Restriction map for
pF1KB8629
(Linear) (Six-base) MAPSORT of: KIBB8629.seq Check: 617 from: 1 to: 1593
KIBB8629.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 1298 1593
Size: 1298 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1135 1593
Size: 1099 36 458
Fragments arranged by size:
1099 458 36
AflIII A'CryG_T
Cuts at: 0 255 887 917 923 992 1593
Size: 255 632 30 6 69 601
Fragments arranged by size:
632 601 255 69 30 6
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 1593
Size: 242 32 1319
Fragments arranged by size:
1319 242 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1105 1593
Size: 1105 488
ApoI r'AATT_y
Cuts at: 0 1087 1465 1552 1593
Size: 1087 378 87 41
Fragments arranged by size:
1087 378 87 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 173 206 1593
Size: 173 33 1387
Fragments arranged by size:
1387 173 33
BanI G'GyrC_C
Cuts at: 0 82 1028 1593
Size: 82 946 565
Fragments arranged by size:
946 565 82
BbeI G_GCGC'C
Cuts at: 0 1032 1593
Size: 1032 561
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 1058 1220 1318 1593
Size: 69 989 162 98 275
Fragments arranged by size:
989 275 162 98 69
BbvCI CC'TCA_GC
Cuts at: 0 87 1593
Size: 87 1506
BclI T'GATC_A
Cuts at: 0 377 1593
Size: 377 1216
BlpI GC'TnA_GC
Cuts at: 0 820 1593
Size: 820 773
Bme1580I G_kGCm'C
Cuts at: 0 917 1593
Size: 917 676
BmgBI CAC'GTC
Cuts at: 0 890 993 1593
Size: 890 103 600
Fragments arranged by size:
890 600 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 1249 1593
Size: 1249 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1258 1593
Size: 188 1070 335
Fragments arranged by size:
1070 335 188
Bpu10I CC'TnA_GC
Cuts at: 0 87 1593
Size: 87 1506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1593
Size: 29 1564
BsaAI yAC'GTr
Cuts at: 0 918 1593
Size: 918 675
BsaBI GATnn'nnATC
Cuts at: 0 1234 1593
Size: 1234 359
BsaHI Gr'CG_yC
Cuts at: 0 1029 1593
Size: 1029 564
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 1593
Size: 500 1093
BseYI C'CCAG_C
Cuts at: 0 611 636 792 825 1593
Size: 611 25 156 33 768
Fragments arranged by size:
768 611 156 33 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1593
Size: 966 627
BsmI GAATG_Cn'
Cuts at: 0 1416 1593
Size: 1416 177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1026 1593
Size: 1026 567
Bsp1286I G_dGCh'C
Cuts at: 0 917 1593
Size: 917 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 824 1310 1593
Size: 824 486 283
Fragments arranged by size:
824 486 283
BsrDI GCAATG_nn'
Cuts at: 0 554 1556 1593
Size: 554 1002 37
Fragments arranged by size:
1002 554 37
BsrGI T'GTAC_A
Cuts at: 0 920 1593
Size: 920 673
BssSI C'ACGA_G
Cuts at: 0 1023 1593
Size: 1023 570
BstBI TT'CG_AA
Cuts at: 0 1122 1593
Size: 1122 471
BtgI C'CryG_G
Cuts at: 0 578 1593
Size: 578 1015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 330 1593
Size: 330 1263
ClaI AT'CG_AT
Cuts at: 0 1238 1593
Size: 1238 355
DraIII CAC_nnn'GTG
Cuts at: 0 696 1593
Size: 696 897
EaeI y'GGCC_r
Cuts at: 0 471 1593
Size: 471 1122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1099 1135 1258 1593
Size: 188 911 36 123 335
Fragments arranged by size:
911 335 188 123 36
EcoRI G'AATT_C
Cuts at: 0 1465 1552 1593
Size: 1465 87 41
Fragments arranged by size:
1465 87 41
HaeII r_GCGC'y
Cuts at: 0 1032 1593
Size: 1032 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 1593
Size: 40 32 1521
Fragments arranged by size:
1521 40 32
HincII GTy'rAC
Cuts at: 0 1299 1593
Size: 1299 294
KasI G'GCGC_C
Cuts at: 0 1028 1593
Size: 1028 565
MscI TGG'CCA
Cuts at: 0 473 1593
Size: 473 1120
MslI CAynn'nnrTG
Cuts at: 0 838 1593
Size: 838 755
MspA1I CmG'CkG
Cuts at: 0 433 636 663 792 1593
Size: 433 203 27 129 801
Fragments arranged by size:
801 433 203 129 27
NarI GG'CG_CC
Cuts at: 0 1029 1593
Size: 1029 564
NcoI C'CATG_G
Cuts at: 0 578 1593
Size: 578 1015
NdeI CA'TA_TG
Cuts at: 0 783 1593
Size: 783 810
NspI r_CATG'y
Cuts at: 0 259 927 1593
Size: 259 668 666
Fragments arranged by size:
668 666 259
PciI A'CATG_T
Cuts at: 0 255 923 1593
Size: 255 668 670
Fragments arranged by size:
670 668 255
PmlI CAC'GTG
Cuts at: 0 918 1593
Size: 918 675
PsiI TTA'TAA
Cuts at: 0 461 1593
Size: 461 1132
PstI C_TGCA'G
Cuts at: 0 897 1437 1518 1593
Size: 897 540 81 75
Fragments arranged by size:
897 540 81 75
PvuII CAG'CTG
Cuts at: 0 433 636 663 792 1593
Size: 433 203 27 129 801
Fragments arranged by size:
801 433 203 129 27
SalI G'TCGA_C
Cuts at: 0 1297 1593
Size: 1297 296
SfcI C'TryA_G
Cuts at: 0 147 491 893 1319 1433 1514 1593
Size: 147 344 402 426 114 81 79
Fragments arranged by size:
426 402 344 147 114 81 79
SfoI GGC'GCC
Cuts at: 0 1030 1593
Size: 1030 563
SmlI C'TyrA_G
Cuts at: 0 44 1593
Size: 44 1549
StyI C'CwwG_G
Cuts at: 0 339 578 1593
Size: 339 239 1015
Fragments arranged by size:
1015 339 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 985 1593
Size: 985 608
TatI w'GTAC_w
Cuts at: 0 181 920 1593
Size: 181 739 673
Fragments arranged by size:
739 673 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 1388 1420 1593
Size: 57 32 1299 32 173
Fragments arranged by size:
1299 173 57 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1294 1593
Size: 1294 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 322 585 1593
Size: 322 263 1008
Fragments arranged by size:
1008 322 263
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI BaeI BanI
BbeI BbsI BbvCI BclI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI
BstBI BtgI BtgZI ClaI DraIII EaeI Eco57MI EcoRI
HaeII Hin4I HincII KasI MscI MslI MspA1I NarI
NcoI NdeI NspI PciI PmlI PsiI PstI PvuII
SalI SfcI SfoI SmlI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanII BcgI BciVI BfrBI BglI BglII
BmtI BplI BsaI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtsI CspCI DraI DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NaeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI
XmnI ZraI