Restriction map for
pF1KB8598
(Linear) (Six-base) MAPSORT of: KIBB8598.seq Check: 2442 from: 1 to: 2328
KIBB8598.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 2328
Size: 78 2250
AccI GT'mk_AC
Cuts at: 0 1810 2328
Size: 1810 518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1495 1819 2328
Size: 55 1440 324 509
Fragments arranged by size:
1440 509 324 55
AflII C'TTAA_G
Cuts at: 0 274 310 2328
Size: 274 36 2018
Fragments arranged by size:
2018 274 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1630 2328
Size: 1630 698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 2328
Size: 182 32 2114
Fragments arranged by size:
2114 182 32
AlwNI CAG_nnn'CTG
Cuts at: 0 772 1872 2328
Size: 772 1100 456
Fragments arranged by size:
1100 772 456
ApaI G_GGCC'C
Cuts at: 0 667 2328
Size: 667 1661
ApoI r'AATT_y
Cuts at: 0 280 349 404 748 902 916 924 2328
Size: 280 69 55 344 154 14 8 1404
Fragments arranged by size:
1404 344 280 154 69 55 14 8
AvaI C'yCGr_G
Cuts at: 0 2231 2328
Size: 2231 97
AvrII C'CTAG_G
Cuts at: 0 996 2328
Size: 996 1332
BanII G_rGCy'C
Cuts at: 0 134 243 667 1624 2328
Size: 134 109 424 957 704
Fragments arranged by size:
957 704 424 134 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 1334 1347 1376 2120 2328
Size: 1334 13 29 744 208
Fragments arranged by size:
1334 744 208 29 13
BclI T'GATC_A
Cuts at: 0 2243 2328
Size: 2243 85
BfrBI ATG'CAT
Cuts at: 0 652 2255 2328
Size: 652 1603 73
Fragments arranged by size:
1603 652 73
BglI GCCn_nnn'nGGC
Cuts at: 0 1724 2328
Size: 1724 604
BglII A'GATC_T
Cuts at: 0 56 1545 2328
Size: 56 1489 783
Fragments arranged by size:
1489 783 56
BlpI GC'TnA_GC
Cuts at: 0 1618 2328
Size: 1618 710
Bme1580I G_kGCm'C
Cuts at: 0 667 2328
Size: 667 1661
BmgBI CAC'GTC
Cuts at: 0 1739 2328
Size: 1739 589
BmrI ACTGGGnnnn_n'
Cuts at: 0 760 2328
Size: 760 1568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1518 2328
Size: 1518 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 254 2202 2328
Size: 215 39 1948 126
Fragments arranged by size:
1948 215 126 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 2328
Size: 826 1502
BsaAI yAC'GTr
Cuts at: 0 624 2328
Size: 624 1704
BsaBI GATnn'nnATC
Cuts at: 0 1825 2328
Size: 1825 503
BsaHI Gr'CG_yC
Cuts at: 0 1807 2328
Size: 1807 521
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 492 522 1264 1294 1754 1784
Size: 182 30 280 30 742 30 460 30
Cuts at: 1784 2328
Size: 544
Fragments arranged by size:
742 544 460 280 182 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 203 251 1327 2328
Size: 203 48 1076 1001
Fragments arranged by size:
1076 1001 203 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1885 2328
Size: 1885 443
BsiHKAI G_wGCw'C
Cuts at: 0 134 243 1624 2328
Size: 134 109 1381 704
Fragments arranged by size:
1381 704 134 109
BsmI GAATG_Cn'
Cuts at: 0 1150 1607 2328
Size: 1150 457 721
Fragments arranged by size:
1150 721 457
Bsp1286I G_dGCh'C
Cuts at: 0 134 243 667 1624 2328
Size: 134 109 424 957 704
Fragments arranged by size:
957 704 424 134 109
BspHI T'CATG_A
Cuts at: 0 837 2153 2328
Size: 837 1316 175
Fragments arranged by size:
1316 837 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 2328
Size: 78 2250
BsrFI r'CCGG_y
Cuts at: 0 76 2328
Size: 76 2252
BsrGI T'GTAC_A
Cuts at: 0 92 612 1098 1651 2328
Size: 92 520 486 553 677
Fragments arranged by size:
677 553 520 486 92
BssSI C'ACGA_G
Cuts at: 0 185 2328
Size: 185 2143
BstEII G'GTnAC_C
Cuts at: 0 1221 2328
Size: 1221 1107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 2328
Size: 126 2202
BstYI r'GATC_y
Cuts at: 0 56 1545 2328
Size: 56 1489 783
Fragments arranged by size:
1489 783 56
BtgI C'CryG_G
Cuts at: 0 27 2328
Size: 27 2301
ClaI AT'CG_AT
Cuts at: 0 378 2328
Size: 378 1950
DraI TTT'AAA
Cuts at: 0 551 1079 2328
Size: 551 528 1249
Fragments arranged by size:
1249 551 528
EaeI y'GGCC_r
Cuts at: 0 110 1725 2138 2328
Size: 110 1615 413 190
Fragments arranged by size:
1615 413 190 110
EarI CTCTTCn'nnn_
Cuts at: 0 813 2328
Size: 813 1515
EcoICRI GAG'CTC
Cuts at: 0 132 241 1622 2328
Size: 132 109 1381 706
Fragments arranged by size:
1381 706 132 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 215 254 1495 1819 2202 2328
Size: 55 160 39 1241 324 383 126
Fragments arranged by size:
1241 383 324 160 126 55 39
EcoO109I rG'GnC_Cy
Cuts at: 0 663 664 1628 2199 2328
Size: 663 1 964 571 129
Fragments arranged by size:
964 663 571 129 1
EcoRI G'AATT_C
Cuts at: 0 748 2328
Size: 748 1580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 2328
Size: 312 2016
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 492 524 1530 1562 2328
Size: 65 32 395 32 1006 32 766
Fragments arranged by size:
1006 766 395 65 32 32 32
HincII GTy'rAC
Cuts at: 0 8 674 1811 2046 2328
Size: 8 666 1137 235 282
Fragments arranged by size:
1137 666 282 235 8
HindIII A'AGCT_T
Cuts at: 0 1481 2328
Size: 1481 847
HpaI GTT'AAC
Cuts at: 0 674 2046 2328
Size: 674 1372 282
Fragments arranged by size:
1372 674 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1655 2328
Size: 998 657 673
Fragments arranged by size:
998 673 657
MscI TGG'CCA
Cuts at: 0 112 2328
Size: 112 2216
MslI CAynn'nnrTG
Cuts at: 0 1534 1612 2053 2158 2328
Size: 1534 78 441 105 170
Fragments arranged by size:
1534 441 170 105 78
MspA1I CmG'CkG
Cuts at: 0 1872 2211 2328
Size: 1872 339 117
Fragments arranged by size:
1872 339 117
NaeI GCC'GGC
Cuts at: 0 78 2328
Size: 78 2250
NgoMIV G'CCGG_C
Cuts at: 0 76 2328
Size: 76 2252
NsiI A_TGCA'T
Cuts at: 0 654 2257 2328
Size: 654 1603 71
Fragments arranged by size:
1603 654 71
NspI r_CATG'y
Cuts at: 0 966 1521 2328
Size: 966 555 807
Fragments arranged by size:
966 807 555
PfoI T'CCnGG_A
Cuts at: 0 986 2328
Size: 986 1342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 866 898 2328
Size: 182 32 652 32 1430
Fragments arranged by size:
1430 652 182 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1628 2199 2328
Size: 1628 571 129
Fragments arranged by size:
1628 571 129
PsiI TTA'TAA
Cuts at: 0 149 2024 2328
Size: 149 1875 304
Fragments arranged by size:
1875 304 149
PspOMI G'GGCC_C
Cuts at: 0 663 2328
Size: 663 1665
PspXI vC'TCGA_Gb
Cuts at: 0 2231 2328
Size: 2231 97
PvuII CAG'CTG
Cuts at: 0 1872 2211 2328
Size: 1872 339 117
Fragments arranged by size:
1872 339 117
SacI G_AGCT'C
Cuts at: 0 134 243 1624 2328
Size: 134 109 1381 704
Fragments arranged by size:
1381 704 134 109
SalI G'TCGA_C
Cuts at: 0 1809 2328
Size: 1809 519
ScaI AGT'ACT
Cuts at: 0 744 2328
Size: 744 1584
SfcI C'TryA_G
Cuts at: 0 2313 2328
Size: 2313 15
SmlI C'TyrA_G
Cuts at: 0 274 310 805 2231 2328
Size: 274 36 495 1426 97
Fragments arranged by size:
1426 495 274 97 36
SpeI A'CTAG_T
Cuts at: 0 160 2328
Size: 160 2168
SspI AAT'ATT
Cuts at: 0 2281 2328
Size: 2281 47
StyI C'CwwG_G
Cuts at: 0 996 2328
Size: 996 1332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1891 2328
Size: 1891 437
TatI w'GTAC_w
Cuts at: 0 92 157 342 612 742 1098 1361 1496
Size: 92 65 185 270 130 356 263 135
Cuts at: 1496 1651 1838 2328
Size: 155 187 490
Fragments arranged by size:
490 356 270 263 187 185 155 135
130 92 65
XbaI T'CTAG_A
Cuts at: 0 979 2328
Size: 979 1349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2188 2328
Size: 2188 140
XhoI C'TCGA_G
Cuts at: 0 2231 2328
Size: 2231 97
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AloI AlwNI ApaI
ApoI AvaI AvrII BanII BbsI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstEII BstXI
BstYI BtgI ClaI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PfoI
PpiI PpuMI PsiI PspOMI PspXI PvuII SacI SalI
ScaI SfcI SmlI SpeI SspI StyI TaqII TatI
XbaI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
ApaLI AscI AseI AsiSI BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BmtI Bpu10I BsaI BsaWI BseYI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI CspCI DraIII DrdI
EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PshAI PsrI PstI PvuI RsrII SacII SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TstI Tth111I XmaI XmnI
ZraI