Restriction map for
pF1KB8617
(Linear) (Six-base) MAPSORT of: KIBB8617.seq Check: 3980 from: 1 to: 1857
KIBB8617.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 500 1857
Size: 500 1357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 281 514 1857
Size: 79 202 233 1343
Fragments arranged by size:
1343 233 202 79
AfeI AGC'GCT
Cuts at: 0 295 1857
Size: 295 1562
AflIII A'CryG_T
Cuts at: 0 963 1857
Size: 963 894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 560 592 1857
Size: 560 32 1265
Fragments arranged by size:
1265 560 32
AlwNI CAG_nnn'CTG
Cuts at: 0 564 745 1194 1857
Size: 564 181 449 663
Fragments arranged by size:
663 564 449 181
ApoI r'AATT_y
Cuts at: 0 569 1304 1728 1857
Size: 569 735 424 129
Fragments arranged by size:
735 569 424 129
AvaI C'yCGr_G
Cuts at: 0 229 1857
Size: 229 1628
BamHI G'GATC_C
Cuts at: 0 1702 1857
Size: 1702 155
BanI G'GyrC_C
Cuts at: 0 672 1857
Size: 672 1185
BanII G_rGCy'C
Cuts at: 0 269 1021 1857
Size: 269 752 836
Fragments arranged by size:
836 752 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 542 1280 1857
Size: 95 447 738 577
Fragments arranged by size:
738 577 447 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 1857
Size: 220 34 1603
Fragments arranged by size:
1603 220 34
BclI T'GATC_A
Cuts at: 0 1532 1857
Size: 1532 325
BglI GCCn_nnn'nGGC
Cuts at: 0 197 1857
Size: 197 1660
BglII A'GATC_T
Cuts at: 0 13 1371 1857
Size: 13 1358 486
Fragments arranged by size:
1358 486 13
BmtI G_CTAG'C
Cuts at: 0 1183 1857
Size: 1183 674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 827 1857
Size: 827 1030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1241 1319 1857
Size: 1241 78 538
Fragments arranged by size:
1241 538 78
Bpu10I CC'TnA_GC
Cuts at: 0 750 1857
Size: 750 1107
BseYI C'CCAG_C
Cuts at: 0 187 296 564 1278 1857
Size: 187 109 268 714 579
Fragments arranged by size:
714 579 268 187 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1857
Size: 758 1099
BsiHKAI G_wGCw'C
Cuts at: 0 512 1021 1504 1857
Size: 512 509 483 353
Fragments arranged by size:
512 509 483 353
BsmI GAATG_Cn'
Cuts at: 0 39 492 1857
Size: 39 453 1365
Fragments arranged by size:
1365 453 39
Bsp1286I G_dGCh'C
Cuts at: 0 269 512 1021 1504 1857
Size: 269 243 509 483 353
Fragments arranged by size:
509 483 353 269 243
BspHI T'CATG_A
Cuts at: 0 1743 1857
Size: 1743 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1597 1857
Size: 1597 260
BstYI r'GATC_y
Cuts at: 0 13 1371 1702 1857
Size: 13 1358 331 155
Fragments arranged by size:
1358 331 155 13
Bsu36I CC'TnA_GG
Cuts at: 0 632 1857
Size: 632 1225
DraI TTT'AAA
Cuts at: 0 1310 1857
Size: 1310 547
EaeI y'GGCC_r
Cuts at: 0 198 731 1857
Size: 198 533 1126
Fragments arranged by size:
1126 533 198
EarI CTCTTCn'nnn_
Cuts at: 0 257 422 714 1596 1672 1747 1857
Size: 257 165 292 882 76 75 110
Fragments arranged by size:
882 292 257 165 110 76 75
EcoICRI GAG'CTC
Cuts at: 0 1019 1857
Size: 1019 838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 281 514 1241 1319 1857
Size: 79 202 233 727 78 538
Fragments arranged by size:
727 538 233 202 79 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1857
Size: 1341 516
HaeII r_GCGC'y
Cuts at: 0 297 1857
Size: 297 1560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 460 492 534 566 733 765
Size: 335 32 93 32 42 32 167 32
Cuts at: 765 1857
Size: 1092
Fragments arranged by size:
1092 335 167 93 42 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1186 1857
Size: 80 1106 671
Fragments arranged by size:
1106 671 80
MscI TGG'CCA
Cuts at: 0 200 733 1857
Size: 200 533 1124
Fragments arranged by size:
1124 533 200
MslI CAynn'nnrTG
Cuts at: 0 688 1857
Size: 688 1169
MspA1I CmG'CkG
Cuts at: 0 564 763 1857
Size: 564 199 1094
Fragments arranged by size:
1094 564 199
NheI G'CTAG_C
Cuts at: 0 1179 1857
Size: 1179 678
NspI r_CATG'y
Cuts at: 0 967 1857
Size: 967 890
PasI CC'CwG_GG
Cuts at: 0 988 1857
Size: 988 869
PciI A'CATG_T
Cuts at: 0 963 1857
Size: 963 894
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1620 1652 1857
Size: 1620 32 205
Fragments arranged by size:
1620 205 32
PvuII CAG'CTG
Cuts at: 0 564 763 1857
Size: 564 199 1094
Fragments arranged by size:
1094 564 199
SacI G_AGCT'C
Cuts at: 0 1021 1857
Size: 1021 836
SapI GCTCTTCn'nnn_
Cuts at: 0 257 1857
Size: 257 1600
SfcI C'TryA_G
Cuts at: 0 131 137 1022 1857
Size: 131 6 885 835
Fragments arranged by size:
885 835 131 6
StuI AGG'CCT
Cuts at: 0 1523 1857
Size: 1523 334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1077 1857
Size: 1077 780
XmnI GAAnn'nnTTC
Cuts at: 0 92 1118 1388 1857
Size: 92 1026 270 469
Fragments arranged by size:
1026 469 270 92
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BcgI BclI BglI BglII
BmtI BplI BpmI Bpu10I BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BstXI BstYI Bsu36I DraI EaeI EarI
EcoICRI Eco57MI FalI HaeII Hin4I MmeI MscI MslI
MspA1I NheI NspI PasI PciI PsrI PvuII SacI
SapI SfcI StuI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BfrBI BlpI Bme1580I BmgBI BmrI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI ZraI