Restriction map for
pF1KB8627
(Linear) (Six-base) MAPSORT of: KIBB8627.seq Check: 5622 from: 1 to: 2322
KIBB8627.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 1638 2322
Size: 1638 684
AclI AA'CG_TT
Cuts at: 0 595 2322
Size: 595 1727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 496 773 1228 1407 1582 2322
Size: 229 267 277 455 179 175 740
Fragments arranged by size:
740 455 277 267 229 179 175
AfeI AGC'GCT
Cuts at: 0 2301 2322
Size: 2301 21
AflII C'TTAA_G
Cuts at: 0 1138 2322
Size: 1138 1184
AleI CACnn'nnGTG
Cuts at: 0 1762 2322
Size: 1762 560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1735 2051 2322
Size: 1735 316 271
Fragments arranged by size:
1735 316 271
AlwNI CAG_nnn'CTG
Cuts at: 0 1324 2127 2322
Size: 1324 803 195
Fragments arranged by size:
1324 803 195
ApoI r'AATT_y
Cuts at: 0 379 1936 2322
Size: 379 1557 386
Fragments arranged by size:
1557 386 379
AvaI C'yCGr_G
Cuts at: 0 112 127 1310 2202 2322
Size: 112 15 1183 892 120
Fragments arranged by size:
1183 892 120 112 15
BanI G'GyrC_C
Cuts at: 0 254 2027 2322
Size: 254 1773 295
Fragments arranged by size:
1773 295 254
BanII G_rGCy'C
Cuts at: 0 1560 1742 1856 2322
Size: 1560 182 114 466
Fragments arranged by size:
1560 466 182 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 349 2322
Size: 349 1973
BbvCI CC'TCA_GC
Cuts at: 0 86 2322
Size: 86 2236
BglI GCCn_nnn'nGGC
Cuts at: 0 1669 1963 2322
Size: 1669 294 359
Fragments arranged by size:
1669 359 294
BglII A'GATC_T
Cuts at: 0 453 1896 2322
Size: 453 1443 426
Fragments arranged by size:
1443 453 426
Bme1580I G_kGCm'C
Cuts at: 0 1959 2203 2209 2322
Size: 1959 244 6 113
Fragments arranged by size:
1959 244 113 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 847 1582 2322
Size: 847 735 740
Fragments arranged by size:
847 740 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1835 2184 2322
Size: 470 1365 349 138
Fragments arranged by size:
1365 470 349 138
Bpu10I CC'TnA_GC
Cuts at: 0 86 2322
Size: 86 2236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1408 2322
Size: 1012 396 914
Fragments arranged by size:
1012 914 396
BsaBI GATnn'nnATC
Cuts at: 0 941 1492 2322
Size: 941 551 830
Fragments arranged by size:
941 830 551
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1486 2322
Size: 130 1356 836
Fragments arranged by size:
1356 836 130
BseYI C'CCAG_C
Cuts at: 0 247 1525 1664 1958 2322
Size: 247 1278 139 294 364
Fragments arranged by size:
1278 364 294 247 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1741 2322
Size: 1741 581
BsiHKAI G_wGCw'C
Cuts at: 0 954 1742 2245 2322
Size: 954 788 503 77
Fragments arranged by size:
954 788 503 77
BsmI GAATG_Cn'
Cuts at: 0 367 1276 2322
Size: 367 909 1046
Fragments arranged by size:
1046 909 367
Bsp1286I G_dGCh'C
Cuts at: 0 954 1560 1742 1856 1959 2203 2209 2245
Size: 954 606 182 114 103 244 6 36
Cuts at: 2245 2322
Size: 77
Fragments arranged by size:
954 606 244 182 114 103 77 36
6
BspHI T'CATG_A
Cuts at: 0 1086 2322
Size: 1086 1236
BsrFI r'CCGG_y
Cuts at: 0 100 2322
Size: 100 2222
BsrGI T'GTAC_A
Cuts at: 0 876 2322
Size: 876 1446
BssSI C'ACGA_G
Cuts at: 0 1762 2322
Size: 1762 560
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1544 2213 2322
Size: 1544 669 109
Fragments arranged by size:
1544 669 109
BstBI TT'CG_AA
Cuts at: 0 360 2322
Size: 360 1962
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 2322
Size: 468 1854
BstYI r'GATC_y
Cuts at: 0 108 453 942 1896 2322
Size: 108 345 489 954 426
Fragments arranged by size:
954 489 426 345 108
BstZ17I GTA'TAC
Cuts at: 0 1639 2322
Size: 1639 683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 106 2322
Size: 106 2216
BtsI GCAGTG_nn'
Cuts at: 0 895 1959 2322
Size: 895 1064 363
Fragments arranged by size:
1064 895 363
DraI TTT'AAA
Cuts at: 0 1575 2322
Size: 1575 747
EaeI y'GGCC_r
Cuts at: 0 1670 2322
Size: 1670 652
EarI CTCTTCn'nnn_
Cuts at: 0 1558 2322
Size: 1558 764
EcoICRI GAG'CTC
Cuts at: 0 1740 2322
Size: 1740 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 470 496 773 1228 1407 1582 1835
Size: 229 241 26 277 455 179 175 253
Cuts at: 1835 2184 2322
Size: 349 138
Fragments arranged by size:
455 349 277 253 241 229 179 175
138 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1468 2322
Size: 1468 854
EcoO109I rG'GnC_Cy
Cuts at: 0 60 475 1965 2322
Size: 60 415 1490 357
Fragments arranged by size:
1490 415 357 60
EcoRV GAT'ATC
Cuts at: 0 1511 2322
Size: 1511 811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 916 1402 2322
Size: 916 486 920
Fragments arranged by size:
920 916 486
HaeII r_GCGC'y
Cuts at: 0 2163 2303 2322
Size: 2163 140 19
Fragments arranged by size:
2163 140 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 1391 1423 2322
Size: 1010 32 349 32 899
Fragments arranged by size:
1010 899 349 32 32
HindIII A'AGCT_T
Cuts at: 0 1294 1578 2322
Size: 1294 284 744
Fragments arranged by size:
1294 744 284
MscI TGG'CCA
Cuts at: 0 1672 2322
Size: 1672 650
MslI CAynn'nnrTG
Cuts at: 0 971 1717 1762 2033 2322
Size: 971 746 45 271 289
Fragments arranged by size:
971 746 289 271 45
MspA1I CmG'CkG
Cuts at: 0 85 195 251 1668 2322
Size: 85 110 56 1417 654
Fragments arranged by size:
1417 654 110 85 56
NdeI CA'TA_TG
Cuts at: 0 972 2322
Size: 972 1350
NspI r_CATG'y
Cuts at: 0 2014 2322
Size: 2014 308
PasI CC'CwG_GG
Cuts at: 0 2077 2322
Size: 2077 245
PshAI GACnn'nnGTC
Cuts at: 0 674 2322
Size: 674 1648
PstI C_TGCA'G
Cuts at: 0 308 2322
Size: 308 2014
PvuII CAG'CTG
Cuts at: 0 85 195 251 1668 2322
Size: 85 110 56 1417 654
Fragments arranged by size:
1417 654 110 85 56
SacI G_AGCT'C
Cuts at: 0 1742 2322
Size: 1742 580
ScaI AGT'ACT
Cuts at: 0 1241 2322
Size: 1241 1081
SfcI C'TryA_G
Cuts at: 0 304 662 2322
Size: 304 358 1660
Fragments arranged by size:
1660 358 304
SmaI CCC'GGG
Cuts at: 0 1312 2204 2322
Size: 1312 892 118
Fragments arranged by size:
1312 892 118
SmlI C'TyrA_G
Cuts at: 0 112 1027 1138 1387 2322
Size: 112 915 111 249 935
Fragments arranged by size:
935 915 249 112 111
SphI G_CATG'C
Cuts at: 0 2014 2322
Size: 2014 308
SrfI GCCC'GGGC
Cuts at: 0 2204 2322
Size: 2204 118
StuI AGG'CCT
Cuts at: 0 1450 1728 2116 2312 2322
Size: 1450 278 388 196 10
Fragments arranged by size:
1450 388 278 196 10
StyI C'CwwG_G
Cuts at: 0 716 1682 1794 2322
Size: 716 966 112 528
Fragments arranged by size:
966 716 528 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1265 2322
Size: 37 1228 1057
Fragments arranged by size:
1228 1057 37
TatI w'GTAC_w
Cuts at: 0 876 1239 2322
Size: 876 363 1083
Fragments arranged by size:
1083 876 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2322
Size: 1051 32 1239
Fragments arranged by size:
1239 1051 32
Tth111I GACn'n_nGTC
Cuts at: 0 1082 2322
Size: 1082 1240
XbaI T'CTAG_A
Cuts at: 0 1173 2322
Size: 1173 1149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 248 1047 1680 2322
Size: 248 799 633 642
Fragments arranged by size:
799 642 633 248
XhoI C'TCGA_G
Cuts at: 0 112 2322
Size: 112 2210
XmaI C'CCGG_G
Cuts at: 0 1310 2202 2322
Size: 1310 892 120
Fragments arranged by size:
1310 892 120
XmnI GAAnn'nnTTC
Cuts at: 0 1241 2322
Size: 1241 1081
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AleI AlfI AlwNI
ApoI AvaI BanI BanII BbsI BbvCI BglI BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HindIII MscI MslI MspA1I NdeI NspI
PasI PshAI PstI PvuII SacI ScaI SfcI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflIII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BsaI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BssHII BstEII Bsu36I BtgI
ClaI CspCI DraIII DrdI EagI EciI EcoRI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SspI SwaI ZraI