Restriction map for
pF1KB8615
(Linear) (Six-base) MAPSORT of: KIBB8615.seq Check: 575 from: 1 to: 1464
KIBB8615.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1004 1464
Size: 841 163 460
Fragments arranged by size:
841 460 163
AhdI GACnn_n'nnGTC
Cuts at: 0 342 653 1464
Size: 342 311 811
Fragments arranged by size:
811 342 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 913 1346 1464
Size: 913 433 118
Fragments arranged by size:
913 433 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 856 888 1464
Size: 856 32 576
Fragments arranged by size:
856 576 32
AlwNI CAG_nnn'CTG
Cuts at: 0 355 1464
Size: 355 1109
ApaI G_GGCC'C
Cuts at: 0 281 1464
Size: 281 1183
AseI AT'TA_AT
Cuts at: 0 601 1464
Size: 601 863
AvaI C'yCGr_G
Cuts at: 0 781 1285 1464
Size: 781 504 179
Fragments arranged by size:
781 504 179
BanI G'GyrC_C
Cuts at: 0 1246 1464
Size: 1246 218
BanII G_rGCy'C
Cuts at: 0 281 782 1464
Size: 281 501 682
Fragments arranged by size:
682 501 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 1212 1285 1464
Size: 1212 73 179
Fragments arranged by size:
1212 179 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1322 1356 1464
Size: 1322 34 108
Fragments arranged by size:
1322 108 34
BglII A'GATC_T
Cuts at: 0 38 395 1464
Size: 38 357 1069
Fragments arranged by size:
1069 357 38
Bme1580I G_kGCm'C
Cuts at: 0 281 1464
Size: 281 1183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1183 1464
Size: 1183 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 861 1464
Size: 427 434 603
Fragments arranged by size:
603 434 427
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 790 1464
Size: 790 674
BseYI C'CCAG_C
Cuts at: 0 1024 1464
Size: 1024 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 1464
Size: 465 999
BsiHKAI G_wGCw'C
Cuts at: 0 98 782 1464
Size: 98 684 682
Fragments arranged by size:
684 682 98
BsmI GAATG_Cn'
Cuts at: 0 918 1464
Size: 918 546
Bsp1286I G_dGCh'C
Cuts at: 0 98 281 782 1464
Size: 98 183 501 682
Fragments arranged by size:
682 501 183 98
BspHI T'CATG_A
Cuts at: 0 509 1464
Size: 509 955
BsrFI r'CCGG_y
Cuts at: 0 1056 1464
Size: 1056 408
BssSI C'ACGA_G
Cuts at: 0 383 1401 1464
Size: 383 1018 63
Fragments arranged by size:
1018 383 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1394 1464
Size: 1394 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1464
Size: 705 759
BstYI r'GATC_y
Cuts at: 0 38 395 1075 1464
Size: 38 357 680 389
Fragments arranged by size:
680 389 357 38
Bsu36I CC'TnA_GG
Cuts at: 0 269 1464
Size: 269 1195
BtgI C'CryG_G
Cuts at: 0 635 1464
Size: 635 829
BtsI GCAGTG_nn'
Cuts at: 0 156 1158 1464
Size: 156 1002 306
Fragments arranged by size:
1002 306 156
DraIII CAC_nnn'GTG
Cuts at: 0 430 1464
Size: 430 1034
EaeI y'GGCC_r
Cuts at: 0 722 1058 1464
Size: 722 336 406
Fragments arranged by size:
722 406 336
EcoICRI GAG'CTC
Cuts at: 0 780 1464
Size: 780 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1004 1464
Size: 841 163 460
Fragments arranged by size:
841 460 163
EcoNI CCTnn'n_nnAGG
Cuts at: 0 272 1464
Size: 272 1192
EcoO109I rG'GnC_Cy
Cuts at: 0 277 278 1290 1352 1464
Size: 277 1 1012 62 112
Fragments arranged by size:
1012 277 112 62 1
EcoRV GAT'ATC
Cuts at: 0 502 1464
Size: 502 962
FspI TGC'GCA
Cuts at: 0 917 1464
Size: 917 547
FspAI rTGC'GCAy
Cuts at: 0 917 1464
Size: 917 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 491 523 639 671 1086 1118
Size: 328 32 131 32 116 32 415 32
Cuts at: 1118 1464
Size: 346
Fragments arranged by size:
415 346 328 131 116 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1464
Size: 123 1341
MslI CAynn'nnrTG
Cuts at: 0 23 1464
Size: 23 1441
MspA1I CmG'CkG
Cuts at: 0 720 819 1464
Size: 720 99 645
Fragments arranged by size:
720 645 99
NaeI GCC'GGC
Cuts at: 0 1058 1464
Size: 1058 406
NcoI C'CATG_G
Cuts at: 0 635 1464
Size: 635 829
NgoMIV G'CCGG_C
Cuts at: 0 1056 1464
Size: 1056 408
PspOMI G'GGCC_C
Cuts at: 0 277 1464
Size: 277 1187
PstI C_TGCA'G
Cuts at: 0 287 892 1335 1464
Size: 287 605 443 129
Fragments arranged by size:
605 443 287 129
PvuII CAG'CTG
Cuts at: 0 720 819 1464
Size: 720 99 645
Fragments arranged by size:
720 645 99
SacI G_AGCT'C
Cuts at: 0 782 1464
Size: 782 682
SfcI C'TryA_G
Cuts at: 0 283 888 1331 1464
Size: 283 605 443 133
Fragments arranged by size:
605 443 283 133
SmlI C'TyrA_G
Cuts at: 0 406 876 1464
Size: 406 470 588
Fragments arranged by size:
588 470 406
SspI AAT'ATT
Cuts at: 0 450 1464
Size: 450 1014
StuI AGG'CCT
Cuts at: 0 267 1464
Size: 267 1197
StyI C'CwwG_G
Cuts at: 0 635 1268 1464
Size: 635 633 196
Fragments arranged by size:
635 633 196
Enzymes that do cut:
AcuI AhdI AlfI AloI AlwNI ApaI AseI AvaI
BanI BanII BbsI BcgI BglII Bme1580I BmrI BpuEI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI FspAI
Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV PspOMI
PstI PvuII SacI SfcI SmlI SspI StuI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI ApaLI ApoI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EarI EciI EcoRI
FalI FseI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI