(Linear) (Six-base) MAPSORT of: KIBB8597.seq  Check: 164  from: 1  to: 762



KIBB8597.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AflIII A'CryG_T

Cuts at:      0     608     762 
   Size:        608     154
 
AhdI GACnn_n'nnGTC

Cuts at:      0      75     762 
   Size:         75     687
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     265     297     762 
   Size:        265      32     465

  Fragments arranged by size: 

                 465     265      32
 
ApaI G_GGCC'C

Cuts at:      0     719     762 
   Size:        719      43
 
ApoI r'AATT_y

Cuts at:      0      55     762 
   Size:         55     707
 
BamHI G'GATC_C

Cuts at:      0     340     762 
   Size:        340     422
 
BanII G_rGCy'C

Cuts at:      0     440     520     638     719     762 
   Size:        440      80     118      81      43

  Fragments arranged by size: 

                 440     118      81      80      43
 
BclI T'GATC_A

Cuts at:      0      89     762 
   Size:         89     673
 
BlpI GC'TnA_GC

Cuts at:      0     427     762 
   Size:        427     335
 
Bme1580I G_kGCm'C

Cuts at:      0     719     762 
   Size:        719      43
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     687     762 
   Size:        687      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     552     762 
   Size:        504      48     210

  Fragments arranged by size: 

                 504     210      48
 
Bpu10I CC'TnA_GC

Cuts at:      0     458     762 
   Size:        458     304
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577     762 
   Size:        547      30     185

  Fragments arranged by size: 

                 547     185      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     555     676     682     762 
   Size:        555     121       6      80

  Fragments arranged by size: 

                 555     121      80       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     638     762 
   Size:        308     330     124

  Fragments arranged by size: 

                 330     308     124
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     440     520     638     719     762 
   Size:        308     132      80     118      81      43

  Fragments arranged by size: 

                 308     132     118      81      80      43
 
BssSI C'ACGA_G

Cuts at:      0     510     762 
   Size:        510     252
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     235     762 
   Size:        235     527
 
BstYI r'GATC_y

Cuts at:      0     340     762 
   Size:        340     422
 
DraI TTT'AAA

Cuts at:      0      60     762 
   Size:         60     702
 
DraIII CAC_nnn'GTG

Cuts at:      0     512     762 
   Size:        512     250
 
EaeI y'GGCC_r

Cuts at:      0     595     762 
   Size:        595     167
 
EcoICRI GAG'CTC

Cuts at:      0     636     762 
   Size:        636     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     552     762 
   Size:        504      48     210

  Fragments arranged by size: 

                 504     210      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     716     762 
   Size:        716      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     547     579     762 
   Size:         61      32     454      32     183

  Fragments arranged by size: 

                 454     183      61      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     762 
   Size:        458     304
 
MscI TGG'CCA

Cuts at:      0     597     762 
   Size:        597     165
 
MspA1I CmG'CkG

Cuts at:      0     524     762 
   Size:        524     238
 
NspI r_CATG'y

Cuts at:      0     612     762 
   Size:        612     150
 
PasI CC'CwG_GG

Cuts at:      0     235     762 
   Size:        235     527
 
PciI A'CATG_T

Cuts at:      0     608     762 
   Size:        608     154
 
PspOMI G'GGCC_C

Cuts at:      0     715     762 
   Size:        715      47
 
PstI C_TGCA'G

Cuts at:      0     704     762 
   Size:        704      58
 
SacI G_AGCT'C

Cuts at:      0     638     762 
   Size:        638     124
 
SfcI C'TryA_G

Cuts at:      0     700     762 
   Size:        700      62
 
StyI C'CwwG_G

Cuts at:      0     546     762 
   Size:        546     216
 
Tth111I GACn'n_nGTC

Cuts at:      0     654     762 
   Size:        654     108
 

 Enzymes that do cut:

   AflIII     AhdI     AloI     ApaI     ApoI    BamHI    BanII     BclI
     BlpI Bme1580I     BplI     BpmI   Bpu10I    BsaXI    BseRI  BsiHKAI
 Bsp1286I    BssSI    BstXI    BstYI     DraI   DraIII     EaeI  EcoICRI
  Eco57MI EcoO109I    Hin4I     MmeI     MscI   MspA1I     NspI     PasI
     PciI   PspOMI     PstI     SacI     SfcI     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI