(Linear) (Six-base) MAPSORT of: KIBB8601.seq  Check: 1789  from: 1  to: 504



KIBB8601.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      36     504 
   Size:         36     468
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     504 
   Size:        253     251
 
ApoI r'AATT_y

Cuts at:      0     217     504 
   Size:        217     287
 
BclI T'GATC_A

Cuts at:      0     363     504 
   Size:        363     141
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     504 
   Size:        136     368
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     373     504 
   Size:        169     204     131

  Fragments arranged by size: 

                 204     169     131
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     471     504 
   Size:        471      33
 
BseYI C'CCAG_C

Cuts at:      0     369     504 
   Size:        369     135
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     504 
   Size:        111     393
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      36     504 
   Size:         36     468
 
BsrDI GCAATG_nn'

Cuts at:      0     144     504 
   Size:        144     360
 
BssSI C'ACGA_G

Cuts at:      0     490     504 
   Size:        490      14
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      28     134     379     504 
   Size:         28     106     245     125

  Fragments arranged by size: 

                 245     125     106      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     218     272     504 
   Size:        127      91      54     232

  Fragments arranged by size: 

                 232     127      91      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     504 
   Size:        253     251
 
EcoO109I rG'GnC_Cy

Cuts at:      0     251     504 
   Size:        251     253
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      69     504 
   Size:         69     435
 
FspI TGC'GCA

Cuts at:      0     287     504 
   Size:        287     217
 
HincII GTy'rAC

Cuts at:      0     501     504 
   Size:        501       3
 
MslI CAynn'nnrTG

Cuts at:      0     107     504 
   Size:        107     397
 
PfoI T'CCnGG_A

Cuts at:      0     311     504 
   Size:        311     193
 
SapI GCTCTTCn'nnn_

Cuts at:      0     218     504 
   Size:        218     286
 
ScaI AGT'ACT

Cuts at:      0     282     504 
   Size:        282     222
 
SmlI C'TyrA_G

Cuts at:      0     184     352     504 
   Size:        184     168     152

  Fragments arranged by size: 

                 184     168     152
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     383     504 
   Size:        383     121
 
TatI w'GTAC_w

Cuts at:      0     280     504 
   Size:        280     224
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     403     504 
   Size:        403     101
 
XmnI GAAnn'nnTTC

Cuts at:      0      39     504 
   Size:         39     465
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BclI     BplI    BpuEI    BseRI    BseYI
     BsgI    BspMI    BsrDI    BssSI   BstAPI     EarI  Eco57MI EcoO109I
     FalI     FspI   HincII     MslI     PfoI     SapI     ScaI     SmlI
    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI