Restriction map for
pF1KB8603
(Linear) (Six-base) MAPSORT of: KIBB8603.seq Check: 6032 from: 1 to: 1236
KIBB8603.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
Acc65I G'GTAC_C
Cuts at: 0 110 1236
Size: 110 1126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1236
Size: 793 443
AfeI AGC'GCT
Cuts at: 0 1114 1236
Size: 1114 122
AflII C'TTAA_G
Cuts at: 0 289 1236
Size: 289 947
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1236
Size: 1001 32 203
Fragments arranged by size:
1001 203 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1189 1236
Size: 1189 47
ApoI r'AATT_y
Cuts at: 0 4 196 1236
Size: 4 192 1040
Fragments arranged by size:
1040 192 4
AvaI C'yCGr_G
Cuts at: 0 932 1236
Size: 932 304
BamHI G'GATC_C
Cuts at: 0 550 1236
Size: 550 686
BanI G'GyrC_C
Cuts at: 0 110 975 1236
Size: 110 865 261
Fragments arranged by size:
865 261 110
BanII G_rGCy'C
Cuts at: 0 926 1236
Size: 926 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 878 1236
Size: 878 358
BbvCI CC'TCA_GC
Cuts at: 0 714 1236
Size: 714 522
BfrBI ATG'CAT
Cuts at: 0 376 1236
Size: 376 860
BlpI GC'TnA_GC
Cuts at: 0 310 1236
Size: 310 926
Bme1580I G_kGCm'C
Cuts at: 0 980 1236
Size: 980 256
BmrI ACTGGGnnnn_n'
Cuts at: 0 955 1236
Size: 955 281
Bpu10I CC'TnA_GC
Cuts at: 0 714 1236
Size: 714 522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1236
Size: 148 1088
BsaWI w'CCGG_w
Cuts at: 0 671 1236
Size: 671 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1186 1216 1236
Size: 760 30 396 30 20
Fragments arranged by size:
760 396 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 872 1236
Size: 763 109 364
Fragments arranged by size:
763 364 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1236
Size: 212 1024
BsiHKAI G_wGCw'C
Cuts at: 0 943 1236
Size: 943 293
BsmI GAATG_Cn'
Cuts at: 0 65 852 1236
Size: 65 787 384
Fragments arranged by size:
787 384 65
Bsp1286I G_dGCh'C
Cuts at: 0 926 943 980 1236
Size: 926 17 37 256
Fragments arranged by size:
926 256 37 17
BspEI T'CCGG_A
Cuts at: 0 671 1236
Size: 671 565
BspHI T'CATG_A
Cuts at: 0 911 1175 1236
Size: 911 264 61
Fragments arranged by size:
911 264 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 1236
Size: 131 1105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 382 1236
Size: 382 854
BstYI r'GATC_y
Cuts at: 0 37 550 753 1236
Size: 37 513 203 483
Fragments arranged by size:
513 483 203 37
BtsI GCAGTG_nn'
Cuts at: 0 458 1236
Size: 458 778
EarI CTCTTCn'nnn_
Cuts at: 0 508 527 1236
Size: 508 19 709
Fragments arranged by size:
709 508 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1236
Size: 793 443
EcoRI G'AATT_C
Cuts at: 0 196 1236
Size: 196 1040
EcoRV GAT'ATC
Cuts at: 0 786 1236
Size: 786 450
HaeII r_GCGC'y
Cuts at: 0 1116 1236
Size: 1116 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 303 335 760 792 1087 1119 1236
Size: 303 32 425 32 295 32 117
Fragments arranged by size:
425 303 295 117 32 32 32
HincII GTy'rAC
Cuts at: 0 1062 1236
Size: 1062 174
KpnI G_GTAC'C
Cuts at: 0 114 1236
Size: 114 1122
MfeI C'AATT_G
Cuts at: 0 1164 1236
Size: 1164 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1035 1236
Size: 117 918 201
Fragments arranged by size:
918 201 117
MslI CAynn'nnrTG
Cuts at: 0 475 1236
Size: 475 761
NsiI A_TGCA'T
Cuts at: 0 378 1236
Size: 378 858
PsiI TTA'TAA
Cuts at: 0 799 1236
Size: 799 437
PstI C_TGCA'G
Cuts at: 0 261 1236
Size: 261 975
SapI GCTCTTCn'nnn_
Cuts at: 0 508 527 1236
Size: 508 19 709
Fragments arranged by size:
709 508 19
SexAI A'CCwGG_T
Cuts at: 0 1049 1236
Size: 1049 187
SfcI C'TryA_G
Cuts at: 0 257 559 789 1236
Size: 257 302 230 447
Fragments arranged by size:
447 302 257 230
SmlI C'TyrA_G
Cuts at: 0 127 289 932 1224 1236
Size: 127 162 643 292 12
Fragments arranged by size:
643 292 162 127 12
XhoI C'TCGA_G
Cuts at: 0 932 1236
Size: 932 304
XmnI GAAnn'nnTTC
Cuts at: 0 422 683 1236
Size: 422 261 553
Fragments arranged by size:
553 422 261
Enzymes that do cut:
Acc65I AcuI AfeI AflII AloI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BfrBI BlpI Bme1580I
BmrI Bpu10I BpuEI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BstAPI BstYI BtsI
EarI Eco57MI EcoRI EcoRV HaeII Hin4I HincII KpnI
MfeI MmeI MslI NsiI PsiI PstI SapI SexAI
SfcI SmlI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflIII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BglI BglII BmgBI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaHI BseYI BsiEI
BsiWI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I FalI FseI FspI FspAI HindIII HpaI
KasI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI