Restriction map for
pF1KB8630
(Linear) (Six-base) MAPSORT of: KIBB8630.seq Check: 6399 from: 1 to: 2508
KIBB8630.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 998 1883 2508
Size: 998 885 625
Fragments arranged by size:
998 885 625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 1550 2508
Size: 1528 22 958
Fragments arranged by size:
1528 958 22
AfeI AGC'GCT
Cuts at: 0 24 2508
Size: 24 2484
AgeI A'CCGG_T
Cuts at: 0 1699 2508
Size: 1699 809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 557 589 1143 1175 2508
Size: 557 32 554 32 1333
Fragments arranged by size:
1333 557 554 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 295 503 2508
Size: 295 208 2005
Fragments arranged by size:
2005 295 208
ApaI G_GGCC'C
Cuts at: 0 2415 2508
Size: 2415 93
ApoI r'AATT_y
Cuts at: 0 1523 2508
Size: 1523 985
AvaI C'yCGr_G
Cuts at: 0 990 2508
Size: 990 1518
AvrII C'CTAG_G
Cuts at: 0 1833 2508
Size: 1833 675
BamHI G'GATC_C
Cuts at: 0 2118 2508
Size: 2118 390
BanI G'GyrC_C
Cuts at: 0 718 2508
Size: 718 1790
BanII G_rGCy'C
Cuts at: 0 35 991 1323 1810 2415 2508
Size: 35 956 332 487 605 93
Fragments arranged by size:
956 605 487 332 93 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 2235 2508
Size: 2235 273
BbvCI CC'TCA_GC
Cuts at: 0 2320 2508
Size: 2320 188
BciVI GTATCCnnnnn_n'
Cuts at: 0 331 2508
Size: 331 2177
BfrBI ATG'CAT
Cuts at: 0 2145 2508
Size: 2145 363
Bme1580I G_kGCm'C
Cuts at: 0 723 2415 2508
Size: 723 1692 93
Fragments arranged by size:
1692 723 93
BmtI G_CTAG'C
Cuts at: 0 2062 2276 2508
Size: 2062 214 232
Fragments arranged by size:
2062 232 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 41 2508
Size: 41 2467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 942 1594 2368 2508
Size: 273 669 652 774 140
Fragments arranged by size:
774 669 652 273 140
Bpu10I CC'TnA_GC
Cuts at: 0 676 2320 2508
Size: 676 1644 188
Fragments arranged by size:
1644 676 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1387 2407 2508
Size: 1336 51 1020 101
Fragments arranged by size:
1336 1020 101 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 2508
Size: 304 2204
BsaWI w'CCGG_w
Cuts at: 0 1699 2508
Size: 1699 809
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 557 587 947 977 2508
Size: 557 30 360 30 1531
Fragments arranged by size:
1531 557 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 787 2508
Size: 436 351 1721
Fragments arranged by size:
1721 436 351
BseYI C'CCAG_C
Cuts at: 0 1154 1473 2508
Size: 1154 319 1035
Fragments arranged by size:
1154 1035 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 2350 2508
Size: 189 2161 158
Fragments arranged by size:
2161 189 158
BsiHKAI G_wGCw'C
Cuts at: 0 1810 2508
Size: 1810 698
BsmI GAATG_Cn'
Cuts at: 0 1012 1047 2508
Size: 1012 35 1461
Fragments arranged by size:
1461 1012 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2182 2508
Size: 2182 326
Bsp1286I G_dGCh'C
Cuts at: 0 35 723 991 1323 1810 2415 2508
Size: 35 688 268 332 487 605 93
Fragments arranged by size:
688 605 487 332 268 93 35
BspHI T'CATG_A
Cuts at: 0 1848 2508
Size: 1848 660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1613 2414 2508
Size: 1613 801 94
Fragments arranged by size:
1613 801 94
BsrDI GCAATG_nn'
Cuts at: 0 1017 2508
Size: 1017 1491
BsrFI r'CCGG_y
Cuts at: 0 194 1699 2508
Size: 194 1505 809
Fragments arranged by size:
1505 809 194
BsrGI T'GTAC_A
Cuts at: 0 1826 1934 2508
Size: 1826 108 574
Fragments arranged by size:
1826 574 108
BstEII G'GTnAC_C
Cuts at: 0 1489 2508
Size: 1489 1019
BstXI CCAn_nnnn'nTGG
Cuts at: 0 235 2508
Size: 235 2273
BstYI r'GATC_y
Cuts at: 0 320 1766 2011 2118 2508
Size: 320 1446 245 107 390
Fragments arranged by size:
1446 390 320 245 107
BstZ17I GTA'TAC
Cuts at: 0 1884 2508
Size: 1884 624
BtgI C'CryG_G
Cuts at: 0 124 2508
Size: 124 2384
BtsI GCAGTG_nn'
Cuts at: 0 401 2508
Size: 401 2107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1913 1948 2508
Size: 1913 35 560
Fragments arranged by size:
1913 560 35
DraI TTT'AAA
Cuts at: 0 1694 2508
Size: 1694 814
DraIII CAC_nnn'GTG
Cuts at: 0 939 2508
Size: 939 1569
EaeI y'GGCC_r
Cuts at: 0 196 1655 2508
Size: 196 1459 853
Fragments arranged by size:
1459 853 196
EarI CTCTTCn'nnn_
Cuts at: 0 167 307 442 979 1504 2101 2508
Size: 167 140 135 537 525 597 407
Fragments arranged by size:
597 537 525 407 167 140 135
EcoICRI GAG'CTC
Cuts at: 0 1808 2508
Size: 1808 700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 942 1528 1550 1594 2368 2508
Size: 273 669 586 22 44 774 140
Fragments arranged by size:
774 669 586 273 140 44 22
EcoO109I rG'GnC_Cy
Cuts at: 0 105 1788 2412 2508
Size: 105 1683 624 96
Fragments arranged by size:
1683 624 105 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1119 2115 2508
Size: 1119 996 393
Fragments arranged by size:
1119 996 393
FspI TGC'GCA
Cuts at: 0 534 2508
Size: 534 1974
HaeII r_GCGC'y
Cuts at: 0 26 1544 2508
Size: 26 1518 964
Fragments arranged by size:
1518 964 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 407 439 947 979 1081 1113
Size: 246 32 129 32 508 32 102 32
Cuts at: 1113 2508
Size: 1395
Fragments arranged by size:
1395 508 246 129 102 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 309 753 2508
Size: 309 444 1755
Fragments arranged by size:
1755 444 309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 2508
Size: 785 1723
MscI TGG'CCA
Cuts at: 0 1657 2508
Size: 1657 851
MslI CAynn'nnrTG
Cuts at: 0 2372 2508
Size: 2372 136
MspA1I CmG'CkG
Cuts at: 0 778 2191 2212 2290 2508
Size: 778 1413 21 78 218
Fragments arranged by size:
1413 778 218 78 21
NcoI C'CATG_G
Cuts at: 0 124 2508
Size: 124 2384
NheI G'CTAG_C
Cuts at: 0 2058 2272 2508
Size: 2058 214 236
Fragments arranged by size:
2058 236 214
NsiI A_TGCA'T
Cuts at: 0 2147 2508
Size: 2147 361
NspI r_CATG'y
Cuts at: 0 286 549 1587 2145 2262 2508
Size: 286 263 1038 558 117 246
Fragments arranged by size:
1038 558 286 263 246 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 503 2508
Size: 503 2005
PfoI T'CCnGG_A
Cuts at: 0 1610 2508
Size: 1610 898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 2508
Size: 557 32 1919
Fragments arranged by size:
1919 557 32
PpuMI rG'GwC_Cy
Cuts at: 0 105 1788 2508
Size: 105 1683 720
Fragments arranged by size:
1683 720 105
PsiI TTA'TAA
Cuts at: 0 1592 1649 2508
Size: 1592 57 859
Fragments arranged by size:
1592 859 57
PspOMI G'GGCC_C
Cuts at: 0 2411 2508
Size: 2411 97
PstI C_TGCA'G
Cuts at: 0 2420 2508
Size: 2420 88
PvuII CAG'CTG
Cuts at: 0 2191 2212 2290 2508
Size: 2191 21 78 218
Fragments arranged by size:
2191 218 78 21
SacI G_AGCT'C
Cuts at: 0 1810 2508
Size: 1810 698
SapI GCTCTTCn'nnn_
Cuts at: 0 979 2101 2508
Size: 979 1122 407
Fragments arranged by size:
1122 979 407
SfcI C'TryA_G
Cuts at: 0 1373 1545 2416 2508
Size: 1373 172 871 92
Fragments arranged by size:
1373 871 172 92
SmlI C'TyrA_G
Cuts at: 0 1351 1402 2422 2508
Size: 1351 51 1020 86
Fragments arranged by size:
1351 1020 86 51
SphI G_CATG'C
Cuts at: 0 286 2508
Size: 286 2222
StuI AGG'CCT
Cuts at: 0 1671 2325 2508
Size: 1671 654 183
Fragments arranged by size:
1671 654 183
StyI C'CwwG_G
Cuts at: 0 124 327 1494 1719 1833 1909 2508
Size: 124 203 1167 225 114 76 599
Fragments arranged by size:
1167 599 225 203 124 114 76
SwaI ATTT'AAAT
Cuts at: 0 1694 2508
Size: 1694 814
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1300 1936 2508
Size: 706 594 636 572
Fragments arranged by size:
706 636 594 572
TatI w'GTAC_w
Cuts at: 0 1559 1826 1934 2508
Size: 1559 267 108 574
Fragments arranged by size:
1559 574 267 108
Tth111I GACn'n_nGTC
Cuts at: 0 1095 2434 2508
Size: 1095 1339 74
Fragments arranged by size:
1339 1095 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 122 1481 2508
Size: 122 1359 1027
Fragments arranged by size:
1359 1027 122
XmnI GAAnn'nnTTC
Cuts at: 0 1895 2508
Size: 1895 613
Enzymes that do cut:
AccI AcuI AfeI AgeI AloI AlwNI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BciVI
BfrBI Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstEII BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NheI NsiI
NspI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI
PvuII SacI SapI SfcI SmlI SphI StuI StyI
SwaI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AleI
AlfI ApaLI AscI AseI AsiSI BaeI BbeI BcgI
BclI BglI BglII BlpI BmgBI BmrI BsaAI BsaBI
BsaHI BsiEI BsiWI BspEI BsrBI BssHII BssSI BstAPI
BstBI Bsu36I BtgZI ClaI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PciI PmeI PmlI PshAI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI TstI XbaI XhoI XmaI ZraI