Restriction map for
pF1KB8628
(Linear) (Six-base) MAPSORT of: KIBB8628.seq Check: 3827 from: 1 to: 2523
KIBB8628.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 768 777 2523
Size: 768 9 1746
Fragments arranged by size:
1746 768 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 821 1951 2281 2410 2523
Size: 622 199 1130 330 129 113
Fragments arranged by size:
1130 622 330 199 129 113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1350 1382 2523
Size: 1350 32 1141
Fragments arranged by size:
1350 1141 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2304 2523
Size: 2304 219
ApoI r'AATT_y
Cuts at: 0 374 415 1688 2233 2523
Size: 374 41 1273 545 290
Fragments arranged by size:
1273 545 374 290 41
AseI AT'TA_AT
Cuts at: 0 2407 2523
Size: 2407 116
AvaI C'yCGr_G
Cuts at: 0 1645 2523
Size: 1645 878
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1585 1618 2523
Size: 1585 33 905
Fragments arranged by size:
1585 905 33
BamHI G'GATC_C
Cuts at: 0 831 2523
Size: 831 1692
BanII G_rGCy'C
Cuts at: 0 2053 2379 2523
Size: 2053 326 144
Fragments arranged by size:
2053 326 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 2523
Size: 514 2009
BciVI GTATCCnnnnn_n'
Cuts at: 0 381 1586 2523
Size: 381 1205 937
Fragments arranged by size:
1205 937 381
BglII A'GATC_T
Cuts at: 0 564 1068 2523
Size: 564 504 1455
Fragments arranged by size:
1455 564 504
BlpI GC'TnA_GC
Cuts at: 0 2339 2523
Size: 2339 184
BmgBI CAC'GTC
Cuts at: 0 902 2419 2523
Size: 902 1517 104
Fragments arranged by size:
1517 902 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 2314 2523
Size: 2314 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 542 1097 2523
Size: 542 555 1426
Fragments arranged by size:
1426 555 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 2329 2393 2523
Size: 50 2279 64 130
Fragments arranged by size:
2279 130 64 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 2523
Size: 1561 962
BsaI GGTCTCn'nnnn_
Cuts at: 0 1068 1303 2523
Size: 1068 235 1220
Fragments arranged by size:
1220 1068 235
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1070 1399 2523
Size: 1070 329 1124
Fragments arranged by size:
1124 1070 329
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1620 2523
Size: 1620 903
BsiHKAI G_wGCw'C
Cuts at: 0 74 2523
Size: 74 2449
Bsp1286I G_dGCh'C
Cuts at: 0 74 2053 2379 2523
Size: 74 1979 326 144
Fragments arranged by size:
1979 326 144 74
BsrDI GCAATG_nn'
Cuts at: 0 589 1594 2333 2523
Size: 589 1005 739 190
Fragments arranged by size:
1005 739 589 190
BsrGI T'GTAC_A
Cuts at: 0 2264 2523
Size: 2264 259
BstEII G'GTnAC_C
Cuts at: 0 1258 1749 2523
Size: 1258 491 774
Fragments arranged by size:
1258 774 491
BstYI r'GATC_y
Cuts at: 0 564 633 831 1068 2523
Size: 564 69 198 237 1455
Fragments arranged by size:
1455 564 237 198 69
BstZ17I GTA'TAC
Cuts at: 0 769 2523
Size: 769 1754
Bsu36I CC'TnA_GG
Cuts at: 0 2323 2523
Size: 2323 200
BtgI C'CryG_G
Cuts at: 0 396 2054 2523
Size: 396 1658 469
Fragments arranged by size:
1658 469 396
DraI TTT'AAA
Cuts at: 0 500 759 1397 2523
Size: 500 259 638 1126
Fragments arranged by size:
1126 638 500 259
DraIII CAC_nnn'GTG
Cuts at: 0 2046 2523
Size: 2046 477
EaeI y'GGCC_r
Cuts at: 0 621 1983 2523
Size: 621 1362 540
Fragments arranged by size:
1362 621 540
EarI CTCTTCn'nnn_
Cuts at: 0 2096 2105 2378 2523
Size: 2096 9 273 145
Fragments arranged by size:
2096 273 145 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 622 821 1951 2281 2329 2393 2410
Size: 50 572 199 1130 330 48 64 17
Cuts at: 2410 2523
Size: 113
Fragments arranged by size:
1130 572 330 199 113 64 50 48
17
EcoO109I rG'GnC_Cy
Cuts at: 0 976 2523
Size: 976 1547
EcoRI G'AATT_C
Cuts at: 0 415 2523
Size: 415 2108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2242 2274 2523
Size: 2242 32 249
Fragments arranged by size:
2242 249 32
HincII GTy'rAC
Cuts at: 0 890 2523
Size: 890 1633
HindIII A'AGCT_T
Cuts at: 0 207 1059 2523
Size: 207 852 1464
Fragments arranged by size:
1464 852 207
MfeI C'AATT_G
Cuts at: 0 105 666 2523
Size: 105 561 1857
Fragments arranged by size:
1857 561 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 623 677 1557 2523
Size: 225 398 54 880 966
Fragments arranged by size:
966 880 398 225 54
MscI TGG'CCA
Cuts at: 0 1985 2523
Size: 1985 538
MslI CAynn'nnrTG
Cuts at: 0 242 2523
Size: 242 2281
NcoI C'CATG_G
Cuts at: 0 396 2054 2523
Size: 396 1658 469
Fragments arranged by size:
1658 469 396
NspI r_CATG'y
Cuts at: 0 708 888 1290 1980 2485 2523
Size: 708 180 402 690 505 38
Fragments arranged by size:
708 690 505 402 180 38
PasI CC'CwG_GG
Cuts at: 0 1553 2523
Size: 1553 970
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 2083 2523
Size: 402 1681 440
Fragments arranged by size:
1681 440 402
PfoI T'CCnGG_A
Cuts at: 0 972 2370 2507 2523
Size: 972 1398 137 16
Fragments arranged by size:
1398 972 137 16
PpuMI rG'GwC_Cy
Cuts at: 0 976 2523
Size: 976 1547
PshAI GACnn'nnGTC
Cuts at: 0 1952 2523
Size: 1952 571
PstI C_TGCA'G
Cuts at: 0 1586 1878 2523
Size: 1586 292 645
Fragments arranged by size:
1586 645 292
SapI GCTCTTCn'nnn_
Cuts at: 0 2378 2523
Size: 2378 145
SexAI A'CCwGG_T
Cuts at: 0 930 1571 2523
Size: 930 641 952
Fragments arranged by size:
952 930 641
SfcI C'TryA_G
Cuts at: 0 1582 1874 2067 2523
Size: 1582 292 193 456
Fragments arranged by size:
1582 456 292 193
SmaI CCC'GGG
Cuts at: 0 1647 2523
Size: 1647 876
SmlI C'TyrA_G
Cuts at: 0 1540 2523
Size: 1540 983
SpeI A'CTAG_T
Cuts at: 0 1735 2523
Size: 1735 788
SspI AAT'ATT
Cuts at: 0 297 629 1119 1156 1503 1832 2523
Size: 297 332 490 37 347 329 691
Fragments arranged by size:
691 490 347 332 329 297 37
StyI C'CwwG_G
Cuts at: 0 282 330 396 1293 2054 2422 2523
Size: 282 48 66 897 761 368 101
Fragments arranged by size:
897 761 368 282 101 66 48
SwaI ATTT'AAAT
Cuts at: 0 1397 2523
Size: 1397 1126
TatI w'GTAC_w
Cuts at: 0 1970 2264 2411 2523
Size: 1970 294 147 112
Fragments arranged by size:
1970 294 147 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 954 986 2523
Size: 182 32 740 32 1537
Fragments arranged by size:
1537 740 182 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 900 2523
Size: 900 1623
XbaI T'CTAG_A
Cuts at: 0 561 2523
Size: 561 1962
XmaI C'CCGG_G
Cuts at: 0 1645 2523
Size: 1645 878
XmnI GAAnn'nnTTC
Cuts at: 0 1172 2523
Size: 1172 1351
Enzymes that do cut:
AccI AcuI AloI AlwNI ApoI AseI AvaI BaeI
BamHI BanII BbsI BciVI BglII BlpI BmgBI BmrI
BplI BpmI BpuEI BsaI BseRI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstEII BstYI BstZ17I Bsu36I BtgI DraI
DraIII EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MfeI MmeI MscI MslI NcoI NspI PasI
PflMI PfoI PpuMI PshAI PstI SapI SexAI SfcI
SmaI SmlI SpeI SspI StyI SwaI TatI TstI
Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII
BanI BbeI BbvCI BcgI BclI BfrBI BglI Bme1580I
BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BtgZI BtsI
ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI StuI TaqII XcmI XhoI ZraI