Restriction map for
pF1KB8642
(Linear) (Six-base) MAPSORT of: KIBB8642.seq Check: 1048 from: 1 to: 3273
KIBB8642.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 3056 3273
Size: 3056 217
AclI AA'CG_TT
Cuts at: 0 768 2973 3273
Size: 768 2205 300
Fragments arranged by size:
2205 768 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1618 2038 2438 3121 3273
Size: 1618 420 400 683 152
Fragments arranged by size:
1618 683 420 400 152
AflII C'TTAA_G
Cuts at: 0 2389 3273
Size: 2389 884
AlwNI CAG_nnn'CTG
Cuts at: 0 559 3273
Size: 559 2714
ApoI r'AATT_y
Cuts at: 0 21 622 1272 2168 2350 2874 3046 3188
Size: 21 601 650 896 182 524 172 142
Cuts at: 3188 3273
Size: 85
Fragments arranged by size:
896 650 601 524 182 172 142 85
21
AseI AT'TA_AT
Cuts at: 0 2360 2612 3273
Size: 2360 252 661
Fragments arranged by size:
2360 661 252
BamHI G'GATC_C
Cuts at: 0 307 3273
Size: 307 2966
BanI G'GyrC_C
Cuts at: 0 2114 3273
Size: 2114 1159
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 808 3273
Size: 101 707 2465
Fragments arranged by size:
2465 707 101
BbvCI CC'TCA_GC
Cuts at: 0 1088 3273
Size: 1088 2185
BciVI GTATCCnnnnn_n'
Cuts at: 0 2472 3273
Size: 2472 801
BclI T'GATC_A
Cuts at: 0 525 1923 3273
Size: 525 1398 1350
Fragments arranged by size:
1398 1350 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 2764 3273
Size: 2764 509
BmtI G_CTAG'C
Cuts at: 0 2380 3273
Size: 2380 893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2939 2984 3273
Size: 2939 45 289
Fragments arranged by size:
2939 289 45
Bpu10I CC'TnA_GC
Cuts at: 0 1088 1978 2234 3273
Size: 1088 890 256 1039
Fragments arranged by size:
1088 1039 890 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2200 3233 3273
Size: 2200 1033 40
Fragments arranged by size:
2200 1033 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 2692 3273
Size: 2692 581
BsaBI GATnn'nnATC
Cuts at: 0 1922 3273
Size: 1922 1351
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2524 2554 3273
Size: 2524 30 719
Fragments arranged by size:
2524 719 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 392 2545 3273
Size: 392 2153 728
Fragments arranged by size:
2153 728 392
BseYI C'CCAG_C
Cuts at: 0 3116 3273
Size: 3116 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1883 3273
Size: 269 1614 1390
Fragments arranged by size:
1614 1390 269
BsiHKAI G_wGCw'C
Cuts at: 0 1029 2774 3273
Size: 1029 1745 499
Fragments arranged by size:
1745 1029 499
BsmI GAATG_Cn'
Cuts at: 0 876 1532 3273
Size: 876 656 1741
Fragments arranged by size:
1741 876 656
Bsp1286I G_dGCh'C
Cuts at: 0 1029 2774 3273
Size: 1029 1745 499
Fragments arranged by size:
1745 1029 499
BspHI T'CATG_A
Cuts at: 0 629 1643 3273
Size: 629 1014 1630
Fragments arranged by size:
1630 1014 629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 933 3273
Size: 933 2340
BsrBI CCG'CTC
Cuts at: 0 726 3273
Size: 726 2547
BsrDI GCAATG_nn'
Cuts at: 0 3226 3273
Size: 3226 47
BssSI C'ACGA_G
Cuts at: 0 495 1022 1859 3273
Size: 495 527 837 1414
Fragments arranged by size:
1414 837 527 495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2644 3273
Size: 2644 629
BstYI r'GATC_y
Cuts at: 0 307 3273
Size: 307 2966
BtgI C'CryG_G
Cuts at: 0 485 3086 3273
Size: 485 2601 187
Fragments arranged by size:
2601 485 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3000 3273
Size: 3000 273
BtsI GCAGTG_nn'
Cuts at: 0 848 3273
Size: 848 2425
ClaI AT'CG_AT
Cuts at: 0 2616 3273
Size: 2616 657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 219 254 3273
Size: 219 35 3019
Fragments arranged by size:
3019 219 35
DraI TTT'AAA
Cuts at: 0 317 393 2735 3273
Size: 317 76 2342 538
Fragments arranged by size:
2342 538 317 76
DraIII CAC_nnn'GTG
Cuts at: 0 2990 3273
Size: 2990 283
EaeI y'GGCC_r
Cuts at: 0 2 1675 3273
Size: 2 1673 1598
Fragments arranged by size:
1673 1598 2
EarI CTCTTCn'nnn_
Cuts at: 0 238 279 500 716 1004 1160 1463 3273
Size: 238 41 221 216 288 156 303 1810
Fragments arranged by size:
1810 303 288 238 221 216 156 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1618 2038 2438 2939 2984 3121 3273
Size: 1618 420 400 501 45 137 152
Fragments arranged by size:
1618 501 420 400 152 137 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2921 3212 3273
Size: 2921 291 61
Fragments arranged by size:
2921 291 61
EcoO109I rG'GnC_Cy
Cuts at: 0 1073 1516 1545 1629 1667 3273
Size: 1073 443 29 84 38 1606
Fragments arranged by size:
1606 1073 443 84 38 29
EcoRI G'AATT_C
Cuts at: 0 3188 3273
Size: 3188 85
EcoRV GAT'ATC
Cuts at: 0 354 3273
Size: 354 2919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 473 606 745 3273
Size: 473 133 139 2528
Fragments arranged by size:
2528 473 139 133
HaeII r_GCGC'y
Cuts at: 0 747 3273
Size: 747 2526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 872 904 1368 1400 2034 2066 2524 2556
Size: 872 32 464 32 634 32 458 32
Cuts at: 2556 3119 3151 3273
Size: 563 32 122
Fragments arranged by size:
872 634 563 464 458 122 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 213 3273
Size: 213 3060
HindIII A'AGCT_T
Cuts at: 0 1904 3273
Size: 1904 1369
MfeI C'AATT_G
Cuts at: 0 865 3273
Size: 865 2408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1190 1777 3273
Size: 114 1076 587 1496
Fragments arranged by size:
1496 1076 587 114
MscI TGG'CCA
Cuts at: 0 4 3273
Size: 4 3269
MslI CAynn'nnrTG
Cuts at: 0 541 1032 1613 1619 2888 3064 3273
Size: 541 491 581 6 1269 176 209
Fragments arranged by size:
1269 581 541 491 209 176 6
MspA1I CmG'CkG
Cuts at: 0 2853 3273
Size: 2853 420
NcoI C'CATG_G
Cuts at: 0 3086 3273
Size: 3086 187
NdeI CA'TA_TG
Cuts at: 0 2273 3273
Size: 2273 1000
NheI G'CTAG_C
Cuts at: 0 2376 3273
Size: 2376 897
NspI r_CATG'y
Cuts at: 0 1372 2647 3273
Size: 1372 1275 626
Fragments arranged by size:
1372 1275 626
PflMI CCAn_nnn'nTGG
Cuts at: 0 1194 3273
Size: 1194 2079
PpuMI rG'GwC_Cy
Cuts at: 0 1545 1629 1667 3273
Size: 1545 84 38 1606
Fragments arranged by size:
1606 1545 84 38
PsiI TTA'TAA
Cuts at: 0 461 2129 3273
Size: 461 1668 1144
Fragments arranged by size:
1668 1144 461
PstI C_TGCA'G
Cuts at: 0 126 351 1595 3273
Size: 126 225 1244 1678
Fragments arranged by size:
1678 1244 225 126
SanDI GG'GwC_CC
Cuts at: 0 1545 3273
Size: 1545 1728
SapI GCTCTTCn'nnn_
Cuts at: 0 279 500 716 1160 1463 3273
Size: 279 221 216 444 303 1810
Fragments arranged by size:
1810 444 303 279 221 216
ScaI AGT'ACT
Cuts at: 0 254 1182 3273
Size: 254 928 2091
Fragments arranged by size:
2091 928 254
SfcI C'TryA_G
Cuts at: 0 122 347 1511 1591 1853 2204 3273
Size: 122 225 1164 80 262 351 1069
Fragments arranged by size:
1164 1069 351 262 225 122 80
SmlI C'TyrA_G
Cuts at: 0 2179 2389 3248 3273
Size: 2179 210 859 25
Fragments arranged by size:
2179 859 210 25
SphI G_CATG'C
Cuts at: 0 1372 3273
Size: 1372 1901
SspI AAT'ATT
Cuts at: 0 2160 3273
Size: 2160 1113
StuI AGG'CCT
Cuts at: 0 1255 1597 3273
Size: 1255 342 1676
Fragments arranged by size:
1676 1255 342
StyI C'CwwG_G
Cuts at: 0 3086 3273
Size: 3086 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 1902 3273
Size: 262 1640 1371
Fragments arranged by size:
1640 1371 262
TatI w'GTAC_w
Cuts at: 0 252 793 971 1180 3273
Size: 252 541 178 209 2093
Fragments arranged by size:
2093 541 252 209 178
XmnI GAAnn'nnTTC
Cuts at: 0 770 1298 3273
Size: 770 528 1975
Fragments arranged by size:
1975 770 528
Enzymes that do cut:
AccI AclI AcuI AflII AlwNI ApoI AseI BamHI
BanI BbsI BbvCI BciVI BclI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI
BstAPI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NheI NspI PflMI PpuMI
PsiI PstI SanDI SapI ScaI SfcI SmlI SphI
SspI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanII BbeI BcgI BfrBI BglI BglII BlpI
Bme1580I BmgBI BplI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I DrdI EagI EciI EcoICRI FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI