Restriction map for
pF1KB8634
(Linear) (Six-base) MAPSORT of: KIBB8634.seq Check: 5324 from: 1 to: 1023
KIBB8634.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 352 1023
Size: 352 671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1023
Size: 161 862
AfeI AGC'GCT
Cuts at: 0 967 1023
Size: 967 56
AgeI A'CCGG_T
Cuts at: 0 515 1023
Size: 515 508
AleI CACnn'nnGTG
Cuts at: 0 577 1023
Size: 577 446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 279 311 1023
Size: 279 32 712
Fragments arranged by size:
712 279 32
AlwNI CAG_nnn'CTG
Cuts at: 0 299 720 1023
Size: 299 421 303
Fragments arranged by size:
421 303 299
ApoI r'AATT_y
Cuts at: 0 166 1023
Size: 166 857
AvaI C'yCGr_G
Cuts at: 0 188 1023
Size: 188 835
BanII G_rGCy'C
Cuts at: 0 878 897 1023
Size: 878 19 126
Fragments arranged by size:
878 126 19
BlpI GC'TnA_GC
Cuts at: 0 545 969 1023
Size: 545 424 54
Fragments arranged by size:
545 424 54
BmgBI CAC'GTC
Cuts at: 0 228 1023
Size: 228 795
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 930 1023
Size: 930 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 254 1023
Size: 88 166 769
Fragments arranged by size:
769 166 88
Bpu10I CC'TnA_GC
Cuts at: 0 94 859 880 1023
Size: 94 765 21 143
Fragments arranged by size:
765 143 94 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 925 1023
Size: 331 594 98
Fragments arranged by size:
594 331 98
BsaBI GATnn'nnATC
Cuts at: 0 440 616 1023
Size: 440 176 407
Fragments arranged by size:
440 407 176
BsaWI w'CCGG_w
Cuts at: 0 44 515 1023
Size: 44 471 508
Fragments arranged by size:
508 471 44
BseYI C'CCAG_C
Cuts at: 0 1008 1023
Size: 1008 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1023
Size: 998 25
BsiEI CG_ry'CG
Cuts at: 0 138 1023
Size: 138 885
BsmI GAATG_Cn'
Cuts at: 0 57 1023
Size: 57 966
Bsp1286I G_dGCh'C
Cuts at: 0 878 897 1023
Size: 878 19 126
Fragments arranged by size:
878 126 19
BspEI T'CCGG_A
Cuts at: 0 44 1023
Size: 44 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 1023
Size: 128 895
BsrFI r'CCGG_y
Cuts at: 0 77 515 1023
Size: 77 438 508
Fragments arranged by size:
508 438 77
BssSI C'ACGA_G
Cuts at: 0 15 1023
Size: 15 1008
BstBI TT'CG_AA
Cuts at: 0 457 1023
Size: 457 566
BstYI r'GATC_y
Cuts at: 0 716 952 1023
Size: 716 236 71
Fragments arranged by size:
716 236 71
BstZ17I GTA'TAC
Cuts at: 0 353 1023
Size: 353 670
EaeI y'GGCC_r
Cuts at: 0 79 626 1023
Size: 79 547 397
Fragments arranged by size:
547 397 79
EarI CTCTTCn'nnn_
Cuts at: 0 256 1023
Size: 256 767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 161 254 1023
Size: 88 73 93 769
Fragments arranged by size:
769 93 88 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 925 1023
Size: 925 98
EcoO109I rG'GnC_Cy
Cuts at: 0 402 942 1023
Size: 402 540 81
Fragments arranged by size:
540 402 81
EcoRI G'AATT_C
Cuts at: 0 166 1023
Size: 166 857
EcoRV GAT'ATC
Cuts at: 0 618 1023
Size: 618 405
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1023
Size: 267 756
HaeII r_GCGC'y
Cuts at: 0 969 1023
Size: 969 54
HincII GTy'rAC
Cuts at: 0 337 1023
Size: 337 686
HindIII A'AGCT_T
Cuts at: 0 901 1023
Size: 901 122
MslI CAynn'nnrTG
Cuts at: 0 577 733 1023
Size: 577 156 290
Fragments arranged by size:
577 290 156
MspA1I CmG'CkG
Cuts at: 0 549 1023
Size: 549 474
PflMI CCAn_nnn'nTGG
Cuts at: 0 677 1023
Size: 677 346
PfoI T'CCnGG_A
Cuts at: 0 270 1023
Size: 270 753
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1023
Size: 30 993
PpuMI rG'GwC_Cy
Cuts at: 0 402 942 1023
Size: 402 540 81
Fragments arranged by size:
540 402 81
PstI C_TGCA'G
Cuts at: 0 125 782 1023
Size: 125 657 241
Fragments arranged by size:
657 241 125
PvuII CAG'CTG
Cuts at: 0 549 1023
Size: 549 474
SanDI GG'GwC_CC
Cuts at: 0 402 1023
Size: 402 621
SfcI C'TryA_G
Cuts at: 0 121 778 1023
Size: 121 657 245
Fragments arranged by size:
657 245 121
SmlI C'TyrA_G
Cuts at: 0 346 904 1023
Size: 346 558 119
Fragments arranged by size:
558 346 119
SspI AAT'ATT
Cuts at: 0 203 1023
Size: 203 820
StyI C'CwwG_G
Cuts at: 0 671 869 1023
Size: 671 198 154
Fragments arranged by size:
671 198 154
TatI w'GTAC_w
Cuts at: 0 935 1023
Size: 935 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1023
Size: 573 450
XmnI GAAnn'nnTTC
Cuts at: 0 443 1023
Size: 443 580
Enzymes that do cut:
AccI AcuI AfeI AgeI AleI AloI AlwNI ApoI
AvaI BanII BlpI BmgBI BplI BpmI Bpu10I BpuEI
BsaBI BsaWI BseYI BsgI BsiEI BsmI Bsp1286I BspEI
BspMI BsrFI BssSI BstBI BstYI BstZ17I EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII HincII
HindIII MslI MspA1I PflMI PfoI PpiI PpuMI PstI
PvuII SanDI SfcI SmlI SspI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmrI BmtI BsaI BsaAI BsaHI
BsaXI BseRI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstEII BstXI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI FseI FspI FspAI Hin4I HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI