Restriction map for
pF1KB8602
(Linear) (Six-base) MAPSORT of: KIBB8602.seq Check: 3145 from: 1 to: 636
KIBB8602.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 246 358 636
Size: 220 26 112 278
Fragments arranged by size:
278 220 112 26
AleI CACnn'nnGTG
Cuts at: 0 172 636
Size: 172 464
AlwNI CAG_nnn'CTG
Cuts at: 0 248 336 636
Size: 248 88 300
Fragments arranged by size:
300 248 88
ApaI G_GGCC'C
Cuts at: 0 608 636
Size: 608 28
BanI G'GyrC_C
Cuts at: 0 187 268 636
Size: 187 81 368
Fragments arranged by size:
368 187 81
BanII G_rGCy'C
Cuts at: 0 608 636
Size: 608 28
BbvCI CC'TCA_GC
Cuts at: 0 406 636
Size: 406 230
BciVI GTATCCnnnnn_n'
Cuts at: 0 148 636
Size: 148 488
BglI GCCn_nnn'nGGC
Cuts at: 0 612 636
Size: 612 24
BglII A'GATC_T
Cuts at: 0 209 636
Size: 209 427
BlpI GC'TnA_GC
Cuts at: 0 326 636
Size: 326 310
Bme1580I G_kGCm'C
Cuts at: 0 608 636
Size: 608 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 636
Size: 367 269
Bpu10I CC'TnA_GC
Cuts at: 0 406 636
Size: 406 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 304 636
Size: 226 78 332
Fragments arranged by size:
332 226 78
BsaAI yAC'GTr
Cuts at: 0 117 636
Size: 117 519
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 367 571 636
Size: 364 3 204 65
Fragments arranged by size:
364 204 65 3
BseYI C'CCAG_C
Cuts at: 0 23 40 636
Size: 23 17 596
Fragments arranged by size:
596 23 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 636
Size: 343 293
Bsp1286I G_dGCh'C
Cuts at: 0 608 636
Size: 608 28
BsrBI CCG'CTC
Cuts at: 0 417 636
Size: 417 219
BstEII G'GTnAC_C
Cuts at: 0 423 636
Size: 423 213
BstYI r'GATC_y
Cuts at: 0 209 636
Size: 209 427
BtgI C'CryG_G
Cuts at: 0 86 636
Size: 86 550
BtsI GCAGTG_nn'
Cuts at: 0 337 636
Size: 337 299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 158 193 636
Size: 158 35 443
Fragments arranged by size:
443 158 35
DraIII CAC_nnn'GTG
Cuts at: 0 504 636
Size: 504 132
EaeI y'GGCC_r
Cuts at: 0 225 485 636
Size: 225 260 151
Fragments arranged by size:
260 225 151
EarI CTCTTCn'nnn_
Cuts at: 0 113 636
Size: 113 523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 246 358 367 636
Size: 220 26 112 9 269
Fragments arranged by size:
269 220 112 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 409 636
Size: 349 60 227
Fragments arranged by size:
349 227 60
EcoO109I rG'GnC_Cy
Cuts at: 0 17 342 604 636
Size: 17 325 262 32
Fragments arranged by size:
325 262 32 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 144 330 636
Size: 117 27 186 306
Fragments arranged by size:
306 186 117 27
HindIII A'AGCT_T
Cuts at: 0 202 636
Size: 202 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 636
Size: 488 148
MscI TGG'CCA
Cuts at: 0 227 636
Size: 227 409
MslI CAynn'nnrTG
Cuts at: 0 172 636
Size: 172 464
MspA1I CmG'CkG
Cuts at: 0 223 586 636
Size: 223 363 50
Fragments arranged by size:
363 223 50
PasI CC'CwG_GG
Cuts at: 0 29 636
Size: 29 607
PfoI T'CCnGG_A
Cuts at: 0 431 636
Size: 431 205
PpuMI rG'GwC_Cy
Cuts at: 0 17 342 636
Size: 17 325 294
Fragments arranged by size:
325 294 17
PspOMI G'GGCC_C
Cuts at: 0 604 636
Size: 604 32
PstI C_TGCA'G
Cuts at: 0 545 636
Size: 545 91
PvuII CAG'CTG
Cuts at: 0 223 586 636
Size: 223 363 50
Fragments arranged by size:
363 223 50
SapI GCTCTTCn'nnn_
Cuts at: 0 113 636
Size: 113 523
SexAI A'CCwGG_T
Cuts at: 0 500 636
Size: 500 136
SfcI C'TryA_G
Cuts at: 0 541 636
Size: 541 95
SmlI C'TyrA_G
Cuts at: 0 205 319 636
Size: 205 114 317
Fragments arranged by size:
317 205 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 377 636
Size: 175 202 259
Fragments arranged by size:
259 202 175
XmnI GAAnn'nnTTC
Cuts at: 0 127 636
Size: 127 509
Enzymes that do cut:
AcuI AleI AlwNI ApaI BanI BanII BbvCI BciVI
BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI
BseRI BseYI BsgI Bsp1286I BsrBI BstEII BstYI BtgI
BtsI CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
FalI HindIII MmeI MscI MslI MspA1I PasI PfoI
PpuMI PspOMI PstI PvuII SapI SexAI SfcI SmlI
TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BclI BfrBI BmgBI BmrI BmtI BplI BsaI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI