Restriction map for
pF1KB8640
(Linear) (Six-base) MAPSORT of: KIBB8640.seq Check: 1325 from: 1 to: 609
KIBB8640.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 185 609
Size: 185 424
AccI GT'mk_AC
Cuts at: 0 155 275 609
Size: 155 120 334
Fragments arranged by size:
334 155 120
AlwNI CAG_nnn'CTG
Cuts at: 0 405 452 609
Size: 405 47 157
Fragments arranged by size:
405 157 47
AscI GG'CGCG_CC
Cuts at: 0 353 609
Size: 353 256
AvaI C'yCGr_G
Cuts at: 0 36 88 198 205 469 609
Size: 36 52 110 7 264 140
Fragments arranged by size:
264 140 110 52 36 7
BanI G'GyrC_C
Cuts at: 0 31 148 609
Size: 31 117 461
Fragments arranged by size:
461 117 31
BanII G_rGCy'C
Cuts at: 0 205 609
Size: 205 404
BbeI G_GCGC'C
Cuts at: 0 35 152 609
Size: 35 117 457
Fragments arranged by size:
457 117 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 609
Size: 261 348
Bpu10I CC'TnA_GC
Cuts at: 0 233 609
Size: 233 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 609
Size: 183 426
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 609
Size: 331 278
BsaAI yAC'GTr
Cuts at: 0 309 498 609
Size: 309 189 111
Fragments arranged by size:
309 189 111
BsaHI Gr'CG_yC
Cuts at: 0 32 149 182 260 609
Size: 32 117 33 78 349
Fragments arranged by size:
349 117 78 33 32
BsaWI w'CCGG_w
Cuts at: 0 213 609
Size: 213 396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 609
Size: 232 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 609
Size: 536 73
BsiHKAI G_wGCw'C
Cuts at: 0 272 478 609
Size: 272 206 131
Fragments arranged by size:
272 206 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 511 609
Size: 511 98
Bsp1286I G_dGCh'C
Cuts at: 0 205 272 478 609
Size: 205 67 206 131
Fragments arranged by size:
206 205 131 67
BspEI T'CCGG_A
Cuts at: 0 213 609
Size: 213 396
BsrFI r'CCGG_y
Cuts at: 0 98 345 609
Size: 98 247 264
Fragments arranged by size:
264 247 98
BssHII G'CGCG_C
Cuts at: 0 353 423 425 540 542 544 592 609
Size: 353 70 2 115 2 2 48 17
Fragments arranged by size:
353 115 70 48 17 2 2 2
BssSI C'ACGA_G
Cuts at: 0 373 609
Size: 373 236
BstAPI GCAn_nnn'nTGC
Cuts at: 0 405 501 609
Size: 405 96 108
Fragments arranged by size:
405 108 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 609
Size: 375 234
BtgI C'CryG_G
Cuts at: 0 104 131 418 609
Size: 104 27 287 191
Fragments arranged by size:
287 191 104 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 609
Size: 315 294
DrdI GACnn_nn'nnGTC
Cuts at: 0 151 181 609
Size: 151 30 428
Fragments arranged by size:
428 151 30
EaeI y'GGCC_r
Cuts at: 0 20 53 223 431 609
Size: 20 33 170 208 178
Fragments arranged by size:
208 178 170 33 20
EarI CTCTTCn'nnn_
Cuts at: 0 514 609
Size: 514 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 468 609
Size: 52 416 141
Fragments arranged by size:
416 141 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 609
Size: 261 348
EcoO109I rG'GnC_Cy
Cuts at: 0 315 609
Size: 315 294
FspI TGC'GCA
Cuts at: 0 445 609
Size: 445 164
HaeII r_GCGC'y
Cuts at: 0 35 96 152 367 459 583 609
Size: 35 61 56 215 92 124 26
Fragments arranged by size:
215 124 92 61 56 35 26
KasI G'GCGC_C
Cuts at: 0 31 148 609
Size: 31 117 461
Fragments arranged by size:
461 117 31
MscI TGG'CCA
Cuts at: 0 225 609
Size: 225 384
MslI CAynn'nnrTG
Cuts at: 0 373 609
Size: 373 236
MspA1I CmG'CkG
Cuts at: 0 133 402 420 609
Size: 133 269 18 189
Fragments arranged by size:
269 189 133 18
NaeI GCC'GGC
Cuts at: 0 347 609
Size: 347 262
NarI GG'CG_CC
Cuts at: 0 32 149 609
Size: 32 117 460
Fragments arranged by size:
460 117 32
NgoMIV G'CCGG_C
Cuts at: 0 345 609
Size: 345 264
PmlI CAC'GTG
Cuts at: 0 498 609
Size: 498 111
PspXI vC'TCGA_Gb
Cuts at: 0 198 469 609
Size: 198 271 140
Fragments arranged by size:
271 198 140
PstI C_TGCA'G
Cuts at: 0 289 575 601 609
Size: 289 286 26 8
Fragments arranged by size:
289 286 26 8
RsrII CG'GwC_CG
Cuts at: 0 415 609
Size: 415 194
SacII CC_GC'GG
Cuts at: 0 134 421 609
Size: 134 287 188
Fragments arranged by size:
287 188 134
SbfI CC_TGCA'GG
Cuts at: 0 575 609
Size: 575 34
SfcI C'TryA_G
Cuts at: 0 285 571 597 609
Size: 285 286 26 12
Fragments arranged by size:
286 285 26 12
SfoI GGC'GCC
Cuts at: 0 33 150 609
Size: 33 117 459
Fragments arranged by size:
459 117 33
SmaI CCC'GGG
Cuts at: 0 38 207 609
Size: 38 169 402
Fragments arranged by size:
402 169 38
SmlI C'TyrA_G
Cuts at: 0 162 198 469 609
Size: 162 36 271 140
Fragments arranged by size:
271 162 140 36
TatI w'GTAC_w
Cuts at: 0 485 609
Size: 485 124
XhoI C'TCGA_G
Cuts at: 0 198 469 609
Size: 198 271 140
Fragments arranged by size:
271 198 140
XmaI C'CCGG_G
Cuts at: 0 36 205 609
Size: 36 169 404
Fragments arranged by size:
404 169 36
ZraI GAC'GTC
Cuts at: 0 183 609
Size: 183 426
Enzymes that do cut:
AatII AccI AlwNI AscI AvaI BanI BanII BbeI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrFI BssHII BssSI
BstAPI BstXI BtgI BtgZI DrdI EaeI EarI EciI
Eco57MI EcoO109I FspI HaeII KasI MscI MslI MspA1I
NaeI NarI NgoMIV PmlI PspXI PstI RsrII SacII
SbfI SfcI SfoI SmaI SmlI TatI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaBI BsaXI BseYI BsiEI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrGI BstBI BstEII BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI PvuII SacI SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XmnI