Restriction map for
pF1KB8607
(Linear) (Six-base) MAPSORT of: KIBB8607.seq Check: 2603 from: 1 to: 2058
KIBB8607.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 0 251 2058
Size: 251 1807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1478 2058
Size: 347 1131 580
Fragments arranged by size:
1131 580 347
AflIII A'CryG_T
Cuts at: 0 650 1103 1658 1763 2058
Size: 650 453 555 105 295
Fragments arranged by size:
650 555 453 295 105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1801 2058
Size: 1801 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 859 891 2058
Size: 859 32 1167
Fragments arranged by size:
1167 859 32
ApaI G_GGCC'C
Cuts at: 0 1205 2058
Size: 1205 853
ApoI r'AATT_y
Cuts at: 0 412 1972 2058
Size: 412 1560 86
Fragments arranged by size:
1560 412 86
AvaI C'yCGr_G
Cuts at: 0 794 1082 2058
Size: 794 288 976
Fragments arranged by size:
976 794 288
BanI G'GyrC_C
Cuts at: 0 49 2058
Size: 49 2009
BanII G_rGCy'C
Cuts at: 0 1205 2058
Size: 1205 853
BbsI GAAGACnn'nnnn_
Cuts at: 0 1820 2058
Size: 1820 238
BbvCI CC'TCA_GC
Cuts at: 0 224 2058
Size: 224 1834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 896 2058
Size: 862 34 1162
Fragments arranged by size:
1162 862 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 159 2058
Size: 159 1899
BglI GCCn_nnn'nGGC
Cuts at: 0 727 2058
Size: 727 1331
BglII A'GATC_T
Cuts at: 0 1038 1325 2058
Size: 1038 287 733
Fragments arranged by size:
1038 733 287
Bme1580I G_kGCm'C
Cuts at: 0 54 1205 1780 2058
Size: 54 1151 575 278
Fragments arranged by size:
1151 575 278 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 649 1652 2058
Size: 649 1003 406
Fragments arranged by size:
1003 649 406
BmtI G_CTAG'C
Cuts at: 0 1153 2058
Size: 1153 905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1538 2058
Size: 1136 402 520
Fragments arranged by size:
1136 520 402
Bpu10I CC'TnA_GC
Cuts at: 0 224 353 1134 2058
Size: 224 129 781 924
Fragments arranged by size:
924 781 224 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 1112 2058
Size: 1112 946
BsaAI yAC'GTr
Cuts at: 0 657 2058
Size: 657 1401
BsaHI Gr'CG_yC
Cuts at: 0 14 2058
Size: 14 2044
BsaWI w'CCGG_w
Cuts at: 0 1571 2058
Size: 1571 487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 859 889 926 956 1833 1863
Size: 265 30 564 30 37 30 877 30
Cuts at: 1863 2058
Size: 195
Fragments arranged by size:
877 564 265 195 37 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 593 1605 1739 1854 1946 2058
Size: 593 1012 134 115 92 112
Fragments arranged by size:
1012 593 134 115 112 92
BseYI C'CCAG_C
Cuts at: 0 67 289 2058
Size: 67 222 1769
Fragments arranged by size:
1769 222 67
BsiHKAI G_wGCw'C
Cuts at: 0 94 1878 2058
Size: 94 1784 180
Fragments arranged by size:
1784 180 94
BsmI GAATG_Cn'
Cuts at: 0 852 2058
Size: 852 1206
Bsp1286I G_dGCh'C
Cuts at: 0 54 94 1205 1780 1878 2058
Size: 54 40 1111 575 98 180
Fragments arranged by size:
1111 575 180 98 54 40
BspHI T'CATG_A
Cuts at: 0 1412 2058
Size: 1412 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 1154 2058
Size: 68 1086 904
Fragments arranged by size:
1086 904 68
BsrFI r'CCGG_y
Cuts at: 0 46 490 2058
Size: 46 444 1568
Fragments arranged by size:
1568 444 46
BsrGI T'GTAC_A
Cuts at: 0 1546 1661 2058
Size: 1546 115 397
Fragments arranged by size:
1546 397 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 2058
Size: 36 2022
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1768 2058
Size: 1768 290
BstYI r'GATC_y
Cuts at: 0 285 755 1038 1325 2058
Size: 285 470 283 287 733
Fragments arranged by size:
733 470 287 285 283
BtgI C'CryG_G
Cuts at: 0 127 716 725 893 1010 2058
Size: 127 589 9 168 117 1048
Fragments arranged by size:
1048 589 168 127 117 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 2058
Size: 447 1611
BtsI GCAGTG_nn'
Cuts at: 0 975 1740 2058
Size: 975 765 318
Fragments arranged by size:
975 765 318
DraI TTT'AAA
Cuts at: 0 137 1265 2058
Size: 137 1128 793
Fragments arranged by size:
1128 793 137
EarI CTCTTCn'nnn_
Cuts at: 0 80 1295 1817 1839 2058
Size: 80 1215 522 22 219
Fragments arranged by size:
1215 522 219 80 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1136 1478 1538 2058
Size: 347 789 342 60 520
Fragments arranged by size:
789 520 347 342 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 865 1721 2058
Size: 814 51 856 337
Fragments arranged by size:
856 814 337 51
EcoO109I rG'GnC_Cy
Cuts at: 0 71 1202 1984 2058
Size: 71 1131 782 74
Fragments arranged by size:
1131 782 74 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1782 1814 2058
Size: 265 32 1485 32 244
Fragments arranged by size:
1485 265 244 32 32
HincII GTy'rAC
Cuts at: 0 713 2058
Size: 713 1345
HindIII A'AGCT_T
Cuts at: 0 327 2058
Size: 327 1731
MfeI C'AATT_G
Cuts at: 0 1474 2058
Size: 1474 584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1057 1319 2058
Size: 555 502 262 739
Fragments arranged by size:
739 555 502 262
MslI CAynn'nnrTG
Cuts at: 0 160 530 655 1766 2058
Size: 160 370 125 1111 292
Fragments arranged by size:
1111 370 292 160 125
MspA1I CmG'CkG
Cuts at: 0 67 297 371 548 1022 1580 1892 2058
Size: 67 230 74 177 474 558 312 166
Fragments arranged by size:
558 474 312 230 177 166 74 67
NcoI C'CATG_G
Cuts at: 0 725 893 2058
Size: 725 168 1165
Fragments arranged by size:
1165 725 168
NdeI CA'TA_TG
Cuts at: 0 1679 2058
Size: 1679 379
NheI G'CTAG_C
Cuts at: 0 1149 2058
Size: 1149 909
NspI r_CATG'y
Cuts at: 0 654 1107 1662 1767 2058
Size: 654 453 555 105 291
Fragments arranged by size:
654 555 453 291 105
PciI A'CATG_T
Cuts at: 0 650 1103 1658 1763 2058
Size: 650 453 555 105 295
Fragments arranged by size:
650 555 453 295 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 690 2058
Size: 690 1368
PfoI T'CCnGG_A
Cuts at: 0 683 2058
Size: 683 1375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 2058
Size: 859 32 1167
Fragments arranged by size:
1167 859 32
PpuMI rG'GwC_Cy
Cuts at: 0 71 1984 2058
Size: 71 1913 74
Fragments arranged by size:
1913 74 71
PspOMI G'GGCC_C
Cuts at: 0 1201 2058
Size: 1201 857
PstI C_TGCA'G
Cuts at: 0 41 2058
Size: 41 2017
PvuII CAG'CTG
Cuts at: 0 297 371 1022 1580 1892 2058
Size: 297 74 651 558 312 166
Fragments arranged by size:
651 558 312 297 166 74
SfcI C'TryA_G
Cuts at: 0 37 1127 2058
Size: 37 1090 931
Fragments arranged by size:
1090 931 37
SgrAI Cr'CCGG_yG
Cuts at: 0 46 2058
Size: 46 2012
StuI AGG'CCT
Cuts at: 0 1672 1716 2058
Size: 1672 44 342
Fragments arranged by size:
1672 342 44
StyI C'CwwG_G
Cuts at: 0 725 893 929 1348 1400 2058
Size: 725 168 36 419 52 658
Fragments arranged by size:
725 658 419 168 52 36
TatI w'GTAC_w
Cuts at: 0 435 1525 1546 1661 2058
Size: 435 1090 21 115 397
Fragments arranged by size:
1090 435 397 115 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1833 1865 2058
Size: 1833 32 193
Fragments arranged by size:
1833 193 32
Tth111I GACn'n_nGTC
Cuts at: 0 775 2058
Size: 775 1283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 1355 1684 2058
Size: 723 632 329 374
Fragments arranged by size:
723 632 374 329
XmnI GAAnn'nnTTC
Cuts at: 0 679 1925 2058
Size: 679 1246 133
Fragments arranged by size:
1246 679 133
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BglI BglII
Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI DraI EarI Eco57MI EcoNI EcoO109I Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NdeI NheI
NspI PciI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SfcI SgrAI StuI StyI TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BclI BfrBI BlpI BmgBI BplI BpuEI
BsaBI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NgoMIV NotI NruI NsiI
PacI PasI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XhoI
XmaI ZraI