Restriction map for
pF1KB8647
(Linear) (Six-base) MAPSORT of: KIBB8647.seq Check: 9430 from: 1 to: 2472
KIBB8647.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 427 725 2472
Size: 427 298 1747
Fragments arranged by size:
1747 427 298
AccI GT'mk_AC
Cuts at: 0 1767 2423 2472
Size: 1767 656 49
Fragments arranged by size:
1767 656 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 2472
Size: 697 1775
AflIII A'CryG_T
Cuts at: 0 2170 2472
Size: 2170 302
AhdI GACnn_n'nnGTC
Cuts at: 0 1381 2472
Size: 1381 1091
AleI CACnn'nnGTG
Cuts at: 0 835 1775 2440 2472
Size: 835 940 665 32
Fragments arranged by size:
940 835 665 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1504 2212 2472
Size: 1504 708 260
Fragments arranged by size:
1504 708 260
AlwNI CAG_nnn'CTG
Cuts at: 0 492 2232 2472
Size: 492 1740 240
Fragments arranged by size:
1740 492 240
ApaI G_GGCC'C
Cuts at: 0 727 812 1301 1690 1788 2472
Size: 727 85 489 389 98 684
Fragments arranged by size:
727 684 489 389 98 85
ApaLI G'TGCA_C
Cuts at: 0 1161 1312 2472
Size: 1161 151 1160
Fragments arranged by size:
1161 1160 151
ApoI r'AATT_y
Cuts at: 0 618 2472
Size: 618 1854
AvaI C'yCGr_G
Cuts at: 0 113 448 475 568 2472
Size: 113 335 27 93 1904
Fragments arranged by size:
1904 335 113 93 27
AvrII C'CTAG_G
Cuts at: 0 198 2472
Size: 198 2274
BamHI G'GATC_C
Cuts at: 0 1235 2453 2472
Size: 1235 1218 19
Fragments arranged by size:
1235 1218 19
BanI G'GyrC_C
Cuts at: 0 132 713 1076 1086 1557 2095 2110 2472
Size: 132 581 363 10 471 538 15 362
Fragments arranged by size:
581 538 471 363 362 132 15 10
BanII G_rGCy'C
Cuts at: 0 347 475 727 812 863 1301 1690 1788
Size: 347 128 252 85 51 438 389 98
Cuts at: 1788 2066 2472
Size: 278 406
Fragments arranged by size:
438 406 389 347 278 252 128 98
85 51
BbeI G_GCGC'C
Cuts at: 0 2099 2114 2472
Size: 2099 15 358
Fragments arranged by size:
2099 358 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 957 2472
Size: 957 1515
BbvCI CC'TCA_GC
Cuts at: 0 1024 2472
Size: 1024 1448
BclI T'GATC_A
Cuts at: 0 2444 2472
Size: 2444 28
BfrBI ATG'CAT
Cuts at: 0 1481 2472
Size: 1481 991
BglI GCCn_nnn'nGGC
Cuts at: 0 895 1418 1685 2119 2472
Size: 895 523 267 434 353
Fragments arranged by size:
895 523 434 353 267
BglII A'GATC_T
Cuts at: 0 1283 2472
Size: 1283 1189
BlpI GC'TnA_GC
Cuts at: 0 469 2472
Size: 469 2003
Bme1580I G_kGCm'C
Cuts at: 0 137 727 812 1091 1165 1301 1316 1690
Size: 137 590 85 279 74 136 15 374
Cuts at: 1690 1788 2472
Size: 98 684
Fragments arranged by size:
684 590 374 279 137 136 98 85
74 15
BmgBI CAC'GTC
Cuts at: 0 801 1977 2472
Size: 801 1176 495
Fragments arranged by size:
1176 801 495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1375 2472
Size: 1375 1097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 684 775 835 1370 1666 1677 1738
Size: 366 318 91 60 535 296 11 61
Cuts at: 1738 1925 2472
Size: 187 547
Fragments arranged by size:
547 535 366 318 296 187 91 61
60 11
Bpu10I CC'TnA_GC
Cuts at: 0 1024 2472
Size: 1024 1448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 2472
Size: 322 2150
BsaAI yAC'GTr
Cuts at: 0 651 2472
Size: 651 1821
BsaBI GATnn'nnATC
Cuts at: 0 1264 2472
Size: 1264 1208
BsaHI Gr'CG_yC
Cuts at: 0 880 1019 2096 2111 2263 2420 2472
Size: 880 139 1077 15 152 157 52
Fragments arranged by size:
1077 880 157 152 139 52 15
BsaWI w'CCGG_w
Cuts at: 0 1592 2472
Size: 1592 880
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 370 394 400 496 526 682 712
Size: 364 6 24 6 96 30 156 30
Cuts at: 712 1185 1215 1261 1291 2472
Size: 473 30 46 30 1181
Fragments arranged by size:
1181 473 364 156 96 46 30 30
30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 1152 1675 2472
Size: 517 635 523 797
Fragments arranged by size:
797 635 523 517
BseYI C'CCAG_C
Cuts at: 0 627 1042 1802 2090 2384 2472
Size: 627 415 760 288 294 88
Fragments arranged by size:
760 627 415 294 288 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 904 973 1924 2056 2233 2256 2472
Size: 505 399 69 951 132 177 23 216
Fragments arranged by size:
951 505 399 216 177 132 69 23
BsiEI CG_ry'CG
Cuts at: 0 902 1256 2018 2472
Size: 902 354 762 454
Fragments arranged by size:
902 762 454 354
BsiHKAI G_wGCw'C
Cuts at: 0 347 455 947 1165 1316 1667 2472
Size: 347 108 492 218 151 351 805
Fragments arranged by size:
805 492 351 347 218 151 108
Bsp1286I G_dGCh'C
Cuts at: 0 137 347 455 475 727 812 863 947
Size: 137 210 108 20 252 85 51 84
Cuts at: 947 1091 1165 1301 1316 1667 1690 1788 2066
Size: 144 74 136 15 351 23 98 278
Cuts at: 2066 2472
Size: 406
Fragments arranged by size:
406 351 278 252 210 144 137 136
108 98 85 84 74 51 23 20
15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 725 2472
Size: 427 298 1747
Fragments arranged by size:
1747 427 298
BsrFI r'CCGG_y
Cuts at: 0 458 957 2281 2402 2472
Size: 458 499 1324 121 70
Fragments arranged by size:
1324 499 458 121 70
BsrGI T'GTAC_A
Cuts at: 0 1325 2472
Size: 1325 1147
BssHII G'CGCG_C
Cuts at: 0 2044 2472
Size: 2044 428
BssSI C'ACGA_G
Cuts at: 0 160 2472
Size: 160 2312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 2472
Size: 492 1980
BstEII G'GTnAC_C
Cuts at: 0 2466 2472
Size: 2466 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 28 1422 2472
Size: 28 1394 1050
Fragments arranged by size:
1394 1050 28
BstYI r'GATC_y
Cuts at: 0 276 1235 1283 2453 2472
Size: 276 959 48 1170 19
Fragments arranged by size:
1170 959 276 48 19
BtgI C'CryG_G
Cuts at: 0 5 863 1241 1250 1738 1850 2472
Size: 5 858 378 9 488 112 622
Fragments arranged by size:
858 622 488 378 112 9 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1776 2472
Size: 1776 696
BtsI GCAGTG_nn'
Cuts at: 0 315 490 1065 1486 1549 1627 1821 2073
Size: 315 175 575 421 63 78 194 252
Cuts at: 2073 2472
Size: 399
Fragments arranged by size:
575 421 399 315 252 194 175 78
63
DraIII CAC_nnn'GTG
Cuts at: 0 2004 2353 2472
Size: 2004 349 119
Fragments arranged by size:
2004 349 119
EaeI y'GGCC_r
Cuts at: 0 8 788 803 1247 1253 1735 2015 2355
Size: 8 780 15 444 6 482 280 340
Cuts at: 2355 2400 2472
Size: 45 72
Fragments arranged by size:
780 482 444 340 280 72 45 15
8 6
EagI C'GGCC_G
Cuts at: 0 1253 2015 2472
Size: 1253 762 457
Fragments arranged by size:
1253 762 457
EarI CTCTTCn'nnn_
Cuts at: 0 814 1532 1949 2472
Size: 814 718 417 523
Fragments arranged by size:
814 718 523 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 226 2137 2472
Size: 115 111 1911 335
Fragments arranged by size:
1911 335 115 111
EcoICRI GAG'CTC
Cuts at: 0 345 2472
Size: 345 2127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 684 697 775 835 1370 1666 1677
Size: 366 318 13 78 60 535 296 11
Cuts at: 1677 1738 1925 2472
Size: 61 187 547
Fragments arranged by size:
547 535 366 318 296 187 78 61
60 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1876 2472
Size: 1876 596
EcoO109I rG'GnC_Cy
Cuts at: 0 15 409 573 666 682 723 724 732
Size: 15 394 164 93 16 41 1 8
Cuts at: 732 809 915 1686 1687 1784 1785 2229 2388
Size: 77 106 771 1 97 1 444 159
Cuts at: 2388 2472
Size: 84
Fragments arranged by size:
771 444 394 164 159 106 97 93
84 77 41 16 15 8 1 1
1
EcoRI G'AATT_C
Cuts at: 0 618 2472
Size: 618 1854
FseI GG_CCGG'CC
Cuts at: 0 2406 2472
Size: 2406 66
FspI TGC'GCA
Cuts at: 0 1210 2472
Size: 1210 1262
HaeII r_GCGC'y
Cuts at: 0 1984 2099 2114 2472
Size: 1984 115 15 358
Fragments arranged by size:
1984 358 115 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 1367 1399 2472
Size: 496 32 839 32 1073
Fragments arranged by size:
1073 839 496 32 32
HincII GTy'rAC
Cuts at: 0 522 1768 2472
Size: 522 1246 704
Fragments arranged by size:
1246 704 522
KasI G'GCGC_C
Cuts at: 0 2095 2110 2472
Size: 2095 15 362
Fragments arranged by size:
2095 362 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 2472
Size: 1316 1156
MscI TGG'CCA
Cuts at: 0 790 1249 1737 2472
Size: 790 459 488 735
Fragments arranged by size:
790 735 488 459
MslI CAynn'nnrTG
Cuts at: 0 835 1458 1775 2440 2472
Size: 835 623 317 665 32
Fragments arranged by size:
835 665 623 317 32
MspA1I CmG'CkG
Cuts at: 0 704 1802 1816 2334 2360 2384 2450 2472
Size: 704 1098 14 518 26 24 66 22
Fragments arranged by size:
1098 704 518 66 26 24 22 14
NaeI GCC'GGC
Cuts at: 0 460 2283 2404 2472
Size: 460 1823 121 68
Fragments arranged by size:
1823 460 121 68
NarI GG'CG_CC
Cuts at: 0 2096 2111 2472
Size: 2096 15 361
Fragments arranged by size:
2096 361 15
NcoI C'CATG_G
Cuts at: 0 1738 2472
Size: 1738 734
NgoMIV G'CCGG_C
Cuts at: 0 458 2281 2402 2472
Size: 458 1823 121 70
Fragments arranged by size:
1823 458 121 70
NsiI A_TGCA'T
Cuts at: 0 1483 2472
Size: 1483 989
NspI r_CATG'y
Cuts at: 0 1481 2174 2274 2472
Size: 1481 693 100 198
Fragments arranged by size:
1481 693 198 100
PasI CC'CwG_GG
Cuts at: 0 1014 1167 2472
Size: 1014 153 1305
Fragments arranged by size:
1305 1014 153
PciI A'CATG_T
Cuts at: 0 2170 2472
Size: 2170 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 2004 2463 2472
Size: 2004 459 9
Fragments arranged by size:
2004 459 9
PfoI T'CCnGG_A
Cuts at: 0 584 1829 2472
Size: 584 1245 643
Fragments arranged by size:
1245 643 584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 2472
Size: 64 32 2376
Fragments arranged by size:
2376 64 32
PpuMI rG'GwC_Cy
Cuts at: 0 409 666 732 2229 2472
Size: 409 257 66 1497 243
Fragments arranged by size:
1497 409 257 243 66
PspOMI G'GGCC_C
Cuts at: 0 723 808 1297 1686 1784 2472
Size: 723 85 489 389 98 688
Fragments arranged by size:
723 688 489 389 98 85
PstI C_TGCA'G
Cuts at: 0 722 850 1220 1645 1991 2201 2472
Size: 722 128 370 425 346 210 271
Fragments arranged by size:
722 425 370 346 271 210 128
PvuII CAG'CTG
Cuts at: 0 704 1816 2384 2450 2472
Size: 704 1112 568 66 22
Fragments arranged by size:
1112 704 568 66 22
SacI G_AGCT'C
Cuts at: 0 347 2472
Size: 347 2125
SalI G'TCGA_C
Cuts at: 0 1766 2472
Size: 1766 706
SanDI GG'GwC_CC
Cuts at: 0 409 2472
Size: 409 2063
SapI GCTCTTCn'nnn_
Cuts at: 0 1532 2472
Size: 1532 940
SbfI CC_TGCA'GG
Cuts at: 0 722 2472
Size: 722 1750
SexAI A'CCwGG_T
Cuts at: 0 2255 2472
Size: 2255 217
SfcI C'TryA_G
Cuts at: 0 543 642 718 846 1216 1641 1987 2197
Size: 543 99 76 128 370 425 346 210
Cuts at: 2197 2472
Size: 275
Fragments arranged by size:
543 425 370 346 275 210 128 99
76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1685 2472
Size: 1685 787
SfoI GGC'GCC
Cuts at: 0 2097 2112 2472
Size: 2097 15 360
Fragments arranged by size:
2097 360 15
SmaI CCC'GGG
Cuts at: 0 115 2472
Size: 115 2357
SmlI C'TyrA_G
Cuts at: 0 337 2472
Size: 337 2135
SphI G_CATG'C
Cuts at: 0 2274 2472
Size: 2274 198
StuI AGG'CCT
Cuts at: 0 88 973 2472
Size: 88 885 1499
Fragments arranged by size:
1499 885 88
StyI C'CwwG_G
Cuts at: 0 198 728 1493 1738 2157 2472
Size: 198 530 765 245 419 315
Fragments arranged by size:
765 530 419 315 245 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 797 1180 1794 2472
Size: 797 383 614 678
Fragments arranged by size:
797 678 614 383
TatI w'GTAC_w
Cuts at: 0 233 252 1325 2472
Size: 233 19 1073 1147
Fragments arranged by size:
1147 1073 233 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 1185 1217 2472
Size: 682 32 471 32 1255
Fragments arranged by size:
1255 682 471 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 798 2472
Size: 360 438 1674
Fragments arranged by size:
1674 438 360
XmaI C'CCGG_G
Cuts at: 0 113 2472
Size: 113 2359
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI BaeI BcgI BciVI BmrI BmtI BsaI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FalI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI PacI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI