Restriction map for
pF1KB8643
(Linear) (Six-base) MAPSORT of: KIBB8643.seq Check: 1724 from: 1 to: 1551
KIBB8643.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 1043 1551
Size: 1043 508
AccI GT'mk_AC
Cuts at: 0 728 1551
Size: 728 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 887 1551
Size: 343 544 664
Fragments arranged by size:
664 544 343
AfeI AGC'GCT
Cuts at: 0 566 1551
Size: 566 985
AflIII A'CryG_T
Cuts at: 0 71 443 1364 1551
Size: 71 372 921 187
Fragments arranged by size:
921 372 187 71
AleI CACnn'nnGTG
Cuts at: 0 154 1551
Size: 154 1397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 629 1551
Size: 629 922
AlwNI CAG_nnn'CTG
Cuts at: 0 1449 1551
Size: 1449 102
ApaLI G'TGCA_C
Cuts at: 0 1207 1551
Size: 1207 344
AvaI C'yCGr_G
Cuts at: 0 54 376 1551
Size: 54 322 1175
Fragments arranged by size:
1175 322 54
BanI G'GyrC_C
Cuts at: 0 79 1214 1287 1551
Size: 79 1135 73 264
Fragments arranged by size:
1135 264 79 73
BanII G_rGCy'C
Cuts at: 0 242 865 1551
Size: 242 623 686
Fragments arranged by size:
686 623 242
BbsI GAAGACnn'nnnn_
Cuts at: 0 987 1551
Size: 987 564
BciVI GTATCCnnnnn_n'
Cuts at: 0 943 1551
Size: 943 608
BglI GCCn_nnn'nGGC
Cuts at: 0 253 1551
Size: 253 1298
BlpI GC'TnA_GC
Cuts at: 0 561 568 1551
Size: 561 7 983
Fragments arranged by size:
983 561 7
Bme1580I G_kGCm'C
Cuts at: 0 84 1055 1211 1292 1551
Size: 84 971 156 81 259
Fragments arranged by size:
971 259 156 84 81
BmgBI CAC'GTC
Cuts at: 0 446 692 1551
Size: 446 246 859
Fragments arranged by size:
859 446 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 489 1473 1551
Size: 489 984 78
Fragments arranged by size:
984 489 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 1551
Size: 408 1143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1426 1551
Size: 733 693 125
Fragments arranged by size:
733 693 125
Bpu10I CC'TnA_GC
Cuts at: 0 124 671 1551
Size: 124 547 880
Fragments arranged by size:
880 547 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1495 1551
Size: 655 840 56
Fragments arranged by size:
840 655 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 729 1551
Size: 729 822
BsaAI yAC'GTr
Cuts at: 0 72 1365 1551
Size: 72 1293 186
Fragments arranged by size:
1293 186 72
BsaHI Gr'CG_yC
Cuts at: 0 1040 1529 1551
Size: 1040 489 22
Fragments arranged by size:
1040 489 22
BsaWI w'CCGG_w
Cuts at: 0 661 731 1335 1551
Size: 661 70 604 216
Fragments arranged by size:
661 604 216 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 691 1036 1551
Size: 691 345 515
Fragments arranged by size:
691 515 345
BseYI C'CCAG_C
Cuts at: 0 83 220 400 525 544 939 1551
Size: 83 137 180 125 19 395 612
Fragments arranged by size:
612 395 180 137 125 83 19
BsiHKAI G_wGCw'C
Cuts at: 0 242 788 1211 1551
Size: 242 546 423 340
Fragments arranged by size:
546 423 340 242
BsmI GAATG_Cn'
Cuts at: 0 1118 1551
Size: 1118 433
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1047 1551
Size: 280 767 504
Fragments arranged by size:
767 504 280
Bsp1286I G_dGCh'C
Cuts at: 0 84 242 788 865 1055 1211 1292 1551
Size: 84 158 546 77 190 156 81 259
Fragments arranged by size:
546 259 190 158 156 84 81 77
BspEI T'CCGG_A
Cuts at: 0 661 1551
Size: 661 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1250 1280 1457 1551
Size: 1250 30 177 94
Fragments arranged by size:
1250 177 94 30
BsrFI r'CCGG_y
Cuts at: 0 616 1211 1551
Size: 616 595 340
Fragments arranged by size:
616 595 340
BssSI C'ACGA_G
Cuts at: 0 1183 1381 1551
Size: 1183 198 170
Fragments arranged by size:
1183 198 170
BstAPI GCAn_nnn'nTGC
Cuts at: 0 463 1551
Size: 463 1088
BstEII G'GTnAC_C
Cuts at: 0 709 1551
Size: 709 842
BstXI CCAn_nnnn'nTGG
Cuts at: 0 692 1551
Size: 692 859
BstYI r'GATC_y
Cuts at: 0 836 911 1551
Size: 836 75 640
Fragments arranged by size:
836 640 75
BtgI C'CryG_G
Cuts at: 0 131 248 1551
Size: 131 117 1303
Fragments arranged by size:
1303 131 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 221 485 1551
Size: 221 264 1066
Fragments arranged by size:
1066 264 221
DraIII CAC_nnn'GTG
Cuts at: 0 1233 1551
Size: 1233 318
EaeI y'GGCC_r
Cuts at: 0 2 134 245 290 512 1493 1551
Size: 2 132 111 45 222 981 58
Fragments arranged by size:
981 222 132 111 58 45 2
EarI CTCTTCn'nnn_
Cuts at: 0 1441 1551
Size: 1441 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 922 1297 1551
Size: 271 651 375 254
Fragments arranged by size:
651 375 271 254
EcoICRI GAG'CTC
Cuts at: 0 240 1551
Size: 240 1311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 733 887 1426 1551
Size: 343 390 154 539 125
Fragments arranged by size:
539 390 343 154 125
EcoO109I rG'GnC_Cy
Cuts at: 0 491 1551
Size: 491 1060
HaeII r_GCGC'y
Cuts at: 0 568 1551
Size: 568 983
MscI TGG'CCA
Cuts at: 0 4 247 1551
Size: 4 243 1304
Fragments arranged by size:
1304 243 4
MslI CAynn'nnrTG
Cuts at: 0 74 154 1466 1551
Size: 74 80 1312 85
Fragments arranged by size:
1312 85 80 74
MspA1I CmG'CkG
Cuts at: 0 321 404 609 1259 1551
Size: 321 83 205 650 292
Fragments arranged by size:
650 321 292 205 83
NaeI GCC'GGC
Cuts at: 0 618 1551
Size: 618 933
NcoI C'CATG_G
Cuts at: 0 248 1551
Size: 248 1303
NdeI CA'TA_TG
Cuts at: 0 922 1551
Size: 922 629
NgoMIV G'CCGG_C
Cuts at: 0 616 1551
Size: 616 935
PflMI CCAn_nnn'nTGG
Cuts at: 0 946 1551
Size: 946 605
PfoI T'CCnGG_A
Cuts at: 0 182 1551
Size: 182 1369
PmlI CAC'GTG
Cuts at: 0 72 1551
Size: 72 1479
PpuMI rG'GwC_Cy
Cuts at: 0 491 1551
Size: 491 1060
PstI C_TGCA'G
Cuts at: 0 344 459 488 884 1247 1551
Size: 344 115 29 396 363 304
Fragments arranged by size:
396 363 344 304 115 29
PvuII CAG'CTG
Cuts at: 0 609 1551
Size: 609 942
SacI G_AGCT'C
Cuts at: 0 242 1551
Size: 242 1309
SapI GCTCTTCn'nnn_
Cuts at: 0 1441 1551
Size: 1441 110
SfcI C'TryA_G
Cuts at: 0 340 455 484 880 1243 1551
Size: 340 115 29 396 363 308
Fragments arranged by size:
396 363 340 308 115 29
SmaI CCC'GGG
Cuts at: 0 56 1551
Size: 56 1495
SmlI C'TyrA_G
Cuts at: 0 634 1510 1551
Size: 634 876 41
Fragments arranged by size:
876 634 41
SspI AAT'ATT
Cuts at: 0 769 1551
Size: 769 782
StuI AGG'CCT
Cuts at: 0 583 1501 1551
Size: 583 918 50
Fragments arranged by size:
918 583 50
StyI C'CwwG_G
Cuts at: 0 248 887 1551
Size: 248 639 664
Fragments arranged by size:
664 639 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 517 953 1551
Size: 417 100 436 598
Fragments arranged by size:
598 436 417 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 435 467 1551
Size: 435 32 1084
Fragments arranged by size:
1084 435 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 73 522 943 1551
Size: 73 449 421 608
Fragments arranged by size:
608 449 421 73
XmaI C'CCGG_G
Cuts at: 0 54 1551
Size: 54 1497
XmnI GAAnn'nnTTC
Cuts at: 0 25 1551
Size: 25 1526
ZraI GAC'GTC
Cuts at: 0 1041 1551
Size: 1041 510
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaLI AvaI BanI BanII BbsI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrFI BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV PflMI PfoI PmlI PpuMI PstI PvuII
SacI SapI SfcI SmaI SmlI SspI StuI StyI
TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BfrBI BglII BmtI BsaBI BsaXI
BsgI BsiEI BsiWI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TatI Tth111I XbaI XhoI