Restriction map for
pF1KB8635
(Linear) (Six-base) MAPSORT of: KIBB8635.seq Check: 4757 from: 1 to: 723
KIBB8635.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 222 723
Size: 222 501
AflIII A'CryG_T
Cuts at: 0 104 723
Size: 104 619
AleI CACnn'nnGTG
Cuts at: 0 420 723
Size: 420 303
AlwNI CAG_nnn'CTG
Cuts at: 0 86 723
Size: 86 637
ApaI G_GGCC'C
Cuts at: 0 187 723
Size: 187 536
AvaI C'yCGr_G
Cuts at: 0 384 462 723
Size: 384 78 261
Fragments arranged by size:
384 261 78
BanI G'GyrC_C
Cuts at: 0 369 723
Size: 369 354
BanII G_rGCy'C
Cuts at: 0 187 582 723
Size: 187 395 141
Fragments arranged by size:
395 187 141
BbeI G_GCGC'C
Cuts at: 0 373 723
Size: 373 350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 399 723
Size: 365 34 324
Fragments arranged by size:
365 324 34
Bme1580I G_kGCm'C
Cuts at: 0 187 723
Size: 187 536
BmgBI CAC'GTC
Cuts at: 0 105 723
Size: 105 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 204 557 723
Size: 145 59 353 166
Fragments arranged by size:
353 166 145 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 723
Size: 241 482
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 723
Size: 526 197
BsaHI Gr'CG_yC
Cuts at: 0 370 723
Size: 370 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 569 599 723
Size: 28 30 511 30 124
Fragments arranged by size:
511 124 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 622 644 723
Size: 307 315 22 79
Fragments arranged by size:
315 307 79 22
BseYI C'CCAG_C
Cuts at: 0 11 92 526 723
Size: 11 81 434 197
Fragments arranged by size:
434 197 81 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 121 250 258 359 723
Size: 19 102 129 8 101 364
Fragments arranged by size:
364 129 102 101 19 8
BsiEI CG_ry'CG
Cuts at: 0 398 723
Size: 398 325
Bsp1286I G_dGCh'C
Cuts at: 0 187 582 723
Size: 187 395 141
Fragments arranged by size:
395 187 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 222 723
Size: 94 128 501
Fragments arranged by size:
501 128 94
BsrBI CCG'CTC
Cuts at: 0 652 723
Size: 652 71
BsrFI r'CCGG_y
Cuts at: 0 287 540 723
Size: 287 253 183
Fragments arranged by size:
287 253 183
BssHII G'CGCG_C
Cuts at: 0 490 723
Size: 490 233
BssSI C'ACGA_G
Cuts at: 0 292 723
Size: 292 431
BtgI C'CryG_G
Cuts at: 0 355 723
Size: 355 368
DraIII CAC_nnn'GTG
Cuts at: 0 105 723
Size: 105 618
EarI CTCTTCn'nnn_
Cuts at: 0 46 64 723
Size: 46 18 659
Fragments arranged by size:
659 46 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 721 723
Size: 532 189 2
Fragments arranged by size:
532 189 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 204 557 723
Size: 145 59 353 166
Fragments arranged by size:
353 166 145 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 723
Size: 55 668
EcoO109I rG'GnC_Cy
Cuts at: 0 83 114 430 723
Size: 83 31 316 293
Fragments arranged by size:
316 293 83 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 282 723
Size: 189 93 441
Fragments arranged by size:
441 189 93
HaeII r_GCGC'y
Cuts at: 0 373 723
Size: 373 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 723
Size: 445 32 246
Fragments arranged by size:
445 246 32
KasI G'GCGC_C
Cuts at: 0 369 723
Size: 369 354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 723
Size: 384 339
MslI CAynn'nnrTG
Cuts at: 0 420 723
Size: 420 303
NaeI GCC'GGC
Cuts at: 0 289 542 723
Size: 289 253 181
Fragments arranged by size:
289 253 181
NarI GG'CG_CC
Cuts at: 0 370 723
Size: 370 353
NgoMIV G'CCGG_C
Cuts at: 0 287 540 723
Size: 287 253 183
Fragments arranged by size:
287 253 183
PflMI CCAn_nnn'nTGG
Cuts at: 0 212 723
Size: 212 511
PfoI T'CCnGG_A
Cuts at: 0 546 573 723
Size: 546 27 150
Fragments arranged by size:
546 150 27
PpuMI rG'GwC_Cy
Cuts at: 0 83 114 430 723
Size: 83 31 316 293
Fragments arranged by size:
316 293 83 31
PspOMI G'GGCC_C
Cuts at: 0 183 723
Size: 183 540
PstI C_TGCA'G
Cuts at: 0 286 723
Size: 286 437
PvuI CG_AT'CG
Cuts at: 0 398 723
Size: 398 325
SapI GCTCTTCn'nnn_
Cuts at: 0 64 723
Size: 64 659
SfcI C'TryA_G
Cuts at: 0 282 723
Size: 282 441
SfoI GGC'GCC
Cuts at: 0 371 723
Size: 371 352
SmlI C'TyrA_G
Cuts at: 0 256 723
Size: 256 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 423 723
Size: 423 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 130 162 569 601 723
Size: 28 32 70 32 407 32 122
Fragments arranged by size:
407 122 70 32 32 32 28
Enzymes that do cut:
AarI AflIII AleI AlwNI ApaI AvaI BanI BanII
BbeI BcgI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BtgI DraIII EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MslI
NaeI NarI NgoMIV PflMI PfoI PpuMI PspOMI PstI
PvuI SapI SfcI SfoI SmlI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MspA1I NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI