(Linear) (Six-base) MAPSORT of: KIBB8620.seq  Check: 4347  from: 1  to: 552



KIBB8620.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AleI CACnn'nnGTG

Cuts at:      0     147     552 
   Size:        147     405
 
AlwNI CAG_nnn'CTG

Cuts at:      0     514     552 
   Size:        514      38
 
ApoI r'AATT_y

Cuts at:      0      77     552 
   Size:         77     475
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     552 
   Size:        162     390
 
BglII A'GATC_T

Cuts at:      0     467     552 
   Size:        467      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     552 
   Size:        491      61
 
Bpu10I CC'TnA_GC

Cuts at:      0      30     552 
   Size:         30     522
 
BsmI GAATG_Cn'

Cuts at:      0     424     552 
   Size:        424     128
 
BsrDI GCAATG_nn'

Cuts at:      0     203     552 
   Size:        203     349
 
BsrGI T'GTAC_A

Cuts at:      0      60     552 
   Size:         60     492
 
BstYI r'GATC_y

Cuts at:      0     467     552 
   Size:        467      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     552 
   Size:        491      61
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     372     552 
   Size:        372     180
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     552 
   Size:         21     531
 
MslI CAynn'nnrTG

Cuts at:      0     147     552 
   Size:        147     405
 
MspA1I CmG'CkG

Cuts at:      0     546     552 
   Size:        546       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     514     552 
   Size:        514      38
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      53      85     552 
   Size:         53      32     467

  Fragments arranged by size: 

                 467      53      32
 
PstI C_TGCA'G

Cuts at:      0     177     552 
   Size:        177     375
 
PvuII CAG'CTG

Cuts at:      0     546     552 
   Size:        546       6
 
SfcI C'TryA_G

Cuts at:      0     173     552 
   Size:        173     379
 
TatI w'GTAC_w

Cuts at:      0      60     552 
   Size:         60     492
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     405     437     552 
   Size:        405      32     115

  Fragments arranged by size: 

                 405     115      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     296     552 
   Size:        296     256
 

 Enzymes that do cut:

     AleI    AlwNI     ApoI    BciVI    BglII     BpmI   Bpu10I     BsmI
    BsrDI    BsrGI    BstYI  Eco57MI     FalI     MmeI     MslI   MspA1I
    PflMI     PsrI     PstI    PvuII     SfcI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI