
BsaAI yAC'GTr
Cuts at: 0 4156 6606
Size: 4156 2450
BsaBI GATnn'nnATC
Cuts at: 0 5848 6606
Size: 5848 758
BsaHI Gr'CG_yC
Cuts at: 0 1656 4695 5283 6606
Size: 1656 3039 588 1323
Fragments arranged by size:
3039 1656 1323 588
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3202 3232 6606
Size: 3202 30 3374
Fragments arranged by size:
3374 3202 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 838 2923 3109 3215 3881 6302 6606
Size: 838 2085 186 106 666 2421 304
Fragments arranged by size:
2421 2085 838 666 304 186 106
BseYI C'CCAG_C
Cuts at: 0 813 5068 5299 6606
Size: 813 4255 231 1307
Fragments arranged by size:
4255 1307 813 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 6323 6539 6606
Size: 469 5854 216 67
Fragments arranged by size:
5854 469 216 67
BsiHKAI G_wGCw'C
Cuts at: 0 3276 4047 5192 6606
Size: 3276 771 1145 1414
Fragments arranged by size:
3276 1414 1145 771
BsmI GAATG_Cn'
Cuts at: 0 1149 2360 3258 4856 5521 6606
Size: 1149 1211 898 1598 665 1085
Fragments arranged by size:
1598 1211 1149 1085 898 665
BsmBI CGTCTCn'nnnn_
Cuts at: 0 19 6606
Size: 19 6587
Bsp1286I G_dGCh'C
Cuts at: 0 1497 3276 4047 5192 5953 6606
Size: 1497 1779 771 1145 761 653
Fragments arranged by size:
1779 1497 1145 771 761 653
BspHI T'CATG_A
Cuts at: 0 108 293 5115 5291 6482 6606
Size: 108 185 4822 176 1191 124
Fragments arranged by size:
4822 1191 185 176 124 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1890 6606
Size: 1890 4716
BsrDI GCAATG_nn'
Cuts at: 0 1520 1532 4250 4796 5783 6606
Size: 1520 12 2718 546 987 823
Fragments arranged by size:
2718 1520 987 823 546 12
BsrGI T'GTAC_A
Cuts at: 0 2163 2422 3962 3987 6606
Size: 2163 259 1540 25 2619
Fragments arranged by size:
2619 2163 1540 259 25
BssSI C'ACGA_G
Cuts at: 0 6132 6606
Size: 6132 474
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1255 2704 3244 3253 5215 6606
Size: 1255 1449 540 9 1962 1391
Fragments arranged by size:
1962 1449 1391 1255 540 9
BstBI TT'CG_AA
Cuts at: 0 6408 6606
Size: 6408 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1760 4289 4372 4760 6007 6606
Size: 1760 2529 83 388 1247 599
Fragments arranged by size:
2529 1760 1247 599 388 83
BstYI r'GATC_y
Cuts at: 0 735 3675 3909 4361 4749 6606
Size: 735 2940 234 452 388 1857
Fragments arranged by size:
2940 1857 735 452 388 234
Bsu36I CC'TnA_GG
Cuts at: 0 1106 6606
Size: 1106 5500
BtgI C'CryG_G
Cuts at: 0 6035 6606
Size: 6035 571
BtsI GCAGTG_nn'
Cuts at: 0 380 2744 3818 4765 6606
Size: 380 2364 1074 947 1841
Fragments arranged by size:
2364 1841 1074 947 380
DraI TTT'AAA
Cuts at: 0 1413 6606
Size: 1413 5193
DraIII CAC_nnn'GTG
Cuts at: 0 2151 6606
Size: 2151 4455
DrdI GACnn_nn'nnGTC
Cuts at: 0 4270 6606
Size: 4270 2336
EaeI y'GGCC_r
Cuts at: 0 2019 2226 5445 6606
Size: 2019 207 3219 1161
Fragments arranged by size:
3219 2019 1161 207
EarI CTCTTCn'nnn_
Cuts at: 0 172 295 920 1417 3077 5880 5885 6606
Size: 172 123 625 497 1660 2803 5 721
Fragments arranged by size:
2803 1660 721 625 497 172 123 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 595 737 775 835 842 1082 1250
Size: 193 402 142 38 60 7 240 168
Cuts at: 1250 1632 3074 3161 3529 3898 3954 4643 4763
Size: 382 1442 87 368 369 56 689 120
Cuts at: 4763 5035 5546 6606
Size: 272 511 1060
Fragments arranged by size:
1442 1060 689 511 402 382 369 368
272 240 193 168 142 120 87 60
56 38 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5683 6606
Size: 5683 923
EcoO109I rG'GnC_Cy
Cuts at: 0 817 4387 5949 6606
Size: 817 3570 1562 657
Fragments arranged by size:
3570 1562 817 657
EcoRV GAT'ATC
Cuts at: 0 3030 6606
Size: 3030 3576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2774 4213 5148 5520 5551 6565 6606
Size: 2774 1439 935 372 31 1014 41
Fragments arranged by size:
2774 1439 1014 935 372 41 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2535 2567 2769 2801 3202 3234 3497 3529
Size: 2535 32 202 32 401 32 263 32
Cuts at: 3529 4231 4263 5273 5305 5841 5873 6606
Size: 702 32 1010 32 536 32 733
Fragments arranged by size:
2535 1010 733 702 536 401 263 202
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2517 4594 6564 6606
Size: 2517 2077 1970 42
Fragments arranged by size:
2517 2077 1970 42
HindIII A'AGCT_T
Cuts at: 0 1306 3433 6606
Size: 1306 2127 3173
Fragments arranged by size:
3173 2127 1306
MfeI C'AATT_G
Cuts at: 0 266 866 924 2654 6606
Size: 266 600 58 1730 3952
Fragments arranged by size:
3952 1730 600 266 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 2850 4440 4563 4841 5304 5947 6606
Size: 222 2628 1590 123 278 463 643 659
Fragments arranged by size:
2628 1590 659 643 463 278 222 123
MscI TGG'CCA
Cuts at: 0 2228 6606
Size: 2228 4378
MslI CAynn'nnrTG
Cuts at: 0 1214 1525 1678 2264 5204 6005 6606
Size: 1214 311 153 586 2940 801 601
Fragments arranged by size:
2940 1214 801 601 586 311 153
MspA1I CmG'CkG
Cuts at: 0 1404 1630 4075 6606
Size: 1404 226 2445 2531
Fragments arranged by size:
2531 2445 1404 226
NcoI C'CATG_G
Cuts at: 0 6035 6606
Size: 6035 571
NdeI CA'TA_TG
Cuts at: 0 2796 3240 3951 5597 6606
Size: 2796 444 711 1646 1009
Fragments arranged by size:
2796 1646 1009 711 444
NheI G'CTAG_C
Cuts at: 0 3370 6606
Size: 3370 3236
NsiI A_TGCA'T
Cuts at: 0 521 4856 5250 6606
Size: 521 4335 394 1356
Fragments arranged by size:
4335 1356 521 394
NspI r_CATG'y
Cuts at: 0 226 519 1485 1704 1987 2170 5959 6058
Size: 226 293 966 219 283 183 3789 99
Cuts at: 6058 6226 6606
Size: 168 380
Fragments arranged by size:
3789 966 380 293 283 226 219 183
168 99
PciI A'CATG_T
Cuts at: 0 1983 6606
Size: 1983 4623
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 1999 2154 3756 5058 6606
Size: 553 1446 155 1602 1302 1548
Fragments arranged by size:
1602 1548 1446 1302 553 155
PfoI T'CCnGG_A
Cuts at: 0 611 3138 6606
Size: 611 2527 3468
Fragments arranged by size:
3468 2527 611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4376 4408 6606
Size: 4376 32 2198
Fragments arranged by size:
4376 2198 32
PpuMI rG'GwC_Cy
Cuts at: 0 817 6606
Size: 817 5789
PsiI TTA'TAA
Cuts at: 0 65 587 2599 5138 6606
Size: 65 522 2012 2539 1468
Fragments arranged by size:
2539 2012 1468 522 65
PspOMI G'GGCC_C
Cuts at: 0 5949 6606
Size: 5949 657
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4541 4573 5096 5128 6606
Size: 4541 32 523 32 1478
Fragments arranged by size:
4541 1478 523 32 32
PstI C_TGCA'G
Cuts at: 0 387 6189 6606
Size: 387 5802 417
Fragments arranged by size:
5802 417 387
PvuII CAG'CTG
Cuts at: 0 1404 1630 4075 6606
Size: 1404 226 2445 2531
Fragments arranged by size:
2531 2445 1404 226
SapI GCTCTTCn'nnn_
Cuts at: 0 920 6606
Size: 920 5686
ScaI AGT'ACT
Cuts at: 0 5523 6606
Size: 5523 1083
SexAI A'CCwGG_T
Cuts at: 0 2147 6606
Size: 2147 4459
SfcI C'TryA_G
Cuts at: 0 383 1332 1499 3318 5683 6185 6606
Size: 383 949 167 1819 2365 502 421
Fragments arranged by size:
2365 1819 949 502 421 383 167
SmlI C'TyrA_G
Cuts at: 0 634 4822 6606
Size: 634 4188 1784
Fragments arranged by size:
4188 1784 634
SphI G_CATG'C
Cuts at: 0 6226 6606
Size: 6226 380
SspI AAT'ATT
Cuts at: 0 3501 4001 4512 6606
Size: 3501 500 511 2094
Fragments arranged by size:
3501 2094 511 500
StyI C'CwwG_G
Cuts at: 0 1622 2253 2520 3655 4256 5091 6035 6498
Size: 1622 631 267 1135 601 835 944 463
Cuts at: 6498 6569 6606
Size: 71 37
Fragments arranged by size:
1622 1135 944 835 631 601 463 267
71 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3820 4895 5305 6606
Size: 3820 1075 410 1301
Fragments arranged by size:
3820 1301 1075 410
TatI w'GTAC_w
Cuts at: 0 745 2163 2268 2422 3962 3987 5521 6606
Size: 745 1418 105 154 1540 25 1534 1085
Fragments arranged by size:
1540 1534 1418 1085 745 154 105 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2052 2084 2135 2167 3392 3424 6606
Size: 2052 32 51 32 1225 32 3182
Fragments arranged by size:
3182 2052 1225 51 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1659 4395 6606
Size: 1659 2736 2211
Fragments arranged by size:
2736 2211 1659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5745 6606
Size: 5745 861
XmnI GAAnn'nnTTC
Cuts at: 0 411 4653 6606
Size: 411 4242 1953
Fragments arranged by size:
4242 1953 411
ZraI GAC'GTC
Cuts at: 0 1657 4696 5284 6606
Size: 1657 3039 588 1322
Fragments arranged by size:
3039 1657 1322 588
Enzymes that do cut:
AarI AatII AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI BaeI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PciI PflMI PfoI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuII SapI ScaI SexAI
SfcI SmlI SphI SspI StyI TaqII TatI TstI
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AflII AgeI AleI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BlpI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII
BstEII BstZ17I BtgZI ClaI CspCI EagI EciI EcoICRI
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI PacI PasI
PmeI PmlI PshAI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI